Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004943

Physical Location & Seq

Genomic Coordinates Forward (+)

21337861 .. 21343051

Gene Structure 5191 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G52580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2022 bp

ATGAGTATGGAAGCAACGGAGCTACAAGATCTCTCTGACGACGCCGATTACGCCGCTTCTCAACAACAAGGATCGGCTAGTATGATGCGTAGCGATAGCGGAAAGAGAAGTTTACAAAGTGAACACGAAGACGCTGTATTGATTTATTTGAAAGATAACGTTGCAATTCATCCTACACAGTTCGCATCTGAGAGGATTAGTGGTAGATTGAAGTTGACTAAGCAAGACTCTGTTCTCTTTCTGTCGTGGATTCCGTACAAGGGACAAACATCAAATGCAAAGCTATCAGAGAAAGACAGGAGTCTTTATACCATCACCGCGGTTCCGTTTACTGAAGTGAGGTCCATTAGGAGACACACTCCTGCTCTCGGATGGCAGTATGTAATTGTTGTCCTGTCTTCTGGACTTGCATTTCCACCTCTGTACTTCTACAATGGAGGAGTCAGGGAGTTTCTGGCCATGGTGAAGCAGCATGTTTTTCTTGCAAGGTCGTCAGAAGATCAAAATGTGTTCATTGTGAATGATTTTCAGAGTCCCTTGCAGAGAACTTTGTCTTCATTGGAGCTGCCAAGTTCACTGCCTGTAGCAAGTGGACAATCCGTATACCCATTAGACGGAGGTTCTTCTAGTGAAAATCAAAGGAGAACAAGTTCAGATGTTGGTAATAGAGTATCTAGTGTTTCCCAGTCTGGCTTCAGAAAGCAAAAGAGTCATGATCCAACTCGGGATCTTTCAATCCATCTACTAGAGAAGTTTTCTCTGGTTACCAAATTTGCTCGAGATACAACTACTCAGCTGTTTTCGGAAAACAATGGCTTTGGTTCCATTGACAAGAGATGGAATAACCAACCCGTACATAGTTATCCTGAAAAGTTGTCGAACATTGCTGAGGAAAAGCATCATGAAATCCGTCATAGTTATTCTGAAAATGACCTTCTCAAGGATGACGAAATTTCTTATATTGATGTCCCTGCTGATCCCTTAGAGTTTGATAAATTAAGTTTGATGTGGGGAAAACCAAGGCAACCACCAATGGGGCATAAAGAGTTCACAGCATTGTTGGATTCTGAAGGGCGAGTTGTGGAATCAAAAGCTCTTCGGGAGAGAGTTTTCTATGGAGGCATTGAGCACCAGTTGCGTAGAGAGGTATGGCCCTTTCTCTTGGGATATTATGCATATGACTCGACATATGCAGAGAGAGAATATCTTCGATCTGTCAAACGGATGGAATATGCAACATTGAAACAGCAGTGGCAGAGCATTTCCCCTGAACAAGCAAAAAGGTTCACAAAATATCGGGAGAGAAAAGGATTGATAGATAAAGATGTGGTAAGAACTGATAGGGCATTTGAATACTATGAAGGGGATGACAATCTACATGTCAATAGCATGCGTGATATTCTGTTGACCTACTCCTTCTACAATTTTGACCTGGGTTACTGCCAGGGAATGAGTGATTATCTGTCTCCTATCTTGTTCGTGATGGAGGATGAATCAGAATCTTTTTGGTGTTTCGTGGCACTGATGGAACGACTTGGACCCAACTTTAACCGTGACCAGAATGGGATGCATACTCAGCTCTTTGCACTCTCAAAGCTGGTAGAGTTGCTCGATAGCCCGCTACATAATTACTTTAAGGAGAATGACTGCTTGAATTACTTCTTCTGTTTCCGCTGGATTCTGATTCAGTTTAAAAGGGAATTTGAGTATGAGAAGACAATGCAGCTGTGGGAGGTGATGTGGACCCACTACCTCTCGGAACATTTTCACCTATATGTTTGTGTGGCGGTCTTGAAGCGATGCCGCAGCAAGATAATGGGAGAACAGATGGATTTTGACACTCTCTTAAAGTTCATCAATGAGCTGTCTGGGCATATTGATCTCGATTCAACAGTCAGAGATGCCGAAGCACTTTGCATAGAAGCTGGTGAAAATGGTGCTGCTAGCATTCCTCCAGGAACCCCTCCTTCTCTACCCCTTGATGACGGTACCTTATATCCTCAGGAAGATGATGTTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006886 GO:0008047 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015833 GO:0017016 GO:0017137 GO:0019899 GO:0030234 GO:0030695 GO:0031267 GO:0031338 GO:0032879 GO:0033036 GO:0033043 GO:0034613 GO:0042886 GO:0043085 GO:0043087 GO:0043547 GO:0044093 GO:0044424 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0060589 GO:0060627 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071705 GO:0090630 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

DUF3548 RabGAP-TBC

Protein Analysis

673

Amino Acids

77.67

Weight (kDa)

5.4

Isoelectric Point (pI)

54.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_RBD	PF12068	47 - 188	8.6e-17	Rab-binding domain (RBD)
RabGAP-TBC	PF00566	377 - 606	1.3e-47	Rab-GTPase-TBC domain
RabGap-TBC_2	PF23436	464 - 621	1.7e-13	RabGAP TBC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52580 AT5G52580 AT5G52580
fragaria_vesca	FvH4_1g02680
malus_domestica	MD02G1027400.v1.1 MD15G1168800.v1.1
prunus_persica	Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1
pyrus_communis	pycom02g02230 pycom15g15050
rosa_chinensis	RchiOBHm_Chr2g0087771 RchiOBHm_Chr7g0194011
rosa_laevigata	RLG00000004223 RLG00000015897
rosa_multiflora	Rmu_sc0021871.1_g000005
rosa_roxburghii	Rroxscaffold_2G00153250 Rroxscaffold_3G00261620
rosa_rugosa	Rorug01G0478700 Rorug07G0013200
rosa_samantha	Rh2AG032900 Rh2CG032700 Rh2DG032500 Rh7AG140400 Rh7BG140900 Rh7CG144400 Rh7DG141700
rosa_wichuraiana	Rw2G002560 Rw7G011980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1988
AccB1I	GGYRCC	1 cut(s)	1988
AccI	GTMKAC	1 cut(s)	603
AccII	CGCG	1 cut(s)	320
AciI	CCGC	8 cut(s)	54, 99, 318, 320, 1619, 1672, 1787, 1804
AclI	AACGTT	1 cut(s)	159
AclWI	GGATC	4 cut(s)	79, 710, 735, 971
AcoI	YGGCCR	1 cut(s)	456
AcsI	RAATTY	3 cut(s)	770, 951, 1700
AcuI	CTGAAG	3 cut(s)	354, 679, 1089
AcyI	GRCGYC	1 cut(s)	42
AfaI	GTAC	4 cut(s)	257, 425, 855, 1990
AfiI	CCNNNNNNNGG	5 cut(s)	260, 368, 614, 940, 1034
AflIII	ACRYGT	1 cut(s)	1378
AgsI	TTSAA	8 cut(s)	151, 211, 735, 1243, 1352, 1654, 1795, 1890
AjnI	CCWGG	3 cut(s)	1431, 1443, 1954
AloI	GAACNNNNNNTCC	2 cut(s)	634, 666
Alw21I	GWGCWC	1 cut(s)	1131
Alw26I	GTCTC	2 cut(s)	346, 1470
AlwI	GGATC	4 cut(s)	79, 710, 735, 971
Ama87I	CYCGRG	2 cut(s)	723, 777
AoxI	GGCC	2 cut(s)	456, 1151
ApeKI	GCWGC	5 cut(s)	469, 565, 1723, 1806, 1940
ApoI	RAATTY	3 cut(s)	770, 951, 1700
Asp700I	GAANNNNTTC	4 cut(s)	649, 1206, 1658, 1764
Asp718I	GGTACC	1 cut(s)	1988
AspS9I	GGNCC	4 cut(s)	342, 1152, 1538, 1743
AsuHPI	GGTGA	5 cut(s)	307, 475, 1747, 1760, 1940
AsuNHI	GCTAGC	1 cut(s)	1943
AvaI	CYCGRG	2 cut(s)	723, 777
AvaII	GGWCC	3 cut(s)	342, 1538, 1743
AxyI	CCTNAGG	1 cut(s)	2001
BalI	TGGCCA	1 cut(s)	458
BanI	GGYRCC	1 cut(s)	1988
BbsI	GAAGAC	4 cut(s)	135, 390, 546, 1721
Bbv12I	GWGCWC	1 cut(s)	1131
BbvCI	CCTCAGC	1 cut(s)	888
BbvI	GCAGC	5 cut(s)	481, 552, 1735, 1818, 1927
BccI	CCATC	8 cut(s)	320, 366, 747, 831, 1219, 1477, 1519, 1822
BcgI	CGANNNNNNTGC	2 cut(s)	1164, 1198
BciT130I	CCWGG	3 cut(s)	1433, 1445, 1956
BcoDI	GTCTC	2 cut(s)	346, 1470
BfaI	CTAG	5 cut(s)	78, 627, 675, 746, 1944
BfmI	CTRYAG	1 cut(s)	582
BglII	AGATCT	1 cut(s)	28
BisI	GCNGC	7 cut(s)	54, 470, 566, 1724, 1804, 1807, 1941
BlsI	GCNGC	7 cut(s)	55, 471, 567, 1725, 1805, 1808, 1942
Bme1390I	CCNGG	3 cut(s)	1433, 1445, 1956
Bme18I	GGWCC	3 cut(s)	342, 1538, 1743
BmeT110I	CYCGRG	2 cut(s)	723, 777
BmgT120I	GGNCC	4 cut(s)	342, 1152, 1538, 1743
BmiI	GGNNCC	6 cut(s)	324, 823, 1540, 1745, 1960, 1990
BmrFI	CCNGG	3 cut(s)	1433, 1445, 1956
BmrI	ACTGGG	1 cut(s)	679
BmsI	GCATC	6 cut(s)	75, 194, 907, 1557, 1790, 1891
BmtI	GCTAGC	1 cut(s)	1947
BmuI	ACTGGG	1 cut(s)	679
BoxI	GACNNNNGTC	1 cut(s)	300
BpiI	GAAGAC	4 cut(s)	135, 390, 546, 1721
BplI	GAGNNNNNCTC	2 cut(s)	343, 375
BpmI	CTGGAG	1 cut(s)	1938
Bpu10I	CCTNAGC	1 cut(s)	888
BpuEI	CTTGAG	1 cut(s)	923
BsaBI	GATNNNNATC	1 cut(s)	1371
BsaHI	GRCGYC	1 cut(s)	42
BsaJI	CCNNGG	5 cut(s)	318, 459, 1019, 1432, 1444
BsaXI	ACNNNNNCTCC	4 cut(s)	634, 664, 1724, 1754
Bsc4I	CCNNNNNNNGG	5 cut(s)	260, 368, 614, 940, 1034
Bse1I	ACTGG	2 cut(s)	685, 1132
Bse21I	CCTNAGG	1 cut(s)	2001
Bse3DI	GCAATG	1 cut(s)	882
Bse8I	GATNNNNATC	1 cut(s)	1371
BseBI	CCWGG	3 cut(s)	1433, 1445, 1956
BseDI	CCNNGG	5 cut(s)	318, 459, 1019, 1432, 1444
BseGI	GGATG	7 cut(s)	169, 377, 949, 1230, 1372, 1495, 1572
BseJI	GATNNNNATC	1 cut(s)	1371
BseLI	CCNNNNNNNGG	5 cut(s)	260, 368, 614, 940, 1034
BseMI	GCAATG	1 cut(s)	882
BseMII	CTCAG	5 cut(s)	180, 806, 879, 1589, 2015
BseNI	ACTGG	2 cut(s)	685, 1132
BseRI	GAGGAG	1 cut(s)	453
BseXI	GCAGC	5 cut(s)	481, 552, 1735, 1818, 1927
Bsh1236I	CGCG	1 cut(s)	320
BshFI	GGCC	2 cut(s)	458, 1153
BshNI	GGYRCC	1 cut(s)	1988
BsiHKAI	GWGCWC	1 cut(s)	1131
BsiHKCI	CYCGRG	2 cut(s)	723, 777
BslFI	GGGAC	3 cut(s)	276, 519, 953
BslI	CCNNNNNNNGG	5 cut(s)	260, 368, 614, 940, 1034
BsmAI	GTCTC	2 cut(s)	346, 1470
BsmFI	GGGAC	3 cut(s)	276, 519, 953
BsmI	GAATGC	1 cut(s)	1947
BsnI	GGCC	2 cut(s)	458, 1153
BsoBI	CYCGRG	2 cut(s)	723, 777
Bsp1286I	GDGCHC	1 cut(s)	1131
Bsp143I	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
Bsp19I	CCATGG	1 cut(s)	459
BspACI	CCGC	8 cut(s)	54, 99, 318, 320, 1619, 1672, 1787, 1804
BspANI	GGCC	2 cut(s)	458, 1153
BspCNI	CTCAG	5 cut(s)	181, 805, 880, 1588, 2014
BspFNI	CGCG	1 cut(s)	320
BspHI	TCATGA	2 cut(s)	712, 901
BspLI	GGNNCC	6 cut(s)	324, 823, 1540, 1745, 1960, 1990
BspOI	GCTAGC	1 cut(s)	1947
BspPI	GGATC	4 cut(s)	79, 710, 735, 971
BspQI	GCTCTTC	1 cut(s)	1101
BspT107I	GGYRCC	1 cut(s)	1988
BsrDI	GCAATG	1 cut(s)	882
BsrI	ACTGG	2 cut(s)	685, 1132
BssECI	CCNNGG	5 cut(s)	318, 459, 1019, 1432, 1444
BssMI	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
BssNAI	GTATAC	1 cut(s)	604
BssNI	GRCGYC	1 cut(s)	42
BssT1I	CCWWGG	2 cut(s)	459, 1019
Bst1107I	GTATAC	1 cut(s)	604
Bst2UI	CCWGG	3 cut(s)	1433, 1445, 1956
Bst4CI	ACNGT	4 cut(s)	180, 1553, 1894, 1988
Bst6I	CTCTTC	1 cut(s)	1101
BstACI	GRCGYC	1 cut(s)	42
BstAPI	GCANNNNNTGC	1 cut(s)	1135
BstC8I	GCNNGC	3 cut(s)	1391, 1619, 1945
BstDEI	CTNAG	7 cut(s)	189, 219, 792, 888, 982, 1575, 2001
BstDSI	CCRYGG	2 cut(s)	318, 459
BstEII	GGTNACC	1 cut(s)	763
BstENI	CCTNNNNNAGG	1 cut(s)	938
BstF5I	GGATG	7 cut(s)	169, 377, 949, 1230, 1372, 1495, 1572
BstFNI	CGCG	1 cut(s)	320
BstKTI	GATC	8 cut(s)	31, 74, 502, 718, 730, 979, 1214, 1882
BstMAI	GTCTC	2 cut(s)	346, 1470
BstMBI	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
BstMWI	GCNNNNNNNGC	4 cut(s)	50, 1135, 1576, 1870
BstNI	CCWGG	3 cut(s)	1433, 1445, 1956
BstNSI	RCATGY	3 cut(s)	476, 1382, 1393
BstPAI	GACNNNNGTC	1 cut(s)	300
BstPI	GGTNACC	1 cut(s)	763
BstSCI	CCNGG	3 cut(s)	1431, 1443, 1954
BstSFI	CTRYAG	1 cut(s)	582
BstUI	CGCG	1 cut(s)	320
BstV1I	GCAGC	5 cut(s)	481, 552, 1735, 1818, 1927
BstV2I	GAAGAC	4 cut(s)	135, 390, 546, 1721
BstX2I	RGATCY	2 cut(s)	28, 727
BstYI	RGATCY	2 cut(s)	28, 727
BstZ17I	GTATAC	1 cut(s)	604
Bsu36I	CCTNAGG	1 cut(s)	2001
BsuRI	GGCC	2 cut(s)	458, 1153
BtgI	CCRYGG	2 cut(s)	318, 459
BtgZI	GCGATG	1 cut(s)	1813
BtsCI	GGATG	7 cut(s)	169, 377, 949, 1230, 1372, 1495, 1572
BtsI	GCAGTG	2 cut(s)	575, 1256
BtsIMutI	CAGTG	3 cut(s)	575, 1256, 1520
Cac8I	GCNNGC	3 cut(s)	1391, 1619, 1945
CciI	TCATGA	2 cut(s)	712, 901
Cfr13I	GGNCC	4 cut(s)	342, 1152, 1538, 1743
Cfr42I	CCGCGG	1 cut(s)	321
CseI	GACGC	2 cut(s)	50, 140
Csp6I	GTAC	4 cut(s)	256, 424, 854, 1989
CviAII	CATG	6 cut(s)	460, 473, 713, 902, 1379, 1390
CviQI	GTAC	4 cut(s)	256, 424, 854, 1989
DdeI	CTNAG	7 cut(s)	189, 219, 792, 888, 982, 1575, 2001
DpnI	GATC	8 cut(s)	30, 73, 501, 717, 729, 978, 1213, 1881
DpnII	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
DraI	TTTAAA	1 cut(s)	1693
EaeI	YGGCCR	1 cut(s)	456
Eam1104I	CTCTTC	1 cut(s)	1101
EarI	CTCTTC	1 cut(s)	1101
Eco130I	CCWWGG	2 cut(s)	459, 1019
Eco47I	GGWCC	3 cut(s)	342, 1538, 1743
Eco57I	CTGAAG	3 cut(s)	354, 679, 1089
Eco81I	CCTNAGG	1 cut(s)	2001
Eco88I	CYCGRG	2 cut(s)	723, 777
Eco91I	GGTNACC	1 cut(s)	763
EcoNI	CCTNNNNNAGG	1 cut(s)	938
EcoO65I	GGTNACC	1 cut(s)	763
EcoRII	CCWGG	3 cut(s)	1431, 1443, 1954
EcoT14I	CCWWGG	2 cut(s)	459, 1019
EcoT22I	ATGCAT	2 cut(s)	1177, 1572
ErhI	CCWWGG	2 cut(s)	459, 1019
FaeI	CATG	6 cut(s)	463, 476, 716, 905, 1382, 1393
FaqI	GGGAC	3 cut(s)	276, 519, 953
FatI	CATG	6 cut(s)	459, 472, 712, 901, 1378, 1389
FauI	CCCGC	1 cut(s)	1626
FauNDI	CATATG	2 cut(s)	1177, 1189
FblI	GTMKAC	1 cut(s)	603
Fnu4HI	GCNGC	7 cut(s)	54, 470, 566, 1724, 1804, 1807, 1941
FokI	GGATG	7 cut(s)	156, 384, 956, 1237, 1379, 1502, 1579
Fsp4HI	GCNGC	7 cut(s)	54, 470, 566, 1724, 1804, 1807, 1941
FspBI	CTAG	5 cut(s)	78, 627, 675, 746, 1944
GluI	GCNGC	7 cut(s)	54, 470, 566, 1724, 1804, 1807, 1941
GsuI	CTGGAG	1 cut(s)	1938
HaeIII	GGCC	2 cut(s)	458, 1153
HgaI	GACGC	2 cut(s)	50, 140
Hin1I	GRCGYC	1 cut(s)	42
Hin1II	CATG	6 cut(s)	463, 476, 716, 905, 1382, 1393
HincII	GTYRAC	2 cut(s)	216, 1407
HindII	GTYRAC	2 cut(s)	216, 1407
HphI	GGTGA	5 cut(s)	307, 475, 1747, 1760, 1940
Hpy99I	CGWCG	1 cut(s)	44
HpyAV	CCTTC	5 cut(s)	944, 1064, 1355, 1426, 1977
HpyCH4III	ACNGT	4 cut(s)	180, 1553, 1894, 1988
HpyCH4IV	ACGT	1 cut(s)	159
HpyF10VI	GCNNNNNNNGC	4 cut(s)	50, 1135, 1576, 1870
HpyF3I	CTNAG	7 cut(s)	189, 219, 792, 888, 982, 1575, 2001
HpySE526I	ACGT	1 cut(s)	159
Hsp92I	GRCGYC	1 cut(s)	42
Hsp92II	CATG	6 cut(s)	463, 476, 716, 905, 1382, 1393
KpnI	GGTACC	1 cut(s)	1992
KspI	CCGCGG	1 cut(s)	321
Kzo9I	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
LguI	GCTCTTC	1 cut(s)	1101
LmnI	GCTCC	2 cut(s)	19, 562
Lsp1109I	GCAGC	5 cut(s)	481, 552, 1735, 1818, 1927
LweI	GCATC	6 cut(s)	75, 194, 907, 1557, 1790, 1891
MaeI	CTAG	5 cut(s)	78, 627, 675, 746, 1944
MaeII	ACGT	1 cut(s)	159
MaeIII	GTNAC	3 cut(s)	763, 1436, 1553
MalI	GATC	8 cut(s)	30, 73, 501, 717, 729, 978, 1213, 1881
MboI	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
MflI	RGATCY	2 cut(s)	28, 727
MhlI	GDGCHC	1 cut(s)	1131
MlsI	TGGCCA	1 cut(s)	458
MluCI	AATT	9 cut(s)	165, 384, 770, 951, 995, 1423, 1627, 1654, 1700
MluNI	TGGCCA	1 cut(s)	458
MlyI	GAGTC	6 cut(s)	221, 310, 450, 541, 718, 1175
MmeI	TCCRAC	2 cut(s)	743, 1041
Mox20I	TGGCCA	1 cut(s)	458
Mph1103I	ATGCAT	2 cut(s)	1177, 1572
MroXI	GAANNNNTTC	4 cut(s)	649, 1206, 1658, 1764
MscI	TGGCCA	1 cut(s)	458
MseI	TTAA	5 cut(s)	998, 1548, 1635, 1692, 1847
MslI	CAYNNNNRTG	1 cut(s)	1773
Msp20I	TGGCCA	1 cut(s)	458
MspA1I	CMGCKG	4 cut(s)	320, 796, 1674, 1726
MspR9I	CCNGG	3 cut(s)	1433, 1445, 1956
Mva1269I	GAATGC	1 cut(s)	1947
MvaI	CCWGG	3 cut(s)	1433, 1445, 1956
MvnI	CGCG	1 cut(s)	320
MwoI	GCNNNNNNNGC	4 cut(s)	50, 1135, 1576, 1870
NcoI	CCATGG	1 cut(s)	459
NdeI	CATATG	2 cut(s)	1177, 1189
NdeII	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
NheI	GCTAGC	1 cut(s)	1943
NlaIII	CATG	6 cut(s)	463, 476, 716, 905, 1382, 1393
NlaIV	GGNNCC	6 cut(s)	324, 823, 1540, 1745, 1960, 1990
NmuCI	GTSAC	1 cut(s)	1553
NsiI	ATGCAT	2 cut(s)	1177, 1572
NspI	RCATGY	3 cut(s)	476, 1382, 1393
PaeI	GCATGC	1 cut(s)	1393
PaeR7I	CTCGAG	1 cut(s)	777
PagI	TCATGA	2 cut(s)	712, 901
PciI	ACATGT	1 cut(s)	1378
PciSI	GCTCTTC	1 cut(s)	1101
PcsI	WCGNNNNNNNCGW	1 cut(s)	251
PctI	GAATGC	1 cut(s)	1947
PdmI	GAANNNNTTC	4 cut(s)	649, 1206, 1658, 1764
PfeI	GAWTC	8 cut(s)	250, 1064, 1085, 1493, 1499, 1678, 1684, 1886
PfoI	TCCNGGA	1 cut(s)	1954
PkrI	GCNGC	7 cut(s)	55, 471, 567, 1725, 1805, 1808, 1942
PleI	GAGTC	6 cut(s)	221, 309, 449, 540, 717, 1175
PpsI	GAGTC	6 cut(s)	221, 309, 449, 540, 717, 1175
PscI	ACATGT	1 cut(s)	1378
PshAI	GACNNNNGTC	1 cut(s)	300
Psp1406I	AACGTT	1 cut(s)	159
Psp6I	CCWGG	3 cut(s)	1431, 1443, 1954
PspEI	GGTNACC	1 cut(s)	763
PspGI	CCWGG	3 cut(s)	1431, 1443, 1954
PspN4I	GGNNCC	6 cut(s)	324, 823, 1540, 1745, 1960, 1990
PspPI	GGNCC	4 cut(s)	342, 1152, 1538, 1743
PsuI	RGATCY	2 cut(s)	28, 727
PvuII	CAGCTG	2 cut(s)	796, 1726
RsaI	GTAC	4 cut(s)	257, 425, 855, 1990
RsaNI	GTAC	4 cut(s)	256, 424, 854, 1989
RseI	CAYNNNNRTG	1 cut(s)	1773
SacII	CCGCGG	1 cut(s)	321
SapI	GCTCTTC	1 cut(s)	1101
SaqAI	TTAA	5 cut(s)	998, 1548, 1635, 1692, 1847
SatI	GCNGC	7 cut(s)	54, 470, 566, 1724, 1804, 1807, 1941
Sau3AI	GATC	8 cut(s)	28, 71, 499, 715, 727, 976, 1211, 1879
Sau96I	GGNCC	4 cut(s)	342, 1152, 1538, 1743
SchI	GAGTC	6 cut(s)	221, 310, 450, 541, 718, 1175
ScrFI	CCNGG	3 cut(s)	1433, 1445, 1956
SduI	GDGCHC	1 cut(s)	1131
SfaNI	GCATC	6 cut(s)	75, 194, 907, 1557, 1790, 1891
SfcI	CTRYAG	1 cut(s)	582
Sfr274I	CTCGAG	1 cut(s)	777
Sfr303I	CCGCGG	1 cut(s)	321
SgrBI	CCGCGG	1 cut(s)	321
SinI	GGWCC	3 cut(s)	342, 1538, 1743
SlaI	CTCGAG	1 cut(s)	777
SmiMI	CAYNNNNRTG	1 cut(s)	1773
SmlI	CTYRAG	2 cut(s)	777, 938
SmoI	CTYRAG	2 cut(s)	777, 938
SphI	GCATGC	1 cut(s)	1393
Sse9I	AATT	9 cut(s)	165, 384, 770, 951, 995, 1423, 1627, 1654, 1700
SsiI	CCGC	8 cut(s)	54, 99, 318, 320, 1619, 1672, 1787, 1804
SspMI	CTAG	5 cut(s)	78, 627, 675, 746, 1944
StyD4I	CCNGG	3 cut(s)	1431, 1443, 1954
StyI	CCWWGG	2 cut(s)	459, 1019
TaaI	ACNGT	4 cut(s)	180, 1553, 1894, 1988
TaiI	ACGT	1 cut(s)	162
TaqI	TCGA	6 cut(s)	778, 878, 1184, 1210, 1611, 1884
TasI	AATT	9 cut(s)	165, 384, 770, 951, 995, 1423, 1627, 1654, 1700
TatI	WGTACW	1 cut(s)	423
TauI	GCSGC	2 cut(s)	56, 1806
TfiI	GAWTC	8 cut(s)	250, 1064, 1085, 1493, 1499, 1678, 1684, 1886
Tru1I	TTAA	5 cut(s)	998, 1548, 1635, 1692, 1847
Tru9I	TTAA	5 cut(s)	998, 1548, 1635, 1692, 1847
TscAI	CASTG	3 cut(s)	582, 1256, 1527
TseFI	GTSAC	1 cut(s)	1553
TseI	GCWGC	5 cut(s)	469, 565, 1723, 1806, 1940
Tsp45I	GTSAC	1 cut(s)	1553
TspDTI	ATGAA	7 cut(s)	158, 502, 546, 918, 1374, 1506, 1843
TspGWI	ACGGA	7 cut(s)	32, 243, 315, 589, 630, 899, 1237
TspRI	CASTG	3 cut(s)	582, 1256, 1527
VpaK11BI	GGWCC	3 cut(s)	342, 1538, 1743
XagI	CCTNNNNNAGG	1 cut(s)	938
XapI	RAATTY	3 cut(s)	770, 951, 1700
XceI	RCATGY	3 cut(s)	476, 1382, 1393
XhoI	CTCGAG	1 cut(s)	777
XmiI	GTMKAC	1 cut(s)	603
XmnI	GAANNNNTTC	4 cut(s)	649, 1206, 1658, 1764
XspI	CTAG	5 cut(s)	78, 627, 675, 746, 1944
Zsp2I	ATGCAT	2 cut(s)	1177, 1572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.