Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0010724

Physical Location & Seq

Genomic Coordinates Reverse (-)

29670706 .. 29672747

Gene Structure 2042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1199500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGTCTCTGCAACTGTGGGAAACACTGAAAGAGGCGATCGCGGCCTACACAGGCCTCTCTCCGACGACCTTCTTCACTATGCTAGCTGTGCTTGTGGCCATCTATCATGTGGCGTCGGGGTTGTTTGGGTCGTCCGACAACCACCAGCGGGCCAGGAGCTCGGAGGAGGAGATGCAGCCTCTGCCGCCTCCCGTCCAGCTCGGCGAGATCACCGAAGAAGAATTGAAGCAGTACGACGGCTCTGATTCCAAGAAGCCTCTGCTCATGGCTATCAAGGGTCAGATCTATGACGTGTCCCAGAGCAGGATGTTTTATGGACCTGGTGGGCCTTACGCACTGTTCGCGGGGAAAGATGCTAGCAGAGCTCTTGCAAAAATGTCGTTTGAAGACAAAGATCTGACTGGTGATATCTCTGGTCTTGGTCCATTCGAACTCGAGGCCTTGCAGGACTGGGAATACAAGTTCATGAGCAAGTATGTCAAGGTCGGATCTATTAAGAGCACCGCTCCTGCAACTGAAGGAGAATCCACTGGCGAAACTGCTAAAGCCATCGATCAAGATGCTGCTAAGCCTGCTGAAGATATTCCATCCGATAGTCCAGCTGTTAAATCCGAGGAAACCTCATCTAGTGCTGAAGCTAAGCAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0005488 GO:0005496 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005794 GO:0005886 GO:0008150 GO:0008289 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0012505 GO:0016020 GO:0019904 GO:0020037 GO:0030308 GO:0031976 GO:0031984 GO:0034357 GO:0040008 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045926 GO:0046906 GO:0048037 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0055035 GO:0065007 GO:0071944 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cyt-b5

Protein Analysis

217

Amino Acids

23.38

Weight (kDa)

4.6

Isoelectric Point (pI)

65.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cyt-b5	PF00173	71 - 163	9.5e-13	Cytochrome b5-like Heme/Steroid binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52240 AT5G52240
fragaria_vesca	FvH4_7g29310
malus_domestica	MD01G1199500.v1.1 MD06G1084900.v1.1 MD07G1267300.v1.1 MD13G1074400.v1.1 MD17G1239500.v1.1
prunus_persica	Prupe.2G293000_v2.0.a1
pyrus_communis	pycom01g20940
rosa_chinensis	RchiOBHm_Chr1g0377051
rosa_laevigata	RLG00000026547
rosa_multiflora	Rmu_ssc0000155.1_g000020
rosa_roxburghii	Rroxscaffold_4G00281470
rosa_rugosa	Rorug01G0401300
rosa_samantha	Rh1AG418400 Rh1BG377500 Rh1CG391500 Rh1DG408200
rosa_wichuraiana	Rw1G036710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	506
AccII	CGCG	2 cut(s)	41, 344
AciI	CCGC	5 cut(s)	41, 148, 185, 344, 504
AclWI	GGATC	1 cut(s)	496
AcoI	YGGCCR	1 cut(s)	96
AcuI	CTGAAG	2 cut(s)	537, 597
AcyI	GRCGYC	1 cut(s)	113
AfaI	GTAC	1 cut(s)	233
AfiI	CCNNNNNNNGG	2 cut(s)	148, 303
AflIII	ACRYGT	1 cut(s)	291
AgsI	TTSAA	2 cut(s)	226, 386
AjiI	CACGTC	1 cut(s)	292
AjnI	CCWGG	2 cut(s)	152, 319
AloI	GAACNNNNNNTCC	2 cut(s)	446, 478
AluBI	AGCT	6 cut(s)	86, 159, 199, 365, 602, 638
AluI	AGCT	6 cut(s)	86, 159, 199, 365, 602, 638
Alw21I	GWGCWC	3 cut(s)	161, 367, 503
Alw26I	GTCTC	1 cut(s)	9
AlwI	GGATC	1 cut(s)	496
AlwNI	CAGNNNCTG	1 cut(s)	181
Ama87I	CYCGRG	1 cut(s)	434
AoxI	GGCC	6 cut(s)	42, 52, 96, 150, 326, 438
ApeKI	GCWGC	2 cut(s)	175, 563
AsiSI	GCGATCGC	1 cut(s)	39
Asp700I	GAANNNNTTC	1 cut(s)	582
AspS9I	GGNCC	4 cut(s)	150, 317, 326, 422
AsuHPI	GGTGA	2 cut(s)	202, 416
AsuII	TTCGAA	1 cut(s)	429
AsuNHI	GCTAGC	2 cut(s)	82, 356
AvaI	CYCGRG	1 cut(s)	434
AvaII	GGWCC	2 cut(s)	317, 422
BalI	TGGCCA	1 cut(s)	98
BanII	GRGCYC	2 cut(s)	161, 367
BbsI	GAAGAC	1 cut(s)	393
Bbv12I	GWGCWC	3 cut(s)	161, 367, 503
BbvI	GCAGC	2 cut(s)	187, 550
BccI	CCATC	3 cut(s)	107, 557, 595
BceAI	ACGGC	1 cut(s)	253
BcgI	CGANNNNNNTGC	4 cut(s)	360, 394, 542, 576
BciT130I	CCWGG	2 cut(s)	154, 321
BcoDI	GTCTC	1 cut(s)	9
BfaI	CTAG	3 cut(s)	83, 357, 627
BglII	AGATCT	2 cut(s)	282, 394
BisI	GCNGC	4 cut(s)	42, 176, 185, 564
BlpI	GCTNAGC	2 cut(s)	567, 639
BlsI	GCNGC	4 cut(s)	43, 177, 186, 565
Bme1390I	CCNGG	2 cut(s)	154, 321
Bme18I	GGWCC	2 cut(s)	317, 422
BmeT110I	CYCGRG	1 cut(s)	434
BmgBI	CACGTC	1 cut(s)	292
BmgT120I	GGNCC	4 cut(s)	150, 317, 326, 422
BmrFI	CCNGG	2 cut(s)	154, 321
BmrI	ACTGGG	1 cut(s)	460
BmsI	GCATC	3 cut(s)	162, 343, 550
BmtI	GCTAGC	2 cut(s)	86, 360
BmuI	ACTGGG	1 cut(s)	460
BpiI	GAAGAC	1 cut(s)	393
BplI	GAGNNNNNCTC	4 cut(s)	490, 522, 605, 637
Bpu1102I	GCTNAGC	2 cut(s)	567, 639
Bpu14I	TTCGAA	1 cut(s)	429
Bsa29I	ATCGAT	1 cut(s)	552
BsaHI	GRCGYC	1 cut(s)	113
BsaJI	CCNNGG	1 cut(s)	612
Bsc4I	CCNNNNNNNGG	2 cut(s)	148, 303
Bse1I	ACTGG	3 cut(s)	406, 455, 535
BseBI	CCWGG	2 cut(s)	154, 321
BseCI	ATCGAT	1 cut(s)	552
BseDI	CCNNGG	1 cut(s)	612
BseGI	GGATG	2 cut(s)	312, 587
BseLI	CCNNNNNNNGG	2 cut(s)	148, 303
BseNI	ACTGG	3 cut(s)	406, 455, 535
BseRI	GAGGAG	2 cut(s)	179, 182
BseXI	GCAGC	2 cut(s)	187, 550
Bsh1236I	CGCG	2 cut(s)	41, 344
Bsh1285I	CGRYCG	1 cut(s)	39
BshFI	GGCC	6 cut(s)	44, 54, 98, 152, 328, 440
BshVI	ATCGAT	1 cut(s)	552
BsiEI	CGRYCG	1 cut(s)	39
BsiHKAI	GWGCWC	3 cut(s)	161, 367, 503
BsiHKCI	CYCGRG	1 cut(s)	434
BslFI	GGGAC	1 cut(s)	280
BslI	CCNNNNNNNGG	2 cut(s)	148, 303
BsmAI	GTCTC	1 cut(s)	9
BsmFI	GGGAC	1 cut(s)	280
BsnI	GGCC	6 cut(s)	44, 54, 98, 152, 328, 440
BsoBI	CYCGRG	1 cut(s)	434
Bsp119I	TTCGAA	1 cut(s)	429
Bsp1286I	GDGCHC	3 cut(s)	161, 367, 503
Bsp143I	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
Bsp1720I	GCTNAGC	2 cut(s)	567, 639
BspACI	CCGC	5 cut(s)	41, 148, 185, 344, 504
BspANI	GGCC	6 cut(s)	44, 54, 98, 152, 328, 440
BspDI	ATCGAT	1 cut(s)	552
BspFNI	CGCG	2 cut(s)	41, 344
BspHI	TCATGA	1 cut(s)	465
BspOI	GCTAGC	2 cut(s)	86, 360
BspPI	GGATC	1 cut(s)	496
BspT104I	TTCGAA	1 cut(s)	429
BsrBI	CCGCTC	1 cut(s)	506
BsrI	ACTGG	3 cut(s)	406, 455, 535
BssECI	CCNNGG	1 cut(s)	612
BssMI	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
BssNI	GRCGYC	1 cut(s)	113
Bst2UI	CCWGG	2 cut(s)	154, 321
Bst4CI	ACNGT	2 cut(s)	15, 339
BstACI	GRCGYC	1 cut(s)	113
BstAPI	GCANNNNNTGC	1 cut(s)	181
BstBI	TTCGAA	1 cut(s)	429
BstC8I	GCNNGC	4 cut(s)	84, 150, 358, 573
BstDEI	CTNAG	2 cut(s)	567, 639
BstF5I	GGATG	2 cut(s)	312, 587
BstFNI	CGCG	2 cut(s)	41, 344
BstKTI	GATC	6 cut(s)	39, 210, 285, 397, 491, 556
BstMAI	GTCTC	1 cut(s)	9
BstMBI	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
BstMCI	CGRYCG	1 cut(s)	39
BstMWI	GCNNNNNNNGC	7 cut(s)	41, 88, 181, 184, 341, 362, 572
BstNI	CCWGG	2 cut(s)	154, 321
BstSCI	CCNGG	2 cut(s)	152, 319
BstUI	CGCG	2 cut(s)	41, 344
BstV1I	GCAGC	2 cut(s)	187, 550
BstV2I	GAAGAC	1 cut(s)	393
BstX2I	RGATCY	3 cut(s)	282, 394, 488
BstYI	RGATCY	3 cut(s)	282, 394, 488
Bsu15I	ATCGAT	1 cut(s)	552
BsuRI	GGCC	6 cut(s)	44, 54, 98, 152, 328, 440
BsuTUI	ATCGAT	1 cut(s)	552
BtrI	CACGTC	1 cut(s)	292
BtsCI	GGATG	2 cut(s)	312, 587
BtsIMutI	CAGTG	3 cut(s)	23, 335, 528
Cac8I	GCNNGC	4 cut(s)	84, 150, 358, 573
CaiI	CAGNNNCTG	1 cut(s)	181
CciI	TCATGA	1 cut(s)	465
Cfr13I	GGNCC	4 cut(s)	150, 317, 326, 422
ClaI	ATCGAT	1 cut(s)	552
CseI	GACGC	1 cut(s)	102
CsiI	ACCWGGT	1 cut(s)	319
Csp6I	GTAC	1 cut(s)	232
CviAII	CATG	3 cut(s)	107, 265, 466
CviQI	GTAC	1 cut(s)	232
DdeI	CTNAG	2 cut(s)	567, 639
DpnI	GATC	6 cut(s)	38, 209, 284, 396, 490, 555
DpnII	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
EaeI	YGGCCR	1 cut(s)	96
Ecl136II	GAGCTC	2 cut(s)	159, 365
Eco147I	AGGCCT	2 cut(s)	54, 440
Eco24I	GRGCYC	2 cut(s)	161, 367
Eco32I	GATATC	1 cut(s)	409
Eco47I	GGWCC	2 cut(s)	317, 422
Eco53kI	GAGCTC	2 cut(s)	159, 365
Eco57I	CTGAAG	2 cut(s)	537, 597
Eco88I	CYCGRG	1 cut(s)	434
EcoICRI	GAGCTC	2 cut(s)	159, 365
EcoRII	CCWGG	2 cut(s)	152, 319
EcoRV	GATATC	1 cut(s)	409
EcoT38I	GRGCYC	2 cut(s)	161, 367
FaeI	CATG	3 cut(s)	110, 268, 469
FaiI	YATR	7 cut(s)	80, 108, 266, 288, 315, 467, 477
FaqI	GGGAC	1 cut(s)	280
FatI	CATG	3 cut(s)	106, 264, 465
FauI	CCCGC	2 cut(s)	141, 337
Fnu4HI	GCNGC	4 cut(s)	42, 176, 185, 564
FokI	GGATG	2 cut(s)	319, 574
FriOI	GRGCYC	2 cut(s)	161, 367
Fsp4HI	GCNGC	4 cut(s)	42, 176, 185, 564
FspBI	CTAG	3 cut(s)	83, 357, 627
GluI	GCNGC	4 cut(s)	42, 176, 185, 564
HaeIII	GGCC	6 cut(s)	44, 54, 98, 152, 328, 440
HgaI	GACGC	1 cut(s)	102
Hin1I	GRCGYC	1 cut(s)	113
Hin1II	CATG	3 cut(s)	110, 268, 469
HinfI	GANTC	2 cut(s)	245, 524
HphI	GGTGA	2 cut(s)	202, 416
Hpy188I	TCNGA	9 cut(s)	63, 136, 163, 244, 282, 399, 488, 592, 613
Hpy188III	TCNNGA	2 cut(s)	466, 557
Hpy99I	CGWCG	3 cut(s)	67, 118, 239
HpyAV	CCTTC	2 cut(s)	79, 512
HpyCH4III	ACNGT	2 cut(s)	15, 339
HpyCH4IV	ACGT	1 cut(s)	291
HpyCH4V	TGCA	5 cut(s)	10, 175, 371, 445, 512
HpyF10VI	GCNNNNNNNGC	7 cut(s)	41, 88, 181, 184, 341, 362, 572
HpyF3I	CTNAG	2 cut(s)	567, 639
HpySE526I	ACGT	1 cut(s)	291
Hsp92I	GRCGYC	1 cut(s)	113
Hsp92II	CATG	3 cut(s)	110, 268, 469
Kzo9I	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
LmnI	GCTCC	2 cut(s)	156, 511
Lsp1109I	GCAGC	2 cut(s)	187, 550
LweI	GCATC	3 cut(s)	162, 343, 550
MabI	ACCWGGT	1 cut(s)	319
MaeI	CTAG	3 cut(s)	83, 357, 627
MaeII	ACGT	1 cut(s)	291
MalI	GATC	6 cut(s)	38, 209, 284, 396, 490, 555
MbiI	CCGCTC	1 cut(s)	506
MboI	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
MboII	GAAGA	5 cut(s)	64, 227, 230, 398, 590
MflI	RGATCY	3 cut(s)	282, 394, 488
MhlI	GDGCHC	3 cut(s)	161, 367, 503
MlsI	TGGCCA	1 cut(s)	98
MluCI	AATT	1 cut(s)	221
MluNI	TGGCCA	1 cut(s)	98
MmeI	TCCRAC	3 cut(s)	86, 159, 466
Mox20I	TGGCCA	1 cut(s)	98
MroXI	GAANNNNTTC	1 cut(s)	582
MscI	TGGCCA	1 cut(s)	98
MseI	TTAA	2 cut(s)	495, 606
Msp20I	TGGCCA	1 cut(s)	98
MspA1I	CMGCKG	2 cut(s)	148, 602
MspR9I	CCNGG	2 cut(s)	154, 321
MvaI	CCWGG	2 cut(s)	154, 321
MvnI	CGCG	2 cut(s)	41, 344
MwoI	GCNNNNNNNGC	7 cut(s)	41, 88, 181, 184, 341, 362, 572
NdeII	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
NheI	GCTAGC	2 cut(s)	82, 356
NlaIII	CATG	3 cut(s)	110, 268, 469
NmeAIII	GCCGAG	1 cut(s)	180
NspV	TTCGAA	1 cut(s)	429
PaeR7I	CTCGAG	1 cut(s)	434
PagI	TCATGA	1 cut(s)	465
PceI	AGGCCT	2 cut(s)	54, 440
PdmI	GAANNNNTTC	1 cut(s)	582
PfeI	GAWTC	2 cut(s)	245, 524
PkrI	GCNGC	4 cut(s)	43, 177, 186, 565
Ple19I	CGATCG	1 cut(s)	39
Psp124BI	GAGCTC	2 cut(s)	161, 367
Psp6I	CCWGG	2 cut(s)	152, 319
PspGI	CCWGG	2 cut(s)	152, 319
PspPI	GGNCC	4 cut(s)	150, 317, 326, 422
PspXI	VCTCGAGB	1 cut(s)	434
PstNI	CAGNNNCTG	1 cut(s)	181
PsuI	RGATCY	3 cut(s)	282, 394, 488
PvuI	CGATCG	1 cut(s)	39
PvuII	CAGCTG	1 cut(s)	602
RgaI	GCGATCGC	1 cut(s)	39
RsaI	GTAC	1 cut(s)	233
RsaNI	GTAC	1 cut(s)	232
SacI	GAGCTC	2 cut(s)	161, 367
SaqAI	TTAA	2 cut(s)	495, 606
SatI	GCNGC	4 cut(s)	42, 176, 185, 564
Sau3AI	GATC	6 cut(s)	36, 207, 282, 394, 488, 553
Sau96I	GGNCC	4 cut(s)	150, 317, 326, 422
ScrFI	CCNGG	2 cut(s)	154, 321
SduI	GDGCHC	3 cut(s)	161, 367, 503
SexAI	ACCWGGT	1 cut(s)	319
SfaAI	GCGATCGC	1 cut(s)	39
SfaNI	GCATC	3 cut(s)	162, 343, 550
Sfr274I	CTCGAG	1 cut(s)	434
SfuI	TTCGAA	1 cut(s)	429
SgfI	GCGATCGC	1 cut(s)	39
SinI	GGWCC	2 cut(s)	317, 422
SlaI	CTCGAG	1 cut(s)	434
SmlI	CTYRAG	1 cut(s)	434
SmoI	CTYRAG	1 cut(s)	434
Sse9I	AATT	1 cut(s)	221
SseBI	AGGCCT	2 cut(s)	54, 440
SsiI	CCGC	5 cut(s)	41, 148, 185, 344, 504
SspMI	CTAG	3 cut(s)	83, 357, 627
SstI	GAGCTC	2 cut(s)	161, 367
StuI	AGGCCT	2 cut(s)	54, 440
StyD4I	CCNGG	2 cut(s)	152, 319
TaaI	ACNGT	2 cut(s)	15, 339
TaiI	ACGT	1 cut(s)	294
TaqI	TCGA	3 cut(s)	429, 435, 552
TasI	AATT	1 cut(s)	221
TauI	GCSGC	2 cut(s)	44, 187
TfiI	GAWTC	2 cut(s)	245, 524
Tru1I	TTAA	2 cut(s)	495, 606
Tru9I	TTAA	2 cut(s)	495, 606
TscAI	CASTG	3 cut(s)	30, 342, 535
TseI	GCWGC	2 cut(s)	175, 563
TspDTI	ATGAA	1 cut(s)	454
TspRI	CASTG	3 cut(s)	30, 342, 535
VpaK11BI	GGWCC	2 cut(s)	317, 422
XcmI	CCANNNNNNNNNTGG	1 cut(s)	106
XhoI	CTCGAG	1 cut(s)	434
XmnI	GAANNNNTTC	1 cut(s)	582
XspI	CTAG	3 cut(s)	83, 357, 627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.