Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004943

Physical Location & Seq

Genomic Coordinates Forward (+)

2080879 .. 2085695

Gene Structure 4817 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1027400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1980 bp

ATGCAAGAACCAGAGCTCCACGATCTCTCCGACGACGCCGATTACGCCGCGTCGCTGCAACAAGGCTCGGCGAGCATGATGCGGAGCGATAGCGGGAAGCACAGCTCGTCGAGCGAGCATGACGGTGCCGAGGTGGTTTTCTTGAAAGACAATGTCGCCATCCACCCCACTCAGTTCGTCTCCGAGAGGATTAGTGGAAGATTGAGGCTAATTAAGCAGGGCTCTTCTCTTTTCATGACATGGATTCCGTACAACGGCCAAAACTCGAACGCTAGGGTGTCTGAGAAAGATAGAAATCTTTATACCATAAGGTCTGTGCCATTAACTGAGGTGCGGTCGATTCGTCGCCACACTCCGGCGCTTGGATGGCAGTACATTATCGTTGTTTTGGCTACAGGACTTGCATTTCCTCCTCTGTACTTCTACAATGGAGGAGTCAAAGAGTTCCTTGCTACAATAAAGCAACATGTTTTTCTTGTGAGGTCAGCTGAAGATGCAAATGTATTTCTGGTGAATGATTTTCAAAATCCTCTCCAGAGAACTCTATCATCTTTGGAGTTGCCTAGGGCTCTTTCTGTTGCAAGTGGGCAATCTACATTAGTCTCAGTTGGTGAGACCTCTTCAGATGAAAACCAGGAGAGAACAAGCGGGTATGTTCAAGAAGGAAGTATAAGCATTTCTCAATACAATGAGAGGCAAAGGCAAAGGGTTCATGATCCTGCACGAGATCTCTCAATTCAAGTGTTGGAAAAGTTTTCTCTCGTGACCAAATTTGCTCGAGAAACAACTTCTCAACTTTTCCGTGATAATCATAGTAACGGATATGGTGCTATAGAAAGGAGAAGTAATAACCAGTCTCCCATTGATTACCCTCAGAAGGCATCCCATGATTCAAACAGAGCCCCTGAGAAAAGTCCTGTCCCCCCAGATTCCCTGGAGTTTGACAAAGAATCAATGGTATGGGGAAAACCACGACAGCCACCTTTGGGCTCTGATGAGTGGGAAGCTTTCTTTGATTCTGAAGGAAGGATCATCGACTCAGAAGCTATCAGAAAGAGAATATTTTATGGGGGAGTTGAGCACAACCTACGGAAAGAGGTTTGGCCCTTCTTGTTGGGATACCATGCATTTCATTCAACAGATGCTGAGAGGGAATACCTTAGGGCCGTTAAAAAGTCAGAATTTGAAACCATAAAGAAGCAATGGCAGGGTATCTCCCCTGAACAGGCCAAAAGATTTACAAAATTCAGGGAGAGAAAGGGCCTCATTGAGAAAGATGTGGTAAGGACTGACAGATCTCTCTCCTACTATGACGGGGATGATAATCCAAATGTGAATCTGTTACGTGATATACTCTTAACGTACTCATTTTACAACTTCGATCTTGGTTACTGTCAGGGTATGAGTGATCTGCTGTCCCCAATATTGTATGTAATGGAGGATGAGTCCGAATCGTTTTGGTGTTTTGTTTCACTGATGGAGCGCCTTGGGCCCAATTTCAACCGTGACCAAAGTGGCATGCACTCTCAACTTTTTGCAATAACTAAGTTGGTGGAGTTGTTAGACGCTCCGTTGCACAATCACTTTACTCAGAAGGACTGCTTGAATTACTTCTTTTGTTTCCGCTGGGTTCTAATTCAATTTAAAAGGGAATTTGAATTCGACAAAACAATGCGCTTGTGGGAGGTATTGTGGACACATTATCCGTCTGAGCACCTGCACCTGTACGTCTGTGTGGCGATATTGAAGCGACACCGTAGTAAGATAATTGGGGAGCAAATGGACTTCGACACTCTATTGAAGTTCATCAATGAACTGAGCGGTCATATAGATCTTGATGCAGTGGTCAGGGATGCAGAGGCTCTGTGCATATGTGCCGGTGAGAATGGTGCTGCTTCCATTCCACCGGGAACTCCGCCGTCATTGCCTGTTGATGATGTTTTATTGTACCCTCAACAAGAACTAGACGACATATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006886 GO:0008047 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015833 GO:0017016 GO:0017137 GO:0019899 GO:0030234 GO:0030695 GO:0031267 GO:0031338 GO:0032879 GO:0033036 GO:0033043 GO:0034613 GO:0042886 GO:0043085 GO:0043087 GO:0043547 GO:0044093 GO:0044424 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0060589 GO:0060627 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071705 GO:0090630 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

DUF3548 RabGAP-TBC

Protein Analysis

660

Amino Acids

75.59

Weight (kDa)

5.57

Isoelectric Point (pI)

47.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_RBD	PF12068	45 - 186	1.5e-18	Rab-binding domain (RBD)
RabGAP-TBC	PF00566	362 - 590	2e-51	Rab-GTPase-TBC domain
RabGap-TBC_2	PF23436	449 - 605	2.3e-17	RabGAP TBC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52580 AT5G52580 AT5G52580
fragaria_vesca	FvH4_1g02680
malus_domestica	MD02G1027400.v1.1 MD15G1168800.v1.1
prunus_persica	Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1
pyrus_communis	pycom02g02230 pycom15g15050
rosa_chinensis	RchiOBHm_Chr2g0087771 RchiOBHm_Chr7g0194011
rosa_laevigata	RLG00000004223 RLG00000015897
rosa_multiflora	Rmu_sc0021871.1_g000005
rosa_roxburghii	Rroxscaffold_2G00153250 Rroxscaffold_3G00261620
rosa_rugosa	Rorug01G0478700 Rorug07G0013200
rosa_samantha	Rh2AG032900 Rh2CG032700 Rh2DG032500 Rh7AG140400 Rh7BG140900 Rh7CG144400 Rh7DG141700
rosa_wichuraiana	Rw2G002560 Rw7G011980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1725
Acc36I	ACCTGC	1 cut(s)	1725
AccB1I	GGYRCC	1 cut(s)	125
AccBSI	CCGCTC	1 cut(s)	1821
AccII	CGCG	1 cut(s)	50
AciI	CCGC	8 cut(s)	48, 82, 93, 334, 648, 1624, 1821, 1916
AclWI	GGATC	2 cut(s)	710, 1037
AcoI	YGGCCR	1 cut(s)	256
AcsI	RAATTY	5 cut(s)	770, 1181, 1244, 1652, 1658
AcuI	CTGAAG	3 cut(s)	510, 606, 1041
AcyI	GRCGYC	1 cut(s)	36
AfaI	GTAC	6 cut(s)	251, 374, 419, 1364, 1727, 1949
AfiI	CCNNNNNNNGG	6 cut(s)	254, 355, 362, 877, 986, 1225
AflIII	ACRYGT	1 cut(s)	466
AjnI	CCWGG	2 cut(s)	633, 933
AloI	GAACNNNNNNTCC	1 cut(s)	32
AluBI	AGCT	5 cut(s)	16, 105, 488, 1007, 1046
AluI	AGCT	5 cut(s)	16, 105, 488, 1007, 1046
Alw21I	GWGCWC	3 cut(s)	18, 1083, 1716
Alw26I	GTCTC	4 cut(s)	184, 607, 608, 861
AlwI	GGATC	2 cut(s)	710, 1037
AlwNI	CAGNNNCTG	1 cut(s)	1145
Ama87I	CYCGRG	1 cut(s)	777
AoxI	GGCC	6 cut(s)	256, 1103, 1164, 1227, 1261, 1490
ApaI	GGGCCC	1 cut(s)	1494
ApeKI	GCWGC	2 cut(s)	55, 1892
ApoI	RAATTY	5 cut(s)	770, 1181, 1244, 1652, 1658
Asp700I	GAANNNNTTC	1 cut(s)	1610
AspA2I	CCTAGG	1 cut(s)	563
AspLEI	GCGC	3 cut(s)	361, 1485, 1677
AspS9I	GGNCC	5 cut(s)	1104, 1164, 1261, 1490, 1491
AsuC2I	CCSGG	1 cut(s)	1908
AsuHPI	GGTGA	3 cut(s)	523, 623, 1892
AvaI	CYCGRG	1 cut(s)	777
AvrII	CCTAGG	1 cut(s)	563
AxyI	CCTNAGG	1 cut(s)	1160
BaeGI	GKGCMC	1 cut(s)	1494
BanI	GGYRCC	1 cut(s)	125
BanII	GRGCYC	6 cut(s)	18, 224, 571, 904, 992, 1494
BauI	CACGAG	2 cut(s)	723, 761
Bbv12I	GWGCWC	3 cut(s)	18, 1083, 1716
BbvI	GCAGC	2 cut(s)	42, 1879
BccI	CCATC	3 cut(s)	167, 360, 1471
BceAI	ACGGC	3 cut(s)	271, 1151, 1903
BcgI	CGANNNNNNTGC	2 cut(s)	61, 95
BciT130I	CCWGG	2 cut(s)	635, 935
BciVI	GTATCC	1 cut(s)	1112
BcnI	CCSGG	1 cut(s)	1908
BcoDI	GTCTC	4 cut(s)	184, 607, 608, 861
BfaI	CTAG	3 cut(s)	273, 564, 1964
BfmI	CTRYAG	2 cut(s)	393, 831
BfoI	RGCGCY	2 cut(s)	362, 1486
BfuAI	ACCTGC	1 cut(s)	1725
BfuI	GTATCC	1 cut(s)	1112
BglII	AGATCT	3 cut(s)	727, 1295, 1831
BisI	GCNGC	3 cut(s)	48, 56, 1893
BlnI	CCTAGG	1 cut(s)	563
BlsI	GCNGC	3 cut(s)	49, 57, 1894
Bme1390I	CCNGG	3 cut(s)	635, 935, 1908
BmeT110I	CYCGRG	1 cut(s)	777
BmgT120I	GGNCC	5 cut(s)	1104, 1164, 1261, 1490, 1491
BmiI	GGNNCC	2 cut(s)	127, 1492
BmrFI	CCNGG	3 cut(s)	635, 935, 1908
BmsI	GCATC	6 cut(s)	69, 484, 890, 1132, 1828, 1843
BpmI	CTGGAG	2 cut(s)	518, 956
BpuMI	CCSGG	1 cut(s)	1908
BsaAI	YACGTR	1 cut(s)	1346
BsaBI	GATNNNNATC	2 cut(s)	294, 1323
BsaHI	GRCGYC	1 cut(s)	36
BsaI	GGTCTC	1 cut(s)	608
BsaJI	CCNNGG	4 cut(s)	129, 563, 933, 1486
BsaXI	ACNNNNNCTCC	7 cut(s)	11, 30, 41, 1430, 1460, 1676, 1706
Bsc4I	CCNNNNNNNGG	6 cut(s)	254, 355, 362, 877, 986, 1225
Bse118I	RCCGGY	1 cut(s)	1877
Bse1I	ACTGG	1 cut(s)	853
Bse21I	CCTNAGG	1 cut(s)	1160
Bse3DI	GCAATG	2 cut(s)	1208, 1922
Bse8I	GATNNNNATC	2 cut(s)	294, 1323
BseBI	CCWGG	2 cut(s)	635, 935
BseDI	CCNNGG	4 cut(s)	129, 563, 933, 1486
BseGI	GGATG	6 cut(s)	159, 371, 881, 1324, 1447, 1858
BseJI	GATNNNNATC	2 cut(s)	294, 1323
BseLI	CCNNNNNNNGG	6 cut(s)	254, 355, 362, 877, 986, 1225
BseMI	GCAATG	2 cut(s)	1208, 1922
BseNI	ACTGG	1 cut(s)	853
BseRI	GAGGAG	2 cut(s)	402, 447
BseSI	GKGCMC	1 cut(s)	1494
BseXI	GCAGC	2 cut(s)	42, 1879
BseYI	CCCAGC	1 cut(s)	1626
BsgI	GTGCAG	2 cut(s)	705, 1703
Bsh1236I	CGCG	1 cut(s)	50
Bsh1285I	CGRYCG	1 cut(s)	338
BshFI	GGCC	6 cut(s)	258, 1105, 1166, 1229, 1263, 1492
BshNI	GGYRCC	1 cut(s)	125
BsiEI	CGRYCG	1 cut(s)	338
BsiHKAI	GWGCWC	3 cut(s)	18, 1083, 1716
BsiHKCI	CYCGRG	1 cut(s)	777
BsiSI	CCGG	3 cut(s)	356, 1878, 1907
BslFI	GGGAC	2 cut(s)	905, 1402
BslI	CCNNNNNNNGG	6 cut(s)	254, 355, 362, 877, 986, 1225
BsmAI	GTCTC	4 cut(s)	184, 607, 608, 861
BsmBI	CGTCTC	1 cut(s)	184
BsmFI	GGGAC	2 cut(s)	905, 1402
BsnI	GGCC	6 cut(s)	258, 1105, 1166, 1229, 1263, 1492
Bso31I	GGTCTC	1 cut(s)	608
BsoBI	CYCGRG	1 cut(s)	777
Bsp120I	GGGCCC	1 cut(s)	1490
Bsp1286I	GDGCHC	8 cut(s)	18, 224, 571, 904, 992, 1083, 1494, 1716
Bsp143I	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
BspACI	CCGC	8 cut(s)	48, 82, 93, 334, 648, 1624, 1821, 1916
BspANI	GGCC	6 cut(s)	258, 1105, 1166, 1229, 1263, 1492
BspFNI	CGCG	1 cut(s)	50
BspHI	TCATGA	2 cut(s)	234, 712
BspLI	GGNNCC	2 cut(s)	127, 1492
BspMI	ACCTGC	1 cut(s)	1725
BspPI	GGATC	2 cut(s)	710, 1037
BspQI	GCTCTTC	1 cut(s)	229
BspT107I	GGYRCC	1 cut(s)	125
BspTNI	GGTCTC	1 cut(s)	608
BsrBI	CCGCTC	1 cut(s)	1821
BsrDI	GCAATG	2 cut(s)	1208, 1922
BsrFI	RCCGGY	1 cut(s)	1877
BsrI	ACTGG	1 cut(s)	853
BssAI	RCCGGY	1 cut(s)	1877
BssECI	CCNNGG	4 cut(s)	129, 563, 933, 1486
BssMI	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
BssNI	GRCGYC	1 cut(s)	36
BssSI	CACGAG	2 cut(s)	723, 761
BssT1I	CCWWGG	2 cut(s)	563, 1486
Bst2BI	CACGAG	2 cut(s)	723, 761
Bst2UI	CCWGG	2 cut(s)	635, 935
Bst4CI	ACNGT	4 cut(s)	125, 1394, 1505, 1757
Bst6I	CTCTTC	2 cut(s)	229, 625
BstACI	GRCGYC	1 cut(s)	36
BstBAI	YACGTR	1 cut(s)	1346
BstC8I	GCNNGC	3 cut(s)	73, 116, 1520
BstF5I	GGATG	6 cut(s)	159, 371, 881, 1324, 1447, 1858
BstFNI	CGCG	1 cut(s)	50
BstH2I	RGCGCY	2 cut(s)	362, 1486
BstHHI	GCGC	3 cut(s)	361, 1485, 1677
BstKTI	GATC	8 cut(s)	25, 718, 730, 1032, 1298, 1384, 1411, 1834
BstMAI	GTCTC	4 cut(s)	184, 607, 608, 861
BstMBI	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
BstMCI	CGRYCG	1 cut(s)	338
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 72, 111, 214, 367, 494, 1489, 1924
BstNI	CCWGG	2 cut(s)	635, 935
BstNSI	RCATGY	2 cut(s)	470, 1522
BstSCI	CCNGG	3 cut(s)	633, 933, 1906
BstSFI	CTRYAG	2 cut(s)	393, 831
BstSLI	GKGCMC	1 cut(s)	1494
BstUI	CGCG	1 cut(s)	50
BstV1I	GCAGC	2 cut(s)	42, 1879
BstX2I	RGATCY	3 cut(s)	727, 1295, 1831
BstYI	RGATCY	3 cut(s)	727, 1295, 1831
Bsu36I	CCTNAGG	1 cut(s)	1160
BsuI	GTATCC	1 cut(s)	1112
BsuRI	GGCC	6 cut(s)	258, 1105, 1166, 1229, 1263, 1492
BtsCI	GGATG	6 cut(s)	159, 371, 881, 1324, 1447, 1858
BtsI	GCAGTG	1 cut(s)	1848
BtsIMutI	CAGTG	2 cut(s)	1472, 1848
BveI	ACCTGC	1 cut(s)	1725
Cac8I	GCNNGC	3 cut(s)	73, 116, 1520
CaiI	CAGNNNCTG	1 cut(s)	1145
CciI	TCATGA	2 cut(s)	234, 712
CfoI	GCGC	3 cut(s)	361, 1485, 1677
Cfr10I	RCCGGY	1 cut(s)	1877
Cfr13I	GGNCC	5 cut(s)	1104, 1164, 1261, 1490, 1491
CseI	GACGC	3 cut(s)	39, 44, 1574
Csp6I	GTAC	6 cut(s)	250, 373, 418, 1363, 1726, 1948
CviAII	CATG	9 cut(s)	76, 119, 235, 240, 467, 713, 887, 1124, 1519
CviQI	GTAC	6 cut(s)	250, 373, 418, 1363, 1726, 1948
DpnI	GATC	8 cut(s)	24, 717, 729, 1031, 1297, 1383, 1410, 1833
DpnII	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
DraI	TTTAAA	1 cut(s)	1645
EaeI	YGGCCR	1 cut(s)	256
Eam1104I	CTCTTC	2 cut(s)	229, 625
EarI	CTCTTC	2 cut(s)	229, 625
EciI	GGCGGA	1 cut(s)	1905
Ecl136II	GAGCTC	1 cut(s)	16
Eco130I	CCWWGG	2 cut(s)	563, 1486
Eco24I	GRGCYC	6 cut(s)	18, 224, 571, 904, 992, 1494
Eco31I	GGTCTC	1 cut(s)	608
Eco53kI	GAGCTC	1 cut(s)	16
Eco57I	CTGAAG	3 cut(s)	510, 606, 1041
Eco81I	CCTNAGG	1 cut(s)	1160
Eco88I	CYCGRG	1 cut(s)	777
EcoICRI	GAGCTC	1 cut(s)	16
EcoO109I	RGGNCCY	1 cut(s)	1261
EcoRI	GAATTC	1 cut(s)	1658
EcoRII	CCWGG	2 cut(s)	633, 933
EcoT14I	CCWWGG	2 cut(s)	563, 1486
EcoT22I	ATGCAT	1 cut(s)	1129
EcoT38I	GRGCYC	6 cut(s)	18, 224, 571, 904, 992, 1494
ErhI	CCWWGG	2 cut(s)	563, 1486
Esp3I	CGTCTC	1 cut(s)	184
FaeI	CATG	9 cut(s)	79, 122, 238, 243, 470, 716, 890, 1127, 1522
FalI	AAGNNNNNCTT	4 cut(s)	432, 464, 1586, 1618
FaqI	GGGAC	2 cut(s)	905, 1402
FatI	CATG	9 cut(s)	75, 118, 234, 239, 466, 712, 886, 1123, 1518
FauI	CCCGC	2 cut(s)	86, 641
FauNDI	CATATG	1 cut(s)	1871
Fnu4HI	GCNGC	3 cut(s)	48, 56, 1893
FokI	GGATG	6 cut(s)	146, 378, 868, 1331, 1454, 1865
FriOI	GRGCYC	6 cut(s)	18, 224, 571, 904, 992, 1494
Fsp4HI	GCNGC	3 cut(s)	48, 56, 1893
FspBI	CTAG	3 cut(s)	273, 564, 1964
GlaI	GCGC	3 cut(s)	360, 1484, 1676
GluI	GCNGC	3 cut(s)	48, 56, 1893
GsaI	CCCAGC	1 cut(s)	1630
GsuI	CTGGAG	2 cut(s)	518, 956
HaeII	RGCGCY	2 cut(s)	362, 1486
HaeIII	GGCC	6 cut(s)	258, 1105, 1166, 1229, 1263, 1492
HapII	CCGG	3 cut(s)	356, 1878, 1907
HgaI	GACGC	3 cut(s)	39, 44, 1574
HhaI	GCGC	3 cut(s)	361, 1485, 1677
Hin1I	GRCGYC	1 cut(s)	36
Hin1II	CATG	9 cut(s)	79, 122, 238, 243, 470, 716, 890, 1127, 1522
Hin6I	GCGC	3 cut(s)	359, 1483, 1675
HinP1I	GCGC	3 cut(s)	359, 1483, 1675
HindIII	AAGCTT	1 cut(s)	1005
HpaII	CCGG	3 cut(s)	356, 1878, 1907
HphI	GGTGA	3 cut(s)	523, 623, 1892
Hpy166II	GTNNAC	1 cut(s)	1695
Hpy188III	TCNNGA	8 cut(s)	142, 235, 535, 659, 713, 763, 779, 1835
Hpy8I	GTNNAC	1 cut(s)	1695
Hpy99I	CGWCG	5 cut(s)	35, 38, 55, 112, 348
HpyAV	CCTTC	6 cut(s)	656, 871, 1016, 1020, 1117, 1588
HpyCH4III	ACNGT	4 cut(s)	125, 1394, 1505, 1757
HpyCH4IV	ACGT	3 cut(s)	1345, 1361, 1728
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 72, 111, 214, 367, 494, 1489, 1924
HpySE526I	ACGT	3 cut(s)	1345, 1361, 1728
Hsp92I	GRCGYC	1 cut(s)	36
Hsp92II	CATG	9 cut(s)	79, 122, 238, 243, 470, 716, 890, 1127, 1522
HspAI	GCGC	3 cut(s)	359, 1483, 1675
Kzo9I	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
LguI	GCTCTTC	1 cut(s)	229
LmnI	GCTCC	5 cut(s)	21, 84, 1480, 1573, 1774
Lsp1109I	GCAGC	2 cut(s)	42, 1879
LweI	GCATC	6 cut(s)	69, 484, 890, 1132, 1828, 1843
MaeI	CTAG	3 cut(s)	273, 564, 1964
MaeII	ACGT	3 cut(s)	1345, 1361, 1728
MaeIII	GTNAC	5 cut(s)	763, 815, 1341, 1388, 1505
MalI	GATC	8 cut(s)	24, 717, 729, 1031, 1297, 1383, 1410, 1833
MbiI	CCGCTC	1 cut(s)	1821
MboI	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
MboII	GAAGA	4 cut(s)	210, 216, 503, 612
MflI	RGATCY	3 cut(s)	727, 1295, 1831
MhlI	GDGCHC	8 cut(s)	18, 224, 571, 904, 992, 1083, 1494, 1716
MlyI	GAGTC	3 cut(s)	444, 1031, 1454
MmeI	TCCRAC	2 cut(s)	54, 726
Mph1103I	ATGCAT	1 cut(s)	1129
MroXI	GAANNNNTTC	1 cut(s)	1610
MseI	TTAA	5 cut(s)	213, 323, 1170, 1358, 1644
MslI	CAYNNNNRTG	1 cut(s)	123
MspA1I	CMGCKG	2 cut(s)	488, 1626
MspI	CCGG	3 cut(s)	356, 1878, 1907
MspR9I	CCNGG	3 cut(s)	635, 935, 1908
MvaI	CCWGG	2 cut(s)	635, 935
MvnI	CGCG	1 cut(s)	50
MwoI	GCNNNNNNNGC	8 cut(s)	44, 72, 111, 214, 367, 494, 1489, 1924
NciI	CCSGG	1 cut(s)	1908
NdeI	CATATG	1 cut(s)	1871
NdeII	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
NlaIII	CATG	9 cut(s)	79, 122, 238, 243, 470, 716, 890, 1127, 1522
NlaIV	GGNNCC	2 cut(s)	127, 1492
NmeAIII	GCCGAG	2 cut(s)	47, 154
NmuCI	GTSAC	2 cut(s)	763, 1505
NsiI	ATGCAT	1 cut(s)	1129
NspI	RCATGY	2 cut(s)	470, 1522
PaeI	GCATGC	1 cut(s)	1522
PaeR7I	CTCGAG	1 cut(s)	777
PagI	TCATGA	2 cut(s)	234, 712
PaqCI	CACCTGC	1 cut(s)	1725
PciI	ACATGT	1 cut(s)	466
PciSI	GCTCTTC	1 cut(s)	229
PcsI	WCGNNNNNNNCGW	1 cut(s)	27
PdmI	GAANNNNTTC	1 cut(s)	1610
PfeI	GAWTC	8 cut(s)	244, 340, 890, 929, 950, 1016, 1336, 1451
PflFI	GACNNNGTC	1 cut(s)	152
PkrI	GCNGC	3 cut(s)	49, 57, 1894
PleI	GAGTC	3 cut(s)	443, 1031, 1453
PpsI	GAGTC	3 cut(s)	443, 1031, 1453
Ppu21I	YACGTR	1 cut(s)	1346
PscI	ACATGT	1 cut(s)	466
Psp124BI	GAGCTC	1 cut(s)	18
Psp6I	CCWGG	2 cut(s)	633, 933
PspFI	CCCAGC	1 cut(s)	1626
PspGI	CCWGG	2 cut(s)	633, 933
PspN4I	GGNNCC	2 cut(s)	127, 1492
PspOMI	GGGCCC	1 cut(s)	1490
PspPI	GGNCC	5 cut(s)	1104, 1164, 1261, 1490, 1491
PstNI	CAGNNNCTG	1 cut(s)	1145
PsuI	RGATCY	3 cut(s)	727, 1295, 1831
PsyI	GACNNNGTC	1 cut(s)	152
PvuII	CAGCTG	1 cut(s)	488
RsaI	GTAC	6 cut(s)	251, 374, 419, 1364, 1727, 1949
RsaNI	GTAC	6 cut(s)	250, 373, 418, 1363, 1726, 1948
RseI	CAYNNNNRTG	1 cut(s)	123
SacI	GAGCTC	1 cut(s)	18
SapI	GCTCTTC	1 cut(s)	229
SaqAI	TTAA	5 cut(s)	213, 323, 1170, 1358, 1644
SatI	GCNGC	3 cut(s)	48, 56, 1893
Sau3AI	GATC	8 cut(s)	22, 715, 727, 1029, 1295, 1381, 1408, 1831
Sau96I	GGNCC	5 cut(s)	1104, 1164, 1261, 1490, 1491
SchI	GAGTC	3 cut(s)	444, 1031, 1454
ScrFI	CCNGG	3 cut(s)	635, 935, 1908
SduI	GDGCHC	8 cut(s)	18, 224, 571, 904, 992, 1083, 1494, 1716
SfaNI	GCATC	6 cut(s)	69, 484, 890, 1132, 1828, 1843
SfcI	CTRYAG	2 cut(s)	393, 831
Sfr274I	CTCGAG	1 cut(s)	777
SlaI	CTCGAG	1 cut(s)	777
SmiMI	CAYNNNNRTG	1 cut(s)	123
SmlI	CTYRAG	1 cut(s)	777
SmoI	CTYRAG	1 cut(s)	777
SphI	GCATGC	1 cut(s)	1522
SsiI	CCGC	8 cut(s)	48, 82, 93, 334, 648, 1624, 1821, 1916
SspI	AATATT	2 cut(s)	1062, 1425
SspMI	CTAG	3 cut(s)	273, 564, 1964
SstI	GAGCTC	1 cut(s)	18
StyD4I	CCNGG	3 cut(s)	633, 933, 1906
StyI	CCWWGG	2 cut(s)	563, 1486
TaaI	ACNGT	4 cut(s)	125, 1394, 1505, 1757
TaiI	ACGT	3 cut(s)	1348, 1364, 1731
TaqI	TCGA	8 cut(s)	110, 266, 338, 778, 1035, 1380, 1662, 1788
TatI	WGTACW	2 cut(s)	372, 417
TauI	GCSGC	1 cut(s)	50
TfiI	GAWTC	8 cut(s)	244, 340, 890, 929, 950, 1016, 1336, 1451
Tru1I	TTAA	5 cut(s)	213, 323, 1170, 1358, 1644
Tru9I	TTAA	5 cut(s)	213, 323, 1170, 1358, 1644
TscAI	CASTG	2 cut(s)	1479, 1848
TseFI	GTSAC	2 cut(s)	763, 1505
TseI	GCWGC	2 cut(s)	55, 1892
Tsp45I	GTSAC	2 cut(s)	763, 1505
TspDTI	ATGAA	6 cut(s)	223, 642, 701, 1121, 1795, 1827
TspGWI	ACGGA	6 cut(s)	237, 791, 834, 1105, 1560, 1695
TspRI	CASTG	2 cut(s)	1479, 1848
Tth111I	GACNNNGTC	1 cut(s)	152
XapI	RAATTY	5 cut(s)	770, 1181, 1244, 1652, 1658
XceI	RCATGY	2 cut(s)	470, 1522
XhoI	CTCGAG	1 cut(s)	777
XmaJI	CCTAGG	1 cut(s)	563
XmnI	GAANNNNTTC	1 cut(s)	1610
XspI	CTAG	3 cut(s)	273, 564, 1964
Zsp2I	ATGCAT	1 cut(s)	1129

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.