MD07G1012900.v1.1

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008425
Physical Location & Seq
Reverse (-)
1141188 .. 1147512
6325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1012900.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGTTTCTTCTCTCGCCACCCACCCTCACCACGCCCATTACCCTCCTCCTTGGTTTAGTATTGCTCCCATGATGGAGTGGACTGACAATCACTACAGGACGCTGGCCAGGCTCATTTCGAAGAATGCGTGGCTCTACACCGAGATGCTTGCTGCTGAGACCATTGTCTACCAGAAGGATAATCTGGATAGATTCTTGGAATATTCCCCGGAACAACATCCTATTGTTCTTCAAATTGGCGGGAATAATTTGGAAAACCTCGCCAAAGCGACTGAGCTTGCTAACCCTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCGAGTCCAAGAGTAGCTGGGCACGGGTGCTTTGGTGCTAGTCTTATGCTTGATCCAAAGTTTGTTGCTGAGGCTATGTCAGTGATTGCTGCGCACACAGATGCACCTGTTAGCGTGAAATGTCGAATTGGTGTTGATAATCATGACTCATATAACGAGCTCTGTGATTTTATTTACAAGGTTTCTTCTCAATCACCAACTAGGCATTTCATAATACATTCACGGAAGGCACTACTTAATGGAATTAGCCCAGCTGAAAATCGAAGTATTCCGCCTCTTAAATATGAGTACTTTTATGGCCTCTTGCGTGACTTTCCAGACTTGAGATTTACAATAAATGGGGGCATAAACACCGTTGAGGAGGTCAATGCAGCAAGAAGGGCAGGAGCTCATGGTGTAATGGTTGGACGCGCTGCATTTAATAAACCATGGCATACTTTGGGACATGTTGATACTGTAGTTTATGGAGCACCAAGCAGTGGTGTAACACGGCGTCAGATACTGGAGAAGTTTCGAGCGTATGGAGATACTGCTGTGGGAAAATATGGACGCAAACCAACTGTGCGAGATGTAGCAAGGCCTTTGATTGGTCTTTTCCATTCAGAACCTGGGAATAGTCAATGGAAACGCAAGGCTGATGCTGCTTTCCTGCATTGCACGACCATGAAAGAGTTTTTTGATGAAACGCTTGTAGCGATTCCTGACTATGTTTTGGATAAACCTATTGGGGAGCCACCATCAGGCAGTGAAGATCCTTTTGCGAACATACACAGTTTGCTGCCTCCGGCGTATGAATCAAGGGAACTAGAACTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

383

Amino Acids

42.77

Weight (kDa)

6.49

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 21 - 329 7e-60 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 803
AccI GTMKAC 1 cut(s) 168
AccII CGCG 1 cut(s) 735
AciI CCGC 2 cut(s) 240, 596
AclWI GGATC 2 cut(s) 371, 1070
AcoI YGGCCR 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 817
AfaI GTAC 1 cut(s) 614
AfiI CCNNNNNNNGG 3 cut(s) 803, 911, 1064
AflIII ACRYGT 1 cut(s) 769
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 2 cut(s) 107, 931
AluBI AGCT 5 cut(s) 277, 341, 484, 578, 713
AluI AGCT 5 cut(s) 277, 341, 484, 578, 713
Alw21I GWGCWC 3 cut(s) 486, 715, 796
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 2 cut(s) 371, 1070
AlwNI CAGNNNCTG 2 cut(s) 826, 932
AoxI GGCC 3 cut(s) 105, 622, 902
ApeKI GCWGC 6 cut(s) 152, 413, 695, 737, 965, 1102
AspLEI GCGC 2 cut(s) 418, 737
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 2 cut(s) 20, 510
AsuII TTCGAA 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 348
BaeI ACNNNNGTAYC 4 cut(s) 768, 801, 843, 876
BalI TGGCCA 1 cut(s) 107
BanII GRGCYC 2 cut(s) 486, 715
Bbv12I GWGCWC 3 cut(s) 486, 715, 796
BbvCI CCTCAGC 1 cut(s) 393
BbvI GCAGC 6 cut(s) 139, 400, 707, 724, 952, 1089
BccI CCATC 2 cut(s) 67, 1069
BceAI ACGGC 1 cut(s) 830
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 2 cut(s) 109, 933
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 3 cut(s) 363, 525, 1130
BfmI CTRYAG 2 cut(s) 94, 780
BisI GCNGC 6 cut(s) 153, 414, 696, 738, 966, 1103
BlsI GCNGC 6 cut(s) 154, 415, 697, 739, 967, 1104
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 3 cut(s) 109, 209, 933
BmiI GGNNCC 1 cut(s) 1056
BmrFI CCNGG 3 cut(s) 109, 209, 933
BmsI GCATC 3 cut(s) 135, 415, 952
BoxI GACNNNNGTC 1 cut(s) 164
BpmI CTGGAG 1 cut(s) 848
Bpu10I CCTNAGC 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 667
BpuMI CCSGG 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 817
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 4 cut(s) 50, 207, 752, 932
Bsc4I CCNNNNNNNGG 3 cut(s) 803, 911, 1064
Bse1I ACTGG 1 cut(s) 831
Bse3DI GCAATG 1 cut(s) 976
BseBI CCWGG 2 cut(s) 109, 933
BseDI CCNNGG 4 cut(s) 50, 207, 752, 932
BseGI GGATG 2 cut(s) 217, 326
BseLI CCNNNNNNNGG 3 cut(s) 803, 911, 1064
BseMI GCAATG 1 cut(s) 976
BseMII CTCAG 3 cut(s) 147, 264, 384
BseNI ACTGG 1 cut(s) 831
BseRI GAGGAG 2 cut(s) 36, 698
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 6 cut(s) 139, 400, 707, 724, 952, 1089
BseYI CCCAGC 2 cut(s) 341, 574
Bsh1236I CGCG 1 cut(s) 735
BshFI GGCC 3 cut(s) 107, 624, 904
BsiHKAI GWGCWC 3 cut(s) 486, 715, 796
BsiSI CCGG 2 cut(s) 209, 1109
BslFI GGGAC 1 cut(s) 780
BslI CCNNNNNNNGG 3 cut(s) 803, 911, 1064
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 780
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 3 cut(s) 107, 624, 904
Bso31I GGTCTC 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 4 cut(s) 348, 486, 715, 796
Bsp143I GATC 3 cut(s) 303, 376, 1075
Bsp19I CCATGG 1 cut(s) 752
BspACI CCGC 2 cut(s) 240, 596
BspANI GGCC 3 cut(s) 107, 624, 904
BspCNI CTCAG 3 cut(s) 148, 265, 385
BspFNI CGCG 1 cut(s) 735
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 1056
BspPI GGATC 2 cut(s) 371, 1070
BspT104I TTCGAA 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 976
BsrI ACTGG 1 cut(s) 831
BssECI CCNNGG 4 cut(s) 50, 207, 752, 932
BssMI GATC 3 cut(s) 303, 376, 1075
BssNI GRCGYC 1 cut(s) 817
BssT1I CCWWGG 2 cut(s) 50, 752
Bst2UI CCWGG 2 cut(s) 109, 933
Bst4CI ACNGT 4 cut(s) 679, 781, 886, 1097
BstACI GRCGYC 1 cut(s) 817
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 3 cut(s) 105, 150, 279
BstDEI CTNAG 4 cut(s) 156, 273, 393, 1146
BstDSI CCRYGG 1 cut(s) 752
BstF5I GGATG 2 cut(s) 217, 326
BstFNI CGCG 1 cut(s) 735
BstHHI GCGC 2 cut(s) 418, 737
BstKTI GATC 3 cut(s) 306, 379, 1078
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 303, 376, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 109, 704, 965
BstNI CCWGG 2 cut(s) 109, 933
BstNSI RCATGY 1 cut(s) 773
BstPAI GACNNNNGTC 1 cut(s) 164
BstSCI CCNGG 3 cut(s) 107, 207, 931
BstSFI CTRYAG 2 cut(s) 94, 780
BstSLI GKGCMC 1 cut(s) 348
BstUI CGCG 1 cut(s) 735
BstV1I GCAGC 6 cut(s) 139, 400, 707, 724, 952, 1089
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BsuRI GGCC 3 cut(s) 107, 624, 904
BtgI CCRYGG 1 cut(s) 752
BtsCI GGATG 2 cut(s) 217, 326
BtsI GCAGTG 2 cut(s) 808, 1075
BtsIMutI CAGTG 3 cut(s) 411, 808, 1075
Cac8I GCNNGC 3 cut(s) 105, 150, 279
CaiI CAGNNNCTG 2 cut(s) 826, 932
CciI TCATGA 1 cut(s) 466
CfoI GCGC 2 cut(s) 418, 737
CseI GACGC 4 cut(s) 109, 741, 806, 882
Csp6I GTAC 1 cut(s) 613
CviAII CATG 6 cut(s) 70, 467, 716, 753, 770, 988
CviQI GTAC 1 cut(s) 613
DdeI CTNAG 4 cut(s) 156, 273, 393, 1146
DpnI GATC 3 cut(s) 305, 378, 1077
DpnII GATC 3 cut(s) 303, 376, 1075
EaeI YGGCCR 1 cut(s) 105
EciI GGCGGA 1 cut(s) 585
Ecl136II GAGCTC 2 cut(s) 484, 713
Eco130I CCWWGG 2 cut(s) 50, 752
Eco147I AGGCCT 1 cut(s) 904
Eco24I GRGCYC 2 cut(s) 486, 715
Eco31I GGTCTC 1 cut(s) 152
Eco53kI GAGCTC 2 cut(s) 484, 713
EcoICRI GAGCTC 2 cut(s) 484, 713
EcoRII CCWGG 2 cut(s) 107, 931
EcoT14I CCWWGG 2 cut(s) 50, 752
EcoT38I GRGCYC 2 cut(s) 486, 715
ErhI CCWWGG 2 cut(s) 50, 752
FaeI CATG 6 cut(s) 73, 470, 719, 756, 773, 991
FaqI GGGAC 1 cut(s) 780
FatI CATG 6 cut(s) 69, 466, 715, 752, 769, 987
FauI CCCGC 1 cut(s) 233
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 6 cut(s) 153, 414, 696, 738, 966, 1103
FokI GGATG 2 cut(s) 204, 333
FriOI GRGCYC 2 cut(s) 486, 715
Fsp4HI GCNGC 6 cut(s) 153, 414, 696, 738, 966, 1103
FspBI CTAG 3 cut(s) 363, 525, 1130
FspI TGCGCA 1 cut(s) 417
GlaI GCGC 2 cut(s) 417, 736
GluI GCNGC 6 cut(s) 153, 414, 696, 738, 966, 1103
GsaI CCCAGC 2 cut(s) 345, 578
GsuI CTGGAG 1 cut(s) 848
HaeIII GGCC 3 cut(s) 107, 624, 904
HapII CCGG 2 cut(s) 209, 1109
HgaI GACGC 4 cut(s) 109, 741, 806, 882
HhaI GCGC 2 cut(s) 418, 737
Hin1I GRCGYC 1 cut(s) 817
Hin1II CATG 6 cut(s) 73, 470, 719, 756, 773, 991
Hin6I GCGC 2 cut(s) 416, 735
HinP1I GCGC 2 cut(s) 416, 735
HinfI GANTC 5 cut(s) 192, 328, 470, 1021, 1118
HpaII CCGG 2 cut(s) 209, 1109
HphI GGTGA 2 cut(s) 20, 510
Hpy166II GTNNAC 2 cut(s) 81, 169
Hpy188I TCNGA 3 cut(s) 327, 822, 928
Hpy188III TCNNGA 4 cut(s) 185, 467, 641, 1025
Hpy8I GTNNAC 2 cut(s) 81, 169
HpyAV CCTTC 3 cut(s) 169, 544, 696
HpyCH4III ACNGT 4 cut(s) 679, 781, 886, 1097
HpyCH4V TGCA 5 cut(s) 428, 695, 740, 976, 981
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 704, 965
HpyF3I CTNAG 4 cut(s) 156, 273, 393, 1146
Hsp92I GRCGYC 1 cut(s) 817
Hsp92II CATG 6 cut(s) 73, 470, 719, 756, 773, 991
HspAI GCGC 2 cut(s) 416, 735
Kzo9I GATC 3 cut(s) 303, 376, 1075
LmnI GCTCC 4 cut(s) 70, 710, 791, 1054
Lsp1109I GCAGC 6 cut(s) 139, 400, 707, 724, 952, 1089
LweI GCATC 3 cut(s) 135, 415, 952
MaeI CTAG 3 cut(s) 363, 525, 1130
MaeIII GTNAC 2 cut(s) 632, 808
MalI GATC 3 cut(s) 305, 378, 1077
MboI GATC 3 cut(s) 303, 376, 1075
MboII GAAGA 4 cut(s) 133, 221, 501, 1085
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 4 cut(s) 348, 486, 715, 796
MlsI TGGCCA 1 cut(s) 107
MluCI AATT 6 cut(s) 234, 247, 307, 313, 450, 567
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 2 cut(s) 337, 464
MmeI TCCRAC 1 cut(s) 709
Mox20I TGGCCA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 107
MseI TTAA 4 cut(s) 312, 561, 603, 744
MslI CAYNNNNRTG 3 cut(s) 143, 423, 986
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 2 cut(s) 209, 1109
MspR9I CCNGG 3 cut(s) 109, 209, 933
Mva1269I GAATGC 1 cut(s) 130
MvaI CCWGG 2 cut(s) 109, 933
MvnI CGCG 1 cut(s) 735
MwoI GCNNNNNNNGC 3 cut(s) 109, 704, 965
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 752
NdeII GATC 3 cut(s) 303, 376, 1075
NlaIII CATG 6 cut(s) 73, 470, 719, 756, 773, 991
NlaIV GGNNCC 1 cut(s) 1056
NmuCI GTSAC 1 cut(s) 632
NsbI TGCGCA 1 cut(s) 417
NspI RCATGY 1 cut(s) 773
NspV TTCGAA 1 cut(s) 119
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 904
PciI ACATGT 1 cut(s) 769
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 1016
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 3 cut(s) 192, 1021, 1118
PflMI CCANNNNNTGG 1 cut(s) 803
PkrI GCNGC 6 cut(s) 154, 415, 697, 739, 967, 1104
PleI GAGTC 2 cut(s) 336, 464
PpsI GAGTC 2 cut(s) 336, 464
PscI ACATGT 1 cut(s) 769
PshAI GACNNNNGTC 1 cut(s) 164
Psp124BI GAGCTC 2 cut(s) 486, 715
Psp6I CCWGG 2 cut(s) 107, 931
PspFI CCCAGC 2 cut(s) 341, 574
PspGI CCWGG 2 cut(s) 107, 931
PspN4I GGNNCC 1 cut(s) 1056
PsrI GAACNNNNNNTAC 1 cut(s) 1119
PstNI CAGNNNCTG 2 cut(s) 826, 932
PsuI RGATCY 1 cut(s) 1075
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
RseI CAYNNNNRTG 3 cut(s) 143, 423, 986
SacI GAGCTC 2 cut(s) 486, 715
SaqAI TTAA 4 cut(s) 312, 561, 603, 744
SatI GCNGC 6 cut(s) 153, 414, 696, 738, 966, 1103
Sau3AI GATC 3 cut(s) 303, 376, 1075
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 2 cut(s) 337, 464
ScrFI CCNGG 3 cut(s) 109, 209, 933
SduI GDGCHC 4 cut(s) 348, 486, 715, 796
SfaNI GCATC 3 cut(s) 135, 415, 952
SfcI CTRYAG 2 cut(s) 94, 780
SfuI TTCGAA 1 cut(s) 119
SmiMI CAYNNNNRTG 3 cut(s) 143, 423, 986
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
Sse9I AATT 6 cut(s) 234, 247, 307, 313, 450, 567
SseBI AGGCCT 1 cut(s) 904
SsiI CCGC 2 cut(s) 240, 596
SspI AATATT 1 cut(s) 203
SspMI CTAG 3 cut(s) 363, 525, 1130
SstI GAGCTC 2 cut(s) 486, 715
StuI AGGCCT 1 cut(s) 904
StyD4I CCNGG 3 cut(s) 107, 207, 931
StyI CCWWGG 2 cut(s) 50, 752
TaaI ACNGT 4 cut(s) 679, 781, 886, 1097
TaqI TCGA 4 cut(s) 119, 448, 586, 838
TasI AATT 6 cut(s) 234, 247, 307, 313, 450, 567
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 3 cut(s) 192, 1021, 1118
Tru1I TTAA 4 cut(s) 312, 561, 603, 744
Tru9I TTAA 4 cut(s) 312, 561, 603, 744
TscAI CASTG 3 cut(s) 411, 808, 1075
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 6 cut(s) 152, 413, 695, 737, 965, 1102
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 4 cut(s) 523, 1004, 1020, 1131
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 3 cut(s) 411, 808, 1075
Van91I CCANNNNNTGG 1 cut(s) 803
XceI RCATGY 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 929
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 3 cut(s) 363, 525, 1130
ZrmI AGTACT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.