pycom15g38300

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007803
Physical Location & Seq
Forward (+)
38130022 .. 38130997
976 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g38300.1

Sequence Viewer

Length: 936 bp
ATGTTGCCCGGTTGGTCACACCCTCGTATCAATCTCCCACATTCATTTAATACACTTTCTCGTTTCCTGTACGCTCTCTCTGTAGCAGGGGCAGTAAGCATGGTCATGGCGTCACTCTCTTACACGCGCCTCCTTTCTCTGCTCTCCCTCTTCATCCTCCTCTTCCTCTTCTCCCACCTTTCCTTCTCTCCCTCCTCCGTCCTCGCTGACTCCCAATTCGAAGGCTTCGACGACGCCGACGAAATCGACGATACCGACGACGACGACTCCTCACTTCCTCTCCCCACTCGCACCCGTCCTTCCCTCACCACCACCTCCCAACCCGACCCACCTACCTCCACCGATCCCTCCCTACCCGACCCGGATCCCAACCCGATTCCCGATTCGGATTCTCCATCCACCGATCTTCCGAAGCCTTCCTCCACCAGCTTCGAGGATTGGGATGAAGATGAGTTCGAAGGCCTACCCGTCCAACCGCAGCAGAAACCGCCCCTCCACGACCCTGCAATTACTGAAAATGCCACTCCCTCCTCCGATTCCGATCCCAAACCCTCCGCTGACCCCAAGCCCCCCGCCGCGCCGAGATCCTTTGTTGTGGAGATCGCGTGCGGAGGGTTCCTGATCGTCTTCATCATCAACTATTTCACCGGGAAGCGGGTGAACGAGAACATCGCCCTGGCTTGGGCGGCGAAATTCGCCACCAAAGACTCGATTTTCGAGAAGAACTTCAGTATTTTGGGCGTCGGAGACGGCGACGATTCGCCCTTGCTGTTGAAGGAAGGGCAGAACGTGTTCAAGTTTTACGCGAGTGGGCGGAGGTGCTGCCAGGGACTTCTGGCGACGATGGAGCTCAAGAGCCGCCACGATTTGATCGATCCTCGATTTTCAACTTGGTGGTTCCCAGTAGGGACGAGATCAATATCGAGGTTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

312

Amino Acids

34.2

Weight (kDa)

4.52

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC47 PF07946 196 - 291 1.6e-28 PAT complex subunit CCDC47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 127, 578, 605, 806
AclWI GGATC 6 cut(s) 338, 359, 372, 536, 579, 869
AcsI RAATTY 1 cut(s) 692
AcuI CTGAAG 1 cut(s) 712
AcyI GRCGYC 3 cut(s) 110, 234, 741
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 3 cut(s) 654, 681, 682
AflIII ACRYGT 1 cut(s) 789
AgsI TTSAA 3 cut(s) 775, 796, 888
AjnI CCWGG 2 cut(s) 675, 825
AluBI AGCT 2 cut(s) 429, 850
AluI AGCT 2 cut(s) 429, 850
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 1 cut(s) 741
AlwI GGATC 6 cut(s) 338, 359, 372, 536, 579, 869
AoxI GGCC 1 cut(s) 460
ApeKI GCWGC 2 cut(s) 478, 822
ApoI RAATTY 1 cut(s) 692
ArsI GACNNNNNNTTYG 4 cut(s) 200, 232, 698, 730
Asp700I GAANNNNTTC 2 cut(s) 725, 791
AspLEI GCGC 2 cut(s) 129, 580
AsuC2I CCSGG 3 cut(s) 9, 362, 649
AsuHPI GGTGA 3 cut(s) 298, 637, 670
AsuII TTCGAA 2 cut(s) 219, 456
BaeI ACNNNNGTAYC 2 cut(s) 10, 43
BamHI GGATCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 619
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 2 cut(s) 490, 809
BccI CCATC 2 cut(s) 403, 838
BceAI ACGGC 1 cut(s) 766
BciT130I CCWGG 2 cut(s) 677, 827
BcnI CCSGG 3 cut(s) 9, 362, 649
BcoDI GTCTC 1 cut(s) 741
BfmI CTRYAG 1 cut(s) 81
BisI GCNGC 5 cut(s) 479, 576, 687, 823, 859
BlsI GCNGC 5 cut(s) 480, 577, 688, 824, 860
Bme1390I CCNGG 5 cut(s) 9, 362, 649, 677, 827
BmiI GGNNCC 3 cut(s) 366, 617, 899
BmrFI CCNGG 5 cut(s) 9, 362, 649, 677, 827
BmrI ACTGGG 1 cut(s) 896
BmuI ACTGGG 1 cut(s) 896
BpiI GAAGAC 1 cut(s) 619
Bpu14I TTCGAA 2 cut(s) 219, 456
BpuEI CTTGAG 1 cut(s) 836
BpuMI CCSGG 3 cut(s) 9, 362, 649
Bsa29I ATCGAT 1 cut(s) 873
BsaBI GATNNNNATC 2 cut(s) 540, 919
BsaHI GRCGYC 3 cut(s) 110, 234, 741
BsaJI CCNNGG 2 cut(s) 675, 826
BsaXI ACNNNNNCTCC 8 cut(s) 251, 264, 281, 294, 477, 507, 590, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 654, 681, 682
Bse1I ACTGG 1 cut(s) 902
Bse8I GATNNNNATC 2 cut(s) 540, 919
BseBI CCWGG 2 cut(s) 677, 827
BseCI ATCGAT 1 cut(s) 873
BseDI CCNNGG 2 cut(s) 675, 826
BseGI GGATG 3 cut(s) 153, 395, 448
BseJI GATNNNNATC 2 cut(s) 540, 919
BseLI CCNNNNNNNGG 3 cut(s) 654, 681, 682
BseNI ACTGG 1 cut(s) 902
BseRI GAGGAG 4 cut(s) 149, 184, 259, 520
BseXI GCAGC 2 cut(s) 490, 809
Bsh1236I CGCG 4 cut(s) 127, 578, 605, 806
BshFI GGCC 1 cut(s) 462
BshVI ATCGAT 1 cut(s) 873
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 3 cut(s) 9, 362, 648
BslFI GGGAC 2 cut(s) 843, 922
BslI CCNNNNNNNGG 3 cut(s) 654, 681, 682
BsmAI GTCTC 1 cut(s) 741
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 2 cut(s) 843, 922
BsnI GGCC 1 cut(s) 462
Bsp119I TTCGAA 2 cut(s) 219, 456
Bsp1286I GDGCHC 1 cut(s) 852
BspANI GGCC 1 cut(s) 462
BspDI ATCGAT 1 cut(s) 873
BspFNI CGCG 4 cut(s) 127, 578, 605, 806
BspLI GGNNCC 3 cut(s) 366, 617, 899
BspPI GGATC 6 cut(s) 338, 359, 372, 536, 579, 869
BspT104I TTCGAA 2 cut(s) 219, 456
BsrI ACTGG 1 cut(s) 902
BssECI CCNNGG 2 cut(s) 675, 826
BssNI GRCGYC 3 cut(s) 110, 234, 741
Bst2UI CCWGG 2 cut(s) 677, 827
Bst6I CTCTTC 3 cut(s) 155, 167, 173
BstACI GRCGYC 3 cut(s) 110, 234, 741
BstBI TTCGAA 2 cut(s) 219, 456
BstC8I GCNNGC 1 cut(s) 607
BstF5I GGATG 3 cut(s) 153, 395, 448
BstFNI CGCG 4 cut(s) 127, 578, 605, 806
BstHHI GCGC 2 cut(s) 129, 580
BstMAI GTCTC 1 cut(s) 741
BstMWI GCNNNNNNNGC 3 cut(s) 487, 686, 695
BstNI CCWGG 2 cut(s) 677, 827
BstSCI CCNGG 5 cut(s) 7, 360, 647, 675, 825
BstSFI CTRYAG 1 cut(s) 81
BstUI CGCG 4 cut(s) 127, 578, 605, 806
BstV1I GCAGC 2 cut(s) 490, 809
BstV2I GAAGAC 1 cut(s) 619
BstX2I RGATCY 2 cut(s) 364, 584
BstYI RGATCY 2 cut(s) 364, 584
Bsu15I ATCGAT 1 cut(s) 873
BsuRI GGCC 1 cut(s) 462
BsuTUI ATCGAT 1 cut(s) 873
BtgZI GCGATG 1 cut(s) 655
BtsCI GGATG 3 cut(s) 153, 395, 448
Cac8I GCNNGC 1 cut(s) 607
CfoI GCGC 2 cut(s) 129, 580
ClaI ATCGAT 1 cut(s) 873
CseI GACGC 3 cut(s) 99, 242, 730
Csp6I GTAC 1 cut(s) 70
CviAII CATG 2 cut(s) 100, 106
CviJI RGCY 8 cut(s) 225, 415, 429, 462, 568, 680, 850, 858
CviKI_1 RGCY 8 cut(s) 225, 415, 429, 462, 568, 680, 850, 858
CviQI GTAC 1 cut(s) 70
Eam1104I CTCTTC 3 cut(s) 155, 167, 173
EarI CTCTTC 3 cut(s) 155, 167, 173
EciI GGCGGA 1 cut(s) 829
Ecl136II GAGCTC 1 cut(s) 850
Eco147I AGGCCT 1 cut(s) 462
Eco24I GRGCYC 1 cut(s) 852
Eco53kI GAGCTC 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 712
EcoICRI GAGCTC 1 cut(s) 850
EcoRII CCWGG 2 cut(s) 675, 825
EcoT38I GRGCYC 1 cut(s) 852
Esp3I CGTCTC 1 cut(s) 741
FaeI CATG 2 cut(s) 103, 109
FaiI YATR 4 cut(s) 101, 107, 932, 934
FaqI GGGAC 2 cut(s) 843, 922
FatI CATG 2 cut(s) 99, 105
FauI CCCGC 2 cut(s) 580, 648
Fnu4HI GCNGC 5 cut(s) 479, 576, 687, 823, 859
FokI GGATG 3 cut(s) 140, 382, 455
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 5 cut(s) 479, 576, 687, 823, 859
GlaI GCGC 2 cut(s) 128, 579
GluI GCNGC 5 cut(s) 479, 576, 687, 823, 859
HaeIII GGCC 1 cut(s) 462
HapII CCGG 3 cut(s) 9, 362, 648
HgaI GACGC 3 cut(s) 99, 242, 730
HhaI GCGC 2 cut(s) 129, 580
Hin1I GRCGYC 3 cut(s) 110, 234, 741
Hin1II CATG 2 cut(s) 103, 109
Hin6I GCGC 2 cut(s) 127, 578
HinP1I GCGC 2 cut(s) 127, 578
HinfI GANTC 8 cut(s) 209, 266, 376, 383, 389, 536, 707, 758
HpaII CCGG 3 cut(s) 9, 362, 648
HphI GGTGA 3 cut(s) 298, 637, 670
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 5 cut(s) 388, 411, 535, 541, 746
Hpy188III TCNNGA 4 cut(s) 380, 619, 718, 853
Hpy8I GTNNAC 1 cut(s) 661
HpyAV CCTTC 7 cut(s) 193, 215, 309, 426, 452, 769, 773
HpyCH4IV ACGT 1 cut(s) 789
HpyCH4V TGCA 1 cut(s) 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 487, 686, 695
HpySE526I ACGT 1 cut(s) 789
Hsp92I GRCGYC 3 cut(s) 110, 234, 741
Hsp92II CATG 2 cut(s) 103, 109
HspAI GCGC 2 cut(s) 127, 578
LmnI GCTCC 1 cut(s) 847
Lsp1109I GCAGC 2 cut(s) 490, 809
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 2 cut(s) 15, 111
MboII GAAGA 7 cut(s) 142, 154, 160, 398, 458, 619, 733
MflI RGATCY 2 cut(s) 364, 584
MhlI GDGCHC 1 cut(s) 852
MluCI AATT 3 cut(s) 215, 507, 692
MlyI GAGTC 3 cut(s) 203, 260, 701
MmeI TCCRAC 2 cut(s) 496, 724
MroXI GAANNNNTTC 2 cut(s) 725, 791
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 3 cut(s) 9, 362, 648
MspR9I CCNGG 5 cut(s) 9, 362, 649, 677, 827
MvaI CCWGG 2 cut(s) 677, 827
MvnI CGCG 4 cut(s) 127, 578, 605, 806
MwoI GCNNNNNNNGC 3 cut(s) 487, 686, 695
NciI CCSGG 3 cut(s) 9, 362, 649
NlaIII CATG 2 cut(s) 103, 109
NlaIV GGNNCC 3 cut(s) 366, 617, 899
NmeAIII GCCGAG 1 cut(s) 606
NmuCI GTSAC 2 cut(s) 15, 111
NspV TTCGAA 2 cut(s) 219, 456
PceI AGGCCT 1 cut(s) 462
PcsI WCGNNNNNNNCGW 8 cut(s) 225, 234, 237, 246, 252, 255, 750, 870
PdmI GAANNNNTTC 2 cut(s) 725, 791
PfeI GAWTC 5 cut(s) 376, 383, 389, 536, 758
PkrI GCNGC 5 cut(s) 480, 577, 688, 824, 860
PleI GAGTC 3 cut(s) 203, 260, 701
PpsI GAGTC 3 cut(s) 203, 260, 701
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 675, 825
PspGI CCWGG 2 cut(s) 675, 825
PspN4I GGNNCC 3 cut(s) 366, 617, 899
PsuI RGATCY 2 cut(s) 364, 584
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
RseI CAYNNNNRTG 1 cut(s) 104
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 5 cut(s) 479, 576, 687, 823, 859
SchI GAGTC 3 cut(s) 203, 260, 701
ScrFI CCNGG 5 cut(s) 9, 362, 649, 677, 827
SduI GDGCHC 1 cut(s) 852
SetI ASST 9 cut(s) 180, 317, 334, 338, 431, 792, 821, 852, 929
SfcI CTRYAG 1 cut(s) 81
SfuI TTCGAA 2 cut(s) 219, 456
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
Sse9I AATT 3 cut(s) 215, 507, 692
SseBI AGGCCT 1 cut(s) 462
SstI GAGCTC 1 cut(s) 852
StuI AGGCCT 1 cut(s) 462
StyD4I CCNGG 5 cut(s) 7, 360, 647, 675, 825
TaiI ACGT 1 cut(s) 792
TasI AATT 3 cut(s) 215, 507, 692
TauI GCSGC 3 cut(s) 578, 689, 861
TfiI GAWTC 5 cut(s) 376, 383, 389, 536, 758
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TseFI GTSAC 2 cut(s) 15, 111
TseI GCWGC 2 cut(s) 478, 822
Tsp45I GTSAC 2 cut(s) 15, 111
TspDTI ATGAA 4 cut(s) 33, 142, 459, 619
TspGWI ACGGA 1 cut(s) 187
XapI RAATTY 1 cut(s) 692
XmnI GAANNNNTTC 2 cut(s) 725, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.