MD07G1248300.v1.1

Oleosin 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009856
Physical Location & Seq
Reverse (-)
31759572 .. 31760018
447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1248300.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCTGATACACAAGGCCACCAGCACTACCAGCAGTCCCTTCGTTTCCAAGGCGGCCAACAAGGCATGCAGCAGCAGCATCAGAAGTCCTACCAAGTCGCCAAGGCGGCCACAGCTGTGACCGCGGGTGGGTCCCTTTTGGTGCTCTCTGGGCTGGTCCTTGCCGGCACGGTCATAGCTTTGACCATAGCGACCCCCCTGCTTGTCATATTCAGTCCGGTTATTGTCCCGGCCGTGATCACCCTGTCCCTCATAACAATGGGGTTCTTGACCTCCGGCGGGTTTGGTGTGGCGGCGGTGACGGTGTTGTCGTGGATCTACCGGTACGTGACCGGGAGGCACCCACCTGACGCGGACCAGCTAGACTCGGCTCGTTACAAGCTGGCTGGGAAGGCGAGGGAGATGAGGGACAGGGCTGAGCAGTACGGTCAACAGCAGGCTTCTTAA

Protein Analysis

149

Amino Acids

15.84

Weight (kDa)

9.63

Isoelectric Point (pI)

27.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 32 - 143 1.1e-46 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
AccB1I GGYRCC 1 cut(s) 339
AccII CGCG 2 cut(s) 125, 353
AciI CCGC 8 cut(s) 54, 107, 123, 125, 279, 293, 296, 353
AclWI GGATC 1 cut(s) 323
AcoI YGGCCR 3 cut(s) 55, 108, 231
AfaI GTAC 2 cut(s) 326, 425
AfiI CCNNNNNNNGG 2 cut(s) 129, 279
AgeI ACCGGT 1 cut(s) 321
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 4 cut(s) 116, 179, 361, 382
AluI AGCT 4 cut(s) 116, 179, 361, 382
Alw21I GWGCWC 1 cut(s) 147
AlwI GGATC 1 cut(s) 323
AoxI GGCC 4 cut(s) 16, 55, 108, 231
ApeKI GCWGC 3 cut(s) 70, 73, 76
AsiGI ACCGGT 1 cut(s) 321
AspS9I GGNCC 3 cut(s) 132, 157, 355
AsuC2I CCSGG 2 cut(s) 230, 334
AsuHPI GGTGA 2 cut(s) 232, 310
AvaII GGWCC 3 cut(s) 132, 157, 355
BanI GGYRCC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 3 cut(s) 82, 85, 88
BceAI ACGGC 1 cut(s) 218
BcgI CGANNNNNNTGC 4 cut(s) 23, 57, 181, 215
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 2 cut(s) 230, 334
BfaI CTAG 1 cut(s) 362
BglI GCCNNNNNGGC 2 cut(s) 63, 107
BisI GCNGC 6 cut(s) 55, 71, 74, 77, 108, 294
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 6 cut(s) 56, 72, 75, 78, 109, 295
Bme1390I CCNGG 2 cut(s) 230, 334
Bme18I GGWCC 3 cut(s) 132, 157, 355
BmgT120I GGNCC 3 cut(s) 132, 157, 355
BmiI GGNNCC 3 cut(s) 133, 134, 341
BmrFI CCNGG 2 cut(s) 230, 334
BmsI GCATC 1 cut(s) 88
Bpu1102I GCTNAGC 1 cut(s) 417
BpuMI CCSGG 2 cut(s) 230, 334
BsaAI YACGTR 1 cut(s) 328
BsaJI CCNNGG 3 cut(s) 49, 102, 123
BsaWI WCCGGW 2 cut(s) 217, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 279
Bse118I RCCGGY 2 cut(s) 164, 321
BseDI CCNNGG 3 cut(s) 49, 102, 123
BseLI CCNNNNNNNGG 2 cut(s) 129, 279
BseMII CTCAG 1 cut(s) 408
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 82, 85, 88
BseYI CCCAGC 1 cut(s) 386
Bsh1236I CGCG 2 cut(s) 125, 353
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 4 cut(s) 18, 57, 110, 233
BshNI GGYRCC 1 cut(s) 339
BshTI ACCGGT 1 cut(s) 321
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 6 cut(s) 165, 218, 230, 276, 322, 333
BslFI GGGAC 5 cut(s) 22, 118, 212, 232, 422
BslI CCNNNNNNNGG 2 cut(s) 129, 279
BsmFI GGGAC 5 cut(s) 22, 118, 212, 232, 422
BsnI GGCC 4 cut(s) 18, 57, 110, 233
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 2 cut(s) 237, 315
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 8 cut(s) 54, 107, 123, 125, 279, 293, 296, 353
BspANI GGCC 4 cut(s) 18, 57, 110, 233
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 2 cut(s) 125, 353
BspLI GGNNCC 3 cut(s) 133, 134, 341
BspPI GGATC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 339
BsrFI RCCGGY 2 cut(s) 164, 321
BssAI RCCGGY 2 cut(s) 164, 321
BssECI CCNNGG 3 cut(s) 49, 102, 123
BssMI GATC 2 cut(s) 237, 315
BssT1I CCWWGG 2 cut(s) 49, 102
Bst4CI ACNGT 3 cut(s) 172, 304, 428
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 4 cut(s) 68, 166, 384, 438
BstDEI CTNAG 1 cut(s) 417
BstDSI CCRYGG 1 cut(s) 123
BstFNI CGCG 2 cut(s) 125, 353
BstKTI GATC 2 cut(s) 240, 318
BstMBI GATC 2 cut(s) 237, 315
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 8 cut(s) 31, 63, 76, 107, 113, 122, 151, 392
BstNSI RCATGY 1 cut(s) 70
BstSCI CCNGG 2 cut(s) 228, 332
BstUI CGCG 2 cut(s) 125, 353
BstV1I GCAGC 3 cut(s) 82, 85, 88
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BstZI CGGCCG 1 cut(s) 231
BsuRI GGCC 4 cut(s) 18, 57, 110, 233
BtgI CCRYGG 1 cut(s) 123
Cac8I GCNNGC 4 cut(s) 68, 166, 384, 438
Cfr10I RCCGGY 2 cut(s) 164, 321
Cfr13I GGNCC 3 cut(s) 132, 157, 355
Cfr42I CCGCGG 1 cut(s) 126
CseI GACGC 1 cut(s) 359
Csp6I GTAC 2 cut(s) 325, 424
CspAI ACCGGT 1 cut(s) 321
CviAII CATG 1 cut(s) 67
CviQI GTAC 2 cut(s) 325, 424
DdeI CTNAG 1 cut(s) 417
DpnI GATC 2 cut(s) 239, 317
DpnII GATC 2 cut(s) 237, 315
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 3 cut(s) 55, 108, 231
EagI CGGCCG 1 cut(s) 231
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 49, 102
Eco47I GGWCC 3 cut(s) 132, 157, 355
Eco52I CGGCCG 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 132
EcoT14I CCWWGG 2 cut(s) 49, 102
ErhI CCWWGG 2 cut(s) 49, 102
FaeI CATG 1 cut(s) 70
FaiI YATR 5 cut(s) 68, 176, 188, 209, 254
FaqI GGGAC 5 cut(s) 22, 118, 212, 232, 422
FatI CATG 1 cut(s) 66
FauI CCCGC 2 cut(s) 118, 272
FbaI TGATCA 1 cut(s) 237
Fnu4HI GCNGC 6 cut(s) 55, 71, 74, 77, 108, 294
Fsp4HI GCNGC 6 cut(s) 55, 71, 74, 77, 108, 294
FspBI CTAG 1 cut(s) 362
GluI GCNGC 6 cut(s) 55, 71, 74, 77, 108, 294
GsaI CCCAGC 1 cut(s) 390
HaeIII GGCC 4 cut(s) 18, 57, 110, 233
HapII CCGG 6 cut(s) 165, 218, 230, 276, 322, 333
HgaI GACGC 1 cut(s) 359
Hin1II CATG 1 cut(s) 70
HincII GTYRAC 1 cut(s) 431
HindII GTYRAC 1 cut(s) 431
HinfI GANTC 1 cut(s) 365
HpaII CCGG 6 cut(s) 165, 218, 230, 276, 322, 333
HphI GGTGA 2 cut(s) 232, 310
Hpy166II GTNNAC 1 cut(s) 431
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 1 cut(s) 268
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 2 cut(s) 50, 385
HpyCH4III ACNGT 3 cut(s) 172, 304, 428
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 63, 76, 107, 113, 122, 151, 392
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 1 cut(s) 70
KflI GGGWCCC 1 cut(s) 132
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 237
KspI CCGCGG 1 cut(s) 126
Kzo9I GATC 2 cut(s) 237, 315
Lsp1109I GCAGC 3 cut(s) 82, 85, 88
LweI GCATC 1 cut(s) 88
MaeI CTAG 1 cut(s) 362
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 4 cut(s) 118, 298, 328, 374
MalI GATC 2 cut(s) 239, 317
MboI GATC 2 cut(s) 237, 315
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 147
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 5 cut(s) 260, 283, 330, 390, 399
MroNI GCCGGC 1 cut(s) 164
MseI TTAA 1 cut(s) 445
MslI CAYNNNNRTG 2 cut(s) 116, 257
MspA1I CMGCKG 2 cut(s) 116, 125
MspI CCGG 6 cut(s) 165, 218, 230, 276, 322, 333
MspR9I CCNGG 2 cut(s) 230, 334
MvnI CGCG 2 cut(s) 125, 353
MwoI GCNNNNNNNGC 8 cut(s) 31, 63, 76, 107, 113, 122, 151, 392
NaeI GCCGGC 1 cut(s) 166
NciI CCSGG 2 cut(s) 230, 334
NdeII GATC 2 cut(s) 237, 315
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 3 cut(s) 133, 134, 341
NmeAIII GCCGAG 1 cut(s) 347
NmuCI GTSAC 3 cut(s) 118, 298, 328
NspI RCATGY 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 116
PaeI GCATGC 1 cut(s) 70
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PdiI GCCGGC 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 321
PkrI GCNGC 6 cut(s) 56, 72, 75, 78, 109, 295
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
Ppu21I YACGTR 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 386
PspN4I GGNNCC 3 cut(s) 133, 134, 341
PspPI GGNCC 3 cut(s) 132, 157, 355
PspPPI RGGWCCY 1 cut(s) 132
PsuI RGATCY 1 cut(s) 315
PvuII CAGCTG 1 cut(s) 116
RsaI GTAC 2 cut(s) 326, 425
RsaNI GTAC 2 cut(s) 325, 424
RseI CAYNNNNRTG 2 cut(s) 116, 257
SacII CCGCGG 1 cut(s) 126
SaqAI TTAA 1 cut(s) 445
SatI GCNGC 6 cut(s) 55, 71, 74, 77, 108, 294
Sau3AI GATC 2 cut(s) 237, 315
Sau96I GGNCC 3 cut(s) 132, 157, 355
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 2 cut(s) 230, 334
SduI GDGCHC 1 cut(s) 147
SetI ASST 7 cut(s) 118, 181, 275, 330, 349, 363, 384
SfaNI GCATC 1 cut(s) 88
Sfr303I CCGCGG 1 cut(s) 126
SgrBI CCGCGG 1 cut(s) 126
SinI GGWCC 3 cut(s) 132, 157, 355
SmiMI CAYNNNNRTG 2 cut(s) 116, 257
SphI GCATGC 1 cut(s) 70
SsiI CCGC 8 cut(s) 54, 107, 123, 125, 279, 293, 296, 353
SspMI CTAG 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 228, 332
StyI CCWWGG 2 cut(s) 49, 102
TaaI ACNGT 3 cut(s) 172, 304, 428
TaiI ACGT 1 cut(s) 330
TauI GCSGC 3 cut(s) 57, 110, 296
Tru1I TTAA 1 cut(s) 445
Tru9I TTAA 1 cut(s) 445
TseFI GTSAC 3 cut(s) 118, 298, 328
TseI GCWGC 3 cut(s) 70, 73, 76
Tsp45I GTSAC 3 cut(s) 118, 298, 328
VpaK11BI GGWCC 3 cut(s) 132, 157, 355
XceI RCATGY 1 cut(s) 70
XspI CTAG 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.