Rh1BG377500

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0010724
Physical Location & Seq
Reverse (-)
50722836 .. 50724492
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG377500.1

Sequence Viewer

Length: 627 bp
ATGGCTCTGCAGCTCTGGGAAACGCTGAAGGAGGCGATCGTGGTCTACACGGGCCTATCTCCGGCGACCTTCTTCACACTCCTGGCTCTGCTCTTCGCCGCTTACCATGTCCTTTCTGGACTTTTTGGGCCGTCCGATACCCACCACCGCCCCAGGAGCTTGGAGGAGATGCAGCCTCTTCCGCCGCCGGTCCAGCTCGGCGAGATCACCGCCGAGGAGCTCAAGCAGTACGACGGCTCCGATCCCAAGAAGCCTCTGCTCATGGCCATCAAGTCTCAGATCTATGACGTGTCTCAGAGCAGGATGTTTTATGGACCTGGAGGTCCTTATGCTCTATTTGCAGGAAAAGATGCTAGCAGGGCTCTTGCAAAAATGTCTTTCGAAGACAAAGATCTCACTGGTGATACTACTGGTCTTGGTCCTTTTGAGCTTGAGGCCTTGCAAGATTGGGAATATAAGTTCATGAGCAAGTATGTCAAGGTCGGAACCATTAAGAGCTCAGTTCCAGAAACTCAGGAGGAATCCACTAGTGAGGCCACCAAAGCTGCTGAAGATGTACCATCAGAAAGCCCAGCTGTCAAATCTGAGGAGACCTCCTCGTCTACTGCTGATGCCAAGCAAGAGTAG

Protein Analysis

208

Amino Acids

22.78

Weight (kDa)

4.74

Isoelectric Point (pI)

63.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 70 - 164 1.1e-11 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010724)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52240 AT5G52240
fragaria_vesca FvH4_7g29310
malus_domestica MD01G1199500.v1.1 MD06G1084900.v1.1 MD07G1267300.v1.1 MD13G1074400.v1.1 MD17G1239500.v1.1
prunus_persica Prupe.2G293000_v2.0.a1
pyrus_communis pycom01g20940
rosa_chinensis RchiOBHm_Chr1g0377051
rosa_laevigata RLG00000026547
rosa_multiflora Rmu_ssc0000155.1_g000020
rosa_roxburghii Rroxscaffold_4G00281470
rosa_rugosa Rorug01G0401300
rosa_samantha Rh1AG418400 Rh1BG377500 Rh1CG391500 Rh1DG408200
rosa_wichuraiana Rw1G036710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 321, 598
AccI GTMKAC 2 cut(s) 45, 602
AciI CCGC 5 cut(s) 99, 148, 182, 185, 210
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 264
AcuI CTGAAG 2 cut(s) 47, 570
AfaI GTAC 2 cut(s) 230, 558
AfiI CCNNNNNNNGG 1 cut(s) 61
AflIII ACRYGT 1 cut(s) 288
AhlI ACTAGT 1 cut(s) 527
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 3 cut(s) 81, 152, 316
AloI GAACNNNNNNTCC 2 cut(s) 443, 475
AluBI AGCT 8 cut(s) 13, 159, 196, 220, 430, 498, 545, 575
AluI AGCT 8 cut(s) 13, 159, 196, 220, 430, 498, 545, 575
Alw21I GWGCWC 2 cut(s) 222, 500
Alw26I GTCTC 3 cut(s) 279, 297, 584
AlwI GGATC 1 cut(s) 236
AoxI GGCC 5 cut(s) 52, 128, 264, 435, 534
ApeKI GCWGC 3 cut(s) 10, 172, 545
AspS9I GGNCC 6 cut(s) 52, 128, 190, 314, 323, 419
AsuHPI GGTGA 2 cut(s) 199, 413
AsuII TTCGAA 1 cut(s) 381
AsuNHI GCTAGC 1 cut(s) 353
AvaII GGWCC 4 cut(s) 190, 314, 323, 419
BalI TGGCCA 1 cut(s) 266
BanII GRGCYC 3 cut(s) 222, 364, 500
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 222, 500
BbvI GCAGC 3 cut(s) 22, 184, 532
BccI CCATC 2 cut(s) 275, 568
BceAI ACGGC 2 cut(s) 115, 250
BciT130I CCWGG 3 cut(s) 83, 154, 318
BcoDI GTCTC 3 cut(s) 279, 297, 584
BcuI ACTAGT 1 cut(s) 527
BfaI CTAG 2 cut(s) 354, 528
BfmI CTRYAG 1 cut(s) 8
BglII AGATCT 2 cut(s) 279, 391
BisI GCNGC 5 cut(s) 11, 99, 173, 185, 546
BlsI GCNGC 5 cut(s) 12, 100, 174, 186, 547
Bme1390I CCNGG 3 cut(s) 83, 154, 318
Bme18I GGWCC 4 cut(s) 190, 314, 323, 419
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 6 cut(s) 52, 128, 190, 314, 323, 419
BmiI GGNNCC 2 cut(s) 238, 487
BmrFI CCNGG 3 cut(s) 83, 154, 318
BmsI GCATC 3 cut(s) 159, 340, 601
BmtI GCTAGC 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 390
BplI GAGNNNNNCTC 4 cut(s) 578, 610, 581, 613
BpmI CTGGAG 1 cut(s) 339
Bpu14I TTCGAA 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 206, 452
BsaI GGTCTC 1 cut(s) 584
BsaJI CCNNGG 2 cut(s) 152, 213
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 2 cut(s) 403, 415
BseBI CCWGG 3 cut(s) 83, 154, 318
BseDI CCNNGG 2 cut(s) 152, 213
BseGI GGATG 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 5 cut(s) 290, 308, 513, 527, 576
BseNI ACTGG 2 cut(s) 403, 415
BseRI GAGGAG 4 cut(s) 179, 230, 586, 602
BseXI GCAGC 3 cut(s) 22, 184, 532
BseYI CCCAGC 1 cut(s) 571
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 54, 130, 266, 437, 536
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 2 cut(s) 222, 500
BsiSI CCGG 2 cut(s) 62, 188
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 3 cut(s) 279, 297, 584
BsnI GGCC 5 cut(s) 54, 130, 266, 437, 536
Bso31I GGTCTC 1 cut(s) 584
Bsp119I TTCGAA 1 cut(s) 381
Bsp1286I GDGCHC 3 cut(s) 222, 364, 500
Bsp143I GATC 5 cut(s) 36, 204, 241, 279, 391
BspACI CCGC 5 cut(s) 99, 148, 182, 185, 210
BspANI GGCC 5 cut(s) 54, 130, 266, 437, 536
BspCNI CTCAG 5 cut(s) 289, 307, 512, 526, 577
BspHI TCATGA 1 cut(s) 462
BspLI GGNNCC 2 cut(s) 238, 487
BspMAI CTGCAG 1 cut(s) 12
BspOI GCTAGC 1 cut(s) 357
BspPI GGATC 1 cut(s) 236
BspQI GCTCTTC 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 381
BspTNI GGTCTC 1 cut(s) 584
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 2 cut(s) 403, 415
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 2 cut(s) 152, 213
BssMI GATC 5 cut(s) 36, 204, 241, 279, 391
Bst2UI CCWGG 3 cut(s) 83, 154, 318
Bst6I CTCTTC 2 cut(s) 98, 183
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 5 cut(s) 276, 294, 499, 513, 585
BstF5I GGATG 1 cut(s) 309
BstKTI GATC 5 cut(s) 39, 207, 244, 282, 394
BstMAI GTCTC 3 cut(s) 279, 297, 584
BstMBI GATC 5 cut(s) 36, 204, 241, 279, 391
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 6 cut(s) 156, 181, 193, 338, 359, 542
BstNI CCWGG 3 cut(s) 83, 154, 318
BstSCI CCNGG 3 cut(s) 81, 152, 316
BstSFI CTRYAG 1 cut(s) 8
BstV1I GCAGC 3 cut(s) 22, 184, 532
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 2 cut(s) 279, 391
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 2 cut(s) 279, 391
BsuRI GGCC 5 cut(s) 54, 130, 266, 437, 536
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 396
Cac8I GCNNGC 1 cut(s) 355
CciI TCATGA 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 6 cut(s) 52, 128, 190, 314, 323, 419
Csp6I GTAC 2 cut(s) 229, 557
CviAII CATG 3 cut(s) 107, 262, 463
CviQI GTAC 2 cut(s) 229, 557
DdeI CTNAG 5 cut(s) 276, 294, 499, 513, 585
DpnI GATC 5 cut(s) 38, 206, 243, 281, 393
DpnII GATC 5 cut(s) 36, 204, 241, 279, 391
DrdI GACNNNNNNGTC 2 cut(s) 321, 598
DseDI GACNNNNNNGTC 2 cut(s) 321, 598
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 2 cut(s) 98, 183
EarI CTCTTC 2 cut(s) 98, 183
EciI GGCGGA 1 cut(s) 171
Ecl136II GAGCTC 2 cut(s) 220, 498
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 3 cut(s) 222, 364, 500
Eco31I GGTCTC 1 cut(s) 584
Eco47I GGWCC 4 cut(s) 190, 314, 323, 419
Eco53kI GAGCTC 2 cut(s) 220, 498
Eco57I CTGAAG 2 cut(s) 47, 570
EcoICRI GAGCTC 2 cut(s) 220, 498
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 3 cut(s) 81, 152, 316
EcoT38I GRGCYC 3 cut(s) 222, 364, 500
FaeI CATG 3 cut(s) 110, 265, 466
FaiI YATR 8 cut(s) 108, 263, 285, 312, 330, 456, 464, 474
FatI CATG 3 cut(s) 106, 261, 462
FblI GTMKAC 2 cut(s) 45, 602
Fnu4HI GCNGC 5 cut(s) 11, 99, 173, 185, 546
FokI GGATG 1 cut(s) 316
FriOI GRGCYC 3 cut(s) 222, 364, 500
Fsp4HI GCNGC 5 cut(s) 11, 99, 173, 185, 546
FspBI CTAG 2 cut(s) 354, 528
GluI GCNGC 5 cut(s) 11, 99, 173, 185, 546
GsaI CCCAGC 1 cut(s) 575
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 5 cut(s) 54, 130, 266, 437, 536
HapII CCGG 2 cut(s) 62, 188
Hin1II CATG 3 cut(s) 110, 265, 466
HinfI GANTC 1 cut(s) 521
HpaII CCGG 2 cut(s) 62, 188
HphI GGTGA 2 cut(s) 199, 413
Hpy166II GTNNAC 2 cut(s) 46, 603
Hpy188I TCNGA 7 cut(s) 136, 241, 279, 297, 485, 565, 586
Hpy188III TCNNGA 4 cut(s) 117, 463, 506, 515
Hpy8I GTNNAC 2 cut(s) 46, 603
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 22, 79
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 5 cut(s) 10, 172, 341, 368, 442
HpyF10VI GCNNNNNNNGC 6 cut(s) 156, 181, 193, 338, 359, 542
HpyF3I CTNAG 5 cut(s) 276, 294, 499, 513, 585
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 3 cut(s) 110, 265, 466
Kzo9I GATC 5 cut(s) 36, 204, 241, 279, 391
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 156, 217, 242
Lsp1109I GCAGC 3 cut(s) 22, 184, 532
LweI GCATC 3 cut(s) 159, 340, 601
MaeI CTAG 2 cut(s) 354, 528
MaeII ACGT 1 cut(s) 288
MalI GATC 5 cut(s) 38, 206, 243, 281, 393
MboI GATC 5 cut(s) 36, 204, 241, 279, 391
MboII GAAGA 5 cut(s) 64, 85, 170, 395, 563
MflI RGATCY 2 cut(s) 279, 391
MhlI GDGCHC 3 cut(s) 222, 364, 500
MlsI TGGCCA 1 cut(s) 266
MluNI TGGCCA 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 463
Mox20I TGGCCA 1 cut(s) 266
MscI TGGCCA 1 cut(s) 266
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 2 cut(s) 62, 188
MspR9I CCNGG 3 cut(s) 83, 154, 318
MvaI CCWGG 3 cut(s) 83, 154, 318
MwoI GCNNNNNNNGC 6 cut(s) 156, 181, 193, 338, 359, 542
NdeII GATC 5 cut(s) 36, 204, 241, 279, 391
NheI GCTAGC 1 cut(s) 353
NlaIII CATG 3 cut(s) 110, 265, 466
NlaIV GGNNCC 2 cut(s) 238, 487
NmeAIII GCCGAG 2 cut(s) 177, 238
NspV TTCGAA 1 cut(s) 381
PagI TCATGA 1 cut(s) 462
PceI AGGCCT 1 cut(s) 437
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 521
PkrI GCNGC 5 cut(s) 12, 100, 174, 186, 547
Ple19I CGATCG 1 cut(s) 39
PpuMI RGGWCCY 1 cut(s) 323
Psp124BI GAGCTC 2 cut(s) 222, 500
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 81, 152, 316
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 3 cut(s) 81, 152, 316
PspN4I GGNNCC 2 cut(s) 238, 487
PspPI GGNCC 6 cut(s) 52, 128, 190, 314, 323, 419
PspPPI RGGWCCY 1 cut(s) 323
PstI CTGCAG 1 cut(s) 12
PsuI RGATCY 2 cut(s) 279, 391
PvuI CGATCG 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 230, 558
RsaNI GTAC 2 cut(s) 229, 557
SacI GAGCTC 2 cut(s) 222, 500
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 5 cut(s) 11, 99, 173, 185, 546
Sau3AI GATC 5 cut(s) 36, 204, 241, 279, 391
Sau96I GGNCC 6 cut(s) 52, 128, 190, 314, 323, 419
ScrFI CCNGG 3 cut(s) 83, 154, 318
SduI GDGCHC 3 cut(s) 222, 364, 500
SfaNI GCATC 3 cut(s) 159, 340, 601
SfcI CTRYAG 1 cut(s) 8
SfuI TTCGAA 1 cut(s) 381
SinI GGWCC 4 cut(s) 190, 314, 323, 419
SmlI CTYRAG 2 cut(s) 221, 431
SmoI CTYRAG 2 cut(s) 221, 431
SpeI ACTAGT 1 cut(s) 527
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 5 cut(s) 99, 148, 182, 185, 210
SspMI CTAG 2 cut(s) 354, 528
SstI GAGCTC 2 cut(s) 222, 500
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 3 cut(s) 81, 152, 316
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 381
TauI GCSGC 2 cut(s) 101, 187
TfiI GAWTC 1 cut(s) 521
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 1 cut(s) 403
TseI GCWGC 3 cut(s) 10, 172, 545
TspDTI ATGAA 1 cut(s) 451
TspRI CASTG 1 cut(s) 403
VpaK11BI GGWCC 4 cut(s) 190, 314, 323, 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XmiI GTMKAC 2 cut(s) 45, 602
XspI CTAG 2 cut(s) 354, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.