Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010724

Physical Location & Seq

Genomic Coordinates Forward (+)

5257359 .. 5257744

Gene Structure 386 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1074400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 333 bp

ATGGCTATCAAGGGTCAGATATATGACATGTCCCAGAGCAGGACGTTTTATGGACCTGGTGGGCCTTACGCACTGTTCGCGGAGAAAGATGCTAGCATAGCTCTTGCAAAAATGTCGTTTGAAGACAAAGATCTGACTGGTGATATCTCTGGTCTTAGTCAATTCGAACTCGAGGCCTTGCAGGACGGGGAATACAAGTTCATGAGTAAGTATAGCAAGGTCGGATCTATTAAGAGCACCGCTCCTGCAACTGAAGGAGAATCCACTAGAAGGTACCGTGGAGACGTCGGCTCCGATCTCCAGCTCGAGAGGAATATCATGATTGATCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0012505 GO:0016020 GO:0019904 GO:0020037 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046906 GO:0048037 GO:0055035 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5

Protein Analysis

111

Amino Acids

12.15

Weight (kDa)

4.78

Isoelectric Point (pI)

59.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	1 - 75	3.4e-06	Cytochrome b5-like Heme/Steroid binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52240 AT5G52240
fragaria_vesca	FvH4_7g29310
malus_domestica	MD01G1199500.v1.1 MD06G1084900.v1.1 MD07G1267300.v1.1 MD13G1074400.v1.1 MD17G1239500.v1.1
prunus_persica	Prupe.2G293000_v2.0.a1
pyrus_communis	pycom01g20940
rosa_chinensis	RchiOBHm_Chr1g0377051
rosa_laevigata	RLG00000026547
rosa_multiflora	Rmu_ssc0000155.1_g000020
rosa_roxburghii	Rroxscaffold_4G00281470
rosa_rugosa	Rorug01G0401300
rosa_samantha	Rh1AG418400 Rh1BG377500 Rh1CG391500 Rh1DG408200
rosa_wichuraiana	Rw1G036710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	288
Acc65I	GGTACC	1 cut(s)	273
AccB1I	GGYRCC	1 cut(s)	273
AccBSI	CCGCTC	1 cut(s)	242
AccII	CGCG	1 cut(s)	80
AciI	CCGC	2 cut(s)	80, 240
AclWI	GGATC	1 cut(s)	232
AcuI	CTGAAG	1 cut(s)	273
AcyI	GRCGYC	1 cut(s)	285
AfaI	GTAC	1 cut(s)	275
AfiI	CCNNNNNNNGG	2 cut(s)	39, 270
AflIII	ACRYGT	1 cut(s)	27
AgsI	TTSAA	1 cut(s)	122
AjnI	CCWGG	1 cut(s)	55
AloI	GAACNNNNNNTCC	2 cut(s)	182, 214
AluBI	AGCT	2 cut(s)	101, 304
AluI	AGCT	2 cut(s)	101, 304
Alw21I	GWGCWC	1 cut(s)	239
Alw26I	GTCTC	1 cut(s)	276
AlwI	GGATC	1 cut(s)	232
Ama87I	CYCGRG	2 cut(s)	170, 305
AoxI	GGCC	2 cut(s)	62, 174
Asp718I	GGTACC	1 cut(s)	273
AspS9I	GGNCC	2 cut(s)	53, 62
AsuHPI	GGTGA	1 cut(s)	152
AsuII	TTCGAA	1 cut(s)	165
AsuNHI	GCTAGC	1 cut(s)	92
AvaI	CYCGRG	2 cut(s)	170, 305
AvaII	GGWCC	1 cut(s)	53
BanI	GGYRCC	1 cut(s)	273
BbsI	GAAGAC	1 cut(s)	129
Bbv12I	GWGCWC	1 cut(s)	239
BcgI	CGANNNNNNTGC	2 cut(s)	96, 130
BciT130I	CCWGG	1 cut(s)	57
BclI	TGATCA	1 cut(s)	325
BcoDI	GTCTC	1 cut(s)	276
BfaI	CTAG	2 cut(s)	93, 267
BglII	AGATCT	1 cut(s)	130
Bme1390I	CCNGG	1 cut(s)	57
Bme18I	GGWCC	1 cut(s)	53
BmeT110I	CYCGRG	2 cut(s)	170, 305
BmgT120I	GGNCC	2 cut(s)	53, 62
BmiI	GGNNCC	2 cut(s)	275, 292
BmrFI	CCNGG	1 cut(s)	57
BmsI	GCATC	1 cut(s)	79
BmtI	GCTAGC	1 cut(s)	96
BpiI	GAAGAC	1 cut(s)	129
BplI	GAGNNNNNCTC	2 cut(s)	226, 258
BpmI	CTGGAG	1 cut(s)	284
Bpu14I	TTCGAA	1 cut(s)	165
BsaHI	GRCGYC	1 cut(s)	285
BsaJI	CCNNGG	1 cut(s)	277
BsaXI	ACNNNNNCTCC	2 cut(s)	275, 305
Bsc4I	CCNNNNNNNGG	2 cut(s)	39, 270
Bse1I	ACTGG	1 cut(s)	142
BseBI	CCWGG	1 cut(s)	57
BseDI	CCNNGG	1 cut(s)	277
BseLI	CCNNNNNNNGG	2 cut(s)	39, 270
BseNI	ACTGG	1 cut(s)	142
Bsh1236I	CGCG	1 cut(s)	80
BshFI	GGCC	2 cut(s)	64, 176
BshNI	GGYRCC	1 cut(s)	273
BsiHKAI	GWGCWC	1 cut(s)	239
BsiHKCI	CYCGRG	2 cut(s)	170, 305
BslFI	GGGAC	1 cut(s)	16
BslI	CCNNNNNNNGG	2 cut(s)	39, 270
BsmAI	GTCTC	1 cut(s)	276
BsmBI	CGTCTC	1 cut(s)	276
BsmFI	GGGAC	1 cut(s)	16
BsnI	GGCC	2 cut(s)	64, 176
BsoBI	CYCGRG	2 cut(s)	170, 305
Bsp119I	TTCGAA	1 cut(s)	165
Bsp1286I	GDGCHC	1 cut(s)	239
Bsp143I	GATC	4 cut(s)	130, 224, 295, 325
BspACI	CCGC	2 cut(s)	80, 240
BspANI	GGCC	2 cut(s)	64, 176
BspFNI	CGCG	1 cut(s)	80
BspHI	TCATGA	2 cut(s)	201, 318
BspLI	GGNNCC	2 cut(s)	275, 292
BspOI	GCTAGC	1 cut(s)	96
BspPI	GGATC	1 cut(s)	232
BspT104I	TTCGAA	1 cut(s)	165
BspT107I	GGYRCC	1 cut(s)	273
BsrBI	CCGCTC	1 cut(s)	242
BsrI	ACTGG	1 cut(s)	142
BssECI	CCNNGG	1 cut(s)	277
BssMI	GATC	4 cut(s)	130, 224, 295, 325
BssNI	GRCGYC	1 cut(s)	285
Bst2UI	CCWGG	1 cut(s)	57
Bst4CI	ACNGT	2 cut(s)	75, 278
BstACI	GRCGYC	1 cut(s)	285
BstBI	TTCGAA	1 cut(s)	165
BstC8I	GCNNGC	1 cut(s)	94
BstDEI	CTNAG	1 cut(s)	155
BstDSI	CCRYGG	1 cut(s)	277
BstFNI	CGCG	1 cut(s)	80
BstKTI	GATC	4 cut(s)	133, 227, 298, 328
BstMAI	GTCTC	1 cut(s)	276
BstMBI	GATC	4 cut(s)	130, 224, 295, 325
BstMWI	GCNNNNNNNGC	2 cut(s)	77, 98
BstNI	CCWGG	1 cut(s)	57
BstNSI	RCATGY	1 cut(s)	31
BstSCI	CCNGG	1 cut(s)	55
BstUI	CGCG	1 cut(s)	80
BstV2I	GAAGAC	1 cut(s)	129
BstX2I	RGATCY	2 cut(s)	130, 224
BstYI	RGATCY	2 cut(s)	130, 224
BsuRI	GGCC	2 cut(s)	64, 176
BtgI	CCRYGG	1 cut(s)	277
BtsIMutI	CAGTG	1 cut(s)	71
Cac8I	GCNNGC	1 cut(s)	94
CciI	TCATGA	2 cut(s)	201, 318
Cfr13I	GGNCC	2 cut(s)	53, 62
CsiI	ACCWGGT	1 cut(s)	55
Csp6I	GTAC	1 cut(s)	274
CviAII	CATG	3 cut(s)	28, 202, 319
CviJI	RGCY	6 cut(s)	5, 64, 101, 176, 291, 304
CviKI_1	RGCY	6 cut(s)	5, 64, 101, 176, 291, 304
CviQI	GTAC	1 cut(s)	274
DdeI	CTNAG	1 cut(s)	155
DpnI	GATC	4 cut(s)	132, 226, 297, 327
DpnII	GATC	4 cut(s)	130, 224, 295, 325
Eco147I	AGGCCT	1 cut(s)	176
Eco32I	GATATC	1 cut(s)	145
Eco47I	GGWCC	1 cut(s)	53
Eco57I	CTGAAG	1 cut(s)	273
Eco88I	CYCGRG	2 cut(s)	170, 305
EcoRII	CCWGG	1 cut(s)	55
EcoRV	GATATC	1 cut(s)	145
Esp3I	CGTCTC	1 cut(s)	276
FaeI	CATG	3 cut(s)	31, 205, 322
FaiI	YATR	8 cut(s)	22, 24, 29, 51, 98, 203, 213, 320
FaqI	GGGAC	1 cut(s)	16
FatI	CATG	3 cut(s)	27, 201, 318
FbaI	TGATCA	1 cut(s)	325
FspBI	CTAG	2 cut(s)	93, 267
GsuI	CTGGAG	1 cut(s)	284
HaeIII	GGCC	2 cut(s)	64, 176
Hin1I	GRCGYC	1 cut(s)	285
Hin1II	CATG	3 cut(s)	31, 205, 322
HinfI	GANTC	1 cut(s)	260
HphI	GGTGA	1 cut(s)	152
Hpy188I	TCNGA	4 cut(s)	18, 135, 224, 295
Hpy188III	TCNNGA	3 cut(s)	202, 307, 319
Hpy99I	CGWCG	1 cut(s)	290
HpyAV	CCTTC	2 cut(s)	248, 264
HpyCH4III	ACNGT	2 cut(s)	75, 278
HpyCH4IV	ACGT	2 cut(s)	44, 285
HpyCH4V	TGCA	3 cut(s)	107, 181, 248
HpyF10VI	GCNNNNNNNGC	2 cut(s)	77, 98
HpyF3I	CTNAG	1 cut(s)	155
HpySE526I	ACGT	2 cut(s)	44, 285
Hsp92I	GRCGYC	1 cut(s)	285
Hsp92II	CATG	3 cut(s)	31, 205, 322
KpnI	GGTACC	1 cut(s)	277
Ksp22I	TGATCA	1 cut(s)	325
Kzo9I	GATC	4 cut(s)	130, 224, 295, 325
LmnI	GCTCC	2 cut(s)	247, 296
LpnPI	CCDG	9 cut(s)	25, 42, 47, 69, 123, 135, 167, 258, 314
LweI	GCATC	1 cut(s)	79
MabI	ACCWGGT	1 cut(s)	55
MaeI	CTAG	2 cut(s)	93, 267
MaeII	ACGT	2 cut(s)	44, 285
MalI	GATC	4 cut(s)	132, 226, 297, 327
MbiI	CCGCTC	1 cut(s)	242
MboI	GATC	4 cut(s)	130, 224, 295, 325
MboII	GAAGA	1 cut(s)	134
MflI	RGATCY	2 cut(s)	130, 224
MhlI	GDGCHC	1 cut(s)	239
MluCI	AATT	1 cut(s)	161
MmeI	TCCRAC	1 cut(s)	202
MnlI	CCTC	2 cut(s)	166, 303
MseI	TTAA	1 cut(s)	231
MspR9I	CCNGG	1 cut(s)	57
MvaI	CCWGG	1 cut(s)	57
MvnI	CGCG	1 cut(s)	80
MwoI	GCNNNNNNNGC	2 cut(s)	77, 98
NdeII	GATC	4 cut(s)	130, 224, 295, 325
NheI	GCTAGC	1 cut(s)	92
NlaIII	CATG	3 cut(s)	31, 205, 322
NlaIV	GGNNCC	2 cut(s)	275, 292
NspI	RCATGY	1 cut(s)	31
NspV	TTCGAA	1 cut(s)	165
PaeR7I	CTCGAG	2 cut(s)	170, 305
PagI	TCATGA	2 cut(s)	201, 318
PceI	AGGCCT	1 cut(s)	176
PciI	ACATGT	1 cut(s)	27
PcsI	WCGNNNNNNNCGW	1 cut(s)	291
PfeI	GAWTC	1 cut(s)	260
PscI	ACATGT	1 cut(s)	27
Psp6I	CCWGG	1 cut(s)	55
PspGI	CCWGG	1 cut(s)	55
PspN4I	GGNNCC	2 cut(s)	275, 292
PspPI	GGNCC	2 cut(s)	53, 62
PspXI	VCTCGAGB	1 cut(s)	170
PsuI	RGATCY	2 cut(s)	130, 224
RsaI	GTAC	1 cut(s)	275
RsaNI	GTAC	1 cut(s)	274
SaqAI	TTAA	1 cut(s)	231
Sau3AI	GATC	4 cut(s)	130, 224, 295, 325
Sau96I	GGNCC	2 cut(s)	53, 62
ScrFI	CCNGG	1 cut(s)	57
SduI	GDGCHC	1 cut(s)	239
SetI	ASST	7 cut(s)	47, 58, 103, 222, 275, 288, 306
SexAI	ACCWGGT	1 cut(s)	55
SfaNI	GCATC	1 cut(s)	79
Sfr274I	CTCGAG	2 cut(s)	170, 305
SfuI	TTCGAA	1 cut(s)	165
SinI	GGWCC	1 cut(s)	53
SlaI	CTCGAG	2 cut(s)	170, 305
SmlI	CTYRAG	2 cut(s)	170, 305
SmoI	CTYRAG	2 cut(s)	170, 305
Sse9I	AATT	1 cut(s)	161
SseBI	AGGCCT	1 cut(s)	176
SsiI	CCGC	2 cut(s)	80, 240
SspMI	CTAG	2 cut(s)	93, 267
StuI	AGGCCT	1 cut(s)	176
StyD4I	CCNGG	1 cut(s)	55
TaaI	ACNGT	2 cut(s)	75, 278
TaiI	ACGT	2 cut(s)	47, 288
TaqI	TCGA	3 cut(s)	165, 171, 306
TasI	AATT	1 cut(s)	161
TfiI	GAWTC	1 cut(s)	260
Tru1I	TTAA	1 cut(s)	231
Tru9I	TTAA	1 cut(s)	231
TscAI	CASTG	1 cut(s)	78
TspDTI	ATGAA	1 cut(s)	190
TspRI	CASTG	1 cut(s)	78
VpaK11BI	GGWCC	1 cut(s)	53
XceI	RCATGY	1 cut(s)	31
XhoI	CTCGAG	2 cut(s)	170, 305
XspI	CTAG	2 cut(s)	93, 267
ZraI	GACGTC	1 cut(s)	286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.