RchiOBHm_Chr7g0220701

At5g49945-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007803
Physical Location & Seq
Forward (+)
39898277 .. 39903068
4792 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19753

Sequence Viewer

Length: 1440 bp
ATGGGTCTCGCTGTCCCACATCTCCTCACTCTCTTCCTATTCTTCTCTCTTATCTGCTTCTCTCCCATCCTCGCCGACTCCGACTTCGAAGGCTTCGAAGACGCCGACGATATCGACGAAGACTTGGACTCCTCACTCCCTCTCACCACCACCCATCCATCCCAAACACTCCTCACCGCGACGGCCACCTCCCAACCCGACCCGGTGCCGGATCCCAGTGATCCTGTTCAGGATTCGGACCCAGCATCATCAACTACTGATTCTCCTAAACCTTCTTCCACCAGCTTTGAGTATTGGGACGAAGACGAGTTCGAAGGTCTACCACAAGATCAACCACAGCAACCGCCACTCACAGTCACTGAAAATGCTACTCCGTCTCCCGATTCCGATTCCGATCTGAAACCCTCGACCGATCCGAAACAACCACCCGAGAGTTCTTCCAGATCTTACACGGTGGAGATCGCCTGCGGAGGGTTCTTGATCGTTTTCATCATCAATTACTTCACCGGAAAGCGCATGAACGAGAACCTCGCCCTGGCATGGGCTGCCAAATTCGCAACCAAGGACACTATTTTCGACAAGAACTTCAGTCTTTTGGGCGTTGGCGAAGGCGATGACTCACCCTTGCTCTTGAAAGAAGGCCAGAACGTGTTCAAATTCTATGCCAGTGGGCGGAGGTGCTGCCAGGGGCTGTTGGCCACTATGGAGCTTAAGAGCCGCCACGATTTGATCTCTTCCATTTTCAATTTGGTGGTGCCTATTAGTAGAGACGAGATTAGTTTCGAGGTTTACATGAATGATGATGCCATGGACCACGTGGTTTTTGCTGTGGCCAAGAAGAAGGCCGCCAAAGCAATGCAGAAGGAGGTTCCCGACTTGCAAAAGTTCGCCACTATCCTTTCGCCTCCTGCCGGCAGGAAGTGGGTTGCCGAAGACTTGGCAGTTATCTCTGAGTCCAAAGAGGTGGCTGGGGATTTAATCACTGAGGCTGTTCTTGATCAGGTTTTTGGTGAGAAAGCTTTTCAGAAATTTGGAAAGGGTTTCATGTATATGCATTTTTCAGATCAACATCCAGGGCCTACACACAAGAAGATGCTATTGTTCAAGTATGCACTACCTGATGCTGATAGCATGGCCGATATGACTCGTTTGGTGGCCTTGATACCTTACTATATTGATTTGATTGGTCGTTACAAGCTAAGCAGTCAGGCCCGATCCAAAACAGAAGCAACCAGATCAAAGGCTGCTCAAGAGGCATACAAAGAACTCCAAAACGCTAGGCAAGAAGCTTTGCAAAGAAAGAAGGCAGAGAGGAAAAAATTGATGGAGGAGGCTGAGGCTAAGCTTAGTGCAGAGAGTATTCGCAAGAAGGAAGCAAAGGAGCGTGCTCGTCAAATGAAGAAGGCAATGCCTAAAATGAAAATGACCCGTGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1682

Protein Analysis

479

Amino Acids

53.29

Weight (kDa)

5.18

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC47 PF07946 149 - 468 3.4e-86 PAT complex subunit CCDC47
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 205, 754
AccI GTMKAC 1 cut(s) 319
AccII CGCG 1 cut(s) 179
AciI CCGC 6 cut(s) 177, 344, 468, 673, 718, 846
AclWI GGATC 5 cut(s) 206, 215, 219, 407, 1209
AcoI YGGCCR 4 cut(s) 183, 696, 831, 1134
AcsI RAATTY 3 cut(s) 551, 656, 1028
AcuI CTGAAG 1 cut(s) 571
AcvI CACGTG 1 cut(s) 817
AcyI GRCGYC 1 cut(s) 102
AfiI CCNNNNNNNGG 7 cut(s) 208, 471, 535, 540, 541, 672, 911
AflII CTTAAG 1 cut(s) 710
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 4 cut(s) 634, 655, 745, 1105
AjnI CCWGG 3 cut(s) 534, 684, 1072
AluBI AGCT 7 cut(s) 285, 709, 1019, 1198, 1289, 1345, 1437
AluI AGCT 7 cut(s) 285, 709, 1019, 1198, 1289, 1345, 1437
Alw21I GWGCWC 1 cut(s) 1390
Alw26I GTCTC 3 cut(s) 11, 381, 762
AlwI GGATC 5 cut(s) 206, 215, 219, 407, 1209
AlwNI CAGNNNCTG 2 cut(s) 359, 691
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 9 cut(s) 183, 640, 696, 831, 843, 1076, 1134, 1155, 1209
ApeKI GCWGC 3 cut(s) 545, 681, 1244
ApoI RAATTY 3 cut(s) 551, 656, 1028
ArsI GACNNNNNNTTYG 4 cut(s) 68, 100, 557, 589
Asp700I GAANNNNTTC 1 cut(s) 650
AspLEI GCGC 1 cut(s) 516
AspS9I GGNCC 4 cut(s) 238, 811, 1076, 1210
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 5 cut(s) 136, 166, 496, 612, 1022
AsuII TTCGAA 3 cut(s) 87, 96, 312
AsuNHI GCTAGC 1 cut(s) 1433
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 2 cut(s) 238, 811
BalI TGGCCA 2 cut(s) 698, 833
BamHI GGATCC 1 cut(s) 211
BanI GGYRCC 2 cut(s) 205, 754
BbrPI CACGTG 1 cut(s) 817
BbsI GAAGAC 4 cut(s) 105, 126, 309, 939
Bbv12I GWGCWC 1 cut(s) 1390
BbvCI CCTCAGC 1 cut(s) 1335
BbvI GCAGC 3 cut(s) 532, 668, 1231
BccI CCATC 4 cut(s) 74, 162, 166, 1318
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 3 cut(s) 536, 686, 1074
BclI TGATCA 1 cut(s) 997
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 3 cut(s) 11, 381, 762
BfaI CTAG 2 cut(s) 1278, 1434
BfrI CTTAAG 1 cut(s) 710
BglII AGATCT 1 cut(s) 443
BisI GCNGC 5 cut(s) 546, 682, 718, 846, 1245
BlpI GCTNAGC 2 cut(s) 1199, 1341
BlsI GCNGC 5 cut(s) 547, 683, 719, 847, 1246
Bme1390I CCNGG 4 cut(s) 203, 536, 686, 1074
Bme18I GGWCC 2 cut(s) 238, 811
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 4 cut(s) 238, 811, 1076, 1210
BmiI GGNNCC 5 cut(s) 207, 213, 240, 756, 870
BmrFI CCNGG 4 cut(s) 203, 536, 686, 1074
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 4 cut(s) 254, 793, 1083, 1111
BmtI GCTAGC 1 cut(s) 1437
BmuI ACTGGG 1 cut(s) 210
BpiI GAAGAC 4 cut(s) 105, 126, 309, 939
Bpu10I CCTNAGC 1 cut(s) 1335
Bpu1102I GCTNAGC 2 cut(s) 1199, 1341
Bpu14I TTCGAA 3 cut(s) 87, 96, 312
BpuEI CTTGAG 1 cut(s) 1233
BpuMI CCSGG 1 cut(s) 203
BsaAI YACGTR 1 cut(s) 817
BsaBI GATNNNNATC 2 cut(s) 393, 1068
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 5 cut(s) 534, 561, 685, 807, 1073
BsaWI WCCGGW 1 cut(s) 506
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 247, 277, 361, 391
Bsc4I CCNNNNNNNGG 7 cut(s) 208, 471, 535, 540, 541, 672, 911
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 2 cut(s) 216, 666
Bse3DI GCAATG 2 cut(s) 861, 1413
Bse8I GATNNNNATC 2 cut(s) 393, 1068
BseBI CCWGG 3 cut(s) 536, 686, 1074
BseDI CCNNGG 5 cut(s) 534, 561, 685, 807, 1073
BseGI GGATG 4 cut(s) 66, 154, 158, 1069
BseJI GATNNNNATC 2 cut(s) 393, 1068
BseLI CCNNNNNNNGG 7 cut(s) 208, 471, 535, 540, 541, 672, 911
BseMI GCAATG 2 cut(s) 861, 1413
BseMII CTCAG 3 cut(s) 942, 975, 1326
BseNI ACTGG 2 cut(s) 216, 666
BseRI GAGGAG 4 cut(s) 14, 121, 161, 1343
BseXI GCAGC 3 cut(s) 532, 668, 1231
BseYI CCCAGC 2 cut(s) 241, 968
BsgI GTGCAG 1 cut(s) 1371
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 9 cut(s) 185, 642, 698, 833, 845, 1078, 1136, 1157, 1211
BshNI GGYRCC 2 cut(s) 205, 754
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 1390
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 4 cut(s) 203, 209, 507, 912
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 7 cut(s) 208, 471, 535, 540, 541, 672, 911
BsmAI GTCTC 3 cut(s) 11, 381, 762
BsmBI CGTCTC 2 cut(s) 381, 762
BsmFI GGGAC 1 cut(s) 311
BsnI GGCC 9 cut(s) 185, 642, 698, 833, 845, 1078, 1136, 1157, 1211
Bso31I GGTCTC 1 cut(s) 11
BsoBI CYCGRG 1 cut(s) 428
Bsp119I TTCGAA 3 cut(s) 87, 96, 312
Bsp1286I GDGCHC 1 cut(s) 1390
Bsp1720I GCTNAGC 2 cut(s) 1199, 1341
Bsp19I CCATGG 1 cut(s) 807
BspACI CCGC 6 cut(s) 177, 344, 468, 673, 718, 846
BspANI GGCC 9 cut(s) 185, 642, 698, 833, 845, 1078, 1136, 1157, 1211
BspCNI CTCAG 3 cut(s) 943, 976, 1327
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 5 cut(s) 207, 213, 240, 756, 870
BspOI GCTAGC 1 cut(s) 1437
BspPI GGATC 5 cut(s) 206, 215, 219, 407, 1209
BspT104I TTCGAA 3 cut(s) 87, 96, 312
BspT107I GGYRCC 2 cut(s) 205, 754
BspTI CTTAAG 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 861, 1413
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 2 cut(s) 216, 666
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 5 cut(s) 534, 561, 685, 807, 1073
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 2 cut(s) 561, 807
Bst2UI CCWGG 3 cut(s) 536, 686, 1074
Bst4CI ACNGT 2 cut(s) 355, 454
Bst6I CTCTTC 2 cut(s) 38, 739
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 710
BstAPI GCANNNNNTGC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 817
BstBI TTCGAA 3 cut(s) 87, 96, 312
BstC8I GCNNGC 4 cut(s) 466, 913, 1386, 1435
BstDEI CTNAG 6 cut(s) 951, 984, 1199, 1335, 1341, 1346
BstDSI CCRYGG 1 cut(s) 807
BstF5I GGATG 4 cut(s) 66, 154, 158, 1069
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 516
BstMAI GTCTC 3 cut(s) 11, 381, 762
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 4 cut(s) 545, 554, 851, 1253
BstNI CCWGG 3 cut(s) 536, 686, 1074
BstSCI CCNGG 4 cut(s) 201, 534, 684, 1072
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 532, 668, 1231
BstV2I GAAGAC 4 cut(s) 105, 126, 309, 939
BstX2I RGATCY 2 cut(s) 211, 443
BstXI CCANNNNNNTGG 1 cut(s) 964
BstYI RGATCY 2 cut(s) 211, 443
BsuRI GGCC 9 cut(s) 185, 642, 698, 833, 845, 1078, 1136, 1157, 1211
BtgI CCRYGG 1 cut(s) 807
BtgZI GCGATG 1 cut(s) 627
BtsCI GGATG 4 cut(s) 66, 154, 158, 1069
BtsIMutI CAGTG 4 cut(s) 223, 357, 673, 981
Cac8I GCNNGC 4 cut(s) 466, 913, 1386, 1435
CaiI CAGNNNCTG 2 cut(s) 359, 691
CfoI GCGC 1 cut(s) 516
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 4 cut(s) 238, 811, 1076, 1210
CseI GACGC 1 cut(s) 110
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 6 cut(s) 517, 540, 793, 808, 1045, 1132
DdeI CTNAG 6 cut(s) 951, 984, 1199, 1335, 1341, 1346
EaeI YGGCCR 4 cut(s) 183, 696, 831, 1134
Eam1104I CTCTTC 2 cut(s) 38, 739
EarI CTCTTC 2 cut(s) 38, 739
EciI GGCGGA 1 cut(s) 688
Eco130I CCWWGG 2 cut(s) 561, 807
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 112
Eco47I GGWCC 2 cut(s) 238, 811
Eco57I CTGAAG 1 cut(s) 571
Eco72I CACGTG 1 cut(s) 817
Eco88I CYCGRG 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 1076
EcoRII CCWGG 3 cut(s) 534, 684, 1072
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 561, 807
EcoT22I ATGCAT 1 cut(s) 1056
ErhI CCWWGG 2 cut(s) 561, 807
Esp3I CGTCTC 2 cut(s) 381, 762
FaeI CATG 6 cut(s) 520, 543, 796, 811, 1048, 1135
FaqI GGGAC 1 cut(s) 311
FatI CATG 6 cut(s) 516, 539, 792, 807, 1044, 1131
FbaI TGATCA 1 cut(s) 997
FblI GTMKAC 1 cut(s) 319
Fnu4HI GCNGC 5 cut(s) 546, 682, 718, 846, 1245
FokI GGATG 4 cut(s) 53, 141, 145, 1056
Fsp4HI GCNGC 5 cut(s) 546, 682, 718, 846, 1245
FspBI CTAG 2 cut(s) 1278, 1434
GlaI GCGC 1 cut(s) 515
GluI GCNGC 5 cut(s) 546, 682, 718, 846, 1245
GsaI CCCAGC 2 cut(s) 245, 972
HaeIII GGCC 9 cut(s) 185, 642, 698, 833, 845, 1078, 1136, 1157, 1211
HapII CCGG 4 cut(s) 203, 209, 507, 912
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 516
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 6 cut(s) 520, 543, 796, 811, 1048, 1135
Hin6I GCGC 1 cut(s) 514
HinP1I GCGC 1 cut(s) 514
HindIII AAGCTT 3 cut(s) 1017, 1287, 1343
HinfI GANTC 9 cut(s) 77, 128, 233, 260, 383, 389, 617, 953, 1144
HpaII CCGG 4 cut(s) 203, 209, 507, 912
HphI GGTGA 5 cut(s) 136, 166, 496, 612, 1022
Hpy166II GTNNAC 2 cut(s) 320, 790
Hpy188I TCNGA 9 cut(s) 82, 238, 388, 394, 399, 417, 952, 1026, 1063
Hpy188III TCNNGA 8 cut(s) 230, 380, 441, 478, 631, 872, 995, 1250
Hpy8I GTNNAC 2 cut(s) 320, 790
Hpy99I CGWCG 3 cut(s) 110, 119, 184
HpyCH4III ACNGT 2 cut(s) 355, 454
HpyCH4IV ACGT 2 cut(s) 648, 816
HpyCH4V TGCA 6 cut(s) 859, 880, 1054, 1112, 1294, 1352
HpyF10VI GCNNNNNNNGC 4 cut(s) 545, 554, 851, 1253
HpyF3I CTNAG 6 cut(s) 951, 984, 1199, 1335, 1341, 1346
HpySE526I ACGT 2 cut(s) 648, 816
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 6 cut(s) 520, 543, 796, 811, 1048, 1135
HspAI GCGC 1 cut(s) 514
KroI GCCGGC 1 cut(s) 911
KroNI GCCGGC 1 cut(s) 913
Ksp22I TGATCA 1 cut(s) 997
LmnI GCTCC 2 cut(s) 706, 1381
Lsp1109I GCAGC 3 cut(s) 532, 668, 1231
LweI GCATC 4 cut(s) 254, 793, 1083, 1111
MaeI CTAG 2 cut(s) 1278, 1434
MaeII ACGT 2 cut(s) 648, 816
MaeIII GTNAC 2 cut(s) 355, 1190
MflI RGATCY 2 cut(s) 211, 443
MhlI GDGCHC 1 cut(s) 1390
MlsI TGGCCA 2 cut(s) 698, 833
MluCI AATT 6 cut(s) 496, 551, 656, 745, 1028, 1319
MluNI TGGCCA 2 cut(s) 698, 833
MlyI GAGTC 5 cut(s) 71, 122, 611, 962, 1138
MmeI TCCRAC 1 cut(s) 105
Mox20I TGGCCA 2 cut(s) 698, 833
Mph1103I ATGCAT 1 cut(s) 1056
MroNI GCCGGC 1 cut(s) 911
MroXI GAANNNNTTC 1 cut(s) 650
MscI TGGCCA 2 cut(s) 698, 833
MseI TTAA 2 cut(s) 711, 977
MslI CAYNNNNRTG 1 cut(s) 1049
Msp20I TGGCCA 2 cut(s) 698, 833
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 4 cut(s) 203, 209, 507, 912
MspR9I CCNGG 4 cut(s) 203, 536, 686, 1074
MvaI CCWGG 3 cut(s) 536, 686, 1074
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 4 cut(s) 545, 554, 851, 1253
NaeI GCCGGC 1 cut(s) 913
NciI CCSGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 807
NgoMIV GCCGGC 1 cut(s) 911
NheI GCTAGC 1 cut(s) 1433
NlaIII CATG 6 cut(s) 520, 543, 796, 811, 1048, 1135
NlaIV GGNNCC 5 cut(s) 207, 213, 240, 756, 870
NmuCI GTSAC 1 cut(s) 355
NsiI ATGCAT 1 cut(s) 1056
NspV TTCGAA 3 cut(s) 87, 96, 312
PcsI WCGNNNNNNNCGW 5 cut(s) 78, 93, 102, 114, 413
PdiI GCCGGC 1 cut(s) 913
PdmI GAANNNNTTC 1 cut(s) 650
PfeI GAWTC 4 cut(s) 233, 260, 383, 389
PkrI GCNGC 5 cut(s) 547, 683, 719, 847, 1246
PleI GAGTC 5 cut(s) 71, 122, 611, 961, 1138
PmaCI CACGTG 1 cut(s) 817
PmlI CACGTG 1 cut(s) 817
PpsI GAGTC 5 cut(s) 71, 122, 611, 961, 1138
Ppu21I YACGTR 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 534, 684, 1072
PspCI CACGTG 1 cut(s) 817
PspFI CCCAGC 2 cut(s) 241, 968
PspGI CCWGG 3 cut(s) 534, 684, 1072
PspN4I GGNNCC 5 cut(s) 207, 213, 240, 756, 870
PspPI GGNCC 4 cut(s) 238, 811, 1076, 1210
PstNI CAGNNNCTG 2 cut(s) 359, 691
PsuI RGATCY 2 cut(s) 211, 443
RseI CAYNNNNRTG 1 cut(s) 1049
SaqAI TTAA 2 cut(s) 711, 977
SatI GCNGC 5 cut(s) 546, 682, 718, 846, 1245
Sau96I GGNCC 4 cut(s) 238, 811, 1076, 1210
SchI GAGTC 5 cut(s) 71, 122, 611, 962, 1138
ScrFI CCNGG 4 cut(s) 203, 536, 686, 1074
SduI GDGCHC 1 cut(s) 1390
SfaNI GCATC 4 cut(s) 254, 793, 1083, 1111
SfuI TTCGAA 3 cut(s) 87, 96, 312
SinI GGWCC 2 cut(s) 238, 811
SmiMI CAYNNNNRTG 1 cut(s) 1049
SmlI CTYRAG 2 cut(s) 710, 1248
SmoI CTYRAG 2 cut(s) 710, 1248
Sse9I AATT 6 cut(s) 496, 551, 656, 745, 1028, 1319
SsiI CCGC 6 cut(s) 177, 344, 468, 673, 718, 846
SspMI CTAG 2 cut(s) 1278, 1434
StyD4I CCNGG 4 cut(s) 201, 534, 684, 1072
StyI CCWWGG 2 cut(s) 561, 807
TaaI ACNGT 2 cut(s) 355, 454
TaiI ACGT 2 cut(s) 651, 819
TaqI TCGA 7 cut(s) 87, 96, 114, 312, 407, 576, 783
TaqII GACCGA 1 cut(s) 425
TasI AATT 6 cut(s) 496, 551, 656, 745, 1028, 1319
TauI GCSGC 2 cut(s) 720, 848
TfiI GAWTC 4 cut(s) 233, 260, 383, 389
Tru1I TTAA 2 cut(s) 711, 977
Tru9I TTAA 2 cut(s) 711, 977
TscAI CASTG 4 cut(s) 223, 364, 673, 988
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 3 cut(s) 545, 681, 1244
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 6 cut(s) 478, 533, 809, 1033, 1412, 1433
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 4 cut(s) 223, 364, 673, 988
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 238, 811
XapI RAATTY 3 cut(s) 551, 656, 1028
XcmI CCANNNNNNNNNTGG 2 cut(s) 745, 814
XmiI GTMKAC 1 cut(s) 319
XmnI GAANNNNTTC 1 cut(s) 650
XspI CTAG 2 cut(s) 1278, 1434
Zsp2I ATGCAT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.