Rw1G001390

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008425
Physical Location & Seq
Reverse (-)
2318497 .. 2323310
4814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G001390.1

Sequence Viewer

Length: 1275 bp
ATGAAGCTTGCAACTTGTTCATATTCGTCGTCACCAATCTCCGTTACTCCTTTTCTCCTTCAAACCCGTCCCAGATTCTCATTCAATATATTTCAGGCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAACCGCCGCCCGCCAACCACGACCCGCAATGGTTTCCGCTCGCAACTACGCCCACGCCCACTACCTTCCTCCTTGGTTCAGTGTGGCTCCTATGATGGAATGGACCGATGTTCACTACAGGACTATGGCCCGCCTAATTTCCAAGCATGCCTGGCTCTACACTGAGATGCTCGCTGCCGAAACCATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCAGAACAGCATCCTATTGTTCTACAAATTGGTGGCAGTAACTTAGAAAACCTGGCAAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTCGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCCGAGGCTATGTCAGTGATTGCTGCGAACACAGATGTCCCTGTCAGCGTTAAGTGTCGAATTGGTGTTGATGATCACGATTCATATAATGATTTCTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCCATGCTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTTAAATACGAGTACTTTTATGGCCTCTTGCGTGACTTTCCGGACTTAAGATTTACCATAAATGGTGGAATAAACACTATTGATGAGGTCAATGCAGCTAGGAAAGCAGGAGCTCATGGTGTAATGGTTGGACGGGCTGCATTTAATAACTCGTGGAATACTTTGGGACATGTTGATACTGCTATTTATGGTGCACCAAGCAGCGGTATAACACGGCGTCAGTTACTCAAGGAGTTTGAAGCTTATGGAGAGACTGTTTTGGGAAAATATGGACAAAAGCCCAATGTTCGAGATGTGGCCAAGCCTCTGCTTAGCCTTTTCTATGCTCAGCCTGGGAACAGCCTTTGGAAACGCAAAGCTGATGCTGCTTTCATGCATTGCACGACTATGAAATCATTTTTTGAGGAAACACTAGTAGCAATTCCAGACTTTGTCTTGGATGCACCTGTTGGAGCGCCACCATCTGGTAGTAATGATCCTTTTTCAAACATACATACTTTGTTGCCTCCACCATACGAATCAAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

424

Amino Acids

47.18

Weight (kDa)

8.4

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 71 - 370 2e-53 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1190
AccBSI CCGCTC 1 cut(s) 167
AccIII TCCGGA 1 cut(s) 766
AciI CCGC 7 cut(s) 132, 135, 139, 153, 165, 259, 930
AclWI GGATC 3 cut(s) 521, 534, 1196
AcoI YGGCCR 1 cut(s) 1023
AcyI GRCGYC 1 cut(s) 943
AfaI GTAC 2 cut(s) 350, 740
AfiI CCNNNNNNNGG 2 cut(s) 929, 1190
AflII CTTAAG 1 cut(s) 772
AflIII ACRYGT 1 cut(s) 895
AgsI TTSAA 5 cut(s) 62, 85, 129, 965, 1212
AhlI ACTAGT 1 cut(s) 1138
AjnI CCWGG 3 cut(s) 278, 408, 1057
AluBI AGCT 9 cut(s) 7, 419, 491, 704, 824, 839, 968, 1085, 1261
AluI AGCT 9 cut(s) 7, 419, 491, 704, 824, 839, 968, 1085, 1261
Alw21I GWGCWC 3 cut(s) 514, 841, 922
Alw26I GTCTC 1 cut(s) 971
Alw44I GTGCAC 1 cut(s) 918
AlwI GGATC 3 cut(s) 521, 534, 1196
Aor13HI TCCGGA 1 cut(s) 766
AoxI GGCC 5 cut(s) 96, 255, 678, 748, 1023
ApaLI GTGCAC 1 cut(s) 918
ApeKI GCWGC 6 cut(s) 302, 563, 821, 863, 927, 1091
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AspLEI GCGC 1 cut(s) 1183
AspS9I GGNCC 2 cut(s) 231, 256
AsuHPI GGTGA 2 cut(s) 24, 636
AvaII GGWCC 1 cut(s) 231
BaeGI GKGCMC 1 cut(s) 922
BalI TGGCCA 1 cut(s) 1025
BamHI GGATCC 1 cut(s) 526
BanII GRGCYC 1 cut(s) 841
BauI CACGAG 1 cut(s) 877
Bbv12I GWGCWC 3 cut(s) 514, 841, 922
BbvI GCAGC 6 cut(s) 289, 550, 833, 850, 939, 1078
BccI CCATC 2 cut(s) 217, 1195
BceAI ACGGC 1 cut(s) 956
BciT130I CCWGG 3 cut(s) 280, 410, 1059
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 971
BcuI ACTAGT 1 cut(s) 1138
BfaI CTAG 3 cut(s) 651, 825, 1139
BfmI CTRYAG 1 cut(s) 244
BfoI RGCGCY 1 cut(s) 1184
BfrI CTTAAG 1 cut(s) 772
BisI GCNGC 7 cut(s) 135, 303, 564, 822, 864, 928, 1092
BlpI GCTNAGC 2 cut(s) 1037, 1053
BlsI GCNGC 7 cut(s) 136, 304, 565, 823, 865, 929, 1093
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 3 cut(s) 280, 410, 1059
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 2 cut(s) 231, 256
BmiI GGNNCC 2 cut(s) 216, 528
BmrFI CCNGG 3 cut(s) 280, 410, 1059
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 4 cut(s) 285, 376, 1078, 1156
BmuI ACTGGG 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 342
Bpu1102I GCTNAGC 2 cut(s) 1037, 1053
BpuEI CTTGAG 1 cut(s) 938
BsaHI GRCGYC 1 cut(s) 943
BsaJI CCNNGG 3 cut(s) 200, 543, 1058
BsaWI WCCGGW 1 cut(s) 766
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 2 cut(s) 929, 1190
Bse1I ACTGG 1 cut(s) 477
Bse3DI GCAATG 2 cut(s) 162, 1102
BseAI TCCGGA 1 cut(s) 766
BseBI CCWGG 3 cut(s) 280, 410, 1059
BseDI CCNNGG 3 cut(s) 200, 543, 1058
BseGI GGATG 3 cut(s) 367, 476, 1171
BseLI CCNNNNNNNGG 2 cut(s) 929, 1190
BseMI GCAATG 2 cut(s) 162, 1102
BseMII CTCAG 2 cut(s) 282, 1067
BseNI ACTGG 1 cut(s) 477
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 6 cut(s) 289, 550, 833, 850, 939, 1078
BseYI CCCAGC 1 cut(s) 700
BshFI GGCC 5 cut(s) 98, 257, 680, 750, 1025
BsiHKAI GWGCWC 3 cut(s) 514, 841, 922
BsiSI CCGG 1 cut(s) 767
BslFI GGGAC 4 cut(s) 54, 459, 563, 906
BslI CCNNNNNNNGG 2 cut(s) 929, 1190
BsmAI GTCTC 1 cut(s) 971
BsmFI GGGAC 4 cut(s) 54, 459, 563, 906
BsnI GGCC 5 cut(s) 98, 257, 680, 750, 1025
Bsp1286I GDGCHC 3 cut(s) 514, 841, 922
Bsp13I TCCGGA 1 cut(s) 766
Bsp143I GATC 4 cut(s) 453, 526, 613, 1201
Bsp1720I GCTNAGC 2 cut(s) 1037, 1053
BspACI CCGC 7 cut(s) 132, 135, 139, 153, 165, 259, 930
BspANI GGCC 5 cut(s) 98, 257, 680, 750, 1025
BspCNI CTCAG 2 cut(s) 283, 1066
BspEI TCCGGA 1 cut(s) 766
BspLI GGNNCC 2 cut(s) 216, 528
BspPI GGATC 3 cut(s) 521, 534, 1196
BspTI CTTAAG 1 cut(s) 772
BsrBI CCGCTC 1 cut(s) 167
BsrDI GCAATG 2 cut(s) 162, 1102
BsrI ACTGG 1 cut(s) 477
BssECI CCNNGG 3 cut(s) 200, 543, 1058
BssMI GATC 4 cut(s) 453, 526, 613, 1201
BssNI GRCGYC 1 cut(s) 943
BssSI CACGAG 1 cut(s) 877
BssT1I CCWWGG 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 877
Bst2UI CCWGG 3 cut(s) 280, 410, 1059
Bst4CI ACNGT 1 cut(s) 982
Bst6I CTCTTC 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 943
BstAFI CTTAAG 1 cut(s) 772
BstC8I GCNNGC 6 cut(s) 9, 139, 169, 259, 276, 300
BstDEI CTNAG 5 cut(s) 291, 400, 1037, 1053, 1272
BstF5I GGATG 3 cut(s) 367, 476, 1171
BstH2I RGCGCY 1 cut(s) 1184
BstHHI GCGC 1 cut(s) 1183
BstKTI GATC 4 cut(s) 456, 529, 616, 1204
BstMAI GTCTC 1 cut(s) 971
BstMBI GATC 4 cut(s) 453, 526, 613, 1201
BstMWI GCNNNNNNNGC 3 cut(s) 280, 830, 1091
BstNI CCWGG 3 cut(s) 280, 410, 1059
BstNSI RCATGY 2 cut(s) 278, 899
BstSCI CCNGG 3 cut(s) 278, 408, 1057
BstSFI CTRYAG 1 cut(s) 244
BstSLI GKGCMC 1 cut(s) 922
BstV1I GCAGC 6 cut(s) 289, 550, 833, 850, 939, 1078
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 5 cut(s) 98, 257, 680, 750, 1025
BtsCI GGATG 3 cut(s) 367, 476, 1171
BtsIMutI CAGTG 3 cut(s) 214, 288, 561
Cac8I GCNNGC 6 cut(s) 9, 139, 169, 259, 276, 300
CfoI GCGC 1 cut(s) 1183
Cfr13I GGNCC 2 cut(s) 231, 256
CseI GACGC 1 cut(s) 932
Csp6I GTAC 2 cut(s) 349, 739
CviAII CATG 6 cut(s) 275, 497, 682, 842, 896, 1099
CviQI GTAC 2 cut(s) 349, 739
DdeI CTNAG 5 cut(s) 291, 400, 1037, 1053, 1272
DpnI GATC 4 cut(s) 455, 528, 615, 1203
DpnII GATC 4 cut(s) 453, 526, 613, 1201
EaeI YGGCCR 1 cut(s) 1023
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 839
Eco130I CCWWGG 1 cut(s) 200
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 839
EcoRII CCWGG 3 cut(s) 278, 408, 1057
EcoT14I CCWWGG 1 cut(s) 200
EcoT22I ATGCAT 1 cut(s) 1104
EcoT38I GRGCYC 1 cut(s) 841
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 6 cut(s) 278, 500, 685, 845, 899, 1102
FaqI GGGAC 4 cut(s) 54, 459, 563, 906
FatI CATG 6 cut(s) 274, 496, 681, 841, 895, 1098
FauI CCCGC 3 cut(s) 146, 160, 266
FbaI TGATCA 1 cut(s) 613
Fnu4HI GCNGC 7 cut(s) 135, 303, 564, 822, 864, 928, 1092
FokI GGATG 3 cut(s) 354, 483, 1178
FriOI GRGCYC 1 cut(s) 841
Fsp4HI GCNGC 7 cut(s) 135, 303, 564, 822, 864, 928, 1092
FspBI CTAG 3 cut(s) 651, 825, 1139
GlaI GCGC 1 cut(s) 1182
GluI GCNGC 7 cut(s) 135, 303, 564, 822, 864, 928, 1092
GsaI CCCAGC 1 cut(s) 704
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 1184
HaeIII GGCC 5 cut(s) 98, 257, 680, 750, 1025
HapII CCGG 1 cut(s) 767
HgaI GACGC 1 cut(s) 932
HhaI GCGC 1 cut(s) 1183
Hin1I GRCGYC 1 cut(s) 943
Hin1II CATG 6 cut(s) 278, 500, 685, 845, 899, 1102
Hin6I GCGC 1 cut(s) 1181
HinP1I GCGC 1 cut(s) 1181
HindIII AAGCTT 2 cut(s) 5, 966
HinfI GANTC 5 cut(s) 75, 342, 620, 693, 1244
HpaII CCGG 1 cut(s) 767
HphI GGTGA 2 cut(s) 24, 636
Hpy166II GTNNAC 2 cut(s) 241, 920
Hpy188I TCNGA 2 cut(s) 324, 675
Hpy188III TCNNGA 6 cut(s) 335, 359, 617, 767, 1016, 1151
Hpy8I GTNNAC 2 cut(s) 241, 920
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 4 cut(s) 68, 203, 319, 670
HpyCH4III ACNGT 1 cut(s) 982
HpyCH4V TGCA 7 cut(s) 11, 821, 866, 920, 1102, 1107, 1169
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 830, 1091
HpyF3I CTNAG 5 cut(s) 291, 400, 1037, 1053, 1272
Hsp92I GRCGYC 1 cut(s) 943
Hsp92II CATG 6 cut(s) 278, 500, 685, 845, 899, 1102
HspAI GCGC 1 cut(s) 1181
Kpn2I TCCGGA 1 cut(s) 766
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 4 cut(s) 453, 526, 613, 1201
LmnI GCTCC 3 cut(s) 220, 836, 1178
Lsp1109I GCAGC 6 cut(s) 289, 550, 833, 850, 939, 1078
LweI GCATC 4 cut(s) 285, 376, 1078, 1156
MaeI CTAG 3 cut(s) 651, 825, 1139
MaeIII GTNAC 5 cut(s) 30, 43, 395, 758, 948
MalI GATC 4 cut(s) 455, 528, 615, 1203
MbiI CCGCTC 1 cut(s) 167
MboI GATC 4 cut(s) 453, 526, 613, 1201
MboII GAAGA 9 cut(s) 93, 96, 99, 102, 105, 108, 111, 114, 117
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 3 cut(s) 514, 841, 922
MlsI TGGCCA 1 cut(s) 1025
MluCI AATT 6 cut(s) 264, 384, 457, 463, 600, 1146
MluNI TGGCCA 1 cut(s) 1025
MmeI TCCRAC 2 cut(s) 835, 1156
Mox20I TGGCCA 1 cut(s) 1025
Mph1103I ATGCAT 1 cut(s) 1104
MroI TCCGGA 1 cut(s) 766
MscI TGGCCA 1 cut(s) 1025
MseI TTAA 5 cut(s) 462, 591, 729, 773, 870
MslI CAYNNNNRTG 2 cut(s) 293, 1112
Msp20I TGGCCA 1 cut(s) 1025
MspA1I CMGCKG 2 cut(s) 704, 930
MspCI CTTAAG 1 cut(s) 772
MspI CCGG 1 cut(s) 767
MspR9I CCNGG 3 cut(s) 280, 410, 1059
MvaI CCWGG 3 cut(s) 280, 410, 1059
MwoI GCNNNNNNNGC 3 cut(s) 280, 830, 1091
NdeII GATC 4 cut(s) 453, 526, 613, 1201
NlaIII CATG 6 cut(s) 278, 500, 685, 845, 899, 1102
NlaIV GGNNCC 2 cut(s) 216, 528
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 2 cut(s) 30, 758
NsiI ATGCAT 1 cut(s) 1104
NspI RCATGY 2 cut(s) 278, 899
PaeI GCATGC 1 cut(s) 278
PceI AGGCCT 1 cut(s) 98
PciI ACATGT 1 cut(s) 895
PfeI GAWTC 5 cut(s) 75, 342, 620, 693, 1244
PflFI GACNNNGTC 1 cut(s) 1157
PflMI CCANNNNNTGG 1 cut(s) 1190
PkrI GCNGC 7 cut(s) 136, 304, 565, 823, 865, 929, 1093
PscI ACATGT 1 cut(s) 895
Psp124BI GAGCTC 1 cut(s) 841
Psp6I CCWGG 3 cut(s) 278, 408, 1057
PspFI CCCAGC 1 cut(s) 700
PspGI CCWGG 3 cut(s) 278, 408, 1057
PspN4I GGNNCC 2 cut(s) 216, 528
PspPI GGNCC 2 cut(s) 231, 256
PsrI GAACNNNNNNTAC 1 cut(s) 1245
PsuI RGATCY 1 cut(s) 526
PsyI GACNNNGTC 1 cut(s) 1157
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 2 cut(s) 350, 740
RsaNI GTAC 2 cut(s) 349, 739
RseI CAYNNNNRTG 2 cut(s) 293, 1112
SacI GAGCTC 1 cut(s) 841
SaqAI TTAA 5 cut(s) 462, 591, 729, 773, 870
SatI GCNGC 7 cut(s) 135, 303, 564, 822, 864, 928, 1092
Sau3AI GATC 4 cut(s) 453, 526, 613, 1201
Sau96I GGNCC 2 cut(s) 231, 256
ScaI AGTACT 1 cut(s) 740
ScrFI CCNGG 3 cut(s) 280, 410, 1059
SduI GDGCHC 3 cut(s) 514, 841, 922
SfaNI GCATC 4 cut(s) 285, 376, 1078, 1156
SfcI CTRYAG 1 cut(s) 244
SinI GGWCC 1 cut(s) 231
SmiMI CAYNNNNRTG 2 cut(s) 293, 1112
SmlI CTYRAG 2 cut(s) 772, 953
SmoI CTYRAG 2 cut(s) 772, 953
SpeI ACTAGT 1 cut(s) 1138
SphI GCATGC 1 cut(s) 278
Sse9I AATT 6 cut(s) 264, 384, 457, 463, 600, 1146
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 7 cut(s) 132, 135, 139, 153, 165, 259, 930
SspI AATATT 1 cut(s) 436
SspMI CTAG 3 cut(s) 651, 825, 1139
SstI GAGCTC 1 cut(s) 841
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 278, 408, 1057
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 982
TaqI TCGA 3 cut(s) 598, 712, 1015
TaqII GACCGA 1 cut(s) 248
TasI AATT 6 cut(s) 264, 384, 457, 463, 600, 1146
TatI WGTACW 2 cut(s) 348, 738
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 5 cut(s) 75, 342, 620, 693, 1244
Tru1I TTAA 5 cut(s) 462, 591, 729, 773, 870
Tru9I TTAA 5 cut(s) 462, 591, 729, 773, 870
TscAI CASTG 3 cut(s) 214, 295, 561
TseFI GTSAC 2 cut(s) 30, 758
TseI GCWGC 6 cut(s) 302, 563, 821, 863, 927, 1091
Tsp45I GTSAC 2 cut(s) 30, 758
TspDTI ATGAA 6 cut(s) 9, 17, 612, 649, 1087, 1130
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 3 cut(s) 214, 295, 561
Tth111I GACNNNGTC 1 cut(s) 1157
Van91I CCANNNNNTGG 1 cut(s) 1190
Vha464I CTTAAG 1 cut(s) 772
VneI GTGCAC 1 cut(s) 918
VpaK11BI GGWCC 1 cut(s) 231
XceI RCATGY 2 cut(s) 278, 899
XspI CTAG 3 cut(s) 651, 825, 1139
ZrmI AGTACT 1 cut(s) 740
Zsp2I ATGCAT 1 cut(s) 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.