AT5G50590

hydroxysteroid dehydrogenase (HSD). Genes that encode HSD include At5g50600 and At5g50700 (HSD1), At3g47350(HSD2), At3g47360(HSD3), At5g50590 and At5g50690(HSD4), At5g50770(HSD6) (Plant Cell Physiology 50 1463). Two copies of HSD1 and HSD4 exist due to a gene duplication event. In Plant Physiology 145 87, At5g50690 is HSD7, At4g10020 is HSD5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008986
Physical Location & Seq
Forward (+)
20587936 .. 20589490
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50590.2

Sequence Viewer

Length: 900 bp
ATGGATTTAAACAACAAGATCTTTAACATCCTTCTTCCTATTGTCACGGTTTCGTTTCTTTTAGTGTTCATGCCATTTTCAATATTCTTCAAGCTCCTCCAGTTCATAAGGGGATGCAAAGAGTCCGAGAAAGTCAACGGAAAAGTCGTCATCATCACCGGATCTTCCTCAGGAATTGGCGAGCACCTAGCGTATGAGTATGCTCGTAGAGGAGCATACTTGACTCTAGTAGCTCGGAGAGAGGATCGTCTCCAGGTGGTGGCTGACCGGTGCAGGAAGCTTGGTTCACCAGACGTTGCTGTGGTACGCGGTGATGTCTCAGTCATCAAAGACTGTAAACGTTTTGTTCAAGAAACCATCTCTCGGTTTGGAAGATTGGATCACTTGGTGAACAATGCTGGAATTGCGGAGGCCAAATTTTTCGAGGACTATTCTGAAATTTCGGATGTTCTTCCCATTGTGAACACTAACTTTTGGGGACCGGTTTACGCGACACATTTCGCTATACCGCATCTCAAGAAAACAAAAGGAAAGATCATAGCGGTTGCATCACCTGCTGGATGGTCTGGAGTACCAAGGATGAGCATATATGCTGCAAGCAAAGCAGCTATGATAAACTTCTACGAGACTCTTAGGATCGAGCTTCATCCAGAAGTTGGTGTGACAATCGTGTTTCCTGGTCTAATCGAAAACGGAAATACTAATCCGGACCTTTTAGCTGAGAAACAAGACTGGTCGCAAGTTGTGACCATCGAGTCAGCAGCGGAATGCGCCAAAGCGGTAGTTAACGGAATCTGCAGAGGCAAAACCTTTGTGGCAGAGCCGTCGTGGGTTCGTGTCCTGTTCTGGCTTAGTGCTATATGCCCTGAGCTTCTGATCTCTAAGCCTAAAAGGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short

Protein Analysis

299

Amino Acids

33.25

Weight (kDa)

9.07

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 48 - 232 4.2e-45 short chain dehydrogenase
KR PF08659 50 - 212 1.4e-08 KR domain
adh_short_C2 PF13561 56 - 231 5.7e-28 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008986)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47350 AT3G47350 AT3G47350 AT3G47360 AT5G50590 AT5G50590 AT5G50690 AT5G50690
fragaria_vesca FvH4_5g08710
malus_domestica MD14G1152900.v1.1
prunus_persica Prupe.5G148000_v2.0.a1
pyrus_communis pycom14g12740
rosa_chinensis RchiOBHm_Chr7g0189561
rosa_laevigata RLG00000004600
rosa_multiflora Rmu_sc0001924.1_g000013
rosa_roxburghii Rroxscaffold_3G00265300
rosa_rugosa Rorug06G0499000
rosa_samantha Rh7AG104000 Rh7BG106700 Rh7CG108800 Rh7DG106100
rosa_wichuraiana Rw7G008980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 562
AasI GACNNNNNNGTC 1 cut(s) 754
Acc36I ACCTGC 1 cut(s) 562
AccB7I CCANNNNNTGG 2 cut(s) 259, 656
AccII CGCG 2 cut(s) 309, 491
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 6 cut(s) 309, 407, 509, 542, 764, 779
AclI AACGTT 1 cut(s) 340
AclWI GGATC 4 cut(s) 169, 252, 387, 644
AcsI RAATTY 2 cut(s) 416, 438
AdeI CACNNNGTG 1 cut(s) 388
AfaI GTAC 2 cut(s) 306, 573
AfiI CCNNNNNNNGG 3 cut(s) 259, 363, 656
AgeI ACCGGT 2 cut(s) 267, 481
AgsI TTSAA 3 cut(s) 81, 91, 350
AjnI CCWGG 2 cut(s) 252, 676
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
AluBI AGCT 7 cut(s) 94, 233, 280, 608, 643, 719, 871
AluI AGCT 7 cut(s) 94, 233, 280, 608, 643, 719, 871
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 3 cut(s) 254, 322, 620
AlwI GGATC 4 cut(s) 169, 252, 387, 644
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 1 cut(s) 411
ApeKI GCWGC 3 cut(s) 593, 605, 761
ApoI RAATTY 2 cut(s) 416, 438
AsiGI ACCGGT 2 cut(s) 267, 481
AspLEI GCGC 1 cut(s) 773
AspS9I GGNCC 2 cut(s) 479, 709
AsuHPI GGTGA 5 cut(s) 148, 279, 323, 400, 543
AvaII GGWCC 2 cut(s) 479, 709
AxyI CCTNAGG 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 3 cut(s) 580, 617, 773
BccI CCATC 3 cut(s) 365, 555, 758
BceAI ACGGC 1 cut(s) 808
BcgI CGANNNNNNTGC 2 cut(s) 807, 841
BciT130I CCWGG 2 cut(s) 254, 678
BcoDI GTCTC 3 cut(s) 254, 322, 620
BfaI CTAG 2 cut(s) 188, 227
BfmI CTRYAG 1 cut(s) 796
BfuAI ACCTGC 1 cut(s) 562
BglII AGATCT 1 cut(s) 18
BisI GCNGC 3 cut(s) 594, 606, 762
BlsI GCNGC 3 cut(s) 595, 607, 763
Bme1390I CCNGG 2 cut(s) 254, 678
Bme18I GGWCC 2 cut(s) 479, 709
BmgT120I GGNCC 2 cut(s) 479, 709
BmiI GGNNCC 1 cut(s) 480
BmrFI CCNGG 2 cut(s) 254, 678
BmsI GCATC 3 cut(s) 104, 520, 557
BpmI CTGGAG 3 cut(s) 83, 236, 588
Bpu10I CCTNAGC 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 500
BsaJI CCNNGG 1 cut(s) 575
BsaWI WCCGGW 4 cut(s) 158, 267, 481, 706
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 363, 656
Bse118I RCCGGY 2 cut(s) 267, 481
Bse1I ACTGG 2 cut(s) 100, 737
Bse21I CCTNAGG 1 cut(s) 169
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 2 cut(s) 254, 678
BseDI CCNNGG 1 cut(s) 575
BseGI GGATG 6 cut(s) 27, 119, 451, 566, 585, 646
BseLI CCNNNNNNNGG 3 cut(s) 259, 363, 656
BseMII CTCAG 4 cut(s) 183, 333, 711, 858
BseNI ACTGG 2 cut(s) 100, 737
BseRI GAGGAG 2 cut(s) 86, 225
BseXI GCAGC 3 cut(s) 580, 617, 773
BsgI GTGCAG 1 cut(s) 292
Bsh1236I CGCG 2 cut(s) 309, 491
BshFI GGCC 1 cut(s) 413
BshTI ACCGGT 2 cut(s) 267, 481
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 4 cut(s) 159, 268, 482, 707
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 3 cut(s) 259, 363, 656
BsmAI GTCTC 3 cut(s) 254, 322, 620
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 773
BsnI GGCC 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 186
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
BspACI CCGC 6 cut(s) 309, 407, 509, 542, 764, 779
BspANI GGCC 1 cut(s) 413
BspCNI CTCAG 4 cut(s) 182, 332, 712, 859
BspEI TCCGGA 1 cut(s) 706
BspFNI CGCG 2 cut(s) 309, 491
BspLI GGNNCC 1 cut(s) 480
BspMAI CTGCAG 1 cut(s) 800
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 4 cut(s) 169, 252, 387, 644
BsrFI RCCGGY 2 cut(s) 267, 481
BsrI ACTGG 2 cut(s) 100, 737
BssAI RCCGGY 2 cut(s) 267, 481
BssECI CCNNGG 1 cut(s) 575
BssMI GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
BssT1I CCWWGG 1 cut(s) 575
Bst2UI CCWGG 2 cut(s) 254, 678
Bst4CI ACNGT 2 cut(s) 49, 335
BstAPI GCANNNNNTGC 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 182, 598
BstDEI CTNAG 7 cut(s) 169, 319, 632, 720, 851, 867, 882
BstF5I GGATG 6 cut(s) 27, 119, 451, 566, 585, 646
BstFNI CGCG 2 cut(s) 309, 491
BstHHI GCGC 1 cut(s) 773
BstKTI GATC 7 cut(s) 21, 164, 247, 382, 537, 639, 879
BstMAI GTCTC 3 cut(s) 254, 322, 620
BstMBI GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
BstMWI GCNNNNNNNGC 4 cut(s) 404, 554, 602, 770
BstNI CCWGG 2 cut(s) 254, 678
BstSCI CCNGG 2 cut(s) 252, 676
BstSFI CTRYAG 1 cut(s) 796
BstUI CGCG 2 cut(s) 309, 491
BstV1I GCAGC 3 cut(s) 580, 617, 773
BstX2I RGATCY 2 cut(s) 18, 161
BstYI RGATCY 2 cut(s) 18, 161
Bsu36I CCTNAGG 1 cut(s) 169
BsuRI GGCC 1 cut(s) 413
BtsCI GGATG 6 cut(s) 27, 119, 451, 566, 585, 646
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 2 cut(s) 182, 598
CfoI GCGC 1 cut(s) 773
Cfr10I RCCGGY 2 cut(s) 267, 481
Cfr13I GGNCC 2 cut(s) 479, 709
Csp6I GTAC 2 cut(s) 305, 572
CspAI ACCGGT 2 cut(s) 267, 481
CviAII CATG 1 cut(s) 70
CviQI GTAC 2 cut(s) 305, 572
DdeI CTNAG 7 cut(s) 169, 319, 632, 720, 851, 867, 882
DpnI GATC 7 cut(s) 20, 163, 246, 381, 536, 638, 878
DpnII GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
DraI TTTAAA 1 cut(s) 9
DraIII CACNNNGTG 1 cut(s) 388
DrdI GACNNNNNNGTC 1 cut(s) 754
DseDI GACNNNNNNGTC 1 cut(s) 754
Eco130I CCWWGG 1 cut(s) 575
Eco47I GGWCC 2 cut(s) 479, 709
Eco81I CCTNAGG 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 252, 676
EcoT14I CCWWGG 1 cut(s) 575
ErhI CCWWGG 1 cut(s) 575
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 1 cut(s) 73
FaqI GGGAC 1 cut(s) 492
FatI CATG 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 594, 606, 762
FokI GGATG 6 cut(s) 14, 126, 458, 573, 592, 633
Fsp4HI GCNGC 3 cut(s) 594, 606, 762
FspBI CTAG 2 cut(s) 188, 227
GlaI GCGC 1 cut(s) 772
GluI GCNGC 3 cut(s) 594, 606, 762
GsuI CTGGAG 3 cut(s) 83, 236, 588
HaeIII GGCC 1 cut(s) 413
HapII CCGG 4 cut(s) 159, 268, 482, 707
HhaI GCGC 1 cut(s) 773
Hin1II CATG 1 cut(s) 73
Hin6I GCGC 1 cut(s) 771
HinP1I GCGC 1 cut(s) 771
HincII GTYRAC 2 cut(s) 136, 787
HindII GTYRAC 2 cut(s) 136, 787
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 5 cut(s) 122, 223, 628, 755, 792
HpaI GTTAAC 1 cut(s) 787
HpaII CCGG 4 cut(s) 159, 268, 482, 707
HphI GGTGA 5 cut(s) 148, 279, 323, 400, 543
Hpy166II GTNNAC 7 cut(s) 136, 287, 338, 391, 463, 487, 787
Hpy188I TCNGA 5 cut(s) 127, 237, 436, 445, 876
Hpy188III TCNNGA 6 cut(s) 171, 350, 517, 567, 650, 707
Hpy8I GTNNAC 7 cut(s) 136, 287, 338, 391, 463, 487, 787
Hpy99I CGWCG 1 cut(s) 829
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 2 cut(s) 49, 335
HpyCH4IV ACGT 2 cut(s) 294, 340
HpyCH4V TGCA 5 cut(s) 117, 273, 548, 596, 798
HpyF10VI GCNNNNNNNGC 4 cut(s) 404, 554, 602, 770
HpyF3I CTNAG 7 cut(s) 169, 319, 632, 720, 851, 867, 882
HpySE526I ACGT 2 cut(s) 294, 340
Hsp92II CATG 1 cut(s) 73
HspAI GCGC 1 cut(s) 771
Kpn2I TCCGGA 1 cut(s) 706
KspAI GTTAAC 1 cut(s) 787
Kzo9I GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
LmnI GCTCC 2 cut(s) 99, 212
Lsp1109I GCAGC 3 cut(s) 580, 617, 773
LweI GCATC 3 cut(s) 104, 520, 557
MaeI CTAG 2 cut(s) 188, 227
MaeII ACGT 2 cut(s) 294, 340
MaeIII GTNAC 3 cut(s) 43, 661, 745
MalI GATC 7 cut(s) 20, 163, 246, 381, 536, 638, 878
MboI GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
MboII GAAGA 5 cut(s) 26, 79, 156, 384, 443
MflI RGATCY 2 cut(s) 18, 161
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 5 cut(s) 174, 402, 416, 438, 895
MlyI GAGTC 4 cut(s) 131, 217, 622, 764
MnlI CCTC 7 cut(s) 107, 178, 203, 235, 403, 418, 794
MroI TCCGGA 1 cut(s) 706
MseI TTAA 4 cut(s) 8, 24, 786, 898
MspA1I CMGCKG 1 cut(s) 764
MspI CCGG 4 cut(s) 159, 268, 482, 707
MspR9I CCNGG 2 cut(s) 254, 678
Mva1269I GAATGC 1 cut(s) 773
MvaI CCWGG 2 cut(s) 254, 678
MvnI CGCG 2 cut(s) 309, 491
MwoI GCNNNNNNNGC 4 cut(s) 404, 554, 602, 770
NdeII GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
NlaIII CATG 1 cut(s) 73
NlaIV GGNNCC 1 cut(s) 480
NmuCI GTSAC 3 cut(s) 43, 661, 745
PaqCI CACCTGC 1 cut(s) 562
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 773
PfeI GAWTC 1 cut(s) 792
PflMI CCANNNNNTGG 2 cut(s) 259, 656
PinAI ACCGGT 2 cut(s) 267, 481
PkrI GCNGC 3 cut(s) 595, 607, 763
PleI GAGTC 4 cut(s) 130, 217, 622, 763
PpsI GAGTC 4 cut(s) 130, 217, 622, 763
Psp1406I AACGTT 1 cut(s) 340
Psp6I CCWGG 2 cut(s) 252, 676
PspGI CCWGG 2 cut(s) 252, 676
PspN4I GGNNCC 1 cut(s) 480
PspPI GGNCC 2 cut(s) 479, 709
PstI CTGCAG 1 cut(s) 800
PsuI RGATCY 2 cut(s) 18, 161
RsaI GTAC 2 cut(s) 306, 573
RsaNI GTAC 2 cut(s) 305, 572
SaqAI TTAA 4 cut(s) 8, 24, 786, 898
SatI GCNGC 3 cut(s) 594, 606, 762
Sau3AI GATC 7 cut(s) 18, 161, 244, 379, 534, 636, 876
Sau96I GGNCC 2 cut(s) 479, 709
SchI GAGTC 4 cut(s) 131, 217, 622, 764
ScrFI CCNGG 2 cut(s) 254, 678
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 3 cut(s) 104, 520, 557
SfcI CTRYAG 1 cut(s) 796
SinI GGWCC 2 cut(s) 479, 709
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 5 cut(s) 174, 402, 416, 438, 895
SsiI CCGC 6 cut(s) 309, 407, 509, 542, 764, 779
SspI AATATT 1 cut(s) 84
SspMI CTAG 2 cut(s) 188, 227
StyD4I CCNGG 2 cut(s) 252, 676
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 2 cut(s) 49, 335
TaiI ACGT 2 cut(s) 297, 343
TaqI TCGA 4 cut(s) 423, 639, 687, 753
TasI AATT 5 cut(s) 174, 402, 416, 438, 895
TfiI GAWTC 1 cut(s) 792
Tru1I TTAA 4 cut(s) 8, 24, 786, 898
Tru9I TTAA 4 cut(s) 8, 24, 786, 898
TseFI GTSAC 3 cut(s) 43, 661, 745
TseI GCWGC 3 cut(s) 593, 605, 761
Tsp45I GTSAC 3 cut(s) 43, 661, 745
TspDTI ATGAA 3 cut(s) 58, 94, 635
TspGWI ACGGA 3 cut(s) 153, 708, 804
Van91I CCANNNNNTGG 2 cut(s) 259, 656
VpaK11BI GGWCC 2 cut(s) 479, 709
XapI RAATTY 2 cut(s) 416, 438
XspI CTAG 2 cut(s) 188, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.