pycom08g20870

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007803
Physical Location & Seq
Forward (+)
20493508 .. 20495459
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20870.3

Sequence Viewer

Length: 582 bp
ATGCAGAAGGAGGTTGGGGACTTGCAGAAGTTTGCCGGGATAGTGTCTCCGCCTACCGGAAGGAAGTGGGTTTCTGAGGACCTGGCGGTTATCTCCGAATCGAAGGAAGTGGCTGGTGATTTGATCACTGAGGCTGTGCTCGAACAGGTTTTTGGTGAAAAGGCTTTTCAGAAATTTGGAAAGGGTTTCATCTACATGCATTTTTCAGATCAACATCCAGGCTCGCGAAAGAAGATGCTGTTGTTCAAGTTTGCCCTACCAGATGCCGGTAACATGGCTGATATGACCCGACTGGTGGCTTTGGTACCTTATTATATTGATTTGATTGGGCGCTACAAGCTAAGTAGTCAGGTTCGATCCAAAACTGAAGCAATCAGATCAAAGGCGGTTCAAGAGGCTTACAAAGAACTCCAAAATGCTAGGCAAGAAGCCTTGCAAAGAAAGAAGGCAGAGCGGAAAAAGATGATGGAGGAGGCCGAGGCTAAGCTCAGTGCAGATAGTATTCGCAAGAGAGAAGCAAAAGAGCGTGCTCGTCAGATGAAGAAGGCAATGCCGAAAGTAAAAATGACCCGTGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

194

Amino Acids

21.96

Weight (kDa)

9.88

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 304
AccB1I GGYRCC 1 cut(s) 304
AccBSI CCGCTC 1 cut(s) 454
AccII CGCG 1 cut(s) 226
AciI CCGC 4 cut(s) 50, 86, 386, 454
AclWI GGATC 1 cut(s) 351
AcsI RAATTY 1 cut(s) 173
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 306
AfiI CCNNNNNNNGG 3 cut(s) 56, 266, 295
AgsI TTSAA 2 cut(s) 247, 392
AjnI CCWGG 2 cut(s) 81, 217
AjuI GAANNNNNNNTTGG 2 cut(s) 135, 167
AluBI AGCT 3 cut(s) 340, 487, 579
AluI AGCT 3 cut(s) 340, 487, 579
Alw21I GWGCWC 2 cut(s) 141, 532
Alw26I GTCTC 1 cut(s) 51
AlwI GGATC 1 cut(s) 351
AoxI GGCC 1 cut(s) 474
ApoI RAATTY 1 cut(s) 173
Asp718I GGTACC 1 cut(s) 304
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 2 cut(s) 128, 167
AsuNHI GCTAGC 1 cut(s) 575
AvaII GGWCC 1 cut(s) 79
BanI GGYRCC 1 cut(s) 304
Bbv12I GWGCWC 2 cut(s) 141, 532
BccI CCATC 1 cut(s) 460
BciT130I CCWGG 2 cut(s) 83, 219
BclI TGATCA 1 cut(s) 123
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 3 cut(s) 420, 576, 580
BfoI RGCGCY 1 cut(s) 334
BlpI GCTNAGC 1 cut(s) 483
Bme1390I CCNGG 3 cut(s) 37, 83, 219
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 3 cut(s) 37, 83, 219
BmsI GCATC 2 cut(s) 225, 253
BmtI GCTAGC 1 cut(s) 579
Bpu1102I GCTNAGC 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 477
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 266, 295
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 555
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 2 cut(s) 83, 219
BseDI CCNNGG 1 cut(s) 477
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 3 cut(s) 56, 266, 295
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 3 cut(s) 66, 120, 502
BseNI ACTGG 1 cut(s) 297
BseRI GAGGAG 1 cut(s) 485
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 1 cut(s) 226
BshFI GGCC 1 cut(s) 476
BshNI GGYRCC 1 cut(s) 304
BsiHKAI GWGCWC 2 cut(s) 141, 532
BsiSI CCGG 3 cut(s) 36, 57, 267
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 3 cut(s) 56, 266, 295
BsmAI GTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 1 cut(s) 476
Bsp1286I GDGCHC 2 cut(s) 141, 532
Bsp143I GATC 4 cut(s) 123, 208, 356, 377
Bsp1720I GCTNAGC 1 cut(s) 483
Bsp68I TCGCGA 1 cut(s) 226
BspACI CCGC 4 cut(s) 50, 86, 386, 454
BspANI GGCC 1 cut(s) 476
BspCNI CTCAG 3 cut(s) 67, 121, 501
BspFNI CGCG 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 306
BspOI GCTAGC 1 cut(s) 579
BspPI GGATC 1 cut(s) 351
BspT107I GGYRCC 1 cut(s) 304
BsrBI CCGCTC 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 555
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 4 cut(s) 123, 208, 356, 377
Bst2UI CCWGG 2 cut(s) 83, 219
BstC8I GCNNGC 3 cut(s) 224, 528, 577
BstDEI CTNAG 5 cut(s) 75, 129, 341, 483, 488
BstF5I GGATG 1 cut(s) 214
BstFNI CGCG 1 cut(s) 226
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 1 cut(s) 333
BstKTI GATC 4 cut(s) 126, 211, 359, 380
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 4 cut(s) 123, 208, 356, 377
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstNI CCWGG 2 cut(s) 83, 219
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 3 cut(s) 35, 81, 217
BstUI CGCG 1 cut(s) 226
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 126, 496
BtuMI TCGCGA 1 cut(s) 226
Cac8I GCNNGC 3 cut(s) 224, 528, 577
CfoI GCGC 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 79
Csp6I GTAC 1 cut(s) 305
CviAII CATG 2 cut(s) 196, 274
CviQI GTAC 1 cut(s) 305
DdeI CTNAG 5 cut(s) 75, 129, 341, 483, 488
DpnI GATC 4 cut(s) 125, 210, 358, 379
DpnII GATC 4 cut(s) 123, 208, 356, 377
EciI GGCGGA 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 2 cut(s) 81, 217
EcoT22I ATGCAT 1 cut(s) 201
FaeI CATG 2 cut(s) 199, 277
FaiI YATR 4 cut(s) 197, 275, 284, 315
FaqI GGGAC 1 cut(s) 32
FatI CATG 2 cut(s) 195, 273
FbaI TGATCA 1 cut(s) 123
FokI GGATG 1 cut(s) 201
FspBI CTAG 3 cut(s) 420, 576, 580
GlaI GCGC 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 1 cut(s) 476
HapII CCGG 3 cut(s) 36, 57, 267
HhaI GCGC 1 cut(s) 333
Hin1II CATG 2 cut(s) 199, 277
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HinfI GANTC 1 cut(s) 98
HpaII CCGG 3 cut(s) 36, 57, 267
HphI GGTGA 2 cut(s) 128, 167
Hpy188I TCNGA 6 cut(s) 76, 97, 171, 208, 377, 537
Hpy188III TCNNGA 2 cut(s) 225, 392
HpyAV CCTTC 4 cut(s) 54, 97, 439, 538
HpyCH4V TGCA 5 cut(s) 4, 25, 199, 436, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpyF3I CTNAG 5 cut(s) 75, 129, 341, 483, 488
Hsp92II CATG 2 cut(s) 199, 277
HspAI GCGC 1 cut(s) 331
KpnI GGTACC 1 cut(s) 308
Ksp22I TGATCA 1 cut(s) 123
Kzo9I GATC 4 cut(s) 123, 208, 356, 377
LweI GCATC 2 cut(s) 225, 253
MaeI CTAG 3 cut(s) 420, 576, 580
MaeIII GTNAC 1 cut(s) 269
MalI GATC 4 cut(s) 125, 210, 358, 379
MbiI CCGCTC 1 cut(s) 454
MboI GATC 4 cut(s) 123, 208, 356, 377
MboII GAAGA 2 cut(s) 244, 553
MhlI GDGCHC 2 cut(s) 141, 532
MluCI AATT 1 cut(s) 173
MnlI CCTC 7 cut(s) 4, 70, 124, 388, 463, 466, 472
Mph1103I ATGCAT 1 cut(s) 201
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 3 cut(s) 36, 57, 267
MspR9I CCNGG 3 cut(s) 37, 83, 219
MvaI CCWGG 2 cut(s) 83, 219
MvnI CGCG 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 337
NciI CCSGG 1 cut(s) 37
NdeII GATC 4 cut(s) 123, 208, 356, 377
NheI GCTAGC 1 cut(s) 575
NlaIII CATG 2 cut(s) 199, 277
NlaIV GGNNCC 1 cut(s) 306
NmeAIII GCCGAG 1 cut(s) 502
NruI TCGCGA 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 201
NspI RCATGY 1 cut(s) 199
PfeI GAWTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 79
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 81, 217
PspGI CCWGG 2 cut(s) 81, 217
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 1 cut(s) 79
PspPPI RGGWCCY 1 cut(s) 79
RruI TCGCGA 1 cut(s) 226
RsaI GTAC 1 cut(s) 306
RsaNI GTAC 1 cut(s) 305
RseI CAYNNNNRTG 1 cut(s) 194
Sau3AI GATC 4 cut(s) 123, 208, 356, 377
Sau96I GGNCC 1 cut(s) 79
ScrFI CCNGG 3 cut(s) 37, 83, 219
SduI GDGCHC 2 cut(s) 141, 532
SetI ASST 8 cut(s) 15, 84, 150, 310, 342, 354, 489, 581
SfaNI GCATC 2 cut(s) 225, 253
SinI GGWCC 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 194
Sse9I AATT 1 cut(s) 173
SsiI CCGC 4 cut(s) 50, 86, 386, 454
SspMI CTAG 3 cut(s) 420, 576, 580
StyD4I CCNGG 3 cut(s) 35, 81, 217
TaqI TCGA 3 cut(s) 101, 141, 355
TasI AATT 1 cut(s) 173
TfiI GAWTC 1 cut(s) 98
TscAI CASTG 2 cut(s) 133, 496
TspDTI ATGAA 2 cut(s) 178, 554
TspRI CASTG 2 cut(s) 133, 496
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 1 cut(s) 173
XceI RCATGY 1 cut(s) 199
XspI CTAG 3 cut(s) 420, 576, 580
Zsp2I ATGCAT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.