Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0004943

Physical Location & Seq

Genomic Coordinates Forward (+)

2232593 .. 2238308

Gene Structure 5716 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G002560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1983 bp

ATGCAGGAACCGGAGCTTCATGACCTCTCCGACGACGCCGATTACGCCGCGTCGCAGCAATACGGCTCGGCGAGCATGATGCGAACCGATAGCGGCAAACACAGCTCTTCGAGCGAGCACGAAGGCGCCGAGGTCGTTTATACCAAGGATAATGTGACCATCCACCCCTCCATGCTCGCGTCGGAGAGGATTAGCGGACGATTGAGGCTATTCAAGCAAGGCTCTTCTCTGTTTATGACGTGGATTCCGTACAAGGGCCAAAACTCGAACGCTAGGGTTTCTGAGAAAGATAGAATTCTTTATACTATAAGGGGGGTGCCGTTGACAGAGGTTCGGTCCATTCGGCGCCACACTCCTGCACTTGGATGGCAGTACATTATTGTCGTTTTGGCAACTGGACTTGCGTTTCCCCCTCTGTACTTCTACAATGGAGGAGTCAAGGAGTTCCTTGCTACAATAAAGCAGCATGTGTTTATAGTGAGGTCAGCTGAGGATGCTAATGTATTTCTTGTGAATGATTTTCAAAATCCTCTGCAGAAAACTCTTTCATCTTTAGAGATACCAAGGGCTCTTTCTATTGGAAGTGAACCATCTACATCATTCTCAGTTGGCGAGTCCTCTGTAGATGAAAATCAGGAGAGAACAAGTGAGGATGTTCAGAATGATAATACAACAATTTCTCAATATAATGAGAGGCAAAGGCAAAAGAATTATGATCCTGCACGAGATCTCTCAATTCAAGTGTTGGAAAAATTCTCTCTGGTGACAAAATTTGCTCGAGAAACAACTTCGCAACTCTTCCGTGATGGCCATAGTAATGGATATGGTAGTATAGGAAGGAGAAGTAATAGCCTGTCCTCCCTTGATTACCCCTCTCAAAAGCCATCCGATGATTCGAACAAAGTCTCTGATAAAAGTCCGGTCCCCCAAGATCCACTGGAGTTTGACAAGAATTCACTGGTATGGGGAAAACCACGACAGCCACCTTTGGAGTCTGCTGAGTGGTACACCTTCTTGGATTCTGAAGGGCGGATCATGGATTCAGAAGCTTTAAGAAAGAGAATATTTTATGGGGGAGTTGAGCACAAATTACGGAAGGAGGTCTGGCCATTCTTGCTGGGATACCATGCGTATGATTCAACATATGCAGAAAGGGAGTATCTTAAGGCAATGAAGAAGGCAGAGTTTGAGACAGTCAAGAAGCAGTGGCAGAGTATCTCCCCAGAGCAGGCAAGAAGGTTTACAAAATTCAGGGAGAGAAAAGGCCTTATTGACAAAGATGTGGTGAGGACCGACAGATCTATTTCCTTCTATGATGGGGATGATAATCCAAATGTGAATCTGTTGCATGATATATTGCTAACATACTCGTTTTACAACTTTGATCTTGGTTACTGTCAGGGTATGAGTGATCTTCTGTCCCCAATATTGTTTGTACTGGGGGATGAATCAGAATCATTTTGGTGTTTTGTTAAACTGATGGAGCGCCTTGGGCCCAATTTTAACCGTGACCAAAATGGCATGCACTCTCAACTTTTTGCCATAACTAAGTTGGTGGAGATGTTGGACAGCCCATTGCATAATTACTTTACGCAGCGTGACTGCTTGAATTACTTCTTTTGTTTCCGCTGGGTTCTGATACAGTTTAAAAGGGAATTTGAATTCGACAAGACATTGAGATTATGGGAGGTACTGTGGACACATTATCCATCTGAGCACCTGCACCTGTATGTGTGTGTTGCTATACTCAAACGATACCGTAGTAAGATAATTGGGGAGCAGATGGACTTTGACACTCTACTGAAGTTCATCAATGAACTGAGTGGTCAAATAAATCTTGATGCCGTTGTGAGGGATGCAGAAGCTCTGTGCATATGTGCTGGTGAGAACGGTGATGCTTGCATTCCACCTGGAACTCCACCCTCACTGCCTGTTGACGATGGTTTGCTGTACCCTCAACAAGAACTAGATGAGATATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006886 GO:0008047 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015833 GO:0017016 GO:0017137 GO:0019899 GO:0030234 GO:0030695 GO:0031267 GO:0031338 GO:0032879 GO:0033036 GO:0033043 GO:0034613 GO:0042886 GO:0043085 GO:0043087 GO:0043547 GO:0044093 GO:0044424 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0060589 GO:0060627 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071705 GO:0090630 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

DUF3548 RabGAP-TBC

Protein Analysis

660

Amino Acids

75.89

Weight (kDa)

5.48

Isoelectric Point (pI)

46.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_RBD	PF12068	45 - 186	1.7e-18	Rab-binding domain (RBD)
RabGAP-TBC	PF00566	363 - 591	6.6e-49	Rab-GTPase-TBC domain
RabGap-TBC_2	PF23436	449 - 606	3.6e-16	RabGAP TBC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52580 AT5G52580 AT5G52580
fragaria_vesca	FvH4_1g02680
malus_domestica	MD02G1027400.v1.1 MD15G1168800.v1.1
prunus_persica	Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1
pyrus_communis	pycom02g02230 pycom15g15050
rosa_chinensis	RchiOBHm_Chr2g0087771 RchiOBHm_Chr7g0194011
rosa_laevigata	RLG00000004223 RLG00000015897
rosa_multiflora	Rmu_sc0021871.1_g000005
rosa_roxburghii	Rroxscaffold_2G00153250 Rroxscaffold_3G00261620
rosa_rugosa	Rorug01G0478700 Rorug07G0013200
rosa_samantha	Rh2AG032900 Rh2CG032700 Rh2DG032500 Rh7AG140400 Rh7BG140900 Rh7CG144400 Rh7DG141700
rosa_wichuraiana	Rw2G002560 Rw7G011980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1728
Acc36I	ACCTGC	1 cut(s)	1728
AccB1I	GGYRCC	3 cut(s)	125, 316, 345
AccII	CGCG	2 cut(s)	50, 179
AciI	CCGC	5 cut(s)	48, 93, 195, 1030, 1627
AclWI	GGATC	3 cut(s)	710, 926, 1040
AcoI	YGGCCR	2 cut(s)	808, 1106
AcsI	RAATTY	7 cut(s)	294, 752, 770, 952, 1247, 1655, 1661
AcuI	CTGAAG	2 cut(s)	1044, 1823
AcyI	GRCGYC	3 cut(s)	36, 126, 346
AfaI	GTAC	7 cut(s)	251, 374, 419, 1007, 1437, 1692, 1952
AfiI	CCNNNNNNNGG	4 cut(s)	254, 362, 1228, 1851
AflII	CTTAAG	1 cut(s)	1163
AgsI	TTSAA	6 cut(s)	214, 524, 740, 1140, 1609, 1661
AjiI	CACGTC	1 cut(s)	240
AjnI	CCWGG	1 cut(s)	1909
AjuI	GAANNNNNNNTTGG	2 cut(s)	556, 588
AluBI	AGCT	5 cut(s)	16, 105, 488, 1049, 1865
AluI	AGCT	5 cut(s)	16, 105, 488, 1049, 1865
Alw21I	GWGCWC	3 cut(s)	120, 1086, 1719
Alw26I	GTCTC	2 cut(s)	910, 1184
AlwI	GGATC	3 cut(s)	710, 926, 1040
Ama87I	CYCGRG	1 cut(s)	777
AoxI	GGCC	5 cut(s)	256, 808, 1106, 1264, 1493
ApaI	GGGCCC	1 cut(s)	1497
ApeKI	GCWGC	3 cut(s)	55, 463, 1594
ApoI	RAATTY	7 cut(s)	294, 752, 770, 952, 1247, 1655, 1661
Asp700I	GAANNNNTTC	1 cut(s)	1613
AspLEI	GCGC	3 cut(s)	128, 348, 1488
AspS9I	GGNCC	6 cut(s)	256, 336, 922, 1290, 1493, 1494
AsuHPI	GGTGA	4 cut(s)	775, 1297, 1895, 1904
AsuII	TTCGAA	1 cut(s)	896
AvaI	CYCGRG	1 cut(s)	777
AvaII	GGWCC	3 cut(s)	336, 922, 1290
BaeGI	GKGCMC	1 cut(s)	1497
BaeI	ACNNNNGTAYC	2 cut(s)	1747, 1780
BalI	TGGCCA	2 cut(s)	810, 1108
BanI	GGYRCC	3 cut(s)	125, 316, 345
BanII	GRGCYC	2 cut(s)	571, 1497
BauI	CACGAG	1 cut(s)	723
Bbv12I	GWGCWC	3 cut(s)	120, 1086, 1719
BbvCI	CCTCAGC	1 cut(s)	489
BbvI	GCAGC	3 cut(s)	67, 475, 1606
BceAI	ACGGC	3 cut(s)	79, 304, 1829
BcgI	CGANNNNNNTGC	2 cut(s)	61, 95
BciT130I	CCWGG	1 cut(s)	1911
BciVI	GTATCC	1 cut(s)	1115
BcoDI	GTCTC	2 cut(s)	910, 1184
BfaI	CTAG	2 cut(s)	273, 1967
BfmI	CTRYAG	2 cut(s)	533, 621
BfoI	RGCGCY	3 cut(s)	129, 349, 1489
BfrI	CTTAAG	1 cut(s)	1163
BfuAI	ACCTGC	1 cut(s)	1728
BfuI	GTATCC	1 cut(s)	1115
BglII	AGATCT	2 cut(s)	727, 1298
BisI	GCNGC	5 cut(s)	48, 56, 94, 464, 1595
BlsI	GCNGC	5 cut(s)	49, 57, 95, 465, 1596
Bme1390I	CCNGG	1 cut(s)	1911
Bme18I	GGWCC	3 cut(s)	336, 922, 1290
BmeT110I	CYCGRG	1 cut(s)	777
BmgBI	CACGTC	1 cut(s)	240
BmgT120I	GGNCC	6 cut(s)	256, 336, 922, 1290, 1493, 1494
BmiI	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1495
BmrFI	CCNGG	1 cut(s)	1911
BmrI	ACTGGG	1 cut(s)	1448
BmsI	GCATC	5 cut(s)	69, 484, 1831, 1846, 1885
BmuI	ACTGGG	1 cut(s)	1448
BpmI	CTGGAG	1 cut(s)	959
Bpu10I	CCTNAGC	1 cut(s)	489
Bpu14I	TTCGAA	1 cut(s)	896
BsaBI	GATNNNNATC	2 cut(s)	630, 1326
BsaHI	GRCGYC	3 cut(s)	36, 126, 346
BsaJI	CCNNGG	4 cut(s)	129, 144, 563, 1489
BsaWI	WCCGGW	2 cut(s)	10, 919
BsaXI	ACNNNNNCTCC	2 cut(s)	1679, 1709
Bsc4I	CCNNNNNNNGG	4 cut(s)	254, 362, 1228, 1851
Bse1I	ACTGG	4 cut(s)	400, 942, 963, 1443
Bse3DI	GCAATG	2 cut(s)	1176, 1574
Bse8I	GATNNNNATC	2 cut(s)	630, 1326
BseBI	CCWGG	1 cut(s)	1911
BseDI	CCNNGG	4 cut(s)	129, 144, 563, 1489
BseGI	GGATG	8 cut(s)	159, 371, 499, 658, 884, 1327, 1450, 1861
BseJI	GATNNNNATC	2 cut(s)	630, 1326
BseLI	CCNNNNNNNGG	4 cut(s)	254, 362, 1228, 1851
BseMI	GCAATG	2 cut(s)	1176, 1574
BseMII	CTCAG	6 cut(s)	273, 480, 618, 990, 1704, 1811
BseNI	ACTGG	4 cut(s)	400, 942, 963, 1443
BseRI	GAGGAG	1 cut(s)	447
BseSI	GKGCMC	1 cut(s)	1497
BseXI	GCAGC	3 cut(s)	67, 475, 1606
BseYI	CCCAGC	2 cut(s)	1117, 1629
BsgI	GTGCAG	3 cut(s)	342, 705, 1706
Bsh1236I	CGCG	2 cut(s)	50, 179
BshFI	GGCC	5 cut(s)	258, 810, 1108, 1266, 1495
BshNI	GGYRCC	3 cut(s)	125, 316, 345
BsiHKAI	GWGCWC	3 cut(s)	120, 1086, 1719
BsiHKCI	CYCGRG	1 cut(s)	777
BsiSI	CCGG	2 cut(s)	11, 920
BslFI	GGGAC	2 cut(s)	908, 1405
BslI	CCNNNNNNNGG	4 cut(s)	254, 362, 1228, 1851
BsmAI	GTCTC	2 cut(s)	910, 1184
BsmFI	GGGAC	2 cut(s)	908, 1405
BsmI	GAATGC	1 cut(s)	1902
BsnI	GGCC	5 cut(s)	258, 810, 1108, 1266, 1495
BsoBI	CYCGRG	1 cut(s)	777
Bsp119I	TTCGAA	1 cut(s)	896
Bsp120I	GGGCCC	1 cut(s)	1493
Bsp1286I	GDGCHC	5 cut(s)	120, 571, 1086, 1497, 1719
Bsp143I	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
BspACI	CCGC	5 cut(s)	48, 93, 195, 1030, 1627
BspANI	GGCC	5 cut(s)	258, 810, 1108, 1266, 1495
BspCNI	CTCAG	6 cut(s)	274, 481, 617, 991, 1705, 1812
BspFNI	CGCG	2 cut(s)	50, 179
BspHI	TCATGA	1 cut(s)	19
BspLI	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1495
BspMAI	CTGCAG	1 cut(s)	537
BspMI	ACCTGC	1 cut(s)	1728
BspPI	GGATC	3 cut(s)	710, 926, 1040
BspQI	GCTCTTC	2 cut(s)	112, 229
BspT104I	TTCGAA	1 cut(s)	896
BspT107I	GGYRCC	3 cut(s)	125, 316, 345
BspTI	CTTAAG	1 cut(s)	1163
BsrDI	GCAATG	2 cut(s)	1176, 1574
BsrI	ACTGG	4 cut(s)	400, 942, 963, 1443
BssECI	CCNNGG	4 cut(s)	129, 144, 563, 1489
BssMI	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
BssNI	GRCGYC	3 cut(s)	36, 126, 346
BssSI	CACGAG	1 cut(s)	723
BssT1I	CCWWGG	3 cut(s)	144, 563, 1489
Bst2BI	CACGAG	1 cut(s)	723
Bst2UI	CCWGG	1 cut(s)	1911
Bst4CI	ACNGT	7 cut(s)	1195, 1397, 1508, 1644, 1695, 1760, 1892
Bst6I	CTCTTC	3 cut(s)	112, 229, 803
BstACI	GRCGYC	3 cut(s)	36, 126, 346
BstAFI	CTTAAG	1 cut(s)	1163
BstBI	TTCGAA	1 cut(s)	896
BstC8I	GCNNGC	6 cut(s)	73, 116, 177, 1230, 1523, 1900
BstDEI	CTNAG	7 cut(s)	282, 489, 604, 999, 1548, 1713, 1820
BstF5I	GGATG	8 cut(s)	159, 371, 499, 658, 884, 1327, 1450, 1861
BstFNI	CGCG	2 cut(s)	50, 179
BstH2I	RGCGCY	3 cut(s)	129, 349, 1489
BstHHI	GCGC	3 cut(s)	128, 348, 1488
BstKTI	GATC	7 cut(s)	718, 730, 934, 1035, 1301, 1387, 1414
BstMAI	GTCTC	2 cut(s)	910, 1184
BstMBI	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 72, 102, 111, 214, 494, 1114, 1492
BstNI	CCWGG	1 cut(s)	1911
BstNSI	RCATGY	2 cut(s)	470, 1525
BstSCI	CCNGG	1 cut(s)	1909
BstSFI	CTRYAG	2 cut(s)	533, 621
BstSLI	GKGCMC	1 cut(s)	1497
BstUI	CGCG	2 cut(s)	50, 179
BstV1I	GCAGC	3 cut(s)	67, 475, 1606
BstX2I	RGATCY	3 cut(s)	727, 931, 1298
BstXI	CCANNNNNNTGG	1 cut(s)	818
BstYI	RGATCY	3 cut(s)	727, 931, 1298
BsuI	GTATCC	1 cut(s)	1115
BsuRI	GGCC	5 cut(s)	258, 810, 1108, 1266, 1495
BtrI	CACGTC	1 cut(s)	240
BtsCI	GGATG	8 cut(s)	159, 371, 499, 658, 884, 1327, 1450, 1861
BtsI	GCAGTG	2 cut(s)	1211, 1925
BtsIMutI	CAGTG	4 cut(s)	935, 956, 1211, 1925
BveI	ACCTGC	1 cut(s)	1728
Cac8I	GCNNGC	6 cut(s)	73, 116, 177, 1230, 1523, 1900
CciI	TCATGA	1 cut(s)	19
CfoI	GCGC	3 cut(s)	128, 348, 1488
Cfr13I	GGNCC	6 cut(s)	256, 336, 922, 1290, 1493, 1494
CseI	GACGC	3 cut(s)	39, 44, 168
Csp6I	GTAC	7 cut(s)	250, 373, 418, 1006, 1436, 1691, 1951
CviAII	CATG	8 cut(s)	20, 76, 172, 467, 1036, 1127, 1349, 1522
CviQI	GTAC	7 cut(s)	250, 373, 418, 1006, 1436, 1691, 1951
DdeI	CTNAG	7 cut(s)	282, 489, 604, 999, 1548, 1713, 1820
DinI	GGCGCC	2 cut(s)	127, 347
DpnI	GATC	7 cut(s)	717, 729, 933, 1034, 1300, 1386, 1413
DpnII	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
DraI	TTTAAA	1 cut(s)	1648
EaeI	YGGCCR	2 cut(s)	808, 1106
Eam1104I	CTCTTC	3 cut(s)	112, 229, 803
EarI	CTCTTC	3 cut(s)	112, 229, 803
EciI	GGCGGA	1 cut(s)	1045
Eco130I	CCWWGG	3 cut(s)	144, 563, 1489
Eco147I	AGGCCT	1 cut(s)	1266
Eco24I	GRGCYC	2 cut(s)	571, 1497
Eco47I	GGWCC	3 cut(s)	336, 922, 1290
Eco57I	CTGAAG	2 cut(s)	1044, 1823
Eco88I	CYCGRG	1 cut(s)	777
EcoRI	GAATTC	3 cut(s)	294, 952, 1661
EcoRII	CCWGG	1 cut(s)	1909
EcoT14I	CCWWGG	3 cut(s)	144, 563, 1489
EcoT38I	GRGCYC	2 cut(s)	571, 1497
EgeI	GGCGCC	2 cut(s)	127, 347
EheI	GGCGCC	2 cut(s)	127, 347
ErhI	CCWWGG	3 cut(s)	144, 563, 1489
FaeI	CATG	8 cut(s)	23, 79, 175, 470, 1039, 1130, 1352, 1525
FaqI	GGGAC	2 cut(s)	908, 1405
FatI	CATG	8 cut(s)	19, 75, 171, 466, 1035, 1126, 1348, 1521
FauNDI	CATATG	2 cut(s)	1144, 1874
Fnu4HI	GCNGC	5 cut(s)	48, 56, 94, 464, 1595
FokI	GGATG	8 cut(s)	146, 378, 506, 665, 871, 1334, 1457, 1868
FriOI	GRGCYC	2 cut(s)	571, 1497
Fsp4HI	GCNGC	5 cut(s)	48, 56, 94, 464, 1595
FspBI	CTAG	2 cut(s)	273, 1967
GlaI	GCGC	3 cut(s)	127, 347, 1487
GluI	GCNGC	5 cut(s)	48, 56, 94, 464, 1595
GsaI	CCCAGC	2 cut(s)	1121, 1633
GsuI	CTGGAG	1 cut(s)	959
HaeII	RGCGCY	3 cut(s)	129, 349, 1489
HaeIII	GGCC	5 cut(s)	258, 810, 1108, 1266, 1495
HapII	CCGG	2 cut(s)	11, 920
HgaI	GACGC	3 cut(s)	39, 44, 168
HhaI	GCGC	3 cut(s)	128, 348, 1488
Hin1I	GRCGYC	3 cut(s)	36, 126, 346
Hin1II	CATG	8 cut(s)	23, 79, 175, 470, 1039, 1130, 1352, 1525
Hin6I	GCGC	3 cut(s)	126, 346, 1486
HinP1I	GCGC	3 cut(s)	126, 346, 1486
HincII	GTYRAC	2 cut(s)	324, 1936
HindII	GTYRAC	2 cut(s)	324, 1936
HindIII	AAGCTT	1 cut(s)	1047
HpaII	CCGG	2 cut(s)	11, 920
HphI	GGTGA	4 cut(s)	775, 1297, 1895, 1904
Hpy166II	GTNNAC	6 cut(s)	324, 587, 1008, 1242, 1698, 1936
Hpy188III	TCNNGA	5 cut(s)	20, 635, 779, 1198, 1838
Hpy8I	GTNNAC	6 cut(s)	324, 587, 1008, 1242, 1698, 1936
Hpy99I	CGWCG	4 cut(s)	35, 38, 55, 184
HpyAV	CCTTC	8 cut(s)	116, 831, 1019, 1021, 1090, 1171, 1230, 1318
HpyCH4III	ACNGT	7 cut(s)	1195, 1397, 1508, 1644, 1695, 1760, 1892
HpyCH4IV	ACGT	1 cut(s)	239
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 72, 102, 111, 214, 494, 1114, 1492
HpyF3I	CTNAG	7 cut(s)	282, 489, 604, 999, 1548, 1713, 1820
HpySE526I	ACGT	1 cut(s)	239
Hsp92I	GRCGYC	3 cut(s)	36, 126, 346
Hsp92II	CATG	8 cut(s)	23, 79, 175, 470, 1039, 1130, 1352, 1525
HspAI	GCGC	3 cut(s)	126, 346, 1486
KasI	GGCGCC	2 cut(s)	125, 345
Kzo9I	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
LguI	GCTCTTC	2 cut(s)	112, 229
LmnI	GCTCC	3 cut(s)	13, 1483, 1777
Lsp1109I	GCAGC	3 cut(s)	67, 475, 1606
LweI	GCATC	5 cut(s)	69, 484, 1831, 1846, 1885
MaeI	CTAG	2 cut(s)	273, 1967
MaeII	ACGT	1 cut(s)	239
MaeIII	GTNAC	5 cut(s)	154, 763, 1391, 1508, 1598
MalI	GATC	7 cut(s)	717, 729, 933, 1034, 1300, 1386, 1413
MboI	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
MboII	GAAGA	5 cut(s)	99, 216, 790, 1186, 1406
MflI	RGATCY	3 cut(s)	727, 931, 1298
MhlI	GDGCHC	5 cut(s)	120, 571, 1086, 1497, 1719
MlsI	TGGCCA	2 cut(s)	810, 1108
MluNI	TGGCCA	2 cut(s)	810, 1108
Mly113I	GGCGCC	2 cut(s)	126, 346
MlyI	GAGTC	3 cut(s)	444, 623, 1001
MmeI	TCCRAC	4 cut(s)	54, 162, 726, 1545
Mox20I	TGGCCA	2 cut(s)	810, 1108
MroXI	GAANNNNTTC	1 cut(s)	1613
MscI	TGGCCA	2 cut(s)	810, 1108
MseI	TTAA	5 cut(s)	1052, 1164, 1473, 1503, 1647
MslI	CAYNNNNRTG	6 cut(s)	364, 816, 961, 1131, 1462, 1728
Msp20I	TGGCCA	2 cut(s)	810, 1108
MspA1I	CMGCKG	2 cut(s)	488, 1629
MspCI	CTTAAG	1 cut(s)	1163
MspI	CCGG	2 cut(s)	11, 920
MspR9I	CCNGG	1 cut(s)	1911
Mva1269I	GAATGC	1 cut(s)	1902
MvaI	CCWGG	1 cut(s)	1911
MvnI	CGCG	2 cut(s)	50, 179
MwoI	GCNNNNNNNGC	8 cut(s)	44, 72, 102, 111, 214, 494, 1114, 1492
NarI	GGCGCC	2 cut(s)	126, 346
NdeI	CATATG	2 cut(s)	1144, 1874
NdeII	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
NlaIII	CATG	8 cut(s)	23, 79, 175, 470, 1039, 1130, 1352, 1525
NlaIV	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1495
NmeAIII	GCCGAG	2 cut(s)	47, 154
NmuCI	GTSAC	4 cut(s)	154, 763, 1508, 1598
NspI	RCATGY	2 cut(s)	470, 1525
NspV	TTCGAA	1 cut(s)	896
PaeI	GCATGC	1 cut(s)	1525
PaeR7I	CTCGAG	1 cut(s)	777
PagI	TCATGA	1 cut(s)	19
PaqCI	CACCTGC	1 cut(s)	1728
PceI	AGGCCT	1 cut(s)	1266
PciSI	GCTCTTC	2 cut(s)	112, 229
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 245
PctI	GAATGC	1 cut(s)	1902
PdmI	GAANNNNTTC	1 cut(s)	1613
PfeI	GAWTC	8 cut(s)	244, 893, 1019, 1040, 1136, 1339, 1448, 1454
PkrI	GCNGC	5 cut(s)	49, 57, 95, 465, 1596
PleI	GAGTC	3 cut(s)	443, 622, 1000
PluTI	GGCGCC	2 cut(s)	129, 349
PpsI	GAGTC	3 cut(s)	443, 622, 1000
Psp6I	CCWGG	1 cut(s)	1909
PspFI	CCCAGC	2 cut(s)	1117, 1629
PspGI	CCWGG	1 cut(s)	1909
PspN4I	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1495
PspOMI	GGGCCC	1 cut(s)	1493
PspPI	GGNCC	6 cut(s)	256, 336, 922, 1290, 1493, 1494
PstI	CTGCAG	1 cut(s)	537
PsuI	RGATCY	3 cut(s)	727, 931, 1298
PvuII	CAGCTG	1 cut(s)	488
RsaI	GTAC	7 cut(s)	251, 374, 419, 1007, 1437, 1692, 1952
RsaNI	GTAC	7 cut(s)	250, 373, 418, 1006, 1436, 1691, 1951
RseI	CAYNNNNRTG	6 cut(s)	364, 816, 961, 1131, 1462, 1728
SapI	GCTCTTC	2 cut(s)	112, 229
SaqAI	TTAA	5 cut(s)	1052, 1164, 1473, 1503, 1647
SatI	GCNGC	5 cut(s)	48, 56, 94, 464, 1595
Sau3AI	GATC	7 cut(s)	715, 727, 931, 1032, 1298, 1384, 1411
Sau96I	GGNCC	6 cut(s)	256, 336, 922, 1290, 1493, 1494
SchI	GAGTC	3 cut(s)	444, 623, 1001
ScrFI	CCNGG	1 cut(s)	1911
SduI	GDGCHC	5 cut(s)	120, 571, 1086, 1497, 1719
SfaNI	GCATC	5 cut(s)	69, 484, 1831, 1846, 1885
SfcI	CTRYAG	2 cut(s)	533, 621
SfoI	GGCGCC	2 cut(s)	127, 347
Sfr274I	CTCGAG	1 cut(s)	777
SfuI	TTCGAA	1 cut(s)	896
SinI	GGWCC	3 cut(s)	336, 922, 1290
SlaI	CTCGAG	1 cut(s)	777
SmiMI	CAYNNNNRTG	6 cut(s)	364, 816, 961, 1131, 1462, 1728
SmlI	CTYRAG	2 cut(s)	777, 1163
SmoI	CTYRAG	2 cut(s)	777, 1163
SphI	GCATGC	1 cut(s)	1525
SseBI	AGGCCT	1 cut(s)	1266
SsiI	CCGC	5 cut(s)	48, 93, 195, 1030, 1627
SspDI	GGCGCC	2 cut(s)	125, 345
SspI	AATATT	2 cut(s)	1065, 1428
SspMI	CTAG	2 cut(s)	273, 1967
StuI	AGGCCT	1 cut(s)	1266
StyD4I	CCNGG	1 cut(s)	1909
StyI	CCWWGG	3 cut(s)	144, 563, 1489
TaaI	ACNGT	7 cut(s)	1195, 1397, 1508, 1644, 1695, 1760, 1892
TaiI	ACGT	1 cut(s)	242
TaqI	TCGA	5 cut(s)	110, 266, 778, 896, 1665
TaqII	GACCGA	2 cut(s)	324, 1307
TatI	WGTACW	3 cut(s)	372, 417, 1435
TauI	GCSGC	2 cut(s)	50, 96
TfiI	GAWTC	8 cut(s)	244, 893, 1019, 1040, 1136, 1339, 1448, 1454
Tru1I	TTAA	5 cut(s)	1052, 1164, 1473, 1503, 1647
Tru9I	TTAA	5 cut(s)	1052, 1164, 1473, 1503, 1647
TscAI	CASTG	4 cut(s)	942, 963, 1211, 1932
TseFI	GTSAC	4 cut(s)	154, 763, 1508, 1598
TseI	GCWGC	3 cut(s)	55, 463, 1594
Tsp45I	GTSAC	4 cut(s)	154, 763, 1508, 1598
TspDTI	ATGAA	7 cut(s)	8, 537, 642, 1187, 1461, 1798, 1830
TspGWI	ACGGA	3 cut(s)	237, 791, 1108
TspRI	CASTG	4 cut(s)	942, 963, 1211, 1932
Vha464I	CTTAAG	1 cut(s)	1163
VpaK11BI	GGWCC	3 cut(s)	336, 922, 1290
XapI	RAATTY	7 cut(s)	294, 752, 770, 952, 1247, 1655, 1661
XceI	RCATGY	2 cut(s)	470, 1525
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1549
XhoI	CTCGAG	1 cut(s)	777
XmnI	GAANNNNTTC	1 cut(s)	1613
XspI	CTAG	2 cut(s)	273, 1967

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.