AT5G52240

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010724
Physical Location & Seq
Forward (+)
21212940 .. 21214842
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52240.1

Sequence Viewer

Length: 663 bp
ATGGCGTTAGAACTATGGCAAACTCTCAAAGAAGCAATCCATGCTTACACAGGTCTTTCTCCTGTTGTCTTCTTCACTGCTCTAGCTCTCGCCTTCGCCATTTACCAAGTCATCTCAGGCTGGTTTGCCTCGCCGTTCGATGATGTTAACCGACATCAGAGAGCTAGATCCTTGGCTCAAGAGGAGGAGCCACCGATTCCTCAGCCTGTTCAAGTCGGTGAGATCACGGAGGAGGAGCTTAAACAGTACGATGGCTCTGATCCTCAAAAGCCCCTTCTTATGGCTATCAAACATCAGATCTATGATGTTACACAAAGCAGGATGTTCTACGGACCAGGAGGACCATATGCTTTGTTTGCAGGGAAAGACGCTAGCCGAGCTCTTGCAAAGATGTCATTTGAGGAGAAAGACTTGACTTGGGATGTCTCTGGTCTTGGTCCCTTTGAGCTAGATGCTCTTCAAGATTGGGAGTACAAGTTCATGAGCAAGTATGCTAAGGTTGGTACTGTCAAAGTGGCTGGTTCAGAACCTGAAACCGCATCTGTCTCTGAACCCACAGAGAATGTTGAGCAAGATGCTCATGTAACCACAACGCCTGGGAAGACCGTTGTTGATAAGAGTGATGATGCTCCTGCTGAGACTGTGTTGAAGAAGGAGGAGTAG

Protein Analysis

220

Amino Acids

24.41

Weight (kDa)

4.67

Isoelectric Point (pI)

57.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 76 - 169 9.2e-12 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010724)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52240 AT5G52240
fragaria_vesca FvH4_7g29310
malus_domestica MD01G1199500.v1.1 MD06G1084900.v1.1 MD07G1267300.v1.1 MD13G1074400.v1.1 MD17G1239500.v1.1
prunus_persica Prupe.2G293000_v2.0.a1
pyrus_communis pycom01g20940
rosa_chinensis RchiOBHm_Chr1g0377051
rosa_laevigata RLG00000026547
rosa_multiflora Rmu_ssc0000155.1_g000020
rosa_roxburghii Rroxscaffold_4G00281470
rosa_rugosa Rorug01G0401300
rosa_samantha Rh1AG418400 Rh1BG377500 Rh1CG391500 Rh1DG408200
rosa_wichuraiana Rw1G036710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 537
AclWI GGATC 2 cut(s) 162, 254
AfaI GTAC 3 cut(s) 248, 473, 505
AfiI CCNNNNNNNGG 1 cut(s) 280
AgsI TTSAA 3 cut(s) 212, 461, 649
AjnI CCWGG 2 cut(s) 334, 595
AloI GAACNNNNNNTCC 2 cut(s) 461, 493
AluBI AGCT 5 cut(s) 86, 164, 238, 380, 448
AluI AGCT 5 cut(s) 86, 164, 238, 380, 448
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 3 cut(s) 430, 550, 632
AlwI GGATC 2 cut(s) 162, 254
AlwNI CAGNNNCTG 1 cut(s) 530
AspS9I GGNCC 3 cut(s) 332, 341, 437
AsuHPI GGTGA 1 cut(s) 230
AsuNHI GCTAGC 1 cut(s) 371
AvaII GGWCC 3 cut(s) 332, 341, 437
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 2 cut(s) 61, 608
Bbv12I GWGCWC 1 cut(s) 382
BbvCI CCTCAGC 1 cut(s) 201
BccI CCATC 1 cut(s) 245
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 2 cut(s) 336, 597
BcoDI GTCTC 3 cut(s) 430, 550, 632
BfaI CTAG 4 cut(s) 83, 165, 372, 449
BglII AGATCT 1 cut(s) 297
Bme1390I CCNGG 2 cut(s) 336, 597
Bme18I GGWCC 3 cut(s) 332, 341, 437
BmgT120I GGNCC 3 cut(s) 332, 341, 437
BmiI GGNNCC 2 cut(s) 189, 439
BmrFI CCNGG 2 cut(s) 336, 597
BmsI GCATC 4 cut(s) 442, 548, 565, 616
BmtI GCTAGC 1 cut(s) 375
BpiI GAAGAC 2 cut(s) 61, 608
Bpu10I CCTNAGC 2 cut(s) 201, 495
BpuEI CTTGAG 1 cut(s) 162
BsaJI CCNNGG 2 cut(s) 171, 596
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 280
BseBI CCWGG 2 cut(s) 336, 597
BseDI CCNNGG 2 cut(s) 171, 596
BseGI GGATG 2 cut(s) 327, 427
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 3 cut(s) 129, 215, 627
BseRI GAGGAG 5 cut(s) 197, 200, 245, 248, 416
BsiHKAI GWGCWC 1 cut(s) 382
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 1 cut(s) 280
BsmAI GTCTC 3 cut(s) 430, 550, 632
BsmFI GGGAC 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 4 cut(s) 167, 222, 259, 297
BspACI CCGC 1 cut(s) 537
BspCNI CTCAG 3 cut(s) 128, 214, 628
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 189, 439
BspOI GCTAGC 1 cut(s) 375
BspPI GGATC 2 cut(s) 162, 254
BspQI GCTCTTC 1 cut(s) 462
BssECI CCNNGG 2 cut(s) 171, 596
BssMI GATC 4 cut(s) 167, 222, 259, 297
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 336, 597
Bst4CI ACNGT 4 cut(s) 246, 508, 607, 643
Bst6I CTCTTC 1 cut(s) 462
BstAPI GCANNNNNTGC 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 4 cut(s) 115, 201, 495, 636
BstF5I GGATG 2 cut(s) 327, 427
BstKTI GATC 4 cut(s) 170, 225, 262, 300
BstMAI GTCTC 3 cut(s) 430, 550, 632
BstMBI GATC 4 cut(s) 167, 222, 259, 297
BstMWI GCNNNNNNNGC 3 cut(s) 41, 356, 377
BstNI CCWGG 2 cut(s) 336, 597
BstSCI CCNGG 2 cut(s) 334, 595
BstV2I GAAGAC 2 cut(s) 61, 608
BstX2I RGATCY 2 cut(s) 167, 297
BstYI RGATCY 2 cut(s) 167, 297
BtsCI GGATG 2 cut(s) 327, 427
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 75
Cac8I GCNNGC 1 cut(s) 373
CaiI CAGNNNCTG 1 cut(s) 530
CciI TCATGA 1 cut(s) 480
Cfr13I GGNCC 3 cut(s) 332, 341, 437
CseI GACGC 1 cut(s) 377
Csp6I GTAC 3 cut(s) 247, 472, 504
CviAII CATG 3 cut(s) 41, 481, 581
CviQI GTAC 3 cut(s) 247, 472, 504
DdeI CTNAG 4 cut(s) 115, 201, 495, 636
DpnI GATC 4 cut(s) 169, 224, 261, 299
DpnII GATC 4 cut(s) 167, 222, 259, 297
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 382
Eco47I GGWCC 3 cut(s) 332, 341, 437
Eco53kI GAGCTC 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 334, 595
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 3 cut(s) 44, 484, 584
FaiI YATR 9 cut(s) 16, 42, 281, 303, 346, 348, 482, 492, 582
FaqI GGGAC 1 cut(s) 423
FatI CATG 3 cut(s) 40, 480, 580
FauNDI CATATG 1 cut(s) 346
FokI GGATG 2 cut(s) 334, 434
FriOI GRGCYC 1 cut(s) 382
FspBI CTAG 4 cut(s) 83, 165, 372, 449
HgaI GACGC 1 cut(s) 377
Hin1II CATG 3 cut(s) 44, 484, 584
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 1 cut(s) 196
HpaI GTTAAC 1 cut(s) 148
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 5 cut(s) 159, 259, 297, 526, 550
Hpy188III TCNNGA 3 cut(s) 179, 461, 481
Hpy8I GTNNAC 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 103, 284, 646
HpyCH4III ACNGT 4 cut(s) 246, 508, 607, 643
HpyCH4V TGCA 2 cut(s) 359, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 356, 377
HpyF3I CTNAG 4 cut(s) 115, 201, 495, 636
Hsp92II CATG 3 cut(s) 44, 484, 584
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 4 cut(s) 167, 222, 259, 297
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 3 cut(s) 187, 235, 634
LweI GCATC 4 cut(s) 442, 548, 565, 616
MaeI CTAG 4 cut(s) 83, 165, 372, 449
MaeIII GTNAC 2 cut(s) 307, 583
MalI GATC 4 cut(s) 169, 224, 261, 299
MboI GATC 4 cut(s) 167, 222, 259, 297
MboII GAAGA 5 cut(s) 61, 64, 449, 613, 661
MflI RGATCY 2 cut(s) 167, 297
MhlI GDGCHC 1 cut(s) 382
MseI TTAA 2 cut(s) 147, 240
MspR9I CCNGG 2 cut(s) 336, 597
MvaI CCWGG 2 cut(s) 336, 597
MwoI GCNNNNNNNGC 3 cut(s) 41, 356, 377
NdeI CATATG 1 cut(s) 346
NdeII GATC 4 cut(s) 167, 222, 259, 297
NheI GCTAGC 1 cut(s) 371
NlaIII CATG 3 cut(s) 44, 484, 584
NlaIV GGNNCC 2 cut(s) 189, 439
NmeAIII GCCGAG 1 cut(s) 401
PagI TCATGA 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 462
PfeI GAWTC 1 cut(s) 196
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 2 cut(s) 334, 595
PspGI CCWGG 2 cut(s) 334, 595
PspN4I GGNNCC 2 cut(s) 189, 439
PspPI GGNCC 3 cut(s) 332, 341, 437
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 2 cut(s) 167, 297
RsaI GTAC 3 cut(s) 248, 473, 505
RsaNI GTAC 3 cut(s) 247, 472, 504
SacI GAGCTC 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 147, 240
Sau3AI GATC 4 cut(s) 167, 222, 259, 297
Sau96I GGNCC 3 cut(s) 332, 341, 437
ScrFI CCNGG 2 cut(s) 336, 597
SduI GDGCHC 1 cut(s) 382
SetI ASST 8 cut(s) 55, 88, 166, 240, 382, 450, 501, 532
SfaNI GCATC 4 cut(s) 442, 548, 565, 616
SinI GGWCC 3 cut(s) 332, 341, 437
SmlI CTYRAG 1 cut(s) 177
SmoI CTYRAG 1 cut(s) 177
SsiI CCGC 1 cut(s) 537
SspMI CTAG 4 cut(s) 83, 165, 372, 449
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 2 cut(s) 334, 595
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 4 cut(s) 246, 508, 607, 643
TaqI TCGA 1 cut(s) 138
TatI WGTACW 1 cut(s) 471
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 2 cut(s) 147, 240
Tru9I TTAA 2 cut(s) 147, 240
TscAI CASTG 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 469
TspGWI ACGGA 2 cut(s) 242, 345
TspRI CASTG 1 cut(s) 82
VpaK11BI GGWCC 3 cut(s) 332, 341, 437
XspI CTAG 4 cut(s) 83, 165, 372, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.