Rh7CG355300

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0007803
Physical Location & Seq
Forward (+)
40763232 .. 40768749
5518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG355300.1

Sequence Viewer

Length: 1458 bp
ATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAGCGGAATCCCCCACCATCATCAAATATTAATTTGATTCCTTTCGTTACTCTACATAGAAGAGAAGAGCGACACTTCCAAACTTCCTTGTTGCAGCAGCAGCAGCAGCGGCGGCGTCGCATCATCATCATCATCATCACAATCATGGGTCTCGCTGTCCCACATCTCCTCACTCTCTTCCTCTTCTTCTCTCTTATCTGCTTCTCTCCCATCCTCGCCGACTCCGACTTCGAAGGCTTCGAAGACGCCGACGATATCGACGAAGACTTGGAGTCCTCACTCCCTCTCACCACCACCCATCCATCCCAAACACTCCTCACCGCGACGGCCACCTCCCAACCCGACGCGGTGCCGGATCCCAGTGATCCTGTTCCGGATTCGGACCCAGCATCATCAACTACTGATTCTCCTAAACCTTCTTCCACCAGCTTTGAGTATTGGGACGAAGACGAGTTCGAAGGTCTACCACAAGATCAACCACAGCAACCGCCACTCACAGTTACTGAAAATGCTACTCCCTCTCCCGATTCCGATTCCGATTCCGATCTGAAACCCTCGACCGATCCGAAACAACCACCCGAGAGTTCTTCCAGATCTTACACGGTGGAGATCGCCTGCGGAGGGTTCTTGATCGTTTTCATCATCAATTACTTCACCGGAAAGCGCGTGAACGAGAACCTCGCCCTGGCATGGGCTGCCAAATTCGCAACCAAGGACACTATTTTCGACAAGAACTTCAGTCTTTTGGGCGTTGGCGAAGGCGATGACTCACCCTTGCTCTTGAAAGAAGGCCAGAACGTGTTCAAATTCTATGCCAGTGGGCGGAGGTGCTGCCAGGGGCTGTTGGCCACTATGGAGCTTAAGAGCCGCCACGATTTGATCTCTTCCATTTTCAATTTGGTGGTGCCTATTAGTAGAGACGAGATTAGTTTCGAGGTTTATATGAATGATGATGCCATGGACCACGTGGTTTTTGCTGTGGCCAAGAAGAAGGCCGCCAAAGCAATGCAGAAGGAGGTTCCCGACTTGCAAAAGTTCGCCACTATCGTTTCGCCTCCTACCGGCAGGAAGTGGGTTGCCGAAGACTTGGCGGTTATCTCTGAGTCCAAAGAGGTGGCTGGGGATTTAATCACTGAGGCTGTTCTTGATCAGGTTTTTGGTGAGAAAGCTTTTCAGAAATTTGGAAAGGGTTTCATGTATATGCATTTTTCAGATCAACATCCAGGGCCTACACACAAGAAGATGCTATTGTTCAAGTATGCACTACCTGATGCTGATAGCATGGCCGATATGACTCGTTTGGTGGCCTTGATACCTTACTATATTGATTTGATTGGTCGTTACAAGCTAAGCAGTCAGGTAGGCTTTTTTTACTTTTGGTCTGGACGGGTCAGCTATAAGTTATATTGTGGACAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

485

Amino Acids

54.37

Weight (kDa)

5.42

Isoelectric Point (pI)

54.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC47 PF07946 211 - 466 2.5e-65 PAT complex subunit CCDC47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 385, 940
AccI GTMKAC 1 cut(s) 499
AccII CGCG 3 cut(s) 359, 383, 702
AccIII TCCGGA 1 cut(s) 409
AclWI GGATC 4 cut(s) 386, 395, 399, 593
AcoI YGGCCR 4 cut(s) 363, 882, 1017, 1320
AcsI RAATTY 3 cut(s) 737, 842, 1214
AcuI CTGAAG 1 cut(s) 757
AcvI CACGTG 1 cut(s) 1003
AcyI GRCGYC 2 cut(s) 151, 282
AfiI CCNNNNNNNGG 6 cut(s) 657, 721, 726, 727, 858, 1097
AflII CTTAAG 1 cut(s) 896
AflIII ACRYGT 1 cut(s) 834
AgsI TTSAA 4 cut(s) 820, 841, 931, 1291
AhdI GACNNNNNGTC 1 cut(s) 307
AjnI CCWGG 3 cut(s) 720, 870, 1258
AluBI AGCT 6 cut(s) 465, 895, 1205, 1384, 1431, 1453
AluI AGCT 6 cut(s) 465, 895, 1205, 1384, 1431, 1453
Alw26I GTCTC 2 cut(s) 191, 948
AlwI GGATC 4 cut(s) 386, 395, 399, 593
AlwNI CAGNNNCTG 2 cut(s) 539, 877
Ama87I CYCGRG 1 cut(s) 614
Aor13HI TCCGGA 1 cut(s) 409
AoxI GGCC 8 cut(s) 363, 826, 882, 1017, 1029, 1262, 1320, 1341
ApeKI GCWGC 7 cut(s) 130, 133, 136, 139, 142, 731, 867
ApoI RAATTY 3 cut(s) 737, 842, 1214
ArsI GACNNNNNNTTYG 4 cut(s) 248, 280, 743, 775
AseI ATTAAT 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 836
AspLEI GCGC 1 cut(s) 702
AspS9I GGNCC 3 cut(s) 418, 997, 1262
AsuHPI GGTGA 5 cut(s) 316, 346, 682, 798, 1208
AsuII TTCGAA 3 cut(s) 267, 276, 492
AvaI CYCGRG 1 cut(s) 614
AvaII GGWCC 2 cut(s) 418, 997
BalI TGGCCA 2 cut(s) 884, 1019
BamHI GGATCC 1 cut(s) 391
BanI GGYRCC 2 cut(s) 385, 940
BbrPI CACGTG 1 cut(s) 1003
BbsI GAAGAC 4 cut(s) 285, 306, 489, 1125
BbvI GCAGC 7 cut(s) 142, 145, 148, 151, 154, 718, 854
BccI CCATC 4 cut(s) 61, 254, 342, 346
BceAI ACGGC 1 cut(s) 378
BciT130I CCWGG 3 cut(s) 722, 872, 1260
BclI TGATCA 1 cut(s) 1183
BcoDI GTCTC 2 cut(s) 191, 948
BfrI CTTAAG 1 cut(s) 896
BglII AGATCT 1 cut(s) 629
BlpI GCTNAGC 1 cut(s) 1385
Bme1390I CCNGG 3 cut(s) 722, 872, 1260
Bme18I GGWCC 2 cut(s) 418, 997
BmeRI GACNNNNNGTC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 614
BmgT120I GGNCC 3 cut(s) 418, 997, 1262
BmiI GGNNCC 5 cut(s) 387, 393, 420, 942, 1056
BmrFI CCNGG 3 cut(s) 722, 872, 1260
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 5 cut(s) 165, 434, 979, 1269, 1297
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 4 cut(s) 285, 306, 489, 1125
Bpu1102I GCTNAGC 1 cut(s) 1385
Bpu14I TTCGAA 3 cut(s) 267, 276, 492
BsaAI YACGTR 1 cut(s) 1003
BsaBI GATNNNNATC 2 cut(s) 579, 1254
BsaHI GRCGYC 2 cut(s) 151, 282
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 5 cut(s) 720, 747, 871, 993, 1259
BsaWI WCCGGW 2 cut(s) 409, 692
BsaXI ACNNNNNCTCC 4 cut(s) 427, 457, 541, 571
Bsc4I CCNNNNNNNGG 6 cut(s) 657, 721, 726, 727, 858, 1097
Bse118I RCCGGY 1 cut(s) 1097
Bse1I ACTGG 2 cut(s) 396, 852
Bse3DI GCAATG 1 cut(s) 1047
Bse8I GATNNNNATC 2 cut(s) 579, 1254
BseAI TCCGGA 1 cut(s) 409
BseBI CCWGG 3 cut(s) 722, 872, 1260
BseDI CCNNGG 5 cut(s) 720, 747, 871, 993, 1259
BseGI GGATG 4 cut(s) 246, 334, 338, 1255
BseJI GATNNNNATC 2 cut(s) 579, 1254
BseLI CCNNNNNNNGG 6 cut(s) 657, 721, 726, 727, 858, 1097
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 2 cut(s) 1128, 1161
BseNI ACTGG 2 cut(s) 396, 852
BseRI GAGGAG 2 cut(s) 194, 341
BseXI GCAGC 7 cut(s) 142, 145, 148, 151, 154, 718, 854
BseYI CCCAGC 2 cut(s) 421, 1154
Bsh1236I CGCG 3 cut(s) 359, 383, 702
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 8 cut(s) 365, 828, 884, 1019, 1031, 1264, 1322, 1343
BshNI GGYRCC 2 cut(s) 385, 940
BsiEI CGRYCG 1 cut(s) 597
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 4 cut(s) 389, 410, 693, 1098
BslFI GGGAC 2 cut(s) 179, 491
BslI CCNNNNNNNGG 6 cut(s) 657, 721, 726, 727, 858, 1097
BsmAI GTCTC 2 cut(s) 191, 948
BsmBI CGTCTC 1 cut(s) 948
BsmFI GGGAC 2 cut(s) 179, 491
BsnI GGCC 8 cut(s) 365, 828, 884, 1019, 1031, 1264, 1322, 1343
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 614
Bsp119I TTCGAA 3 cut(s) 267, 276, 492
Bsp13I TCCGGA 1 cut(s) 409
Bsp1720I GCTNAGC 1 cut(s) 1385
Bsp19I CCATGG 1 cut(s) 993
BspANI GGCC 8 cut(s) 365, 828, 884, 1019, 1031, 1264, 1322, 1343
BspCNI CTCAG 2 cut(s) 1129, 1162
BspEI TCCGGA 1 cut(s) 409
BspFNI CGCG 3 cut(s) 359, 383, 702
BspLI GGNNCC 5 cut(s) 387, 393, 420, 942, 1056
BspPI GGATC 4 cut(s) 386, 395, 399, 593
BspQI GCTCTTC 1 cut(s) 96
BspT104I TTCGAA 3 cut(s) 267, 276, 492
BspT107I GGYRCC 2 cut(s) 385, 940
BspTI CTTAAG 1 cut(s) 896
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 1 cut(s) 1097
BsrI ACTGG 2 cut(s) 396, 852
BssAI RCCGGY 1 cut(s) 1097
BssECI CCNNGG 5 cut(s) 720, 747, 871, 993, 1259
BssNI GRCGYC 2 cut(s) 151, 282
BssT1I CCWWGG 2 cut(s) 747, 993
Bst2UI CCWGG 3 cut(s) 722, 872, 1260
Bst4CI ACNGT 2 cut(s) 535, 640
Bst6I CTCTTC 5 cut(s) 91, 96, 218, 224, 925
BstACI GRCGYC 2 cut(s) 151, 282
BstAFI CTTAAG 1 cut(s) 896
BstAPI GCANNNNNTGC 1 cut(s) 731
BstBAI YACGTR 1 cut(s) 1003
BstBI TTCGAA 3 cut(s) 267, 276, 492
BstC8I GCNNGC 1 cut(s) 652
BstDEI CTNAG 3 cut(s) 1137, 1170, 1385
BstDSI CCRYGG 1 cut(s) 993
BstF5I GGATG 4 cut(s) 246, 334, 338, 1255
BstFNI CGCG 3 cut(s) 359, 383, 702
BstHHI GCGC 1 cut(s) 702
BstMAI GTCTC 2 cut(s) 191, 948
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 8 cut(s) 136, 139, 142, 145, 148, 731, 740, 1037
BstNI CCWGG 3 cut(s) 722, 872, 1260
BstSCI CCNGG 3 cut(s) 720, 870, 1258
BstUI CGCG 3 cut(s) 359, 383, 702
BstV1I GCAGC 7 cut(s) 142, 145, 148, 151, 154, 718, 854
BstV2I GAAGAC 4 cut(s) 285, 306, 489, 1125
BstX2I RGATCY 2 cut(s) 391, 629
BstXI CCANNNNNNTGG 1 cut(s) 1150
BstYI RGATCY 2 cut(s) 391, 629
BsuRI GGCC 8 cut(s) 365, 828, 884, 1019, 1031, 1264, 1322, 1343
BtgI CCRYGG 1 cut(s) 993
BtgZI GCGATG 1 cut(s) 813
BtsCI GGATG 4 cut(s) 246, 334, 338, 1255
BtsIMutI CAGTG 3 cut(s) 403, 859, 1167
Cac8I GCNNGC 1 cut(s) 652
CaiI CAGNNNCTG 2 cut(s) 539, 877
CfoI GCGC 1 cut(s) 702
Cfr10I RCCGGY 1 cut(s) 1097
Cfr13I GGNCC 3 cut(s) 418, 997, 1262
CseI GACGC 3 cut(s) 140, 290, 389
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 5 cut(s) 181, 726, 994, 1231, 1318
DdeI CTNAG 3 cut(s) 1137, 1170, 1385
DriI GACNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 4 cut(s) 363, 882, 1017, 1320
Eam1104I CTCTTC 5 cut(s) 91, 96, 218, 224, 925
Eam1105I GACNNNNNGTC 1 cut(s) 307
EarI CTCTTC 5 cut(s) 91, 96, 218, 224, 925
EciI GGCGGA 1 cut(s) 874
Eco130I CCWWGG 2 cut(s) 747, 993
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 418, 997
Eco57I CTGAAG 1 cut(s) 757
Eco72I CACGTG 1 cut(s) 1003
Eco88I CYCGRG 1 cut(s) 614
EcoO109I RGGNCCY 1 cut(s) 1262
EcoRII CCWGG 3 cut(s) 720, 870, 1258
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 2 cut(s) 747, 993
EcoT22I ATGCAT 1 cut(s) 1242
ErhI CCWWGG 2 cut(s) 747, 993
Esp3I CGTCTC 1 cut(s) 948
FaeI CATG 5 cut(s) 184, 729, 997, 1234, 1321
FaqI GGGAC 2 cut(s) 179, 491
FatI CATG 5 cut(s) 180, 725, 993, 1230, 1317
FbaI TGATCA 1 cut(s) 1183
FblI GTMKAC 1 cut(s) 499
FokI GGATG 4 cut(s) 233, 321, 325, 1242
GlaI GCGC 1 cut(s) 701
GsaI CCCAGC 2 cut(s) 425, 1158
HaeIII GGCC 8 cut(s) 365, 828, 884, 1019, 1031, 1264, 1322, 1343
HapII CCGG 4 cut(s) 389, 410, 693, 1098
HgaI GACGC 3 cut(s) 140, 290, 389
HhaI GCGC 1 cut(s) 702
Hin1I GRCGYC 2 cut(s) 151, 282
Hin1II CATG 5 cut(s) 184, 729, 997, 1234, 1321
Hin6I GCGC 1 cut(s) 700
HinP1I GCGC 1 cut(s) 700
HindIII AAGCTT 1 cut(s) 1203
HpaII CCGG 4 cut(s) 389, 410, 693, 1098
HphI GGTGA 5 cut(s) 316, 346, 682, 798, 1208
Hpy166II GTNNAC 3 cut(s) 500, 706, 1448
Hpy188III TCNNGA 8 cut(s) 410, 560, 627, 664, 817, 1058, 1181, 1419
Hpy8I GTNNAC 3 cut(s) 500, 706, 1448
Hpy99I CGWCG 5 cut(s) 156, 290, 299, 364, 383
HpyAV CCTTC 7 cut(s) 263, 462, 488, 788, 818, 1021, 1042
HpyCH4III ACNGT 2 cut(s) 535, 640
HpyCH4IV ACGT 2 cut(s) 834, 1002
HpyCH4V TGCA 5 cut(s) 130, 1045, 1066, 1240, 1298
HpyF10VI GCNNNNNNNGC 8 cut(s) 136, 139, 142, 145, 148, 731, 740, 1037
HpyF3I CTNAG 3 cut(s) 1137, 1170, 1385
HpySE526I ACGT 2 cut(s) 834, 1002
Hsp92I GRCGYC 2 cut(s) 151, 282
Hsp92II CATG 5 cut(s) 184, 729, 997, 1234, 1321
HspAI GCGC 1 cut(s) 700
Kpn2I TCCGGA 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 1183
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 1 cut(s) 892
Lsp1109I GCAGC 7 cut(s) 142, 145, 148, 151, 154, 718, 854
LweI GCATC 5 cut(s) 165, 434, 979, 1269, 1297
MaeII ACGT 2 cut(s) 834, 1002
MaeIII GTNAC 3 cut(s) 82, 535, 1376
MflI RGATCY 2 cut(s) 391, 629
MlsI TGGCCA 2 cut(s) 884, 1019
MluCI AATT 6 cut(s) 67, 682, 737, 842, 931, 1214
MluNI TGGCCA 2 cut(s) 884, 1019
MlyI GAGTC 5 cut(s) 251, 317, 797, 1148, 1324
MmeI TCCRAC 1 cut(s) 285
Mox20I TGGCCA 2 cut(s) 884, 1019
Mph1103I ATGCAT 1 cut(s) 1242
MroI TCCGGA 1 cut(s) 409
MroXI GAANNNNTTC 1 cut(s) 836
MscI TGGCCA 2 cut(s) 884, 1019
MseI TTAA 3 cut(s) 66, 897, 1163
MslI CAYNNNNRTG 2 cut(s) 179, 1235
Msp20I TGGCCA 2 cut(s) 884, 1019
MspA1I CMGCKG 2 cut(s) 40, 145
MspCI CTTAAG 1 cut(s) 896
MspI CCGG 4 cut(s) 389, 410, 693, 1098
MspR9I CCNGG 3 cut(s) 722, 872, 1260
MvaI CCWGG 3 cut(s) 722, 872, 1260
MvnI CGCG 3 cut(s) 359, 383, 702
MwoI GCNNNNNNNGC 8 cut(s) 136, 139, 142, 145, 148, 731, 740, 1037
NcoI CCATGG 1 cut(s) 993
NlaIII CATG 5 cut(s) 184, 729, 997, 1234, 1321
NlaIV GGNNCC 5 cut(s) 387, 393, 420, 942, 1056
NsiI ATGCAT 1 cut(s) 1242
NspV TTCGAA 3 cut(s) 267, 276, 492
PciSI GCTCTTC 1 cut(s) 96
PcsI WCGNNNNNNNCGW 6 cut(s) 258, 273, 282, 294, 599, 1080
PdmI GAANNNNTTC 1 cut(s) 836
PfeI GAWTC 7 cut(s) 43, 73, 413, 440, 563, 569, 575
PleI GAGTC 5 cut(s) 251, 316, 797, 1147, 1324
PmaCI CACGTG 1 cut(s) 1003
PmlI CACGTG 1 cut(s) 1003
PpsI GAGTC 5 cut(s) 251, 316, 797, 1147, 1324
Ppu21I YACGTR 1 cut(s) 1003
PshBI ATTAAT 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 720, 870, 1258
PspCI CACGTG 1 cut(s) 1003
PspFI CCCAGC 2 cut(s) 421, 1154
PspGI CCWGG 3 cut(s) 720, 870, 1258
PspN4I GGNNCC 5 cut(s) 387, 393, 420, 942, 1056
PspPI GGNCC 3 cut(s) 418, 997, 1262
PstNI CAGNNNCTG 2 cut(s) 539, 877
PsuI RGATCY 2 cut(s) 391, 629
RseI CAYNNNNRTG 2 cut(s) 179, 1235
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 3 cut(s) 66, 897, 1163
Sau96I GGNCC 3 cut(s) 418, 997, 1262
SchI GAGTC 5 cut(s) 251, 317, 797, 1148, 1324
ScrFI CCNGG 3 cut(s) 722, 872, 1260
SfaNI GCATC 5 cut(s) 165, 434, 979, 1269, 1297
SfuI TTCGAA 3 cut(s) 267, 276, 492
SinI GGWCC 2 cut(s) 418, 997
SmiMI CAYNNNNRTG 2 cut(s) 179, 1235
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
Sse9I AATT 6 cut(s) 67, 682, 737, 842, 931, 1214
SspI AATATT 1 cut(s) 64
StyD4I CCNGG 3 cut(s) 720, 870, 1258
StyI CCWWGG 2 cut(s) 747, 993
TaaI ACNGT 2 cut(s) 535, 640
TaiI ACGT 2 cut(s) 837, 1005
TaqI TCGA 7 cut(s) 267, 276, 294, 492, 593, 762, 969
TaqII GACCGA 1 cut(s) 611
TasI AATT 6 cut(s) 67, 682, 737, 842, 931, 1214
TauI GCSGC 4 cut(s) 148, 151, 906, 1034
TfiI GAWTC 7 cut(s) 43, 73, 413, 440, 563, 569, 575
Tru1I TTAA 3 cut(s) 66, 897, 1163
Tru9I TTAA 3 cut(s) 66, 897, 1163
TscAI CASTG 3 cut(s) 403, 859, 1174
TseI GCWGC 7 cut(s) 130, 133, 136, 139, 142, 731, 867
TspDTI ATGAA 3 cut(s) 664, 995, 1219
TspRI CASTG 3 cut(s) 403, 859, 1174
Vha464I CTTAAG 1 cut(s) 896
VpaK11BI GGWCC 2 cut(s) 418, 997
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 3 cut(s) 737, 842, 1214
XcmI CCANNNNNNNNNTGG 2 cut(s) 931, 1000
XmiI GTMKAC 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 836
Zsp2I ATGCAT 1 cut(s) 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.