AT5G51210

Oleosin 18.5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009856
Physical Location & Seq
Forward (+)
20820068 .. 20820944
877 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51210.1

Sequence Viewer

Length: 426 bp
ATGGCGGACCAAACAAGAACCCATCACGAGATGATAAGCCGAGACAGTACCCAAGAGGCCCATCCGAAGGCCAGGCAGATGGTGAAGGCAGCAACCGCTGTCACAGCCGGTGGATCCCTACTTGTCCTCTCCGGCTTAACACTCGCTGGAACAGTCATCGCACTCACGGTGGCTACTCCTCTCCTCGTCATCTTCAGCCCCGTCTTGGTTCCAGCAGTGGTAACCGTTGCTCTCATCATTACCGGATTCCTTGCATCCGGTGGCTTTGGAATAGCCGCCATTACCGCCTTCTCTTGGCTCTACAGGCACATGACGGGATCTGGATCGGATAAGATAGAGAATGCTCGGATGAAGGTTGGAAGCAGAGTGCAGGATACTAAGTATGGGCAGCACAACATTGGAGTCCAACACCAACAAGTTTCTTAA

Protein Analysis

141

Amino Acids

14.85

Weight (kDa)

9.86

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 25 - 127 2.2e-39 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 96, 276, 285
AclWI GGATC 4 cut(s) 108, 121, 325, 331
AcuI CTGAAG 1 cut(s) 178
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 3 cut(s) 67, 205, 294
AjnI CCWGG 1 cut(s) 71
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 4 cut(s) 108, 121, 325, 331
AoxI GGCC 2 cut(s) 57, 69
ApeKI GCWGC 2 cut(s) 89, 388
AspS9I GGNCC 2 cut(s) 7, 58
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 7
BamHI GGATCC 1 cut(s) 113
BauI CACGAG 1 cut(s) 26
BbvI GCAGC 2 cut(s) 101, 400
BccI CCATC 3 cut(s) 30, 69, 73
BciT130I CCWGG 1 cut(s) 73
BciVI GTATCC 1 cut(s) 367
BcoDI GTCTC 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 301
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 3 cut(s) 90, 276, 389
BlsI GCNGC 3 cut(s) 91, 277, 390
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 7, 58
BmiI GGNNCC 2 cut(s) 115, 210
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 1 cut(s) 263
BsaBI GATNNNNATC 1 cut(s) 322
BsaWI WCCGGW 2 cut(s) 242, 257
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 205, 294
Bse118I RCCGGY 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 322
BseBI CCWGG 1 cut(s) 73
BseGI GGATG 3 cut(s) 61, 254, 354
BseJI GATNNNNATC 1 cut(s) 322
BseLI CCNNNNNNNGG 3 cut(s) 67, 205, 294
BseRI GAGGAG 2 cut(s) 168, 173
BseXI GCAGC 2 cut(s) 101, 400
BsgI GTGCAG 1 cut(s) 389
BshFI GGCC 2 cut(s) 59, 71
BsiSI CCGG 4 cut(s) 108, 132, 243, 258
BslI CCNNNNNNNGG 3 cut(s) 67, 205, 294
BsmAI GTCTC 1 cut(s) 36
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 2 cut(s) 59, 71
Bsp143I GATC 3 cut(s) 113, 317, 323
BspACI CCGC 4 cut(s) 5, 96, 276, 285
BspANI GGCC 2 cut(s) 59, 71
BspLI GGNNCC 2 cut(s) 115, 210
BspPI GGATC 4 cut(s) 108, 121, 325, 331
BsrFI RCCGGY 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 107
BssMI GATC 3 cut(s) 113, 317, 323
BssSI CACGAG 1 cut(s) 26
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 4 cut(s) 47, 154, 169, 226
BstDEI CTNAG 1 cut(s) 378
BstEII GGTNACC 1 cut(s) 220
BstF5I GGATG 3 cut(s) 61, 254, 354
BstKTI GATC 3 cut(s) 116, 320, 326
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 3 cut(s) 113, 317, 323
BstMWI GCNNNNNNNGC 4 cut(s) 95, 104, 284, 304
BstNI CCWGG 1 cut(s) 73
BstPI GGTNACC 1 cut(s) 220
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 301
BstV1I GCAGC 2 cut(s) 101, 400
BstX2I RGATCY 2 cut(s) 113, 317
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 2 cut(s) 113, 317
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 2 cut(s) 59, 71
BtgZI GCGATG 1 cut(s) 142
BtsCI GGATG 3 cut(s) 61, 254, 354
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 222
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 7, 58
Csp6I GTAC 1 cut(s) 48
CviAII CATG 1 cut(s) 310
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 378
DpnI GATC 3 cut(s) 115, 319, 325
DpnII GATC 3 cut(s) 113, 317, 323
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 178
Eco91I GGTNACC 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 71
FaeI CATG 1 cut(s) 313
FaiI YATR 2 cut(s) 311, 384
FatI CATG 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 90, 276, 389
FokI GGATG 3 cut(s) 48, 241, 361
Fsp4HI GCNGC 3 cut(s) 90, 276, 389
GluI GCNGC 3 cut(s) 90, 276, 389
HaeIII GGCC 2 cut(s) 59, 71
HapII CCGG 4 cut(s) 108, 132, 243, 258
Hin1II CATG 1 cut(s) 313
HinfI GANTC 2 cut(s) 246, 402
HpaII CCGG 4 cut(s) 108, 132, 243, 258
HphI GGTGA 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 66, 328, 348
Hpy188III TCNNGA 2 cut(s) 26, 321
HpyAV CCTTC 4 cut(s) 61, 79, 298, 346
HpyCH4III ACNGT 4 cut(s) 47, 154, 169, 226
HpyCH4V TGCA 2 cut(s) 254, 370
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 104, 284, 304
HpyF3I CTNAG 1 cut(s) 378
Hsp92II CATG 1 cut(s) 313
Kzo9I GATC 3 cut(s) 113, 317, 323
Lsp1109I GCAGC 2 cut(s) 101, 400
LweI GCATC 1 cut(s) 263
MaeIII GTNAC 2 cut(s) 100, 220
MalI GATC 3 cut(s) 115, 319, 325
MboI GATC 3 cut(s) 113, 317, 323
MboII GAAGA 1 cut(s) 184
MflI RGATCY 2 cut(s) 113, 317
MlyI GAGTC 1 cut(s) 411
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 4 cut(s) 49, 137, 189, 194
MseI TTAA 2 cut(s) 137, 424
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 4 cut(s) 108, 132, 243, 258
MspR9I CCNGG 1 cut(s) 73
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 4 cut(s) 95, 104, 284, 304
NdeII GATC 3 cut(s) 113, 317, 323
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 2 cut(s) 115, 210
NmeAIII GCCGAG 1 cut(s) 65
NmuCI GTSAC 1 cut(s) 100
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 1 cut(s) 246
PkrI GCNGC 3 cut(s) 91, 277, 390
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 71
PspEI GGTNACC 1 cut(s) 220
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 115, 210
PspPI GGNCC 2 cut(s) 7, 58
PsuI RGATCY 2 cut(s) 113, 317
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SaqAI TTAA 2 cut(s) 137, 424
SatI GCNGC 3 cut(s) 90, 276, 389
Sau3AI GATC 3 cut(s) 113, 317, 323
Sau96I GGNCC 2 cut(s) 7, 58
SchI GAGTC 1 cut(s) 411
ScrFI CCNGG 1 cut(s) 73
SetI ASST 1 cut(s) 357
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 1 cut(s) 301
SinI GGWCC 1 cut(s) 7
SsiI CCGC 4 cut(s) 5, 96, 276, 285
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 4 cut(s) 47, 154, 169, 226
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 1 cut(s) 246
Tru1I TTAA 2 cut(s) 137, 424
Tru9I TTAA 2 cut(s) 137, 424
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 2 cut(s) 89, 388
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 365
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.