MD06G1084900.v1.1

steroid binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010724
Physical Location & Seq
Reverse (-)
20817454 .. 20817636
183 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1084900.v1.1.491

Sequence Viewer

Length: 183 bp
ATGGCTATCAAGGGTCAGATCTATGACATGTCCCAGAGCAGGACGTTTTATGGACCTGGTGGGCCTTACGCACTGTTCGCGGGGAAAGATGCTAGCATAACTCTTGCAAAAATGTCGTTTGAAGACAAAGATCTGACTGGTGATATCTCTGGTCTTGGTCCATTCGAACTCGAGGCCTTGTAG

Protein Analysis

61

Amino Acids

6.42

Weight (kDa)

4.46

Isoelectric Point (pI)

61.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 1 - 43 1.3e-07 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010724)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52240 AT5G52240
fragaria_vesca FvH4_7g29310
malus_domestica MD01G1199500.v1.1 MD06G1084900.v1.1 MD07G1267300.v1.1 MD13G1074400.v1.1 MD17G1239500.v1.1
prunus_persica Prupe.2G293000_v2.0.a1
pyrus_communis pycom01g20940
rosa_chinensis RchiOBHm_Chr1g0377051
rosa_laevigata RLG00000026547
rosa_multiflora Rmu_ssc0000155.1_g000020
rosa_roxburghii Rroxscaffold_4G00281470
rosa_rugosa Rorug01G0401300
rosa_samantha Rh1AG418400 Rh1BG377500 Rh1CG391500 Rh1DG408200
rosa_wichuraiana Rw1G036710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 80
AciI CCGC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 39
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 1 cut(s) 55
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 62, 174
AspS9I GGNCC 3 cut(s) 53, 62, 158
AsuHPI GGTGA 1 cut(s) 152
AsuII TTCGAA 1 cut(s) 165
AsuNHI GCTAGC 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 53, 158
BbsI GAAGAC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BciT130I CCWGG 1 cut(s) 57
BfaI CTAG 1 cut(s) 93
BglII AGATCT 2 cut(s) 18, 130
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 2 cut(s) 53, 158
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 3 cut(s) 53, 62, 158
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 1 cut(s) 79
BmtI GCTAGC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 129
Bpu14I TTCGAA 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 39
Bse1I ACTGG 1 cut(s) 142
BseBI CCWGG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 142
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 2 cut(s) 64, 176
BsiHKCI CYCGRG 1 cut(s) 170
BslFI GGGAC 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 16
BsnI GGCC 2 cut(s) 64, 176
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 165
Bsp143I GATC 2 cut(s) 18, 130
BspACI CCGC 1 cut(s) 80
BspANI GGCC 2 cut(s) 64, 176
BspFNI CGCG 1 cut(s) 80
BspOI GCTAGC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 165
BsrI ACTGG 1 cut(s) 142
BssMI GATC 2 cut(s) 18, 130
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 94
BstFNI CGCG 1 cut(s) 80
BstKTI GATC 2 cut(s) 21, 133
BstMBI GATC 2 cut(s) 18, 130
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 55
BstUI CGCG 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 2 cut(s) 18, 130
BstYI RGATCY 2 cut(s) 18, 130
BsuRI GGCC 2 cut(s) 64, 176
BtsIMutI CAGTG 1 cut(s) 71
Cac8I GCNNGC 1 cut(s) 94
Cfr13I GGNCC 3 cut(s) 53, 62, 158
CsiI ACCWGGT 1 cut(s) 55
CviAII CATG 1 cut(s) 28
CviJI RGCY 3 cut(s) 5, 64, 176
CviKI_1 RGCY 3 cut(s) 5, 64, 176
DpnI GATC 2 cut(s) 20, 132
DpnII GATC 2 cut(s) 18, 130
Eco147I AGGCCT 1 cut(s) 176
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 53, 158
Eco88I CYCGRG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 55
EcoRV GATATC 1 cut(s) 145
FaeI CATG 1 cut(s) 31
FaiI YATR 4 cut(s) 24, 29, 51, 98
FaqI GGGAC 1 cut(s) 16
FatI CATG 1 cut(s) 27
FauI CCCGC 1 cut(s) 73
FspBI CTAG 1 cut(s) 93
HaeIII GGCC 2 cut(s) 64, 176
Hin1II CATG 1 cut(s) 31
HphI GGTGA 1 cut(s) 152
Hpy188I TCNGA 2 cut(s) 18, 135
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 1 cut(s) 107
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 1 cut(s) 31
Kzo9I GATC 2 cut(s) 18, 130
LpnPI CCDG 6 cut(s) 25, 42, 47, 69, 123, 135
LweI GCATC 1 cut(s) 79
MabI ACCWGGT 1 cut(s) 55
MaeI CTAG 1 cut(s) 93
MaeII ACGT 1 cut(s) 44
MalI GATC 2 cut(s) 20, 132
MboI GATC 2 cut(s) 18, 130
MboII GAAGA 1 cut(s) 134
MflI RGATCY 2 cut(s) 18, 130
MnlI CCTC 1 cut(s) 166
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeII GATC 2 cut(s) 18, 130
NheI GCTAGC 1 cut(s) 92
NlaIII CATG 1 cut(s) 31
NspI RCATGY 1 cut(s) 31
NspV TTCGAA 1 cut(s) 165
PaeR7I CTCGAG 1 cut(s) 170
PceI AGGCCT 1 cut(s) 176
PciI ACATGT 1 cut(s) 27
PscI ACATGT 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 55
PspGI CCWGG 1 cut(s) 55
PspPI GGNCC 3 cut(s) 53, 62, 158
PspXI VCTCGAGB 1 cut(s) 170
PsuI RGATCY 2 cut(s) 18, 130
Sau3AI GATC 2 cut(s) 18, 130
Sau96I GGNCC 3 cut(s) 53, 62, 158
ScrFI CCNGG 1 cut(s) 57
SetI ASST 2 cut(s) 47, 58
SexAI ACCWGGT 1 cut(s) 55
SfaNI GCATC 1 cut(s) 79
Sfr274I CTCGAG 1 cut(s) 170
SfuI TTCGAA 1 cut(s) 165
SinI GGWCC 2 cut(s) 53, 158
SlaI CTCGAG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 93
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 75
TaiI ACGT 1 cut(s) 47
TaqI TCGA 2 cut(s) 165, 171
TscAI CASTG 1 cut(s) 78
TspRI CASTG 1 cut(s) 78
VpaK11BI GGWCC 2 cut(s) 53, 158
XceI RCATGY 1 cut(s) 31
XhoI CTCGAG 1 cut(s) 170
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.