RLG00000030665

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008425
Physical Location & Seq
Forward (+)
65403722 .. 65408550
4829 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030665

Sequence Viewer

Length: 1278 bp
ATGAAGCTTGCAACTTGTTCATATTCGTCGTCACCAACCTCCGTTACTCTTGCCCGTCCCAGATTCTCATTCAATATATTTCAGCTCTCTTCTTCTTCTTCTTCTTCAAGCGCCGCCCACCGACCACGACCCGTCATGGTTTCCGCTCGCAACTACGCCCACGCCCACTACCTTCCTCCTTGGTTCAGTGTGGCTCCTATGATGGAATGGACTGATGTTCACTACAGGACTATGGCCCGCCTAATTTCCAAGCATGCCTGGCTCTACACTGAGATGCTCGCTGCCGAAACCATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCAGAACAGCATCCTATTGTTCTACAAATTGGTGGCAGTAACTTAGAAAACCTGGCAAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTCGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCCGAGGCTATGTCAGTGATTGGTGCGAACACAGATGTCCCTGTCAGCGTTAAGTGTCGAATTGGTGTTGATGATCACGATTCATATAATGAGCTCTGTGATTTTATTTATAAAGTTTCTTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCCATGCTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTTAAATACGAGTACTTTTATGGCCTCTTGCGTGACTTTCCGGACTTAAGATTTACCATAAATGGTGGAATAAACACTATTGATGAGGTCAATGCAGCTAGGAAAGCAGGAGCTCATGGTGTAATGGTTGGACGGGCTGCATTTAATAACTCATGGAATACTTTGGGACATGTTGATACTGCTATTTATGGTGCACCAAGCAGCGGTATAACACGGCGTCAGTTACTCAAGGAGTTTGAAGCTTATGGAGAGACTGTTTTGGGAAAATATGGACAAAAGCCCAATGTTCGAGATGTGGCCAAGCCTTTGCTTAGCCTTTTCTATGCTCAGCCTGGGAACAGCCTTTGGAAACGCAAAGCTGATGCTGCTTTCATGCATTGCACGACTATGAAATCATTTTTTGAGGAAACACTTGTAGCAATTCCAGACTTTGTCTTGGATGCACCTGTTGGAGCGCCACCATCTGGTAGTAATGATCCTTTTTCAAACATACATACTTTGTTGCCTCCACCATACGAATCAAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

426

Amino Acids

47.38

Weight (kDa)

8.35

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 64 - 371 4.9e-57 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
AccB7I CCANNNNNTGG 1 cut(s) 1193
AccBSI CCGCTC 1 cut(s) 146
AccIII TCCGGA 1 cut(s) 769
AciI CCGC 4 cut(s) 114, 144, 238, 933
AclWI GGATC 3 cut(s) 500, 513, 1199
AcoI YGGCCR 1 cut(s) 1026
AcyI GRCGYC 1 cut(s) 946
AfaI GTAC 2 cut(s) 329, 743
AfiI CCNNNNNNNGG 2 cut(s) 932, 1193
AflII CTTAAG 1 cut(s) 775
AflIII ACRYGT 1 cut(s) 898
AgsI TTSAA 4 cut(s) 73, 108, 968, 1215
AjnI CCWGG 3 cut(s) 257, 387, 1060
Alw21I GWGCWC 4 cut(s) 493, 615, 844, 925
Alw26I GTCTC 1 cut(s) 974
Alw44I GTGCAC 1 cut(s) 921
AlwI GGATC 3 cut(s) 500, 513, 1199
Aor13HI TCCGGA 1 cut(s) 769
AoxI GGCC 4 cut(s) 234, 681, 751, 1026
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 5 cut(s) 281, 824, 866, 930, 1094
ArsI GACNNNNNNTTYG 2 cut(s) 279, 311
AspLEI GCGC 2 cut(s) 113, 1186
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 24, 639
BaeGI GKGCMC 1 cut(s) 925
BalI TGGCCA 1 cut(s) 1028
BamHI GGATCC 1 cut(s) 505
BanII GRGCYC 2 cut(s) 615, 844
Bbv12I GWGCWC 4 cut(s) 493, 615, 844, 925
BbvI GCAGC 5 cut(s) 268, 836, 853, 942, 1081
BccI CCATC 2 cut(s) 196, 1198
BceAI ACGGC 1 cut(s) 959
BciT130I CCWGG 3 cut(s) 259, 389, 1062
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 1 cut(s) 974
BfaI CTAG 2 cut(s) 654, 828
BfmI CTRYAG 1 cut(s) 223
BfoI RGCGCY 2 cut(s) 114, 1187
BfrI CTTAAG 1 cut(s) 775
BisI GCNGC 6 cut(s) 114, 282, 825, 867, 931, 1095
BlpI GCTNAGC 2 cut(s) 1040, 1056
BlsI GCNGC 6 cut(s) 115, 283, 826, 868, 932, 1096
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 3 cut(s) 259, 389, 1062
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 2 cut(s) 195, 507
BmrFI CCNGG 3 cut(s) 259, 389, 1062
BmrI ACTGGG 1 cut(s) 450
BmsI GCATC 4 cut(s) 264, 355, 1081, 1159
BmuI ACTGGG 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 321
Bpu1102I GCTNAGC 2 cut(s) 1040, 1056
BpuEI CTTGAG 1 cut(s) 941
BsaHI GRCGYC 1 cut(s) 946
BsaJI CCNNGG 3 cut(s) 179, 522, 1061
BsaWI WCCGGW 1 cut(s) 769
BsaXI ACNNNNNCTCC 2 cut(s) 969, 999
Bsc4I CCNNNNNNNGG 2 cut(s) 932, 1193
Bse1I ACTGG 1 cut(s) 456
Bse3DI GCAATG 1 cut(s) 1105
BseAI TCCGGA 1 cut(s) 769
BseBI CCWGG 3 cut(s) 259, 389, 1062
BseDI CCNNGG 3 cut(s) 179, 522, 1061
BseGI GGATG 3 cut(s) 346, 455, 1174
BseLI CCNNNNNNNGG 2 cut(s) 932, 1193
BseMI GCAATG 1 cut(s) 1105
BseMII CTCAG 2 cut(s) 261, 1070
BseNI ACTGG 1 cut(s) 456
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 5 cut(s) 268, 836, 853, 942, 1081
BseYI CCCAGC 1 cut(s) 703
BshFI GGCC 4 cut(s) 236, 683, 753, 1028
BsiHKAI GWGCWC 4 cut(s) 493, 615, 844, 925
BsiSI CCGG 1 cut(s) 770
BslFI GGGAC 4 cut(s) 42, 438, 542, 909
BslI CCNNNNNNNGG 2 cut(s) 932, 1193
BsmAI GTCTC 1 cut(s) 974
BsmFI GGGAC 4 cut(s) 42, 438, 542, 909
BsnI GGCC 4 cut(s) 236, 683, 753, 1028
Bsp1286I GDGCHC 4 cut(s) 493, 615, 844, 925
Bsp13I TCCGGA 1 cut(s) 769
Bsp143I GATC 4 cut(s) 432, 505, 592, 1204
Bsp1720I GCTNAGC 2 cut(s) 1040, 1056
BspACI CCGC 4 cut(s) 114, 144, 238, 933
BspANI GGCC 4 cut(s) 236, 683, 753, 1028
BspCNI CTCAG 2 cut(s) 262, 1069
BspEI TCCGGA 1 cut(s) 769
BspLI GGNNCC 2 cut(s) 195, 507
BspPI GGATC 3 cut(s) 500, 513, 1199
BspTI CTTAAG 1 cut(s) 775
BsrBI CCGCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 1105
BsrI ACTGG 1 cut(s) 456
BssECI CCNNGG 3 cut(s) 179, 522, 1061
BssMI GATC 4 cut(s) 432, 505, 592, 1204
BssNI GRCGYC 1 cut(s) 946
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 3 cut(s) 259, 389, 1062
Bst4CI ACNGT 1 cut(s) 985
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 1 cut(s) 946
BstAFI CTTAAG 1 cut(s) 775
BstC8I GCNNGC 5 cut(s) 9, 148, 238, 255, 279
BstDEI CTNAG 5 cut(s) 270, 379, 1040, 1056, 1275
BstF5I GGATG 3 cut(s) 346, 455, 1174
BstH2I RGCGCY 2 cut(s) 114, 1187
BstHHI GCGC 2 cut(s) 113, 1186
BstKTI GATC 4 cut(s) 435, 508, 595, 1207
BstMAI GTCTC 1 cut(s) 974
BstMBI GATC 4 cut(s) 432, 505, 592, 1204
BstMWI GCNNNNNNNGC 3 cut(s) 259, 833, 1094
BstNI CCWGG 3 cut(s) 259, 389, 1062
BstNSI RCATGY 2 cut(s) 257, 902
BstSCI CCNGG 3 cut(s) 257, 387, 1060
BstSFI CTRYAG 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 925
BstV1I GCAGC 5 cut(s) 268, 836, 853, 942, 1081
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BsuRI GGCC 4 cut(s) 236, 683, 753, 1028
BtsCI GGATG 3 cut(s) 346, 455, 1174
BtsIMutI CAGTG 3 cut(s) 193, 267, 540
Cac8I GCNNGC 5 cut(s) 9, 148, 238, 255, 279
CfoI GCGC 2 cut(s) 113, 1186
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 1 cut(s) 935
Csp6I GTAC 2 cut(s) 328, 742
CviAII CATG 8 cut(s) 136, 254, 476, 685, 845, 882, 899, 1102
CviQI GTAC 2 cut(s) 328, 742
DdeI CTNAG 5 cut(s) 270, 379, 1040, 1056, 1275
DpnI GATC 4 cut(s) 434, 507, 594, 1206
DpnII GATC 4 cut(s) 432, 505, 592, 1204
EaeI YGGCCR 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Ecl136II GAGCTC 2 cut(s) 613, 842
Eco130I CCWWGG 1 cut(s) 179
Eco24I GRGCYC 2 cut(s) 615, 844
Eco53kI GAGCTC 2 cut(s) 613, 842
EcoICRI GAGCTC 2 cut(s) 613, 842
EcoRII CCWGG 3 cut(s) 257, 387, 1060
EcoT14I CCWWGG 1 cut(s) 179
EcoT22I ATGCAT 1 cut(s) 1107
EcoT38I GRGCYC 2 cut(s) 615, 844
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 8 cut(s) 139, 257, 479, 688, 848, 885, 902, 1105
FaqI GGGAC 4 cut(s) 42, 438, 542, 909
FatI CATG 8 cut(s) 135, 253, 475, 684, 844, 881, 898, 1101
FauI CCCGC 1 cut(s) 245
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 6 cut(s) 114, 282, 825, 867, 931, 1095
FokI GGATG 3 cut(s) 333, 462, 1181
FriOI GRGCYC 2 cut(s) 615, 844
Fsp4HI GCNGC 6 cut(s) 114, 282, 825, 867, 931, 1095
FspBI CTAG 2 cut(s) 654, 828
GlaI GCGC 2 cut(s) 112, 1185
GluI GCNGC 6 cut(s) 114, 282, 825, 867, 931, 1095
GsaI CCCAGC 1 cut(s) 707
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 2 cut(s) 114, 1187
HaeIII GGCC 4 cut(s) 236, 683, 753, 1028
HapII CCGG 1 cut(s) 770
HgaI GACGC 1 cut(s) 935
HhaI GCGC 2 cut(s) 113, 1186
Hin1I GRCGYC 1 cut(s) 946
Hin1II CATG 8 cut(s) 139, 257, 479, 688, 848, 885, 902, 1105
Hin6I GCGC 2 cut(s) 111, 1184
HinP1I GCGC 2 cut(s) 111, 1184
HindIII AAGCTT 2 cut(s) 5, 969
HinfI GANTC 5 cut(s) 63, 321, 599, 696, 1247
HpaII CCGG 1 cut(s) 770
HphI GGTGA 2 cut(s) 24, 639
Hpy166II GTNNAC 2 cut(s) 220, 923
Hpy188I TCNGA 2 cut(s) 303, 678
Hpy188III TCNNGA 6 cut(s) 314, 338, 596, 770, 1019, 1154
Hpy8I GTNNAC 2 cut(s) 220, 923
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 3 cut(s) 182, 298, 673
HpyCH4III ACNGT 1 cut(s) 985
HpyCH4V TGCA 7 cut(s) 11, 824, 869, 923, 1105, 1110, 1172
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 833, 1094
HpyF3I CTNAG 5 cut(s) 270, 379, 1040, 1056, 1275
Hsp92I GRCGYC 1 cut(s) 946
Hsp92II CATG 8 cut(s) 139, 257, 479, 688, 848, 885, 902, 1105
HspAI GCGC 2 cut(s) 111, 1184
Kpn2I TCCGGA 1 cut(s) 769
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 4 cut(s) 432, 505, 592, 1204
LmnI GCTCC 3 cut(s) 199, 839, 1181
Lsp1109I GCAGC 5 cut(s) 268, 836, 853, 942, 1081
LweI GCATC 4 cut(s) 264, 355, 1081, 1159
MaeI CTAG 2 cut(s) 654, 828
MaeIII GTNAC 5 cut(s) 30, 43, 374, 761, 951
MalI GATC 4 cut(s) 434, 507, 594, 1206
MbiI CCGCTC 1 cut(s) 146
MboI GATC 4 cut(s) 432, 505, 592, 1204
MboII GAAGA 7 cut(s) 81, 84, 87, 90, 93, 96, 630
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 4 cut(s) 493, 615, 844, 925
MlsI TGGCCA 1 cut(s) 1028
MluCI AATT 6 cut(s) 243, 363, 436, 442, 579, 1149
MluNI TGGCCA 1 cut(s) 1028
MmeI TCCRAC 2 cut(s) 838, 1159
MnlI CCTC 9 cut(s) 49, 186, 345, 517, 738, 764, 808, 1126, 1245
Mox20I TGGCCA 1 cut(s) 1028
Mph1103I ATGCAT 1 cut(s) 1107
MroI TCCGGA 1 cut(s) 769
MscI TGGCCA 1 cut(s) 1028
MseI TTAA 5 cut(s) 441, 570, 732, 776, 873
MslI CAYNNNNRTG 2 cut(s) 272, 1115
Msp20I TGGCCA 1 cut(s) 1028
MspA1I CMGCKG 2 cut(s) 707, 933
MspCI CTTAAG 1 cut(s) 775
MspI CCGG 1 cut(s) 770
MspR9I CCNGG 3 cut(s) 259, 389, 1062
MvaI CCWGG 3 cut(s) 259, 389, 1062
MwoI GCNNNNNNNGC 3 cut(s) 259, 833, 1094
NdeII GATC 4 cut(s) 432, 505, 592, 1204
NlaIII CATG 8 cut(s) 139, 257, 479, 688, 848, 885, 902, 1105
NlaIV GGNNCC 2 cut(s) 195, 507
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 30, 761
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 2 cut(s) 257, 902
PaeI GCATGC 1 cut(s) 257
PciI ACATGT 1 cut(s) 898
PfeI GAWTC 5 cut(s) 63, 321, 599, 696, 1247
PflFI GACNNNGTC 1 cut(s) 1160
PflMI CCANNNNNTGG 1 cut(s) 1193
PkrI GCNGC 6 cut(s) 115, 283, 826, 868, 932, 1096
PscI ACATGT 1 cut(s) 898
PsiI TTATAA 1 cut(s) 630
Psp124BI GAGCTC 2 cut(s) 615, 844
Psp6I CCWGG 3 cut(s) 257, 387, 1060
PspFI CCCAGC 1 cut(s) 703
PspGI CCWGG 3 cut(s) 257, 387, 1060
PspN4I GGNNCC 2 cut(s) 195, 507
PspPI GGNCC 1 cut(s) 235
PsrI GAACNNNNNNTAC 1 cut(s) 1248
PsuI RGATCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 1160
PvuII CAGCTG 1 cut(s) 707
RsaI GTAC 2 cut(s) 329, 743
RsaNI GTAC 2 cut(s) 328, 742
RseI CAYNNNNRTG 2 cut(s) 272, 1115
SacI GAGCTC 2 cut(s) 615, 844
SaqAI TTAA 5 cut(s) 441, 570, 732, 776, 873
SatI GCNGC 6 cut(s) 114, 282, 825, 867, 931, 1095
Sau3AI GATC 4 cut(s) 432, 505, 592, 1204
Sau96I GGNCC 1 cut(s) 235
ScaI AGTACT 1 cut(s) 743
ScrFI CCNGG 3 cut(s) 259, 389, 1062
SduI GDGCHC 4 cut(s) 493, 615, 844, 925
SfaNI GCATC 4 cut(s) 264, 355, 1081, 1159
SfcI CTRYAG 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 272, 1115
SmlI CTYRAG 2 cut(s) 775, 956
SmoI CTYRAG 2 cut(s) 775, 956
SphI GCATGC 1 cut(s) 257
Sse9I AATT 6 cut(s) 243, 363, 436, 442, 579, 1149
SsiI CCGC 4 cut(s) 114, 144, 238, 933
SspI AATATT 1 cut(s) 415
SspMI CTAG 2 cut(s) 654, 828
SstI GAGCTC 2 cut(s) 615, 844
StyD4I CCNGG 3 cut(s) 257, 387, 1060
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 985
TaqI TCGA 3 cut(s) 577, 715, 1018
TasI AATT 6 cut(s) 243, 363, 436, 442, 579, 1149
TatI WGTACW 2 cut(s) 327, 741
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 5 cut(s) 63, 321, 599, 696, 1247
Tru1I TTAA 5 cut(s) 441, 570, 732, 776, 873
Tru9I TTAA 5 cut(s) 441, 570, 732, 776, 873
TscAI CASTG 3 cut(s) 193, 274, 540
TseFI GTSAC 2 cut(s) 30, 761
TseI GCWGC 5 cut(s) 281, 824, 866, 930, 1094
Tsp45I GTSAC 2 cut(s) 30, 761
TspDTI ATGAA 6 cut(s) 9, 17, 591, 652, 1090, 1133
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 3 cut(s) 193, 274, 540
Tth111I GACNNNGTC 1 cut(s) 1160
Van91I CCANNNNNTGG 1 cut(s) 1193
Vha464I CTTAAG 1 cut(s) 775
VneI GTGCAC 1 cut(s) 921
XceI RCATGY 2 cut(s) 257, 902
XspI CTAG 2 cut(s) 654, 828
ZrmI AGTACT 1 cut(s) 743
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.