Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010724

Physical Location & Seq

Genomic Coordinates Reverse (-)

33533785 .. 33535908

Gene Structure 2124 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1267300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGGCTCTGCAACTGTGGGAAACACTGAAAGAGGCGATCGTGGCCTACACCGGCCTCTCTCCGACGACCTTCTTCACTGTGCTAGCTCTCCTCGTGGCCGTCTACCACGTGGCGTCGGGGCTGTTCGGGTCGTCCCAGGACCACCGTCAGCTGGACAGGGACATGGAGGAGATGCAGCCTCTCCCGCCTCCTGTCCAGCTTGGCGAGATCACCGAAGACGAATTGACGCAGTACGATGGCACCGATCCCAAGAAGCCTTTGCTCATGGCTGTCAAGGGTCAGATCTATGACGTGTCACAGGGCAGGATGTTTTATGGACCTGGTGGGCCGTATGCTCTGTTCGCAGGCAAAGATGCTAGCCGAGCTCTTGCTAAAATGTCTTTTGAAGAGAAAGATCTGAATGGTGATATCTCGGGTCTTGGTCCATTTGAACTCGAGGCCTTGCAGGATTGGGAATACAAGTTCATGAGCAAGTATGTCAAGGTTGGATCTATTAAGAGCACAGCTCCGGGAACTGAAGGAGAATCCACTAGTGAAACCGCTATAGCCGCTGATCAAGAAGCTACTAAGCCTGCTGAAGATATTCCATCCGATAGTCCAGCTGCTAAATCCGAGGAAACGTCATCCAGTGCTGAAGCTAAGCAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005794 GO:0005886 GO:0008150 GO:0008289 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0012505 GO:0016020 GO:0019904 GO:0020037 GO:0030308 GO:0031976 GO:0031984 GO:0034357 GO:0040008 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045926 GO:0046906 GO:0048037 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0055035 GO:0065007 GO:0071944 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5

Protein Analysis

217

Amino Acids

23.41

Weight (kDa)

4.45

Isoelectric Point (pI)

54.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	71 - 163	1.5e-11	Cytochrome b5-like Heme/Steroid binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52240 AT5G52240
fragaria_vesca	FvH4_7g29310
malus_domestica	MD01G1199500.v1.1 MD06G1084900.v1.1 MD07G1267300.v1.1 MD13G1074400.v1.1 MD17G1239500.v1.1
prunus_persica	Prupe.2G293000_v2.0.a1
pyrus_communis	pycom01g20940
rosa_chinensis	RchiOBHm_Chr1g0377051
rosa_laevigata	RLG00000026547
rosa_multiflora	Rmu_ssc0000155.1_g000020
rosa_roxburghii	Rroxscaffold_4G00281470
rosa_rugosa	Rorug01G0401300
rosa_samantha	Rh1AG418400 Rh1BG377500 Rh1CG391500 Rh1DG408200
rosa_wichuraiana	Rw1G036710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	239
AccI	GTMKAC	1 cut(s)	102
AciI	CCGC	3 cut(s)	185, 540, 549
AclWI	GGATC	2 cut(s)	239, 496
AcoI	YGGCCR	1 cut(s)	96
AcuI	CTGAAG	2 cut(s)	537, 597
AcvI	CACGTG	1 cut(s)	109
AcyI	GRCGYC	1 cut(s)	113
AfaI	GTAC	1 cut(s)	233
AfiI	CCNNNNNNNGG	1 cut(s)	151
AflIII	ACRYGT	1 cut(s)	291
AgsI	TTSAA	2 cut(s)	386, 431
AhlI	ACTAGT	1 cut(s)	530
AjiI	CACGTC	1 cut(s)	292
AjnI	CCWGG	2 cut(s)	135, 319
AloI	GAACNNNNNNTCC	2 cut(s)	446, 478
AluBI	AGCT	8 cut(s)	86, 151, 199, 365, 506, 563, 602, 638
AluI	AGCT	8 cut(s)	86, 151, 199, 365, 506, 563, 602, 638
Alw21I	GWGCWC	2 cut(s)	367, 503
AlwI	GGATC	2 cut(s)	239, 496
Ama87I	CYCGRG	2 cut(s)	412, 434
AoxI	GGCC	5 cut(s)	42, 52, 96, 326, 438
ApeKI	GCWGC	2 cut(s)	175, 602
Asp700I	GAANNNNTTC	1 cut(s)	582
AspS9I	GGNCC	4 cut(s)	139, 317, 326, 422
AsuC2I	CCSGG	1 cut(s)	510
AsuHPI	GGTGA	2 cut(s)	202, 416
AsuNHI	GCTAGC	2 cut(s)	82, 356
AvaI	CYCGRG	2 cut(s)	412, 434
AvaII	GGWCC	3 cut(s)	139, 317, 422
BanI	GGYRCC	1 cut(s)	239
BanII	GRGCYC	1 cut(s)	367
BauI	CACGAG	1 cut(s)	92
BbrPI	CACGTG	1 cut(s)	109
BbsI	GAAGAC	1 cut(s)	222
Bbv12I	GWGCWC	2 cut(s)	367, 503
BbvI	GCAGC	2 cut(s)	187, 589
BccI	CCATC	2 cut(s)	230, 595
BceAI	ACGGC	2 cut(s)	83, 313
BciT130I	CCWGG	2 cut(s)	137, 321
BclI	TGATCA	1 cut(s)	553
BcnI	CCSGG	1 cut(s)	510
BcuI	ACTAGT	1 cut(s)	530
BfaI	CTAG	3 cut(s)	83, 357, 531
BfmI	CTRYAG	1 cut(s)	543
BglII	AGATCT	2 cut(s)	282, 394
BisI	GCNGC	3 cut(s)	176, 549, 603
BlpI	GCTNAGC	1 cut(s)	639
BlsI	GCNGC	3 cut(s)	177, 550, 604
Bme1390I	CCNGG	3 cut(s)	137, 321, 510
Bme18I	GGWCC	3 cut(s)	139, 317, 422
BmeT110I	CYCGRG	2 cut(s)	412, 434
BmgBI	CACGTC	1 cut(s)	292
BmgT120I	GGNCC	4 cut(s)	139, 317, 326, 422
BmiI	GGNNCC	1 cut(s)	241
BmrFI	CCNGG	3 cut(s)	137, 321, 510
BmsI	GCATC	2 cut(s)	162, 343
BmtI	GCTAGC	2 cut(s)	86, 360
BoxI	GACNNNNGTC	1 cut(s)	144
BpiI	GAAGAC	1 cut(s)	222
BplI	GAGNNNNNCTC	2 cut(s)	490, 522
Bpu1102I	GCTNAGC	1 cut(s)	639
BpuMI	CCSGG	1 cut(s)	510
BsaAI	YACGTR	1 cut(s)	109
BsaHI	GRCGYC	1 cut(s)	113
BsaJI	CCNNGG	2 cut(s)	135, 612
Bsc4I	CCNNNNNNNGG	1 cut(s)	151
Bse118I	RCCGGY	1 cut(s)	50
Bse1I	ACTGG	1 cut(s)	627
BseBI	CCWGG	2 cut(s)	137, 321
BseDI	CCNNGG	2 cut(s)	135, 612
BseGI	GGATG	3 cut(s)	312, 587, 623
BseLI	CCNNNNNNNGG	1 cut(s)	151
BseNI	ACTGG	1 cut(s)	627
BseRI	GAGGAG	2 cut(s)	80, 182
BseXI	GCAGC	2 cut(s)	187, 589
Bsh1285I	CGRYCG	1 cut(s)	39
BshFI	GGCC	5 cut(s)	44, 54, 98, 328, 440
BshNI	GGYRCC	1 cut(s)	239
BsiEI	CGRYCG	1 cut(s)	39
BsiHKAI	GWGCWC	2 cut(s)	367, 503
BsiHKCI	CYCGRG	2 cut(s)	412, 434
BsiSI	CCGG	2 cut(s)	51, 509
BslFI	GGGAC	2 cut(s)	118, 173
BslI	CCNNNNNNNGG	1 cut(s)	151
BsmFI	GGGAC	2 cut(s)	118, 173
BsnI	GGCC	5 cut(s)	44, 54, 98, 328, 440
BsoBI	CYCGRG	2 cut(s)	412, 434
Bsp1286I	GDGCHC	2 cut(s)	367, 503
Bsp143I	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
Bsp1720I	GCTNAGC	1 cut(s)	639
BspACI	CCGC	3 cut(s)	185, 540, 549
BspANI	GGCC	5 cut(s)	44, 54, 98, 328, 440
BspHI	TCATGA	1 cut(s)	465
BspLI	GGNNCC	1 cut(s)	241
BspOI	GCTAGC	2 cut(s)	86, 360
BspPI	GGATC	2 cut(s)	239, 496
BspT107I	GGYRCC	1 cut(s)	239
BsrFI	RCCGGY	1 cut(s)	50
BsrI	ACTGG	1 cut(s)	627
BssAI	RCCGGY	1 cut(s)	50
BssECI	CCNNGG	2 cut(s)	135, 612
BssMI	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
BssNI	GRCGYC	1 cut(s)	113
BssSI	CACGAG	1 cut(s)	92
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	2 cut(s)	137, 321
Bst4CI	ACNGT	3 cut(s)	15, 79, 146
Bst6I	CTCTTC	1 cut(s)	381
BstACI	GRCGYC	1 cut(s)	113
BstBAI	YACGTR	1 cut(s)	109
BstC8I	GCNNGC	4 cut(s)	84, 346, 358, 573
BstDEI	CTNAG	2 cut(s)	567, 639
BstF5I	GGATG	3 cut(s)	312, 587, 623
BstKTI	GATC	7 cut(s)	39, 210, 247, 285, 397, 491, 556
BstMBI	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
BstMCI	CGRYCG	1 cut(s)	39
BstMWI	GCNNNNNNNGC	5 cut(s)	41, 184, 341, 362, 548
BstNI	CCWGG	2 cut(s)	137, 321
BstPAI	GACNNNNGTC	1 cut(s)	144
BstSCI	CCNGG	3 cut(s)	135, 319, 508
BstSFI	CTRYAG	1 cut(s)	543
BstV1I	GCAGC	2 cut(s)	187, 589
BstV2I	GAAGAC	1 cut(s)	222
BstX2I	RGATCY	3 cut(s)	282, 394, 488
BstYI	RGATCY	3 cut(s)	282, 394, 488
BsuRI	GGCC	5 cut(s)	44, 54, 98, 328, 440
BtrI	CACGTC	1 cut(s)	292
BtsCI	GGATG	3 cut(s)	312, 587, 623
BtsIMutI	CAGTG	3 cut(s)	23, 75, 634
Cac8I	GCNNGC	4 cut(s)	84, 346, 358, 573
CciI	TCATGA	1 cut(s)	465
Cfr10I	RCCGGY	1 cut(s)	50
Cfr13I	GGNCC	4 cut(s)	139, 317, 326, 422
CseI	GACGC	2 cut(s)	102, 235
CsiI	ACCWGGT	1 cut(s)	319
Csp6I	GTAC	1 cut(s)	232
CviAII	CATG	3 cut(s)	163, 265, 466
CviQI	GTAC	1 cut(s)	232
DdeI	CTNAG	2 cut(s)	567, 639
DpnI	GATC	7 cut(s)	38, 209, 246, 284, 396, 490, 555
DpnII	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
EaeI	YGGCCR	1 cut(s)	96
Eam1104I	CTCTTC	1 cut(s)	381
EarI	CTCTTC	1 cut(s)	381
Ecl136II	GAGCTC	1 cut(s)	365
Eco147I	AGGCCT	1 cut(s)	440
Eco24I	GRGCYC	1 cut(s)	367
Eco32I	GATATC	1 cut(s)	409
Eco47I	GGWCC	3 cut(s)	139, 317, 422
Eco53kI	GAGCTC	1 cut(s)	365
Eco57I	CTGAAG	2 cut(s)	537, 597
Eco72I	CACGTG	1 cut(s)	109
Eco88I	CYCGRG	2 cut(s)	412, 434
EcoICRI	GAGCTC	1 cut(s)	365
EcoRII	CCWGG	2 cut(s)	135, 319
EcoRV	GATATC	1 cut(s)	409
EcoT38I	GRGCYC	1 cut(s)	367
FaeI	CATG	3 cut(s)	166, 268, 469
FaiI	YATR	8 cut(s)	164, 266, 288, 315, 333, 467, 477, 545
FaqI	GGGAC	2 cut(s)	118, 173
FatI	CATG	3 cut(s)	162, 264, 465
FauI	CCCGC	1 cut(s)	192
FbaI	TGATCA	1 cut(s)	553
FblI	GTMKAC	1 cut(s)	102
Fnu4HI	GCNGC	3 cut(s)	176, 549, 603
FokI	GGATG	3 cut(s)	319, 574, 610
FriOI	GRGCYC	1 cut(s)	367
Fsp4HI	GCNGC	3 cut(s)	176, 549, 603
FspBI	CTAG	3 cut(s)	83, 357, 531
GluI	GCNGC	3 cut(s)	176, 549, 603
HaeIII	GGCC	5 cut(s)	44, 54, 98, 328, 440
HapII	CCGG	2 cut(s)	51, 509
HgaI	GACGC	2 cut(s)	102, 235
Hin1I	GRCGYC	1 cut(s)	113
Hin1II	CATG	3 cut(s)	166, 268, 469
HinfI	GANTC	1 cut(s)	524
HpaII	CCGG	2 cut(s)	51, 509
HphI	GGTGA	2 cut(s)	202, 416
Hpy166II	GTNNAC	1 cut(s)	103
Hpy188I	TCNGA	5 cut(s)	63, 282, 399, 592, 613
Hpy188III	TCNNGA	2 cut(s)	466, 557
Hpy8I	GTNNAC	1 cut(s)	103
Hpy99I	CGWCG	2 cut(s)	67, 118
HpyAV	CCTTC	2 cut(s)	79, 512
HpyCH4III	ACNGT	3 cut(s)	15, 79, 146
HpyCH4IV	ACGT	3 cut(s)	108, 291, 620
HpyCH4V	TGCA	3 cut(s)	10, 175, 445
HpyF10VI	GCNNNNNNNGC	5 cut(s)	41, 184, 341, 362, 548
HpyF3I	CTNAG	2 cut(s)	567, 639
HpySE526I	ACGT	3 cut(s)	108, 291, 620
Hsp92I	GRCGYC	1 cut(s)	113
Hsp92II	CATG	3 cut(s)	166, 268, 469
Ksp22I	TGATCA	1 cut(s)	553
Kzo9I	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
LmnI	GCTCC	1 cut(s)	511
Lsp1109I	GCAGC	2 cut(s)	187, 589
LweI	GCATC	2 cut(s)	162, 343
MabI	ACCWGGT	1 cut(s)	319
MaeI	CTAG	3 cut(s)	83, 357, 531
MaeII	ACGT	3 cut(s)	108, 291, 620
MaeIII	GTNAC	1 cut(s)	294
MalI	GATC	7 cut(s)	38, 209, 246, 284, 396, 490, 555
MboI	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
MboII	GAAGA	4 cut(s)	64, 227, 398, 590
MflI	RGATCY	3 cut(s)	282, 394, 488
MhlI	GDGCHC	2 cut(s)	367, 503
MluCI	AATT	1 cut(s)	221
MmeI	TCCRAC	2 cut(s)	86, 466
MnlI	CCTC	8 cut(s)	25, 65, 101, 160, 189, 198, 430, 607
MroXI	GAANNNNTTC	1 cut(s)	582
MseI	TTAA	1 cut(s)	495
MspA1I	CMGCKG	3 cut(s)	151, 551, 602
MspI	CCGG	2 cut(s)	51, 509
MspR9I	CCNGG	3 cut(s)	137, 321, 510
MvaI	CCWGG	2 cut(s)	137, 321
MwoI	GCNNNNNNNGC	5 cut(s)	41, 184, 341, 362, 548
NciI	CCSGG	1 cut(s)	510
NdeII	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
NheI	GCTAGC	2 cut(s)	82, 356
NlaIII	CATG	3 cut(s)	166, 268, 469
NlaIV	GGNNCC	1 cut(s)	241
NmeAIII	GCCGAG	1 cut(s)	386
NmuCI	GTSAC	1 cut(s)	294
PaeR7I	CTCGAG	1 cut(s)	434
PagI	TCATGA	1 cut(s)	465
PceI	AGGCCT	1 cut(s)	440
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PdmI	GAANNNNTTC	1 cut(s)	582
PfeI	GAWTC	1 cut(s)	524
PfoI	TCCNGGA	1 cut(s)	508
PkrI	GCNGC	3 cut(s)	177, 550, 604
Ple19I	CGATCG	1 cut(s)	39
PmaCI	CACGTG	1 cut(s)	109
PmlI	CACGTG	1 cut(s)	109
Ppu21I	YACGTR	1 cut(s)	109
PshAI	GACNNNNGTC	1 cut(s)	144
Psp124BI	GAGCTC	1 cut(s)	367
Psp6I	CCWGG	2 cut(s)	135, 319
PspCI	CACGTG	1 cut(s)	109
PspGI	CCWGG	2 cut(s)	135, 319
PspN4I	GGNNCC	1 cut(s)	241
PspPI	GGNCC	4 cut(s)	139, 317, 326, 422
PspXI	VCTCGAGB	1 cut(s)	434
PsrI	GAACNNNNNNTAC	2 cut(s)	323, 355
PsuI	RGATCY	3 cut(s)	282, 394, 488
PvuI	CGATCG	1 cut(s)	39
PvuII	CAGCTG	2 cut(s)	151, 602
RsaI	GTAC	1 cut(s)	233
RsaNI	GTAC	1 cut(s)	232
SacI	GAGCTC	1 cut(s)	367
SaqAI	TTAA	1 cut(s)	495
SatI	GCNGC	3 cut(s)	176, 549, 603
Sau3AI	GATC	7 cut(s)	36, 207, 244, 282, 394, 488, 553
Sau96I	GGNCC	4 cut(s)	139, 317, 326, 422
ScrFI	CCNGG	3 cut(s)	137, 321, 510
SduI	GDGCHC	2 cut(s)	367, 503
SexAI	ACCWGGT	1 cut(s)	319
SfaNI	GCATC	2 cut(s)	162, 343
SfcI	CTRYAG	1 cut(s)	543
Sfr274I	CTCGAG	1 cut(s)	434
SinI	GGWCC	3 cut(s)	139, 317, 422
SlaI	CTCGAG	1 cut(s)	434
SmlI	CTYRAG	1 cut(s)	434
SmoI	CTYRAG	1 cut(s)	434
SpeI	ACTAGT	1 cut(s)	530
Sse9I	AATT	1 cut(s)	221
SseBI	AGGCCT	1 cut(s)	440
SsiI	CCGC	3 cut(s)	185, 540, 549
SspMI	CTAG	3 cut(s)	83, 357, 531
SstI	GAGCTC	1 cut(s)	367
StuI	AGGCCT	1 cut(s)	440
StyD4I	CCNGG	3 cut(s)	135, 319, 508
TaaI	ACNGT	3 cut(s)	15, 79, 146
TaiI	ACGT	3 cut(s)	111, 294, 623
TaqI	TCGA	1 cut(s)	435
TasI	AATT	1 cut(s)	221
TauI	GCSGC	1 cut(s)	551
TfiI	GAWTC	1 cut(s)	524
Tru1I	TTAA	1 cut(s)	495
Tru9I	TTAA	1 cut(s)	495
TscAI	CASTG	3 cut(s)	30, 82, 634
TseFI	GTSAC	1 cut(s)	294
TseI	GCWGC	2 cut(s)	175, 602
Tsp45I	GTSAC	1 cut(s)	294
TspDTI	ATGAA	1 cut(s)	454
TspRI	CASTG	3 cut(s)	30, 82, 634
VpaK11BI	GGWCC	3 cut(s)	139, 317, 422
XhoI	CTCGAG	1 cut(s)	434
XmiI	GTMKAC	1 cut(s)	102
XmnI	GAANNNNTTC	1 cut(s)	582
XspI	CTAG	3 cut(s)	83, 357, 531

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.