FvH4_5g28340

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007803
Physical Location & Seq
Reverse (-)
19488644 .. 19493953
5310 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28340.t5

Sequence Viewer

Length: 1086 bp
ATGGGACATCTCCTGACTCTCTTCCTCTTCTTCTCTCTTATCTGCCTCTCTCCCATCCTGGCCGACTCCGACTTCGAAGGCTTCGAAGACGCTGACGATGTCGACGAAGACTTGGAGTCGTCACTCCCTCTCACCGCCCAAACAGTCCTGACCTCCCAACCCGACCCGGATCCAACTCCTAAAGCTGGCAGCTTCGAGTATTGGGACGAAGACGAGTTCGAAGGTCTGCCCCAGGATCAGCCGCCAGTCGTAGCCACAGAAGAAAACGCTGATCCGAAACCCTCGAAGACTGAAGCCGCGCCCGAGGGTTCTTCCTCCTCCAGATCTTACACCGTGGAGATCGCCTGCGGAGGGTTCCTCATCGTCTTCGTCGTCAATTACTTCACCGGAAAGCGCGTGAACGAGAACATTGCCCTGGCCTGGGCTGCCAAATTCGCTACCAAGGACACTATTTTCGACAAGAACTTCAGTCTCTTAGGCGTTGGCGACGGCGACGACTCACCTCTGCTCTTGAAGGAAGGCCAGAACGTGTTCAAATTCTACGCCAGTGGCCGCAGGTGCTGCCAGGGCCTCTTGGCCACCATGGAGCTCAAGAGCCGCCACGATTTGATCTCCTCCATTTTCAATTTGGTGATCCCTACTAGTAGGGACGAGATTAGTTTCGAGGTTTATATGAATGATGATGCCATGGACCACGTCGTTTTTGCTGTGGCCAACAACAAGTCCGCCAAGGCAATGCACAAGGAGCTTCCCGACTTGCACAAGTTTGCCACTATTCTTTCCCCTCCTACTGCCAGGAAGTGGGTTGGCCATGACTTGGCCGTTATCTCCGAGTCCAAAGAGGTTGCTGCCGATCTAATCACTGAGGCTGTTCTTGATCAGGTTTTTGGAGAGAAAGCTTTTCAGAAGTTTGGAAAGGGTTTCATGTATATGCATTTTTCAGATCAACATCCAGGGCCTACAAACAAGAAGATGCTATTGTTCAAGTTTGCACTACCTGATGCTAATAGCATGGCTGATATGACTCGTTTGGTAGGTCAGCTACTGTTTTTTAACTTTTTGTCTGGACGGGTCAGCTATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

362

Amino Acids

39.98

Weight (kDa)

4.72

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC47 PF07946 109 - 293 1.2e-49 PAT complex subunit CCDC47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 546
Acc36I ACCTGC 1 cut(s) 546
AccB7I CCANNNNNTGG 2 cut(s) 801, 817
AccI GTMKAC 1 cut(s) 102
AccII CGCG 2 cut(s) 299, 396
AciI CCGC 7 cut(s) 135, 242, 297, 348, 553, 598, 726
AclWI GGATC 5 cut(s) 164, 177, 243, 266, 628
AcoI YGGCCR 6 cut(s) 60, 550, 576, 711, 808, 819
AcsI RAATTY 2 cut(s) 431, 536
AcuI CTGAAG 2 cut(s) 312, 451
AfiI CCNNNNNNNGG 6 cut(s) 185, 351, 420, 421, 801, 817
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 4 cut(s) 514, 535, 625, 985
AhdI GACNNNNNGTC 1 cut(s) 115
AhlI ACTAGT 1 cut(s) 641
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 7 cut(s) 57, 231, 414, 419, 564, 794, 952
AluBI AGCT 7 cut(s) 185, 192, 589, 748, 899, 1042, 1077
AluI AGCT 7 cut(s) 185, 192, 589, 748, 899, 1042, 1077
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 5 cut(s) 164, 177, 243, 266, 628
AlwNI CAGNNNCTG 2 cut(s) 561, 1045
Ama87I CYCGRG 1 cut(s) 302
ApeKI GCWGC 4 cut(s) 189, 425, 561, 848
ApoI RAATTY 2 cut(s) 431, 536
ArsI GACNNNNNNTTYG 4 cut(s) 56, 88, 437, 469
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 2 cut(s) 301, 396
AspS9I GGNCC 3 cut(s) 568, 691, 956
AsuC2I CCSGG 1 cut(s) 167
AsuHPI GGTGA 4 cut(s) 124, 376, 492, 643
AsuII TTCGAA 3 cut(s) 75, 84, 219
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 691
BalI TGGCCA 3 cut(s) 578, 713, 810
BamHI GGATCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 591
BbsI GAAGAC 5 cut(s) 93, 114, 216, 293, 358
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 4 cut(s) 201, 412, 548, 835
BccI CCATC 1 cut(s) 62
BceAI ACGGC 2 cut(s) 505, 806
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 7 cut(s) 59, 233, 416, 421, 566, 796, 954
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 476
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 1 cut(s) 642
BfuAI ACCTGC 1 cut(s) 546
BglII AGATCT 1 cut(s) 323
BisI GCNGC 8 cut(s) 190, 242, 297, 426, 553, 562, 598, 849
BlsI GCNGC 8 cut(s) 191, 243, 298, 427, 554, 563, 599, 850
Bme1390I CCNGG 8 cut(s) 59, 167, 233, 416, 421, 566, 796, 954
Bme18I GGWCC 1 cut(s) 691
BmeRI GACNNNNNGTC 1 cut(s) 115
BmeT110I CYCGRG 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 697
BmgT120I GGNCC 3 cut(s) 568, 691, 956
BmiI GGNNCC 2 cut(s) 171, 356
BmrFI CCNGG 8 cut(s) 59, 167, 233, 416, 421, 566, 796, 954
BmsI GCATC 3 cut(s) 673, 963, 991
BpiI GAAGAC 5 cut(s) 93, 114, 216, 293, 358
BplI GAGNNNNNCTC 2 cut(s) 342, 374
BpmI CTGGAG 1 cut(s) 304
Bpu14I TTCGAA 3 cut(s) 75, 84, 219
BpuEI CTTGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 167
BsaBI GATNNNNATC 1 cut(s) 948
BsaWI WCCGGW 1 cut(s) 386
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 351, 420, 421, 801, 817
Bse1I ACTGG 2 cut(s) 245, 546
Bse3DI GCAATG 2 cut(s) 408, 741
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 7 cut(s) 59, 233, 416, 421, 566, 796, 954
BseGI GGATG 2 cut(s) 54, 949
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 6 cut(s) 185, 351, 420, 421, 801, 817
BseMI GCAATG 2 cut(s) 408, 741
BseMII CTCAG 1 cut(s) 855
BseNI ACTGG 2 cut(s) 245, 546
BseRI GAGGAG 2 cut(s) 307, 604
BseXI GCAGC 4 cut(s) 201, 412, 548, 835
Bsh1236I CGCG 2 cut(s) 299, 396
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 2 cut(s) 167, 387
BslFI GGGAC 3 cut(s) 18, 218, 662
BslI CCNNNNNNNGG 6 cut(s) 185, 351, 420, 421, 801, 817
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 3 cut(s) 18, 218, 662
BsoBI CYCGRG 1 cut(s) 302
Bsp119I TTCGAA 3 cut(s) 75, 84, 219
Bsp1286I GDGCHC 1 cut(s) 591
Bsp19I CCATGG 2 cut(s) 582, 687
BspACI CCGC 7 cut(s) 135, 242, 297, 348, 553, 598, 726
BspCNI CTCAG 1 cut(s) 856
BspFNI CGCG 2 cut(s) 299, 396
BspLI GGNNCC 2 cut(s) 171, 356
BspMI ACCTGC 1 cut(s) 546
BspPI GGATC 5 cut(s) 164, 177, 243, 266, 628
BspT104I TTCGAA 3 cut(s) 75, 84, 219
BsrDI GCAATG 2 cut(s) 408, 741
BsrI ACTGG 2 cut(s) 245, 546
BssT1I CCWWGG 4 cut(s) 441, 582, 687, 729
Bst2UI CCWGG 7 cut(s) 59, 233, 416, 421, 566, 796, 954
Bst4CI ACNGT 3 cut(s) 145, 334, 1047
Bst6I CTCTTC 2 cut(s) 26, 32
BstAPI GCANNNNNTGC 1 cut(s) 561
BstBI TTCGAA 3 cut(s) 75, 84, 219
BstC8I GCNNGC 2 cut(s) 187, 346
BstDEI CTNAG 2 cut(s) 475, 864
BstDSI CCRYGG 3 cut(s) 333, 582, 687
BstF5I GGATG 2 cut(s) 54, 949
BstFNI CGCG 2 cut(s) 299, 396
BstHHI GCGC 2 cut(s) 301, 396
BstMAI GTCTC 1 cut(s) 476
BstMWI GCNNNNNNNGC 6 cut(s) 425, 434, 558, 561, 567, 745
BstNI CCWGG 7 cut(s) 59, 233, 416, 421, 566, 796, 954
BstSCI CCNGG 8 cut(s) 57, 165, 231, 414, 419, 564, 794, 952
BstUI CGCG 2 cut(s) 299, 396
BstV1I GCAGC 4 cut(s) 201, 412, 548, 835
BstV2I GAAGAC 5 cut(s) 93, 114, 216, 293, 358
BstX2I RGATCY 2 cut(s) 169, 323
BstYI RGATCY 2 cut(s) 169, 323
BtgI CCRYGG 3 cut(s) 333, 582, 687
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 2 cut(s) 54, 949
BtsIMutI CAGTG 2 cut(s) 553, 861
BveI ACCTGC 1 cut(s) 546
Cac8I GCNNGC 2 cut(s) 187, 346
CaiI CAGNNNCTG 2 cut(s) 561, 1045
CfoI GCGC 2 cut(s) 301, 396
Cfr13I GGNCC 3 cut(s) 568, 691, 956
CseI GACGC 1 cut(s) 98
CviAII CATG 5 cut(s) 583, 688, 812, 925, 1012
DdeI CTNAG 2 cut(s) 475, 864
DriI GACNNNNNGTC 1 cut(s) 115
EaeI YGGCCR 6 cut(s) 60, 550, 576, 711, 808, 819
Eam1104I CTCTTC 2 cut(s) 26, 32
Eam1105I GACNNNNNGTC 1 cut(s) 115
EarI CTCTTC 2 cut(s) 26, 32
EciI GGCGGA 1 cut(s) 715
Ecl136II GAGCTC 1 cut(s) 589
Eco130I CCWWGG 4 cut(s) 441, 582, 687, 729
Eco24I GRGCYC 1 cut(s) 591
Eco47I GGWCC 1 cut(s) 691
Eco53kI GAGCTC 1 cut(s) 589
Eco57I CTGAAG 2 cut(s) 312, 451
Eco88I CYCGRG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 589
EcoO109I RGGNCCY 2 cut(s) 568, 956
EcoRII CCWGG 7 cut(s) 57, 231, 414, 419, 564, 794, 952
EcoT14I CCWWGG 4 cut(s) 441, 582, 687, 729
EcoT22I ATGCAT 1 cut(s) 936
EcoT38I GRGCYC 1 cut(s) 591
ErhI CCWWGG 4 cut(s) 441, 582, 687, 729
FaeI CATG 5 cut(s) 586, 691, 815, 928, 1015
FaqI GGGAC 3 cut(s) 18, 218, 662
FatI CATG 5 cut(s) 582, 687, 811, 924, 1011
FbaI TGATCA 1 cut(s) 877
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 8 cut(s) 190, 242, 297, 426, 553, 562, 598, 849
FokI GGATG 2 cut(s) 41, 936
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 8 cut(s) 190, 242, 297, 426, 553, 562, 598, 849
FspBI CTAG 1 cut(s) 642
GlaI GCGC 2 cut(s) 300, 395
GluI GCNGC 8 cut(s) 190, 242, 297, 426, 553, 562, 598, 849
GsuI CTGGAG 1 cut(s) 304
HapII CCGG 2 cut(s) 167, 387
HgaI GACGC 1 cut(s) 98
HhaI GCGC 2 cut(s) 301, 396
Hin1II CATG 5 cut(s) 586, 691, 815, 928, 1015
Hin6I GCGC 2 cut(s) 299, 394
HinP1I GCGC 2 cut(s) 299, 394
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 897
HinfI GANTC 6 cut(s) 16, 65, 116, 497, 833, 1024
HpaII CCGG 2 cut(s) 167, 387
HphI GGTGA 4 cut(s) 124, 376, 492, 643
Hpy166II GTNNAC 2 cut(s) 103, 400
Hpy188I TCNGA 5 cut(s) 70, 276, 832, 906, 943
Hpy188III TCNNGA 8 cut(s) 13, 148, 321, 511, 592, 752, 875, 1065
Hpy8I GTNNAC 2 cut(s) 103, 400
Hpy99I CGWCG 5 cut(s) 107, 374, 491, 497, 701
HpyAV CCTTC 4 cut(s) 71, 215, 508, 512
HpyCH4III ACNGT 3 cut(s) 145, 334, 1047
HpyCH4IV ACGT 2 cut(s) 528, 696
HpyCH4V TGCA 4 cut(s) 739, 760, 934, 992
HpyF10VI GCNNNNNNNGC 6 cut(s) 425, 434, 558, 561, 567, 745
HpyF3I CTNAG 2 cut(s) 475, 864
HpySE526I ACGT 2 cut(s) 528, 696
Hsp92II CATG 5 cut(s) 586, 691, 815, 928, 1015
HspAI GCGC 2 cut(s) 299, 394
Ksp22I TGATCA 1 cut(s) 877
LmnI GCTCC 2 cut(s) 586, 745
Lsp1109I GCAGC 4 cut(s) 201, 412, 548, 835
LweI GCATC 3 cut(s) 673, 963, 991
MaeI CTAG 1 cut(s) 642
MaeII ACGT 2 cut(s) 528, 696
MaeIII GTNAC 1 cut(s) 120
MflI RGATCY 2 cut(s) 169, 323
MhlI GDGCHC 1 cut(s) 591
MlsI TGGCCA 3 cut(s) 578, 713, 810
MluCI AATT 4 cut(s) 376, 431, 536, 625
MluNI TGGCCA 3 cut(s) 578, 713, 810
MlyI GAGTC 6 cut(s) 10, 59, 125, 491, 842, 1018
MmeI TCCRAC 2 cut(s) 93, 197
Mox20I TGGCCA 3 cut(s) 578, 713, 810
Mph1103I ATGCAT 1 cut(s) 936
MroXI GAANNNNTTC 1 cut(s) 530
MscI TGGCCA 3 cut(s) 578, 713, 810
MseI TTAA 1 cut(s) 1053
MslI CAYNNNNRTG 1 cut(s) 929
Msp20I TGGCCA 3 cut(s) 578, 713, 810
MspI CCGG 2 cut(s) 167, 387
MspR9I CCNGG 8 cut(s) 59, 167, 233, 416, 421, 566, 796, 954
MvaI CCWGG 7 cut(s) 59, 233, 416, 421, 566, 796, 954
MvnI CGCG 2 cut(s) 299, 396
MwoI GCNNNNNNNGC 6 cut(s) 425, 434, 558, 561, 567, 745
NciI CCSGG 1 cut(s) 167
NcoI CCATGG 2 cut(s) 582, 687
NlaIII CATG 5 cut(s) 586, 691, 815, 928, 1015
NlaIV GGNNCC 2 cut(s) 171, 356
NmuCI GTSAC 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 936
NspV TTCGAA 3 cut(s) 75, 84, 219
PaqCI CACCTGC 1 cut(s) 546
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 102, 369
PdmI GAANNNNTTC 1 cut(s) 530
PflFI GACNNNGTC 2 cut(s) 98, 695
PflMI CCANNNNNTGG 2 cut(s) 801, 817
PkrI GCNGC 8 cut(s) 191, 243, 298, 427, 554, 563, 599, 850
PleI GAGTC 6 cut(s) 10, 59, 124, 491, 841, 1018
PpsI GAGTC 6 cut(s) 10, 59, 124, 491, 841, 1018
Psp124BI GAGCTC 1 cut(s) 591
Psp6I CCWGG 7 cut(s) 57, 231, 414, 419, 564, 794, 952
PspGI CCWGG 7 cut(s) 57, 231, 414, 419, 564, 794, 952
PspN4I GGNNCC 2 cut(s) 171, 356
PspPI GGNCC 3 cut(s) 568, 691, 956
PstNI CAGNNNCTG 2 cut(s) 561, 1045
PsuI RGATCY 2 cut(s) 169, 323
PsyI GACNNNGTC 2 cut(s) 98, 695
RseI CAYNNNNRTG 1 cut(s) 929
SacI GAGCTC 1 cut(s) 591
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 1 cut(s) 1053
SatI GCNGC 8 cut(s) 190, 242, 297, 426, 553, 562, 598, 849
Sau96I GGNCC 3 cut(s) 568, 691, 956
SchI GAGTC 6 cut(s) 10, 59, 125, 491, 842, 1018
ScrFI CCNGG 8 cut(s) 59, 167, 233, 416, 421, 566, 796, 954
SduI GDGCHC 1 cut(s) 591
SfaNI GCATC 3 cut(s) 673, 963, 991
SfuI TTCGAA 3 cut(s) 75, 84, 219
SinI GGWCC 1 cut(s) 691
SmiMI CAYNNNNRTG 1 cut(s) 929
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
SpeI ACTAGT 1 cut(s) 641
Sse9I AATT 4 cut(s) 376, 431, 536, 625
SsiI CCGC 7 cut(s) 135, 242, 297, 348, 553, 598, 726
SspMI CTAG 1 cut(s) 642
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 8 cut(s) 57, 165, 231, 414, 419, 564, 794, 952
StyI CCWWGG 4 cut(s) 441, 582, 687, 729
TaaI ACNGT 3 cut(s) 145, 334, 1047
TaiI ACGT 2 cut(s) 531, 699
TaqI TCGA 8 cut(s) 75, 84, 102, 195, 219, 284, 456, 663
TasI AATT 4 cut(s) 376, 431, 536, 625
TauI GCSGC 4 cut(s) 244, 299, 555, 600
Tru1I TTAA 1 cut(s) 1053
Tru9I TTAA 1 cut(s) 1053
TscAI CASTG 2 cut(s) 553, 868
TseFI GTSAC 1 cut(s) 120
TseI GCWGC 4 cut(s) 189, 425, 561, 848
Tsp45I GTSAC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 689, 913
TspRI CASTG 2 cut(s) 553, 868
Tth111I GACNNNGTC 2 cut(s) 98, 695
Van91I CCANNNNNTGG 2 cut(s) 801, 817
VpaK11BI GGWCC 1 cut(s) 691
XapI RAATTY 2 cut(s) 431, 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 1 cut(s) 642
Zsp2I ATGCAT 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.