pycom08g20860

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007803
Physical Location & Seq
Forward (+)
20492600 .. 20493448
849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20860.1

Sequence Viewer

Length: 849 bp
ATGGCGTCACTCTCTTACACGCGCCTCCTCTCTCTCCTCTCCCTCTTCATCCTCCTGTTCCTCTTCTCCCACCTGTCCTTCTCTCCCTCCTCCGTCCTCGCCGCCGACTCGCAATTCGAAGGATTCGACGATGGCGACGAAATCGATGACGCCGACGCCGACGCCGACGACTCCTCACTCCCTCTTCCCACTCGCACCCGCCCTTCCCTCACCACCACCTCCCAACCCGACCCACCTCGCTCACCTGAACCCTCCGTACCCGACACGGATCCCAACCCGCTTCCCGATTCGGATTCTCCATCCACCGATCTTCCCAAACCTTCCTCCACCAGCTTCGATTATTGGGACGAAGACGAGTTCGAAGGCCTCCCCGTCCAACCCCAGCAACCGCAGCAGAAACCGCCCCTCGAAGACCCTGCAAGTACCGAAAATGCCACTCCCTCCTCCAATTCCGATCCCAAACCCTCCGCCGTTCCCAAGCCCCCCGCCGCTCCGAGATCCTTTGTTGTGGAGATCGCGTGCGGAGGGTTCCTGATCGTCTTCATCATCAACTACTTCACTGGGAAGCGGGTGAACGAGAACATTGCCCTGGCCTGGGCGGCGAAATTCGCCACCAAAGACTCGATTTTCGAGAAGAACTTCAGTCTTCTGGGCGTCGGAGACGGAGACGACTCACCCTTGCTGTTGAAGGAAGGTCAGAACGTGTTCAAGTTCTACGCGAGTGGGCGGAGGTGCTGCCAGGGGCTTCTGGCGACGATGGAGCTCAAAAGCCGCCACGATTTGATCGATCCTCGATTTTCAACTTGGTGGTCCCCAGTAGGGACGAGATCAATATCGAGGTTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

283

Amino Acids

30.85

Weight (kDa)

4.41

Isoelectric Point (pI)

58.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC47 PF07946 167 - 263 3.2e-29 PAT complex subunit CCDC47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 491
AccII CGCG 3 cut(s) 22, 518, 719
AclWI GGATC 5 cut(s) 263, 276, 449, 492, 782
AcsI RAATTY 1 cut(s) 605
AcuI CTGAAG 1 cut(s) 625
AcyI GRCGYC 5 cut(s) 5, 150, 156, 162, 654
AfaI GTAC 2 cut(s) 258, 424
AfiI CCNNNNNNNGG 3 cut(s) 594, 595, 819
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 3 cut(s) 688, 709, 801
AjnI CCWGG 3 cut(s) 588, 593, 738
AluBI AGCT 2 cut(s) 333, 763
AluI AGCT 2 cut(s) 333, 763
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 2 cut(s) 654, 660
AlwI GGATC 5 cut(s) 263, 276, 449, 492, 782
AoxI GGCC 2 cut(s) 364, 591
ApeKI GCWGC 2 cut(s) 391, 735
ApoI RAATTY 1 cut(s) 605
ArsI GACNNNNNNTTYG 4 cut(s) 98, 130, 611, 643
Asp700I GAANNNNTTC 2 cut(s) 638, 704
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 810
AsuHPI GGTGA 4 cut(s) 202, 234, 583, 666
AsuII TTCGAA 2 cut(s) 117, 360
AvaII GGWCC 1 cut(s) 810
BamHI GGATCC 1 cut(s) 268
BanII GRGCYC 1 cut(s) 765
BbsI GAAGAC 4 cut(s) 357, 417, 532, 638
Bbv12I GWGCWC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 403, 722
BccI CCATC 3 cut(s) 125, 307, 751
BceAI ACGGC 1 cut(s) 455
BcgI CGANNNNNNTGC 4 cut(s) 398, 432, 566, 600
BciT130I CCWGG 3 cut(s) 590, 595, 740
BcoDI GTCTC 2 cut(s) 654, 660
BglI GCCNNNNNGGC 1 cut(s) 599
BisI GCNGC 6 cut(s) 102, 392, 489, 600, 736, 772
BlsI GCNGC 6 cut(s) 103, 393, 490, 601, 737, 773
Bme1390I CCNGG 3 cut(s) 590, 595, 740
Bme18I GGWCC 1 cut(s) 810
BmgT120I GGNCC 1 cut(s) 810
BmiI GGNNCC 3 cut(s) 270, 530, 812
BmrFI CCNGG 3 cut(s) 590, 595, 740
BmrI ACTGGG 2 cut(s) 570, 809
BmuI ACTGGG 2 cut(s) 570, 809
BpiI GAAGAC 4 cut(s) 357, 417, 532, 638
Bpu14I TTCGAA 2 cut(s) 117, 360
Bsa29I ATCGAT 2 cut(s) 144, 786
BsaBI GATNNNNATC 1 cut(s) 832
BsaHI GRCGYC 5 cut(s) 5, 150, 156, 162, 654
BsaJI CCNNGG 3 cut(s) 588, 594, 739
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
Bsc4I CCNNNNNNNGG 3 cut(s) 594, 595, 819
Bse1I ACTGG 2 cut(s) 565, 815
Bse3DI GCAATG 1 cut(s) 582
Bse8I GATNNNNATC 1 cut(s) 832
BseBI CCWGG 3 cut(s) 590, 595, 740
BseCI ATCGAT 2 cut(s) 144, 786
BseDI CCNNGG 3 cut(s) 588, 594, 739
BseGI GGATG 2 cut(s) 48, 299
BseJI GATNNNNATC 1 cut(s) 832
BseLI CCNNNNNNNGG 3 cut(s) 594, 595, 819
BseMI GCAATG 1 cut(s) 582
BseNI ACTGG 2 cut(s) 565, 815
BseRI GAGGAG 5 cut(s) 17, 26, 79, 163, 433
BseXI GCAGC 2 cut(s) 403, 722
BseYI CCCAGC 1 cut(s) 381
Bsh1236I CGCG 3 cut(s) 22, 518, 719
BshFI GGCC 2 cut(s) 366, 593
BshVI ATCGAT 2 cut(s) 144, 786
BsiHKAI GWGCWC 1 cut(s) 765
BslFI GGGAC 3 cut(s) 359, 796, 835
BslI CCNNNNNNNGG 3 cut(s) 594, 595, 819
BsmAI GTCTC 2 cut(s) 654, 660
BsmBI CGTCTC 2 cut(s) 654, 660
BsmFI GGGAC 3 cut(s) 359, 796, 835
BsnI GGCC 2 cut(s) 366, 593
Bsp119I TTCGAA 2 cut(s) 117, 360
Bsp1286I GDGCHC 1 cut(s) 765
Bsp143I GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
BspANI GGCC 2 cut(s) 366, 593
BspDI ATCGAT 2 cut(s) 144, 786
BspFNI CGCG 3 cut(s) 22, 518, 719
BspLI GGNNCC 3 cut(s) 270, 530, 812
BspPI GGATC 5 cut(s) 263, 276, 449, 492, 782
BspT104I TTCGAA 2 cut(s) 117, 360
BsrBI CCGCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 582
BsrI ACTGG 2 cut(s) 565, 815
BssECI CCNNGG 3 cut(s) 588, 594, 739
BssMI GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
BssNI GRCGYC 5 cut(s) 5, 150, 156, 162, 654
Bst2UI CCWGG 3 cut(s) 590, 595, 740
Bst6I CTCTTC 3 cut(s) 50, 68, 189
BstACI GRCGYC 5 cut(s) 5, 150, 156, 162, 654
BstBI TTCGAA 2 cut(s) 117, 360
BstC8I GCNNGC 1 cut(s) 520
BstF5I GGATG 2 cut(s) 48, 299
BstFNI CGCG 3 cut(s) 22, 518, 719
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 9 cut(s) 271, 310, 457, 500, 516, 537, 786, 790, 830
BstMAI GTCTC 2 cut(s) 654, 660
BstMBI GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
BstMWI GCNNNNNNNGC 4 cut(s) 391, 400, 599, 608
BstNI CCWGG 3 cut(s) 590, 595, 740
BstSCI CCNGG 3 cut(s) 588, 593, 738
BstUI CGCG 3 cut(s) 22, 518, 719
BstV1I GCAGC 2 cut(s) 403, 722
BstV2I GAAGAC 4 cut(s) 357, 417, 532, 638
BstX2I RGATCY 2 cut(s) 268, 497
BstYI RGATCY 2 cut(s) 268, 497
Bsu15I ATCGAT 2 cut(s) 144, 786
BsuRI GGCC 2 cut(s) 366, 593
BsuTUI ATCGAT 2 cut(s) 144, 786
BtsCI GGATG 2 cut(s) 48, 299
BtsIMutI CAGTG 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 520
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 810
ClaI ATCGAT 2 cut(s) 144, 786
CseI GACGC 4 cut(s) 158, 164, 170, 643
Csp6I GTAC 2 cut(s) 257, 423
CviJI RGCY 7 cut(s) 333, 366, 481, 593, 745, 763, 771
CviKI_1 RGCY 7 cut(s) 333, 366, 481, 593, 745, 763, 771
CviQI GTAC 2 cut(s) 257, 423
DpnI GATC 9 cut(s) 270, 309, 456, 499, 515, 536, 785, 789, 829
DpnII GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
Eam1104I CTCTTC 3 cut(s) 50, 68, 189
EarI CTCTTC 3 cut(s) 50, 68, 189
EciI GGCGGA 2 cut(s) 457, 742
Ecl136II GAGCTC 1 cut(s) 763
Eco147I AGGCCT 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 810
Eco53kI GAGCTC 1 cut(s) 763
Eco57I CTGAAG 1 cut(s) 625
EcoICRI GAGCTC 1 cut(s) 763
EcoRII CCWGG 3 cut(s) 588, 593, 738
EcoT38I GRGCYC 1 cut(s) 765
Esp3I CGTCTC 2 cut(s) 654, 660
FaiI YATR 2 cut(s) 845, 847
FaqI GGGAC 3 cut(s) 359, 796, 835
FauI CCCGC 4 cut(s) 206, 285, 493, 561
Fnu4HI GCNGC 6 cut(s) 102, 392, 489, 600, 736, 772
FokI GGATG 2 cut(s) 35, 286
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 6 cut(s) 102, 392, 489, 600, 736, 772
GlaI GCGC 1 cut(s) 23
GluI GCNGC 6 cut(s) 102, 392, 489, 600, 736, 772
GsaI CCCAGC 1 cut(s) 385
HaeIII GGCC 2 cut(s) 366, 593
HgaI GACGC 4 cut(s) 158, 164, 170, 643
HhaI GCGC 1 cut(s) 24
Hin1I GRCGYC 5 cut(s) 5, 150, 156, 162, 654
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HinfI GANTC 7 cut(s) 107, 123, 170, 287, 293, 620, 671
HphI GGTGA 4 cut(s) 202, 234, 583, 666
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 5 cut(s) 292, 454, 495, 659, 699
Hpy188III TCNNGA 3 cut(s) 284, 532, 631
Hpy8I GTNNAC 1 cut(s) 574
Hpy99I CGWCG 7 cut(s) 131, 140, 158, 164, 170, 659, 757
HpyAV CCTTC 7 cut(s) 88, 113, 213, 330, 356, 682, 686
HpyCH4IV ACGT 1 cut(s) 702
HpyCH4V TGCA 1 cut(s) 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 391, 400, 599, 608
HpySE526I ACGT 1 cut(s) 702
Hsp92I GRCGYC 5 cut(s) 5, 150, 156, 162, 654
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
LmnI GCTCC 2 cut(s) 496, 760
Lsp1109I GCAGC 2 cut(s) 403, 722
MaeII ACGT 1 cut(s) 702
MaeIII GTNAC 1 cut(s) 6
MalI GATC 9 cut(s) 270, 309, 456, 499, 515, 536, 785, 789, 829
MbiI CCGCTC 1 cut(s) 491
MboI GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
MboII GAAGA 9 cut(s) 37, 55, 176, 302, 362, 422, 532, 638, 646
MflI RGATCY 2 cut(s) 268, 497
MhlI GDGCHC 1 cut(s) 765
MluCI AATT 3 cut(s) 113, 448, 605
MlyI GAGTC 4 cut(s) 101, 164, 614, 665
MmeI TCCRAC 2 cut(s) 400, 637
MroXI GAANNNNTTC 2 cut(s) 638, 704
MspR9I CCNGG 3 cut(s) 590, 595, 740
MvaI CCWGG 3 cut(s) 590, 595, 740
MvnI CGCG 3 cut(s) 22, 518, 719
MwoI GCNNNNNNNGC 4 cut(s) 391, 400, 599, 608
NdeII GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
NlaIV GGNNCC 3 cut(s) 270, 530, 812
NmuCI GTSAC 1 cut(s) 6
NspV TTCGAA 2 cut(s) 117, 360
PceI AGGCCT 1 cut(s) 366
PcsI WCGNNNNNNNCGW 7 cut(s) 123, 132, 135, 150, 156, 162, 783
PdmI GAANNNNTTC 2 cut(s) 638, 704
PfeI GAWTC 3 cut(s) 123, 287, 293
PkrI GCNGC 6 cut(s) 103, 393, 490, 601, 737, 773
PleI GAGTC 4 cut(s) 101, 164, 614, 665
PpsI GAGTC 4 cut(s) 101, 164, 614, 665
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 3 cut(s) 588, 593, 738
PspFI CCCAGC 1 cut(s) 381
PspGI CCWGG 3 cut(s) 588, 593, 738
PspN4I GGNNCC 3 cut(s) 270, 530, 812
PspPI GGNCC 1 cut(s) 810
PsrI GAACNNNNNNTAC 2 cut(s) 240, 272
PsuI RGATCY 2 cut(s) 268, 497
RsaI GTAC 2 cut(s) 258, 424
RsaNI GTAC 2 cut(s) 257, 423
SacI GAGCTC 1 cut(s) 765
SatI GCNGC 6 cut(s) 102, 392, 489, 600, 736, 772
Sau3AI GATC 9 cut(s) 268, 307, 454, 497, 513, 534, 783, 787, 827
Sau96I GGNCC 1 cut(s) 810
SchI GAGTC 4 cut(s) 101, 164, 614, 665
ScrFI CCNGG 3 cut(s) 590, 595, 740
SduI GDGCHC 1 cut(s) 765
SfuI TTCGAA 2 cut(s) 117, 360
SinI GGWCC 1 cut(s) 810
Sse9I AATT 3 cut(s) 113, 448, 605
SseBI AGGCCT 1 cut(s) 366
SstI GAGCTC 1 cut(s) 765
StuI AGGCCT 1 cut(s) 366
StyD4I CCNGG 3 cut(s) 588, 593, 738
TaiI ACGT 1 cut(s) 705
TasI AATT 3 cut(s) 113, 448, 605
TauI GCSGC 4 cut(s) 104, 491, 602, 774
TfiI GAWTC 3 cut(s) 123, 287, 293
TscAI CASTG 1 cut(s) 565
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 2 cut(s) 391, 735
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 2 cut(s) 37, 532
TspGWI ACGGA 4 cut(s) 82, 244, 281, 678
TspRI CASTG 1 cut(s) 565
VpaK11BI GGWCC 1 cut(s) 810
XapI RAATTY 1 cut(s) 605
XmnI GAANNNNTTC 2 cut(s) 638, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.