AT5G49945

Protein of unknown function (DUF1682)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007803
Physical Location & Seq
Forward (+)
20317405 .. 20319830
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49945.1

Sequence Viewer

Length: 1443 bp
ATGAGCCGATATTTCTCCTTCTTCTTCTTAGCTCTCTTCCTCCATTATCGTATTATCGTAGCTTCTCCATTCGAAGGATTTGATGCTGAAGAAGACGACGTTACCGACGATTCCTCTCACCTTCTTCACCACTCACTCCCTCCGCCTCTTCTCACTCAGTCTCACTCATCACTCTCCGATCCCGATCCGGAGCCGGAACCTTCTTCCGCCGAATGTAAATCCGATCTCATCACTGAATCAGATCTCGAGCATCAATCCGATTCGAAAACTCCCTCGTCTACACCATTTGAGTACTGGGACGAAGACGAGTTCGAAGGACTACCAGTTGAGATAGAGACACTTGAATCTCCATTGATTACAGAAAACGGAACTCATGCTGATCCAAAGACGCCAGATCTCAAGACTTCATCGGAAGCTCAAGGAGACACTAATGATCAAACGAAGAAGAAGAAGTCGTACGCGGTGGAGATCGCTTGCGTCTGTTTCTTAATCGCACTCGCAATCAACTACTTCGTTGGTAAACGTGAGAACGAGAGTCTCGCATTAGCTTGGGCGGCGAAATTTGCTTCTAAGGATACAATATTCCAGAAGAATTTTAGTATGCTTGGAGTTAGTGAATTAGAGGATTCGCCATTGTTGTTAAAAGAAGCCTTAAATGTGTTCAAATTCTACGCGAGTGGTCGTAGGTATTGCCATGGATTGTTAGCTACTATGGAGCTTAAGAGTAGACATGATTTGATCTCTAGGGTGTTTAATTTGGTTGTTCCTTGTAAAGATGAGATCACTTTTGAGGTTTATATGAATGAAGAAACTATGGATCATGTTGTGTTTGCTATGACGAAGAAGAAGGCTGCAAAAACTATGCAAAAGGAGATGAGGGATTTGCAAAGATTTGCTGGGATTGTGTCTCCTCCTGCTGGAAGGAAATGGGTGTCGGAGGAGTTCGCCTTGATCTCTGAATCCAAGGAAGTTGCTGCGGATTTGATCACTGACACTGTTCTTGATCAGGTTTTTGGTGACAAGGCTGTGGATAAGTATGGGAAGAATTTCATGTCGATGCATATATCTGACCAACACCCTGGCAAGCACAAGAAGATGATGCTTTTCAAGTTTTCTTTACCTGATGCTAAACATATGGATGATATTGTCCGGTTGGTGGCGCTAATTCCATATTACATCGACTTAGTAGGACGATACAGACTCAGCTCGCAGGCGCGGAACAAAACTGAGAGTGGTAGGCAAAAGGCAGCAGAGGAAGCATACAAGGAACTTCATAACGCGAGGCAAGAGGCATTGCAGAAGAAGAAAGCAGAAAAGAAGAAAATGATGGAGGAAGCGGAAGCAAAGATGAGCGCAGAAGTGATAAGGAAGAAAGAGGCAAAAGAGAGAGCAAGGCAGGTGAAAAAGGCAGTACCAAAAATGAAGATGAGCCGTAGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

480

Amino Acids

54.59

Weight (kDa)

6.02

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC47 PF07946 152 - 469 1.6e-82 PAT complex subunit CCDC47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1387
Acc36I ACCTGC 1 cut(s) 1387
AccI GTMKAC 2 cut(s) 278, 727
AccII CGCG 4 cut(s) 461, 674, 1216, 1280
AccIII TCCGGA 1 cut(s) 187
AciI CCGC 7 cut(s) 143, 207, 461, 554, 977, 1216, 1337
AclWI GGATC 4 cut(s) 173, 179, 374, 825
AcsI RAATTY 4 cut(s) 560, 592, 665, 1045
AcuI CTGAAG 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 3 cut(s) 293, 458, 1413
AfiI CCNNNNNNNGG 3 cut(s) 74, 917, 1156
AflII CTTAAG 1 cut(s) 719
AgsI TTSAA 3 cut(s) 344, 664, 1108
AjnI CCWGG 1 cut(s) 1078
AluBI AGCT 7 cut(s) 32, 62, 416, 548, 707, 718, 1206
AluI AGCT 7 cut(s) 32, 62, 416, 548, 707, 718, 1206
Alw26I GTCTC 5 cut(s) 165, 329, 417, 542, 912
AlwI GGATC 4 cut(s) 173, 179, 374, 825
Ama87I CYCGRG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 187
ApeKI GCWGC 3 cut(s) 851, 974, 1247
ApoI RAATTY 4 cut(s) 560, 592, 665, 1045
Asp700I GAANNNNTTC 1 cut(s) 1046
AspLEI GCGC 3 cut(s) 1162, 1216, 1355
AsuHPI GGTGA 4 cut(s) 110, 119, 1028, 1411
AsuII TTCGAA 3 cut(s) 72, 263, 312
AvaI CYCGRG 1 cut(s) 245
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BbsI GAAGAC 2 cut(s) 99, 309
BbvI GCAGC 3 cut(s) 838, 961, 1259
BccI CCATC 1 cut(s) 1321
BceAI ACGGC 1 cut(s) 1416
BciT130I CCWGG 1 cut(s) 1080
BciVI GTATCC 1 cut(s) 568
BclI TGATCA 3 cut(s) 433, 984, 1003
BcoDI GTCTC 5 cut(s) 165, 329, 417, 542, 912
BfaI CTAG 2 cut(s) 744, 1441
BfoI RGCGCY 1 cut(s) 1163
BfrI CTTAAG 1 cut(s) 719
BfuAI ACCTGC 1 cut(s) 1387
BfuI GTATCC 1 cut(s) 568
BglII AGATCT 2 cut(s) 241, 394
BisI GCNGC 4 cut(s) 555, 852, 975, 1248
BlsI GCNGC 4 cut(s) 556, 853, 976, 1249
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 1 cut(s) 1080
BmeT110I CYCGRG 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 192, 198
BmrFI CCNGG 1 cut(s) 1080
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 5 cut(s) 73, 259, 1047, 1089, 1114
BmuI ACTGGG 1 cut(s) 304
BpiI GAAGAC 2 cut(s) 99, 309
Bpu14I TTCGAA 3 cut(s) 72, 263, 312
BpuEI CTTGAG 2 cut(s) 383, 402
BsaBI GATNNNNATC 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 3 cut(s) 694, 963, 1078
BsaWI WCCGGW 2 cut(s) 187, 1149
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 917, 1156
Bse1I ACTGG 2 cut(s) 299, 323
Bse3DI GCAATG 1 cut(s) 1292
Bse8I GATNNNNATC 1 cut(s) 183
BseAI TCCGGA 1 cut(s) 187
BseBI CCWGG 1 cut(s) 1080
BseDI CCNNGG 3 cut(s) 694, 963, 1078
BseGI GGATG 1 cut(s) 1144
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 3 cut(s) 74, 917, 1156
BseMI GCAATG 1 cut(s) 1292
BseMII CTCAG 3 cut(s) 170, 1216, 1218
BseNI ACTGG 2 cut(s) 299, 323
BseRI GAGGAG 2 cut(s) 900, 953
BseXI GCAGC 3 cut(s) 838, 961, 1259
BseYI CCCAGC 1 cut(s) 896
Bsh1236I CGCG 4 cut(s) 461, 674, 1216, 1280
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 3 cut(s) 188, 194, 1150
BsiWI CGTACG 1 cut(s) 456
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 3 cut(s) 74, 917, 1156
BsmAI GTCTC 5 cut(s) 165, 329, 417, 542, 912
BsmFI GGGAC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 245
Bsp119I TTCGAA 3 cut(s) 72, 263, 312
Bsp13I TCCGGA 1 cut(s) 187
Bsp19I CCATGG 1 cut(s) 694
BspACI CCGC 7 cut(s) 143, 207, 461, 554, 977, 1216, 1337
BspCNI CTCAG 3 cut(s) 169, 1215, 1219
BspEI TCCGGA 1 cut(s) 187
BspFNI CGCG 4 cut(s) 461, 674, 1216, 1280
BspLI GGNNCC 2 cut(s) 192, 198
BspMI ACCTGC 1 cut(s) 1387
BspPI GGATC 4 cut(s) 173, 179, 374, 825
BspT104I TTCGAA 3 cut(s) 72, 263, 312
BspTI CTTAAG 1 cut(s) 719
BsrDI GCAATG 1 cut(s) 1292
BsrI ACTGG 2 cut(s) 299, 323
BssECI CCNNGG 3 cut(s) 694, 963, 1078
BssNI GRCGYC 1 cut(s) 389
BssT1I CCWWGG 2 cut(s) 694, 963
Bst2UI CCWGG 1 cut(s) 1080
Bst4CI ACNGT 1 cut(s) 997
Bst6I CTCTTC 2 cut(s) 41, 153
BstACI GRCGYC 1 cut(s) 389
BstAFI CTTAAG 1 cut(s) 719
BstBI TTCGAA 3 cut(s) 72, 263, 312
BstC8I GCNNGC 4 cut(s) 475, 1085, 1208, 1212
BstDEI CTNAG 6 cut(s) 28, 156, 570, 1183, 1202, 1227
BstDSI CCRYGG 1 cut(s) 694
BstF5I GGATG 1 cut(s) 1144
BstFNI CGCG 4 cut(s) 461, 674, 1216, 1280
BstH2I RGCGCY 1 cut(s) 1163
BstHHI GCGC 3 cut(s) 1162, 1216, 1355
BstMAI GTCTC 5 cut(s) 165, 329, 417, 542, 912
BstMWI GCNNNNNNNGC 3 cut(s) 554, 563, 1256
BstNI CCWGG 1 cut(s) 1080
BstSCI CCNGG 1 cut(s) 1078
BstUI CGCG 4 cut(s) 461, 674, 1216, 1280
BstV1I GCAGC 3 cut(s) 838, 961, 1259
BstV2I GAAGAC 2 cut(s) 99, 309
BstX2I RGATCY 2 cut(s) 241, 394
BstXI CCANNNNNNTGG 1 cut(s) 1079
BstYI RGATCY 2 cut(s) 241, 394
BsuI GTATCC 1 cut(s) 568
BtgI CCRYGG 1 cut(s) 694
BtsCI GGATG 1 cut(s) 1144
BtsIMutI CAGTG 3 cut(s) 231, 987, 993
BveI ACCTGC 1 cut(s) 1387
Cac8I GCNNGC 4 cut(s) 475, 1085, 1208, 1212
CfoI GCGC 3 cut(s) 1162, 1216, 1355
CseI GACGC 2 cut(s) 397, 466
Csp6I GTAC 3 cut(s) 292, 457, 1412
CviAII CATG 5 cut(s) 374, 695, 731, 821, 1051
CviQI GTAC 3 cut(s) 292, 457, 1412
DdeI CTNAG 6 cut(s) 28, 156, 570, 1183, 1202, 1227
Eam1104I CTCTTC 2 cut(s) 41, 153
EarI CTCTTC 2 cut(s) 41, 153
EciI GGCGGA 2 cut(s) 132, 196
Eco130I CCWWGG 2 cut(s) 694, 963
Eco57I CTGAAG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 1078
EcoT14I CCWWGG 2 cut(s) 694, 963
EcoT22I ATGCAT 1 cut(s) 1062
ErhI CCWWGG 2 cut(s) 694, 963
FaeI CATG 5 cut(s) 377, 698, 734, 824, 1054
FaqI GGGAC 1 cut(s) 311
FatI CATG 5 cut(s) 373, 694, 730, 820, 1050
FauNDI CATATG 1 cut(s) 1134
FbaI TGATCA 3 cut(s) 433, 984, 1003
FblI GTMKAC 2 cut(s) 278, 727
Fnu4HI GCNGC 4 cut(s) 555, 852, 975, 1248
FokI GGATG 1 cut(s) 1151
Fsp4HI GCNGC 4 cut(s) 555, 852, 975, 1248
FspBI CTAG 2 cut(s) 744, 1441
GlaI GCGC 3 cut(s) 1161, 1215, 1354
GluI GCNGC 4 cut(s) 555, 852, 975, 1248
GsaI CCCAGC 1 cut(s) 900
HaeII RGCGCY 1 cut(s) 1163
HapII CCGG 3 cut(s) 188, 194, 1150
HgaI GACGC 2 cut(s) 397, 466
HhaI GCGC 3 cut(s) 1162, 1216, 1355
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 5 cut(s) 377, 698, 734, 824, 1054
Hin6I GCGC 3 cut(s) 1160, 1214, 1353
HinP1I GCGC 3 cut(s) 1160, 1214, 1353
HinfI GANTC 8 cut(s) 110, 236, 260, 344, 535, 626, 959, 1200
HpaII CCGG 3 cut(s) 188, 194, 1150
HphI GGTGA 4 cut(s) 110, 119, 1028, 1411
Hpy166II GTNNAC 3 cut(s) 279, 521, 728
Hpy188I TCNGA 8 cut(s) 178, 223, 241, 259, 412, 937, 958, 1069
Hpy188III TCNNGA 6 cut(s) 182, 188, 245, 400, 586, 1001
Hpy8I GTNNAC 3 cut(s) 279, 521, 728
Hpy99I CGWCG 2 cut(s) 101, 110
HpyAV CCTTC 7 cut(s) 28, 68, 131, 210, 308, 841, 915
HpyCH4III ACNGT 1 cut(s) 997
HpyCH4IV ACGT 2 cut(s) 99, 523
HpyCH4V TGCA 5 cut(s) 854, 865, 886, 1060, 1297
HpyF10VI GCNNNNNNNGC 3 cut(s) 554, 563, 1256
HpyF3I CTNAG 6 cut(s) 28, 156, 570, 1183, 1202, 1227
HpySE526I ACGT 2 cut(s) 99, 523
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 5 cut(s) 377, 698, 734, 824, 1054
HspAI GCGC 3 cut(s) 1160, 1214, 1353
Kpn2I TCCGGA 1 cut(s) 187
Ksp22I TGATCA 3 cut(s) 433, 984, 1003
LmnI GCTCC 2 cut(s) 190, 715
Lsp1109I GCAGC 3 cut(s) 838, 961, 1259
LweI GCATC 5 cut(s) 73, 259, 1047, 1089, 1114
MaeI CTAG 2 cut(s) 744, 1441
MaeII ACGT 2 cut(s) 99, 523
MaeIII GTNAC 3 cut(s) 100, 1016, 1436
MflI RGATCY 2 cut(s) 241, 394
MluCI AATT 7 cut(s) 560, 592, 617, 665, 754, 1045, 1164
MlyI GAGTC 2 cut(s) 544, 1194
MmeI TCCRAC 1 cut(s) 915
Mph1103I ATGCAT 1 cut(s) 1062
MroI TCCGGA 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 1046
MseI TTAA 5 cut(s) 488, 641, 653, 720, 753
MslI CAYNNNNRTG 2 cut(s) 1055, 1137
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 3 cut(s) 188, 194, 1150
MspR9I CCNGG 1 cut(s) 1080
MvaI CCWGG 1 cut(s) 1080
MvnI CGCG 4 cut(s) 461, 674, 1216, 1280
MwoI GCNNNNNNNGC 3 cut(s) 554, 563, 1256
NcoI CCATGG 1 cut(s) 694
NdeI CATATG 1 cut(s) 1134
NlaIII CATG 5 cut(s) 377, 698, 734, 824, 1054
NlaIV GGNNCC 2 cut(s) 192, 198
NmuCI GTSAC 2 cut(s) 1016, 1436
NsiI ATGCAT 1 cut(s) 1062
NspV TTCGAA 3 cut(s) 72, 263, 312
PaeR7I CTCGAG 1 cut(s) 245
PaqCI CACCTGC 1 cut(s) 1387
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 105
PdmI GAANNNNTTC 1 cut(s) 1046
PfeI GAWTC 6 cut(s) 110, 236, 260, 344, 626, 959
Pfl23II CGTACG 1 cut(s) 456
PkrI GCNGC 4 cut(s) 556, 853, 976, 1249
PleI GAGTC 2 cut(s) 543, 1194
PpsI GAGTC 2 cut(s) 543, 1194
Psp6I CCWGG 1 cut(s) 1078
PspFI CCCAGC 1 cut(s) 896
PspGI CCWGG 1 cut(s) 1078
PspLI CGTACG 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 192, 198
PsuI RGATCY 2 cut(s) 241, 394
RsaI GTAC 3 cut(s) 293, 458, 1413
RsaNI GTAC 3 cut(s) 292, 457, 1412
RseI CAYNNNNRTG 2 cut(s) 1055, 1137
SaqAI TTAA 5 cut(s) 488, 641, 653, 720, 753
SatI GCNGC 4 cut(s) 555, 852, 975, 1248
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 2 cut(s) 544, 1194
ScrFI CCNGG 1 cut(s) 1080
SfaNI GCATC 5 cut(s) 73, 259, 1047, 1089, 1114
Sfr274I CTCGAG 1 cut(s) 245
SfuI TTCGAA 3 cut(s) 72, 263, 312
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 1055, 1137
SmlI CTYRAG 4 cut(s) 245, 398, 417, 719
SmoI CTYRAG 4 cut(s) 245, 398, 417, 719
Sse9I AATT 7 cut(s) 560, 592, 617, 665, 754, 1045, 1164
SsiI CCGC 7 cut(s) 143, 207, 461, 554, 977, 1216, 1337
SspI AATATT 1 cut(s) 582
SspMI CTAG 2 cut(s) 744, 1441
StyD4I CCNGG 1 cut(s) 1078
StyI CCWWGG 2 cut(s) 694, 963
TaaI ACNGT 1 cut(s) 997
TaiI ACGT 2 cut(s) 102, 526
TaqI TCGA 6 cut(s) 72, 246, 263, 312, 1055, 1179
TasI AATT 7 cut(s) 560, 592, 617, 665, 754, 1045, 1164
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 557
TfiI GAWTC 6 cut(s) 110, 236, 260, 344, 626, 959
Tru1I TTAA 5 cut(s) 488, 641, 653, 720, 753
Tru9I TTAA 5 cut(s) 488, 641, 653, 720, 753
TscAI CASTG 3 cut(s) 238, 994, 1000
TseFI GTSAC 2 cut(s) 1016, 1436
TseI GCWGC 3 cut(s) 851, 974, 1247
Tsp45I GTSAC 2 cut(s) 1016, 1436
TspDTI ATGAA 6 cut(s) 396, 815, 819, 1039, 1262, 1436
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 3 cut(s) 238, 994, 1000
Vha464I CTTAAG 1 cut(s) 719
XapI RAATTY 4 cut(s) 560, 592, 665, 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
XhoI CTCGAG 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 278, 727
XmnI GAANNNNTTC 1 cut(s) 1046
XspI CTAG 2 cut(s) 744, 1441
ZrmI AGTACT 1 cut(s) 293
Zsp2I ATGCAT 1 cut(s) 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.