MD15G1434000.v1.1

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007803
Physical Location & Seq
Forward (+)
53432368 .. 53435184
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1434000.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGGCGATGGCTTCACTCTCTTACACGCGCCTCCTTTCTCTGCTCTCCCTCTTCATCCTCCTCTTCCTCTTCTCTCACATTTCCTTCTCTCCCTCCTCCGTCCTCGCCGACTCCCAATTCGAAGGCTTCGACGACGCCGACGAAATCGACGATACCGACGACGACGACTCCTCACTCCCTCTCCCCACTCGCACCCATCCTTCCCTCACCACCACCTCCCAACCCGACCCACCTCCCTCCACCGATCCCTCCCTAGCCGACCCGGATCCCAACCCGATTCCCGATTCGGATTCTCTATCCACCGATCTTCCGAAGCCTTCCTCCACCAGCTTTGAGGATTGGGATGAAGATGAGTTCGAAGGCCTACCCGTCCTACCGCAGCAGAAACCGCCCCTCCACGACCCTGCAATTACTGAAAATGCCACTCCCTCCTCCGATTCCGATCCCAAACCCTCCGCTGACCCCAAGCCCCCCGCCGCGCCGAGATCCTTTGTTGTGGAGATCGCGTGCGGAGGGTTCCTGATCGTCTTCATCATCAACTACTTCACCGGGAAGCGGGTGAACGAGAACATCGCCCTGGCTTGGGCGGCGAAATTCGCCACCAAAGACTCGATTTTCGAGAAGAACTTCAGTATTTTGGGCGTCGGAGATGGCGGCGCTGGTGACGATCCGCCCTTGCTGTTGAAGGAAGGGCAGAACGTGTTCAAGTTTTACGCGAGTGGGCGGAGGTGCTGCCAGGGGCTTCTGGCGACGATGGAGCTCAAGAGCCGCCACGATTTGATCTCCTCGATTTTCAACTTGGTGGTTCCCAGTAGGGACGAGATCAATATCGAGGTTTATATGAACGATGAAGCGATGGACCACGTGGTTTTTGCCTTGGCGAGGAAGAAGTCGGCCAAGGCAATGCAGAAGGAGGTTAGGGACTTGCAGAAGTTTGCCGGGATATTGTCTCCGCCTACTGGAAGGAAGTGGGTTTCTGAGGACCTGGCGGTTATCTCCGAATCGAAGGAAGTGGCTGGTGATTTGATCACTGAGGCTGTGCTCGAACAGGTTTTAGGTGAAAAGGCTTTTCAGAAATTTGGAAAGGGTTTCATCTACATGCATTTTTCAGATCAACATCCAGGCTCGCAAAAGAAGATGCTGTTGTTCAAGTTTGCCCTACCGGATGCTGGTAACATGGCTGATATGACTCGTCTGGTGGCGTTGGTACCTTATTATATTGATTTGATTGGGCGCTACAAGCTAAGCAGTCAGGCTCGATCCAAAACTGAAGCAATCAGATCAAAGGCGGCGCAAGAGGCTTACAAAGAACTCCAAAATGCAAGGCAAGAAGCATTGCAAAGAAAGAAGGCGGAGAGGAGAAAGATGATGGAGGAGGTCGAGGCTAAGCTCAGTGCAGAGAGTATTCGCAAGAAAGAAGCAAAAGAGCGTGCTCGTCAGATGAAGAAGGCAATGCCGAAGATAAAAATGACCCGTGGTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

495

Amino Acids

54.73

Weight (kDa)

5.44

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC47 PF07946 163 - 483 1.9e-84 PAT complex subunit CCDC47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1207
AccB1I GGYRCC 1 cut(s) 1207
AccII CGCG 4 cut(s) 28, 479, 506, 716
AclWI GGATC 7 cut(s) 239, 260, 273, 437, 480, 662, 1254
AcoI YGGCCR 1 cut(s) 894
AcsI RAATTY 2 cut(s) 593, 1076
AcuI CTGAAG 2 cut(s) 613, 1290
AcvI CACGTG 1 cut(s) 865
AcyI GRCGYC 2 cut(s) 135, 642
AfaI GTAC 1 cut(s) 1209
AfiI CCNNNNNNNGG 6 cut(s) 555, 582, 583, 882, 959, 1169
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 4 cut(s) 685, 706, 796, 1150
AjnI CCWGG 4 cut(s) 576, 735, 984, 1120
AluBI AGCT 5 cut(s) 330, 760, 1243, 1390, 1482
AluI AGCT 5 cut(s) 330, 760, 1243, 1390, 1482
Alw21I GWGCWC 3 cut(s) 762, 1044, 1435
Alw26I GTCTC 1 cut(s) 954
AlwI GGATC 7 cut(s) 239, 260, 273, 437, 480, 662, 1254
AoxI GGCC 2 cut(s) 361, 894
ApeKI GCWGC 2 cut(s) 379, 732
ApoI RAATTY 2 cut(s) 593, 1076
ArsI GACNNNNNNTTYG 4 cut(s) 101, 133, 599, 631
Asp700I GAANNNNTTC 2 cut(s) 626, 701
Asp718I GGTACC 1 cut(s) 1207
AspLEI GCGC 5 cut(s) 30, 481, 659, 1236, 1294
AspS9I GGNCC 2 cut(s) 859, 982
AsuC2I CCSGG 3 cut(s) 263, 550, 940
AsuHPI GGTGA 6 cut(s) 199, 538, 571, 674, 1031, 1070
AsuII TTCGAA 2 cut(s) 120, 357
AvaII GGWCC 2 cut(s) 859, 982
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 1 cut(s) 1207
BanII GRGCYC 1 cut(s) 762
BbrPI CACGTG 1 cut(s) 865
BbsI GAAGAC 1 cut(s) 520
Bbv12I GWGCWC 3 cut(s) 762, 1044, 1435
BbvI GCAGC 2 cut(s) 391, 719
BccI CCATC 5 cut(s) 204, 644, 748, 850, 1363
BciT130I CCWGG 4 cut(s) 578, 737, 986, 1122
BclI TGATCA 1 cut(s) 1026
BcnI CCSGG 3 cut(s) 263, 550, 940
BcoDI GTCTC 1 cut(s) 954
BfaI CTAG 1 cut(s) 254
BfoI RGCGCY 2 cut(s) 660, 1237
BisI GCNGC 7 cut(s) 380, 477, 588, 655, 733, 769, 1290
BlpI GCTNAGC 2 cut(s) 1244, 1386
BlsI GCNGC 7 cut(s) 381, 478, 589, 656, 734, 770, 1291
Bme1390I CCNGG 7 cut(s) 263, 550, 578, 737, 940, 986, 1122
Bme18I GGWCC 2 cut(s) 859, 982
BmgT120I GGNCC 2 cut(s) 859, 982
BmiI GGNNCC 4 cut(s) 267, 518, 807, 1209
BmrFI CCNGG 7 cut(s) 263, 550, 578, 737, 940, 986, 1122
BmrI ACTGGG 1 cut(s) 804
BmsI GCATC 2 cut(s) 1128, 1156
BmuI ACTGGG 1 cut(s) 804
BpiI GAAGAC 1 cut(s) 520
Bpu1102I GCTNAGC 2 cut(s) 1244, 1386
Bpu14I TTCGAA 2 cut(s) 120, 357
BpuEI CTTGAG 1 cut(s) 746
BpuMI CCSGG 3 cut(s) 263, 550, 940
BsaAI YACGTR 1 cut(s) 865
BsaBI GATNNNNATC 3 cut(s) 441, 827, 1116
BsaHI GRCGYC 2 cut(s) 135, 642
BsaJI CCNNGG 5 cut(s) 576, 736, 876, 897, 1474
BsaWI WCCGGW 1 cut(s) 1162
BsaXI ACNNNNNCTCC 8 cut(s) 152, 165, 182, 195, 378, 408, 491, 521
Bsc4I CCNNNNNNNGG 6 cut(s) 555, 582, 583, 882, 959, 1169
Bse1I ACTGG 2 cut(s) 810, 964
Bse3DI GCAATG 3 cut(s) 909, 1334, 1458
Bse8I GATNNNNATC 3 cut(s) 441, 827, 1116
BseBI CCWGG 4 cut(s) 578, 737, 986, 1122
BseDI CCNNGG 5 cut(s) 576, 736, 876, 897, 1474
BseGI GGATG 5 cut(s) 54, 196, 349, 1117, 1171
BseJI GATNNNNATC 3 cut(s) 441, 827, 1116
BseLI CCNNNNNNNGG 6 cut(s) 555, 582, 583, 882, 959, 1169
BseMI GCAATG 3 cut(s) 909, 1334, 1458
BseMII CTCAG 3 cut(s) 969, 1023, 1405
BseNI ACTGG 2 cut(s) 810, 964
BseRI GAGGAG 7 cut(s) 50, 85, 160, 421, 775, 1372, 1388
BseXI GCAGC 2 cut(s) 391, 719
BsgI GTGCAG 1 cut(s) 1416
Bsh1236I CGCG 4 cut(s) 28, 479, 506, 716
BshFI GGCC 2 cut(s) 363, 896
BshNI GGYRCC 1 cut(s) 1207
BsiHKAI GWGCWC 3 cut(s) 762, 1044, 1435
BsiSI CCGG 4 cut(s) 263, 549, 939, 1163
BslFI GGGAC 2 cut(s) 830, 935
BslI CCNNNNNNNGG 6 cut(s) 555, 582, 583, 882, 959, 1169
BsmAI GTCTC 1 cut(s) 954
BsmFI GGGAC 2 cut(s) 830, 935
BsnI GGCC 2 cut(s) 363, 896
Bsp119I TTCGAA 2 cut(s) 120, 357
Bsp1286I GDGCHC 3 cut(s) 762, 1044, 1435
Bsp1720I GCTNAGC 2 cut(s) 1244, 1386
BspANI GGCC 2 cut(s) 363, 896
BspCNI CTCAG 3 cut(s) 970, 1024, 1404
BspFNI CGCG 4 cut(s) 28, 479, 506, 716
BspLI GGNNCC 4 cut(s) 267, 518, 807, 1209
BspPI GGATC 7 cut(s) 239, 260, 273, 437, 480, 662, 1254
BspT104I TTCGAA 2 cut(s) 120, 357
BspT107I GGYRCC 1 cut(s) 1207
BsrDI GCAATG 3 cut(s) 909, 1334, 1458
BsrI ACTGG 2 cut(s) 810, 964
BssECI CCNNGG 5 cut(s) 576, 736, 876, 897, 1474
BssNI GRCGYC 2 cut(s) 135, 642
BssT1I CCWWGG 2 cut(s) 876, 897
Bst2UI CCWGG 4 cut(s) 578, 737, 986, 1122
Bst6I CTCTTC 3 cut(s) 56, 68, 74
BstACI GRCGYC 2 cut(s) 135, 642
BstBAI YACGTR 1 cut(s) 865
BstBI TTCGAA 2 cut(s) 120, 357
BstC8I GCNNGC 3 cut(s) 508, 1127, 1431
BstDEI CTNAG 5 cut(s) 978, 1032, 1244, 1386, 1391
BstDSI CCRYGG 1 cut(s) 1474
BstENI CCTNNNNNAGG 1 cut(s) 880
BstF5I GGATG 5 cut(s) 54, 196, 349, 1117, 1171
BstFNI CGCG 4 cut(s) 28, 479, 506, 716
BstH2I RGCGCY 2 cut(s) 660, 1237
BstHHI GCGC 5 cut(s) 30, 481, 659, 1236, 1294
BstMAI GTCTC 1 cut(s) 954
BstMWI GCNNNNNNNGC 5 cut(s) 388, 587, 596, 1240, 1298
BstNI CCWGG 4 cut(s) 578, 737, 986, 1122
BstNSI RCATGY 1 cut(s) 1102
BstSCI CCNGG 7 cut(s) 261, 548, 576, 735, 938, 984, 1120
BstUI CGCG 4 cut(s) 28, 479, 506, 716
BstV1I GCAGC 2 cut(s) 391, 719
BstV2I GAAGAC 1 cut(s) 520
BstX2I RGATCY 2 cut(s) 265, 485
BstYI RGATCY 2 cut(s) 265, 485
BsuRI GGCC 2 cut(s) 363, 896
BtgI CCRYGG 1 cut(s) 1474
BtgZI GCGATG 3 cut(s) 20, 556, 869
BtsCI GGATG 5 cut(s) 54, 196, 349, 1117, 1171
BtsIMutI CAGTG 2 cut(s) 1029, 1399
Cac8I GCNNGC 3 cut(s) 508, 1127, 1431
CfoI GCGC 5 cut(s) 30, 481, 659, 1236, 1294
Cfr13I GGNCC 2 cut(s) 859, 982
CseI GACGC 2 cut(s) 143, 631
Csp6I GTAC 1 cut(s) 1208
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 2 cut(s) 1099, 1177
CviQI GTAC 1 cut(s) 1208
DdeI CTNAG 5 cut(s) 978, 1032, 1244, 1386, 1391
EaeI YGGCCR 1 cut(s) 894
Eam1104I CTCTTC 3 cut(s) 56, 68, 74
EarI CTCTTC 3 cut(s) 56, 68, 74
EciI GGCGGA 4 cut(s) 660, 739, 942, 1367
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 876, 897
Eco147I AGGCCT 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 762
Eco47I GGWCC 2 cut(s) 859, 982
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 2 cut(s) 613, 1290
Eco72I CACGTG 1 cut(s) 865
EcoICRI GAGCTC 1 cut(s) 760
EcoNI CCTNNNNNAGG 1 cut(s) 880
EcoO109I RGGNCCY 1 cut(s) 982
EcoRII CCWGG 4 cut(s) 576, 735, 984, 1120
EcoT14I CCWWGG 2 cut(s) 876, 897
EcoT22I ATGCAT 1 cut(s) 1104
EcoT38I GRGCYC 1 cut(s) 762
ErhI CCWWGG 2 cut(s) 876, 897
FaeI CATG 2 cut(s) 1102, 1180
FaiI YATR 6 cut(s) 840, 842, 1100, 1178, 1187, 1218
FaqI GGGAC 2 cut(s) 830, 935
FatI CATG 2 cut(s) 1098, 1176
FauI CCCGC 2 cut(s) 481, 549
FbaI TGATCA 1 cut(s) 1026
Fnu4HI GCNGC 7 cut(s) 380, 477, 588, 655, 733, 769, 1290
FokI GGATG 5 cut(s) 41, 183, 356, 1104, 1178
FriOI GRGCYC 1 cut(s) 762
Fsp4HI GCNGC 7 cut(s) 380, 477, 588, 655, 733, 769, 1290
FspBI CTAG 1 cut(s) 254
GlaI GCGC 5 cut(s) 29, 480, 658, 1235, 1293
GluI GCNGC 7 cut(s) 380, 477, 588, 655, 733, 769, 1290
HaeII RGCGCY 2 cut(s) 660, 1237
HaeIII GGCC 2 cut(s) 363, 896
HapII CCGG 4 cut(s) 263, 549, 939, 1163
HgaI GACGC 2 cut(s) 143, 631
HhaI GCGC 5 cut(s) 30, 481, 659, 1236, 1294
Hin1I GRCGYC 2 cut(s) 135, 642
Hin1II CATG 2 cut(s) 1102, 1180
Hin6I GCGC 5 cut(s) 28, 479, 657, 1234, 1292
HinP1I GCGC 5 cut(s) 28, 479, 657, 1234, 1292
HinfI GANTC 9 cut(s) 110, 167, 277, 284, 290, 437, 608, 1001, 1189
HpaII CCGG 4 cut(s) 263, 549, 939, 1163
HphI GGTGA 6 cut(s) 199, 538, 571, 674, 1031, 1070
Hpy166II GTNNAC 1 cut(s) 562
Hpy188III TCNNGA 4 cut(s) 281, 520, 619, 763
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 9 cut(s) 134, 137, 143, 152, 161, 164, 167, 647, 754
HpyCH4IV ACGT 2 cut(s) 699, 864
HpyCH4V TGCA 7 cut(s) 407, 907, 928, 1102, 1322, 1339, 1397
HpyF10VI GCNNNNNNNGC 5 cut(s) 388, 587, 596, 1240, 1298
HpyF3I CTNAG 5 cut(s) 978, 1032, 1244, 1386, 1391
HpySE526I ACGT 2 cut(s) 699, 864
Hsp92I GRCGYC 2 cut(s) 135, 642
Hsp92II CATG 2 cut(s) 1102, 1180
HspAI GCGC 5 cut(s) 28, 479, 657, 1234, 1292
KpnI GGTACC 1 cut(s) 1211
Ksp22I TGATCA 1 cut(s) 1026
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 2 cut(s) 391, 719
LweI GCATC 2 cut(s) 1128, 1156
MaeI CTAG 1 cut(s) 254
MaeII ACGT 2 cut(s) 699, 864
MaeIII GTNAC 2 cut(s) 662, 1172
MflI RGATCY 2 cut(s) 265, 485
MhlI GDGCHC 3 cut(s) 762, 1044, 1435
MluCI AATT 4 cut(s) 116, 408, 593, 1076
MlyI GAGTC 4 cut(s) 104, 161, 602, 1183
MmeI TCCRAC 1 cut(s) 625
Mph1103I ATGCAT 1 cut(s) 1104
MroXI GAANNNNTTC 2 cut(s) 626, 701
MslI CAYNNNNRTG 1 cut(s) 1097
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 4 cut(s) 263, 549, 939, 1163
MspR9I CCNGG 7 cut(s) 263, 550, 578, 737, 940, 986, 1122
MvaI CCWGG 4 cut(s) 578, 737, 986, 1122
MvnI CGCG 4 cut(s) 28, 479, 506, 716
MwoI GCNNNNNNNGC 5 cut(s) 388, 587, 596, 1240, 1298
NciI CCSGG 3 cut(s) 263, 550, 940
NlaIII CATG 2 cut(s) 1102, 1180
NlaIV GGNNCC 4 cut(s) 267, 518, 807, 1209
NmeAIII GCCGAG 1 cut(s) 507
NmuCI GTSAC 1 cut(s) 662
NsiI ATGCAT 1 cut(s) 1104
NspI RCATGY 1 cut(s) 1102
NspV TTCGAA 2 cut(s) 120, 357
PceI AGGCCT 1 cut(s) 363
PcsI WCGNNNNNNNCGW 6 cut(s) 126, 135, 138, 147, 153, 156
PdmI GAANNNNTTC 2 cut(s) 626, 701
PfeI GAWTC 5 cut(s) 277, 284, 290, 437, 1001
PkrI GCNGC 7 cut(s) 381, 478, 589, 656, 734, 770, 1291
PleI GAGTC 4 cut(s) 104, 161, 602, 1183
PmaCI CACGTG 1 cut(s) 865
PmlI CACGTG 1 cut(s) 865
PpsI GAGTC 4 cut(s) 104, 161, 602, 1183
Ppu21I YACGTR 1 cut(s) 865
PpuMI RGGWCCY 1 cut(s) 982
Psp124BI GAGCTC 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 982
Psp6I CCWGG 4 cut(s) 576, 735, 984, 1120
PspCI CACGTG 1 cut(s) 865
PspGI CCWGG 4 cut(s) 576, 735, 984, 1120
PspN4I GGNNCC 4 cut(s) 267, 518, 807, 1209
PspPI GGNCC 2 cut(s) 859, 982
PspPPI RGGWCCY 1 cut(s) 982
PsuI RGATCY 2 cut(s) 265, 485
RsaI GTAC 1 cut(s) 1209
RsaNI GTAC 1 cut(s) 1208
RseI CAYNNNNRTG 1 cut(s) 1097
SacI GAGCTC 1 cut(s) 762
SatI GCNGC 7 cut(s) 380, 477, 588, 655, 733, 769, 1290
Sau96I GGNCC 2 cut(s) 859, 982
SchI GAGTC 4 cut(s) 104, 161, 602, 1183
ScrFI CCNGG 7 cut(s) 263, 550, 578, 737, 940, 986, 1122
SduI GDGCHC 3 cut(s) 762, 1044, 1435
SfaNI GCATC 2 cut(s) 1128, 1156
SfuI TTCGAA 2 cut(s) 120, 357
SinI GGWCC 2 cut(s) 859, 982
SmiMI CAYNNNNRTG 1 cut(s) 1097
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 4 cut(s) 116, 408, 593, 1076
SseBI AGGCCT 1 cut(s) 363
SspMI CTAG 1 cut(s) 254
SstI GAGCTC 1 cut(s) 762
StuI AGGCCT 1 cut(s) 363
StyD4I CCNGG 7 cut(s) 261, 548, 576, 735, 938, 984, 1120
StyI CCWWGG 2 cut(s) 876, 897
TaiI ACGT 2 cut(s) 702, 867
TasI AATT 4 cut(s) 116, 408, 593, 1076
TauI GCSGC 5 cut(s) 479, 590, 657, 771, 1292
TfiI GAWTC 5 cut(s) 277, 284, 290, 437, 1001
TscAI CASTG 2 cut(s) 1036, 1399
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 2 cut(s) 379, 732
Tsp45I GTSAC 1 cut(s) 662
TspDTI ATGAA 7 cut(s) 43, 360, 520, 857, 864, 1081, 1457
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 2 cut(s) 1036, 1399
VpaK11BI GGWCC 2 cut(s) 859, 982
XagI CCTNNNNNAGG 1 cut(s) 880
XapI RAATTY 2 cut(s) 593, 1076
XceI RCATGY 1 cut(s) 1102
XmnI GAANNNNTTC 2 cut(s) 626, 701
XspI CTAG 1 cut(s) 254
Zsp2I ATGCAT 1 cut(s) 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.