Rroxscaffold_4G00330940

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008425
Physical Location & Seq
Forward (+)
64738199 .. 64746052
7854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330940.1

Sequence Viewer

Length: 1308 bp
ATGAAGCTTGCAACTTGTTGTTCATATTCGTCGTCACCAACCTCCCTTACTCCTTTTCTCCTTCAAACCCGTCCCAGATTCTCATTCAATATATTTCAGCCCTCTTCTTCTTCTTCCTCAACCGCCGCCCACCGACCACGACCCGTCATGGTTTCCGCCCGCAACTACTCCCAGGCCCAGTATCTTCCTCCTTGGTTCAGTGTGGCTCCTATGATGGAATGGACTGATGTTCACTACAGGACTATGGCCCGCCTCATATCCAAGCATGCCTGGCTCTACACTGAGATGCTCGCTGCCGAAACCATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCGGAACAGCATCCTATTGTTCTACAAATTGGTGGCAGTAATTTGGAAAACCTGGCAAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTTGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCTGAGGCTATGTCAGTGATTGCTGCGAACACTGATGTCCCTGTCAGTGTTAAGTGTCGAATTGGTGTTGATGATCACGATTCATATAATGAGCTCTGTGATTTTATTTATAAAGTTTCTTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCCATGCTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTTAAATATGAGTACTTTTATGGCCTCTTGCGTGACTTTCCAGACTTAAGATTTACCATAAATGGTGGAATAAACACTATTGATGAGGTCAATGCAGCTAGGAAAGCAGGAGCTCATGGCGTAATGGTTGGACGGGCTGCATTTAATAACTCATGGAATACTTTGGGACATGTTGATACTGCTATTTATGGTGCACCAAGCAGCGGTTTAACACGGCGTCAGTTACTCAAGGAGTTTGAAGCCTATGGAGAGACTGTTTTGGGAAAATATGGACAAAAGCCCAATGTTCGAGATGTGGCCAAGCCTTTGCTTAGCCTTTTCTATGCTCAGCCTGGGAACAGCCTTTGGAAACGCAAAGCTGATGCTGCTTTCATGCATTGCACGACTATGAAATCATTTTTTGAGGAAACACTAGTAGCAATTCCAGACTTTGTCTTGGATGCACCTGTTGGAGCGCCACCATCTGGTAGTAATGATCCTTTTGCAAACATACATACTTTGTTGCCTCCACCATACGATTCGAGGGAACCATACGATTCGAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

435

Amino Acids

48.65

Weight (kDa)

8.03

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 68 - 375 2.8e-57 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 642
AccB7I CCANNNNNTGG 1 cut(s) 1205
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 6 cut(s) 123, 126, 156, 160, 250, 945
AclWI GGATC 3 cut(s) 512, 525, 1211
AcoI YGGCCR 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 958
AfaI GTAC 2 cut(s) 341, 755
AfiI CCNNNNNNNGG 2 cut(s) 944, 1205
AflII CTTAAG 1 cut(s) 787
AflIII ACRYGT 1 cut(s) 910
AgsI TTSAA 3 cut(s) 65, 88, 980
AhlI ACTAGT 1 cut(s) 1153
AjnI CCWGG 4 cut(s) 171, 269, 399, 1072
AluBI AGCT 9 cut(s) 7, 410, 482, 625, 719, 839, 854, 1100, 1294
AluI AGCT 9 cut(s) 7, 410, 482, 625, 719, 839, 854, 1100, 1294
Alw21I GWGCWC 4 cut(s) 505, 627, 856, 937
Alw26I GTCTC 1 cut(s) 986
Alw44I GTGCAC 1 cut(s) 933
AlwI GGATC 3 cut(s) 512, 525, 1211
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 5 cut(s) 174, 246, 693, 763, 1038
ApaLI GTGCAC 1 cut(s) 933
ApeKI GCWGC 6 cut(s) 293, 554, 836, 878, 942, 1106
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AspLEI GCGC 1 cut(s) 1198
AspS9I GGNCC 2 cut(s) 175, 247
AsuHPI GGTGA 2 cut(s) 27, 651
BaeGI GKGCMC 1 cut(s) 937
BalI TGGCCA 1 cut(s) 1040
BamHI GGATCC 1 cut(s) 517
BanII GRGCYC 2 cut(s) 627, 856
Bbv12I GWGCWC 4 cut(s) 505, 627, 856, 937
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 6 cut(s) 280, 541, 848, 865, 954, 1093
BccI CCATC 2 cut(s) 208, 1210
BceAI ACGGC 1 cut(s) 971
BciT130I CCWGG 4 cut(s) 173, 271, 401, 1074
BclI TGATCA 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 986
BcuI ACTAGT 1 cut(s) 1153
BfaI CTAG 3 cut(s) 666, 840, 1154
BfmI CTRYAG 1 cut(s) 235
BfoI RGCGCY 1 cut(s) 1199
BfrI CTTAAG 1 cut(s) 787
BisI GCNGC 7 cut(s) 126, 294, 555, 837, 879, 943, 1107
BlpI GCTNAGC 2 cut(s) 1052, 1068
BlsI GCNGC 7 cut(s) 127, 295, 556, 838, 880, 944, 1108
BmcAI AGTACT 1 cut(s) 755
Bme1390I CCNGG 4 cut(s) 173, 271, 401, 1074
BmgT120I GGNCC 2 cut(s) 175, 247
BmiI GGNNCC 3 cut(s) 207, 519, 1269
BmrFI CCNGG 4 cut(s) 173, 271, 401, 1074
BmrI ACTGGG 2 cut(s) 172, 462
BmsI GCATC 4 cut(s) 276, 367, 1093, 1171
BmuI ACTGGG 2 cut(s) 172, 462
Bpu10I CCTNAGC 1 cut(s) 534
Bpu1102I GCTNAGC 2 cut(s) 1052, 1068
BpuEI CTTGAG 1 cut(s) 953
BsaHI GRCGYC 1 cut(s) 958
BsaJI CCNNGG 3 cut(s) 171, 191, 1073
BsaWI WCCGGW 1 cut(s) 349
BsaXI ACNNNNNCTCC 2 cut(s) 981, 1011
Bsc4I CCNNNNNNNGG 2 cut(s) 944, 1205
Bse1I ACTGG 2 cut(s) 178, 468
Bse3DI GCAATG 1 cut(s) 1117
BseAI TCCGGA 1 cut(s) 349
BseBI CCWGG 4 cut(s) 173, 271, 401, 1074
BseDI CCNNGG 3 cut(s) 171, 191, 1073
BseGI GGATG 3 cut(s) 358, 467, 1186
BseLI CCNNNNNNNGG 2 cut(s) 944, 1205
BseMI GCAATG 1 cut(s) 1117
BseMII CTCAG 3 cut(s) 273, 525, 1082
BseNI ACTGG 2 cut(s) 178, 468
BseSI GKGCMC 1 cut(s) 937
BseXI GCAGC 6 cut(s) 280, 541, 848, 865, 954, 1093
BseYI CCCAGC 1 cut(s) 715
BshFI GGCC 5 cut(s) 176, 248, 695, 765, 1040
BsiHKAI GWGCWC 4 cut(s) 505, 627, 856, 937
BsiSI CCGG 1 cut(s) 350
BslFI GGGAC 4 cut(s) 57, 450, 554, 921
BslI CCNNNNNNNGG 2 cut(s) 944, 1205
BsmAI GTCTC 1 cut(s) 986
BsmFI GGGAC 4 cut(s) 57, 450, 554, 921
BsnI GGCC 5 cut(s) 176, 248, 695, 765, 1040
Bsp1286I GDGCHC 4 cut(s) 505, 627, 856, 937
Bsp13I TCCGGA 1 cut(s) 349
Bsp143I GATC 4 cut(s) 444, 517, 604, 1216
Bsp1720I GCTNAGC 2 cut(s) 1052, 1068
BspACI CCGC 6 cut(s) 123, 126, 156, 160, 250, 945
BspANI GGCC 5 cut(s) 176, 248, 695, 765, 1040
BspCNI CTCAG 3 cut(s) 274, 526, 1081
BspEI TCCGGA 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 207, 519, 1269
BspPI GGATC 3 cut(s) 512, 525, 1211
BspTI CTTAAG 1 cut(s) 787
BsrDI GCAATG 1 cut(s) 1117
BsrI ACTGG 2 cut(s) 178, 468
BssECI CCNNGG 3 cut(s) 171, 191, 1073
BssMI GATC 4 cut(s) 444, 517, 604, 1216
BssNI GRCGYC 1 cut(s) 958
BssT1I CCWWGG 1 cut(s) 191
Bst2UI CCWGG 4 cut(s) 173, 271, 401, 1074
Bst4CI ACNGT 1 cut(s) 997
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 958
BstAFI CTTAAG 1 cut(s) 787
BstC8I GCNNGC 5 cut(s) 9, 160, 250, 267, 291
BstDEI CTNAG 5 cut(s) 282, 534, 1052, 1068, 1305
BstF5I GGATG 3 cut(s) 358, 467, 1186
BstH2I RGCGCY 1 cut(s) 1199
BstHHI GCGC 1 cut(s) 1198
BstKTI GATC 4 cut(s) 447, 520, 607, 1219
BstMAI GTCTC 1 cut(s) 986
BstMBI GATC 4 cut(s) 444, 517, 604, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 271, 845, 1106
BstNI CCWGG 4 cut(s) 173, 271, 401, 1074
BstNSI RCATGY 2 cut(s) 269, 914
BstSCI CCNGG 4 cut(s) 171, 269, 399, 1072
BstSFI CTRYAG 1 cut(s) 235
BstSLI GKGCMC 1 cut(s) 937
BstV1I GCAGC 6 cut(s) 280, 541, 848, 865, 954, 1093
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BsuRI GGCC 5 cut(s) 176, 248, 695, 765, 1040
BtsCI GGATG 3 cut(s) 358, 467, 1186
BtsIMutI CAGTG 5 cut(s) 205, 279, 552, 561, 583
Cac8I GCNNGC 5 cut(s) 9, 160, 250, 267, 291
CfoI GCGC 1 cut(s) 1198
Cfr13I GGNCC 2 cut(s) 175, 247
CseI GACGC 1 cut(s) 947
Csp6I GTAC 2 cut(s) 340, 754
CviAII CATG 8 cut(s) 148, 266, 488, 697, 857, 894, 911, 1114
CviQI GTAC 2 cut(s) 340, 754
DdeI CTNAG 5 cut(s) 282, 534, 1052, 1068, 1305
DpnI GATC 4 cut(s) 446, 519, 606, 1218
DpnII GATC 4 cut(s) 444, 517, 604, 1216
EaeI YGGCCR 1 cut(s) 1038
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 2 cut(s) 625, 854
Eco130I CCWWGG 1 cut(s) 191
Eco24I GRGCYC 2 cut(s) 627, 856
Eco53kI GAGCTC 2 cut(s) 625, 854
EcoICRI GAGCTC 2 cut(s) 625, 854
EcoRII CCWGG 4 cut(s) 171, 269, 399, 1072
EcoT14I CCWWGG 1 cut(s) 191
EcoT22I ATGCAT 1 cut(s) 1119
EcoT38I GRGCYC 2 cut(s) 627, 856
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 8 cut(s) 151, 269, 491, 700, 860, 897, 914, 1117
FaqI GGGAC 4 cut(s) 57, 450, 554, 921
FatI CATG 8 cut(s) 147, 265, 487, 696, 856, 893, 910, 1113
FauI CCCGC 2 cut(s) 167, 257
FbaI TGATCA 1 cut(s) 604
Fnu4HI GCNGC 7 cut(s) 126, 294, 555, 837, 879, 943, 1107
FokI GGATG 3 cut(s) 345, 474, 1193
FriOI GRGCYC 2 cut(s) 627, 856
Fsp4HI GCNGC 7 cut(s) 126, 294, 555, 837, 879, 943, 1107
FspBI CTAG 3 cut(s) 666, 840, 1154
GlaI GCGC 1 cut(s) 1197
GluI GCNGC 7 cut(s) 126, 294, 555, 837, 879, 943, 1107
GsaI CCCAGC 1 cut(s) 719
HaeII RGCGCY 1 cut(s) 1199
HaeIII GGCC 5 cut(s) 176, 248, 695, 765, 1040
HapII CCGG 1 cut(s) 350
HgaI GACGC 1 cut(s) 947
HhaI GCGC 1 cut(s) 1198
Hin1I GRCGYC 1 cut(s) 958
Hin1II CATG 8 cut(s) 151, 269, 491, 700, 860, 897, 914, 1117
Hin6I GCGC 1 cut(s) 1196
HinP1I GCGC 1 cut(s) 1196
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 6 cut(s) 78, 333, 611, 708, 1259, 1277
HpaII CCGG 1 cut(s) 350
HphI GGTGA 2 cut(s) 27, 651
Hpy166II GTNNAC 2 cut(s) 232, 935
Hpy188I TCNGA 2 cut(s) 315, 690
Hpy188III TCNNGA 6 cut(s) 326, 350, 608, 782, 1031, 1166
Hpy8I GTNNAC 2 cut(s) 232, 935
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 3 cut(s) 71, 310, 685
HpyCH4III ACNGT 1 cut(s) 997
HpyCH4V TGCA 8 cut(s) 11, 836, 881, 935, 1117, 1122, 1184, 1226
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 845, 1106
HpyF3I CTNAG 5 cut(s) 282, 534, 1052, 1068, 1305
Hsp92I GRCGYC 1 cut(s) 958
Hsp92II CATG 8 cut(s) 151, 269, 491, 700, 860, 897, 914, 1117
HspAI GCGC 1 cut(s) 1196
Kpn2I TCCGGA 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 4 cut(s) 444, 517, 604, 1216
LmnI GCTCC 3 cut(s) 211, 851, 1193
Lsp1109I GCAGC 6 cut(s) 280, 541, 848, 865, 954, 1093
LweI GCATC 4 cut(s) 276, 367, 1093, 1171
MaeI CTAG 3 cut(s) 666, 840, 1154
MaeIII GTNAC 3 cut(s) 33, 773, 963
MalI GATC 4 cut(s) 446, 519, 606, 1218
MboI GATC 4 cut(s) 444, 517, 604, 1216
MboII GAAGA 6 cut(s) 96, 99, 102, 105, 176, 642
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 4 cut(s) 505, 627, 856, 937
MlsI TGGCCA 1 cut(s) 1040
MluCI AATT 6 cut(s) 375, 388, 448, 454, 591, 1161
MluNI TGGCCA 1 cut(s) 1040
MmeI TCCRAC 2 cut(s) 850, 1171
Mox20I TGGCCA 1 cut(s) 1040
Mph1103I ATGCAT 1 cut(s) 1119
MroI TCCGGA 1 cut(s) 349
MscI TGGCCA 1 cut(s) 1040
MseI TTAA 6 cut(s) 453, 582, 744, 788, 885, 950
MslI CAYNNNNRTG 2 cut(s) 284, 1127
Msp20I TGGCCA 1 cut(s) 1040
MspA1I CMGCKG 2 cut(s) 719, 945
MspCI CTTAAG 1 cut(s) 787
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 4 cut(s) 173, 271, 401, 1074
MvaI CCWGG 4 cut(s) 173, 271, 401, 1074
MwoI GCNNNNNNNGC 3 cut(s) 271, 845, 1106
NdeII GATC 4 cut(s) 444, 517, 604, 1216
NlaIII CATG 8 cut(s) 151, 269, 491, 700, 860, 897, 914, 1117
NlaIV GGNNCC 3 cut(s) 207, 519, 1269
NmuCI GTSAC 2 cut(s) 33, 773
NsiI ATGCAT 1 cut(s) 1119
NspI RCATGY 2 cut(s) 269, 914
PaeI GCATGC 1 cut(s) 269
PciI ACATGT 1 cut(s) 910
PfeI GAWTC 6 cut(s) 78, 333, 611, 708, 1259, 1277
PflFI GACNNNGTC 1 cut(s) 1172
PflMI CCANNNNNTGG 1 cut(s) 1205
PkrI GCNGC 7 cut(s) 127, 295, 556, 838, 880, 944, 1108
PscI ACATGT 1 cut(s) 910
PsiI TTATAA 1 cut(s) 642
Psp124BI GAGCTC 2 cut(s) 627, 856
Psp6I CCWGG 4 cut(s) 171, 269, 399, 1072
PspFI CCCAGC 1 cut(s) 715
PspGI CCWGG 4 cut(s) 171, 269, 399, 1072
PspN4I GGNNCC 3 cut(s) 207, 519, 1269
PspPI GGNCC 2 cut(s) 175, 247
PsrI GAACNNNNNNTAC 1 cut(s) 1278
PsuI RGATCY 1 cut(s) 517
PsyI GACNNNGTC 1 cut(s) 1172
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 2 cut(s) 341, 755
RsaNI GTAC 2 cut(s) 340, 754
RseI CAYNNNNRTG 2 cut(s) 284, 1127
SacI GAGCTC 2 cut(s) 627, 856
SaqAI TTAA 6 cut(s) 453, 582, 744, 788, 885, 950
SatI GCNGC 7 cut(s) 126, 294, 555, 837, 879, 943, 1107
Sau3AI GATC 4 cut(s) 444, 517, 604, 1216
Sau96I GGNCC 2 cut(s) 175, 247
ScaI AGTACT 1 cut(s) 755
ScrFI CCNGG 4 cut(s) 173, 271, 401, 1074
SduI GDGCHC 4 cut(s) 505, 627, 856, 937
SfaNI GCATC 4 cut(s) 276, 367, 1093, 1171
SfcI CTRYAG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 284, 1127
SmlI CTYRAG 2 cut(s) 787, 968
SmoI CTYRAG 2 cut(s) 787, 968
SpeI ACTAGT 1 cut(s) 1153
SphI GCATGC 1 cut(s) 269
Sse9I AATT 6 cut(s) 375, 388, 448, 454, 591, 1161
SsiI CCGC 6 cut(s) 123, 126, 156, 160, 250, 945
SspI AATATT 1 cut(s) 427
SspMI CTAG 3 cut(s) 666, 840, 1154
SstI GAGCTC 2 cut(s) 627, 856
StyD4I CCNGG 4 cut(s) 171, 269, 399, 1072
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 1 cut(s) 997
TaqI TCGA 5 cut(s) 589, 727, 1030, 1262, 1280
TasI AATT 6 cut(s) 375, 388, 448, 454, 591, 1161
TatI WGTACW 2 cut(s) 339, 753
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 6 cut(s) 78, 333, 611, 708, 1259, 1277
Tru1I TTAA 6 cut(s) 453, 582, 744, 788, 885, 950
Tru9I TTAA 6 cut(s) 453, 582, 744, 788, 885, 950
TscAI CASTG 5 cut(s) 205, 286, 552, 568, 583
TseFI GTSAC 2 cut(s) 33, 773
TseI GCWGC 6 cut(s) 293, 554, 836, 878, 942, 1106
Tsp45I GTSAC 2 cut(s) 33, 773
TspDTI ATGAA 6 cut(s) 12, 17, 603, 664, 1102, 1145
TspRI CASTG 5 cut(s) 205, 286, 552, 568, 583
Tth111I GACNNNGTC 1 cut(s) 1172
Van91I CCANNNNNTGG 1 cut(s) 1205
Vha464I CTTAAG 1 cut(s) 787
VneI GTGCAC 1 cut(s) 933
XceI RCATGY 2 cut(s) 269, 914
XspI CTAG 3 cut(s) 666, 840, 1154
ZrmI AGTACT 1 cut(s) 755
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.