Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0004943

Physical Location & Seq

Genomic Coordinates Forward (+)

2289433 .. 2295185

Gene Structure 5753 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015897

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGCAGGAACCGGAGCTCCACGACCTCTCCGACGACGCCGATTACGCAGCGTCGCAGCAATACGGCTCGGCGAGCATGATGCGAACCGATAGCGGCAAACACAGCTCTTCGAGCGAGCATGAAGGCGCCGAGGTCGTTTATACCAAGGATAATGTGACCATCCACCCCTCCATGCTCGCGTCGGAGAGGATTAGCGGACGATTGAGGCTAATCAAGCAAGGCTCTTCTCTGTTTATGACGTGGATTCCATACAAGGGGCAAAACTCCAACGCTAGGGTTTCTGAGAAAGATAGAATTCTTTATACTATAAGGGGGGTGCCGTTGACAGAGGTTCGGTCCATTCGGCGCCACACTCCTGCACTTGGATGGCAGTACATTATCGTTGTTTTGGCAACTGGACTTGCATTTCCCCCTCTGTACTTCTACAATGGAGGAGTCAAGGAGTTCCTTGCTACAATAAAGCAGCATGTGTTTATAGTGAGGTCAGCTGAGGATGCTAATGTATTTCTTGTGAATGATTTTCAAAATCCTCTGCAGAAAACTCTTTCATCTTTAGAGATACCAAGGGCTCTTTCTATTGGAAGTGAACCATCTACATCATTCTCAGTTGGCGAGTCCTCTGTAGATGAAAATCAGGAGAGAACAAGTGAGGATGTTCAGAATGATAATACAACAATTTCTCAATATAATGAGAGGCAAAGGCAAAAGAATTATGATCCTGCACGAGATCTCTCAATTCAAGTGTTGGAAAAATTCTCTCTGGTGACAAAATTTGCTCGAGAAACAACTTCACAACTCTTCCGTGATGGCCATAGTAATGGATATGGTAGTATAGGAAGGAGAAGTAATAGCTTGTCTTCCCTTGATTACCCCTCTCAAAAGGCATCCGATGATTCGAACAAAGTCTCTGATAAAAGTCCGGTCCCCCAAGATCCACTGGAGAATTCACTGGTATGGGGAAAACCACGACAGCCACCTTTGGAGTCTGCTGAGTGGTACACCTTCTTGGATTCTGAAGGGCGGATCATGGATTCAGAAGCTTTAAGAAAGAGAATATTTTATGGGGGAGTTGAGCACAAATTACGGAAGGAGGTCTGGCCATTCTTGCTGGGATACCATGCATATGATTCAACATATGCAGAAAGGGAGTATCTTAAGGCAATGAAGAAGGCAGAGTTTGAGACAGTCAAGAAGCAGTGGCAGAGTATCTCCCCAGAGCAGGCAAGAAGGTTTACAAAATTCAGGGAGAGAAAAGGCCTTATTGACAAAGATGTGGTGAGGACCGACAGATCTATTTCCTTCTACGATGGGGATGATAATCCAAATGTGAATCTGTTGCATGATATATTGCTAACATACTCGTTTTACAACTTCGATCTTGGTTACTGTCAGGGTATGAGTGATCTTCTGTCCCCAATATTGTTTGTACTGGGGGATGAGTCAGAATCATTTTGGTGTTTTGTTAAACTGATGGAGCGCCTTGGGCCCAATTTTAACCGTGACCAAAATGGCATGCACTCTCAACTTTTTGCCATAACTAAGTTGGTGGAGATGTTGGACAGCCCATTGCATAATTACTTTACGCAGCGTGACTGCTTGAATTACTTCTTTTGTTTCCGCTGGGTTCTGATACAGTTTAAAAGGGAATTTGAATTCGACAAGACATTGAGATTATGGGAGGTACTGTGGACACATTATCCATCTGAGCACCTGCACCTGTATGTGTGTGTTGCTATACTCAAACGATATGGTAGTAAGATAATTGGGGAGCAGATGGACTTCGACACTCTACTGAAGTTCATCAATGAACTGAGTGGTCAAATAAATCTTGATGCCGTTGTGAGGGATGCAGAAGCTCTGTGCATATGTGCTGGTGAGAACGGTGATGCTTGCATTCCACCTGGAACTCCACCCTCACTGCCTGTTGACGATGGTCTGCTGTACCCTCAACAAGAACTAGATGAGATATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006886 GO:0008047 GO:0008104 GO:0008150 GO:0012505 GO:0015031 GO:0015833 GO:0017016 GO:0017137 GO:0019899 GO:0030234 GO:0030695 GO:0031267 GO:0031338 GO:0032879 GO:0033036 GO:0033043 GO:0034613 GO:0042886 GO:0043085 GO:0043087 GO:0043547 GO:0044093 GO:0044424 GO:0044464 GO:0045184 GO:0046907 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0060589 GO:0060627 GO:0065007 GO:0065009 GO:0070727 GO:0071702 GO:0071705 GO:0090630 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

DUF3548 RabGAP-TBC

Protein Analysis

658

Amino Acids

75.34

Weight (kDa)

5.42

Isoelectric Point (pI)

45.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH_RBD	PF12068	45 - 186	1.3e-18	Rab-binding domain (RBD)
RabGAP-TBC	PF00566	360 - 587	2.2e-48	Rab-GTPase-TBC domain
RabGap-TBC_2	PF23436	446 - 603	9.4e-16	RabGAP TBC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004943)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52580 AT5G52580 AT5G52580
fragaria_vesca	FvH4_1g02680
malus_domestica	MD02G1027400.v1.1 MD15G1168800.v1.1
prunus_persica	Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1 Prupe.7G247700_v2.0.a1
pyrus_communis	pycom02g02230 pycom15g15050
rosa_chinensis	RchiOBHm_Chr2g0087771 RchiOBHm_Chr7g0194011
rosa_laevigata	RLG00000004223 RLG00000015897
rosa_multiflora	Rmu_sc0021871.1_g000005
rosa_roxburghii	Rroxscaffold_2G00153250 Rroxscaffold_3G00261620
rosa_rugosa	Rorug01G0478700 Rorug07G0013200
rosa_samantha	Rh2AG032900 Rh2CG032700 Rh2DG032500 Rh7AG140400 Rh7BG140900 Rh7CG144400 Rh7DG141700
rosa_wichuraiana	Rw2G002560 Rw7G011980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1719
Acc36I	ACCTGC	1 cut(s)	1719
AccB1I	GGYRCC	3 cut(s)	125, 316, 345
AccII	CGCG	1 cut(s)	179
AciI	CCGC	4 cut(s)	93, 195, 1021, 1618
AclWI	GGATC	3 cut(s)	710, 926, 1031
AcoI	YGGCCR	2 cut(s)	808, 1097
AcsI	RAATTY	7 cut(s)	294, 752, 770, 943, 1238, 1646, 1652
AcuI	CTGAAG	2 cut(s)	1035, 1814
AcyI	GRCGYC	3 cut(s)	36, 126, 346
AfaI	GTAC	6 cut(s)	374, 419, 998, 1428, 1683, 1943
AfiI	CCNNNNNNNGG	5 cut(s)	254, 273, 362, 1219, 1842
AflII	CTTAAG	1 cut(s)	1154
AgsI	TTSAA	5 cut(s)	524, 740, 1131, 1600, 1652
AjiI	CACGTC	1 cut(s)	240
AjnI	CCWGG	1 cut(s)	1900
AjuI	GAANNNNNNNTTGG	2 cut(s)	556, 588
AloI	GAACNNNNNNTCC	1 cut(s)	32
AluBI	AGCT	6 cut(s)	16, 105, 488, 852, 1040, 1856
AluI	AGCT	6 cut(s)	16, 105, 488, 852, 1040, 1856
Alw21I	GWGCWC	3 cut(s)	18, 1077, 1710
Alw26I	GTCTC	2 cut(s)	910, 1175
AlwI	GGATC	3 cut(s)	710, 926, 1031
Ama87I	CYCGRG	1 cut(s)	777
AoxI	GGCC	4 cut(s)	808, 1097, 1255, 1484
ApaI	GGGCCC	1 cut(s)	1488
ApeKI	GCWGC	4 cut(s)	47, 55, 463, 1585
ApoI	RAATTY	7 cut(s)	294, 752, 770, 943, 1238, 1646, 1652
Asp700I	GAANNNNTTC	1 cut(s)	1604
AspLEI	GCGC	3 cut(s)	128, 348, 1479
AspS9I	GGNCC	5 cut(s)	336, 922, 1281, 1484, 1485
AsuHPI	GGTGA	4 cut(s)	775, 1288, 1886, 1895
AsuII	TTCGAA	1 cut(s)	896
AvaI	CYCGRG	1 cut(s)	777
AvaII	GGWCC	3 cut(s)	336, 922, 1281
BaeGI	GKGCMC	1 cut(s)	1488
BalI	TGGCCA	2 cut(s)	810, 1099
BanI	GGYRCC	3 cut(s)	125, 316, 345
BanII	GRGCYC	3 cut(s)	18, 571, 1488
BauI	CACGAG	1 cut(s)	723
BbsI	GAAGAC	1 cut(s)	849
Bbv12I	GWGCWC	3 cut(s)	18, 1077, 1710
BbvCI	CCTCAGC	1 cut(s)	489
BbvI	GCAGC	4 cut(s)	59, 67, 475, 1597
BccI	CCATC	9 cut(s)	167, 360, 598, 800, 1301, 1465, 1708, 1768, 1925
BceAI	ACGGC	3 cut(s)	79, 304, 1820
BcgI	CGANNNNNNTGC	2 cut(s)	61, 95
BciT130I	CCWGG	1 cut(s)	1902
BciVI	GTATCC	1 cut(s)	1106
BcoDI	GTCTC	2 cut(s)	910, 1175
BfaI	CTAG	2 cut(s)	273, 1958
BfmI	CTRYAG	2 cut(s)	533, 621
BfoI	RGCGCY	3 cut(s)	129, 349, 1480
BfrI	CTTAAG	1 cut(s)	1154
BfuAI	ACCTGC	1 cut(s)	1719
BfuI	GTATCC	1 cut(s)	1106
BglII	AGATCT	2 cut(s)	727, 1289
BisI	GCNGC	5 cut(s)	48, 56, 94, 464, 1586
BlsI	GCNGC	5 cut(s)	49, 57, 95, 465, 1587
Bme1390I	CCNGG	1 cut(s)	1902
Bme18I	GGWCC	3 cut(s)	336, 922, 1281
BmeT110I	CYCGRG	1 cut(s)	777
BmgBI	CACGTC	1 cut(s)	240
BmgT120I	GGNCC	5 cut(s)	336, 922, 1281, 1484, 1485
BmiI	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1486
BmrFI	CCNGG	1 cut(s)	1902
BmrI	ACTGGG	1 cut(s)	1439
BmsI	GCATC	6 cut(s)	69, 484, 893, 1822, 1837, 1876
BmuI	ACTGGG	1 cut(s)	1439
BoxI	GACNNNNGTC	1 cut(s)	1932
BpiI	GAAGAC	1 cut(s)	849
BpmI	CTGGAG	1 cut(s)	959
Bpu10I	CCTNAGC	1 cut(s)	489
Bpu14I	TTCGAA	1 cut(s)	896
BsaBI	GATNNNNATC	2 cut(s)	630, 1317
BsaHI	GRCGYC	3 cut(s)	36, 126, 346
BsaJI	CCNNGG	4 cut(s)	129, 144, 563, 1480
BsaWI	WCCGGW	2 cut(s)	10, 919
BsaXI	ACNNNNNCTCC	5 cut(s)	11, 30, 41, 1670, 1700
Bsc4I	CCNNNNNNNGG	5 cut(s)	254, 273, 362, 1219, 1842
Bse1I	ACTGG	4 cut(s)	400, 942, 954, 1434
Bse3DI	GCAATG	2 cut(s)	1167, 1565
Bse8I	GATNNNNATC	2 cut(s)	630, 1317
BseBI	CCWGG	1 cut(s)	1902
BseDI	CCNNGG	4 cut(s)	129, 144, 563, 1480
BseGI	GGATG	8 cut(s)	159, 371, 499, 658, 884, 1318, 1441, 1852
BseJI	GATNNNNATC	2 cut(s)	630, 1317
BseLI	CCNNNNNNNGG	5 cut(s)	254, 273, 362, 1219, 1842
BseMI	GCAATG	2 cut(s)	1167, 1565
BseMII	CTCAG	6 cut(s)	273, 480, 618, 981, 1695, 1802
BseNI	ACTGG	4 cut(s)	400, 942, 954, 1434
BseRI	GAGGAG	1 cut(s)	447
BseSI	GKGCMC	1 cut(s)	1488
BseXI	GCAGC	4 cut(s)	59, 67, 475, 1597
BseYI	CCCAGC	2 cut(s)	1108, 1620
BsgI	GTGCAG	3 cut(s)	342, 705, 1697
Bsh1236I	CGCG	1 cut(s)	179
BshFI	GGCC	4 cut(s)	810, 1099, 1257, 1486
BshNI	GGYRCC	3 cut(s)	125, 316, 345
BsiHKAI	GWGCWC	3 cut(s)	18, 1077, 1710
BsiHKCI	CYCGRG	1 cut(s)	777
BsiSI	CCGG	2 cut(s)	11, 920
BslFI	GGGAC	2 cut(s)	908, 1396
BslI	CCNNNNNNNGG	5 cut(s)	254, 273, 362, 1219, 1842
BsmAI	GTCTC	2 cut(s)	910, 1175
BsmFI	GGGAC	2 cut(s)	908, 1396
BsmI	GAATGC	1 cut(s)	1893
BsnI	GGCC	4 cut(s)	810, 1099, 1257, 1486
BsoBI	CYCGRG	1 cut(s)	777
Bsp119I	TTCGAA	1 cut(s)	896
Bsp120I	GGGCCC	1 cut(s)	1484
Bsp1286I	GDGCHC	5 cut(s)	18, 571, 1077, 1488, 1710
Bsp143I	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
BspACI	CCGC	4 cut(s)	93, 195, 1021, 1618
BspANI	GGCC	4 cut(s)	810, 1099, 1257, 1486
BspCNI	CTCAG	6 cut(s)	274, 481, 617, 982, 1696, 1803
BspFNI	CGCG	1 cut(s)	179
BspLI	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1486
BspMAI	CTGCAG	1 cut(s)	537
BspMI	ACCTGC	1 cut(s)	1719
BspPI	GGATC	3 cut(s)	710, 926, 1031
BspQI	GCTCTTC	2 cut(s)	112, 229
BspT104I	TTCGAA	1 cut(s)	896
BspT107I	GGYRCC	3 cut(s)	125, 316, 345
BspTI	CTTAAG	1 cut(s)	1154
BsrDI	GCAATG	2 cut(s)	1167, 1565
BsrI	ACTGG	4 cut(s)	400, 942, 954, 1434
BssECI	CCNNGG	4 cut(s)	129, 144, 563, 1480
BssMI	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
BssNI	GRCGYC	3 cut(s)	36, 126, 346
BssSI	CACGAG	1 cut(s)	723
BssT1I	CCWWGG	3 cut(s)	144, 563, 1480
Bst2BI	CACGAG	1 cut(s)	723
Bst2UI	CCWGG	1 cut(s)	1902
Bst4CI	ACNGT	6 cut(s)	1186, 1388, 1499, 1635, 1686, 1883
Bst6I	CTCTTC	3 cut(s)	112, 229, 803
BstACI	GRCGYC	3 cut(s)	36, 126, 346
BstAFI	CTTAAG	1 cut(s)	1154
BstBI	TTCGAA	1 cut(s)	896
BstC8I	GCNNGC	6 cut(s)	73, 116, 177, 1221, 1514, 1891
BstDEI	CTNAG	7 cut(s)	282, 489, 604, 990, 1539, 1704, 1811
BstF5I	GGATG	8 cut(s)	159, 371, 499, 658, 884, 1318, 1441, 1852
BstFNI	CGCG	1 cut(s)	179
BstH2I	RGCGCY	3 cut(s)	129, 349, 1480
BstHHI	GCGC	3 cut(s)	128, 348, 1479
BstKTI	GATC	7 cut(s)	718, 730, 934, 1026, 1292, 1378, 1405
BstMAI	GTCTC	2 cut(s)	910, 1175
BstMBI	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
BstMWI	GCNNNNNNNGC	8 cut(s)	44, 72, 102, 111, 214, 494, 1105, 1483
BstNI	CCWGG	1 cut(s)	1902
BstNSI	RCATGY	2 cut(s)	470, 1516
BstPAI	GACNNNNGTC	1 cut(s)	1932
BstSCI	CCNGG	1 cut(s)	1900
BstSFI	CTRYAG	2 cut(s)	533, 621
BstSLI	GKGCMC	1 cut(s)	1488
BstUI	CGCG	1 cut(s)	179
BstV1I	GCAGC	4 cut(s)	59, 67, 475, 1597
BstV2I	GAAGAC	1 cut(s)	849
BstX2I	RGATCY	3 cut(s)	727, 931, 1289
BstXI	CCANNNNNNTGG	1 cut(s)	818
BstYI	RGATCY	3 cut(s)	727, 931, 1289
BsuI	GTATCC	1 cut(s)	1106
BsuRI	GGCC	4 cut(s)	810, 1099, 1257, 1486
BtrI	CACGTC	1 cut(s)	240
BtsCI	GGATG	8 cut(s)	159, 371, 499, 658, 884, 1318, 1441, 1852
BtsI	GCAGTG	2 cut(s)	1202, 1916
BtsIMutI	CAGTG	4 cut(s)	935, 947, 1202, 1916
BveI	ACCTGC	1 cut(s)	1719
Cac8I	GCNNGC	6 cut(s)	73, 116, 177, 1221, 1514, 1891
CfoI	GCGC	3 cut(s)	128, 348, 1479
Cfr13I	GGNCC	5 cut(s)	336, 922, 1281, 1484, 1485
CseI	GACGC	3 cut(s)	39, 44, 168
Csp6I	GTAC	6 cut(s)	373, 418, 997, 1427, 1682, 1942
CviAII	CATG	8 cut(s)	76, 119, 172, 467, 1027, 1118, 1340, 1513
CviQI	GTAC	6 cut(s)	373, 418, 997, 1427, 1682, 1942
DdeI	CTNAG	7 cut(s)	282, 489, 604, 990, 1539, 1704, 1811
DinI	GGCGCC	2 cut(s)	127, 347
DpnI	GATC	7 cut(s)	717, 729, 933, 1025, 1291, 1377, 1404
DpnII	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
DraI	TTTAAA	1 cut(s)	1639
EaeI	YGGCCR	2 cut(s)	808, 1097
Eam1104I	CTCTTC	3 cut(s)	112, 229, 803
EarI	CTCTTC	3 cut(s)	112, 229, 803
EciI	GGCGGA	1 cut(s)	1036
Ecl136II	GAGCTC	1 cut(s)	16
Eco130I	CCWWGG	3 cut(s)	144, 563, 1480
Eco147I	AGGCCT	1 cut(s)	1257
Eco24I	GRGCYC	3 cut(s)	18, 571, 1488
Eco47I	GGWCC	3 cut(s)	336, 922, 1281
Eco53kI	GAGCTC	1 cut(s)	16
Eco57I	CTGAAG	2 cut(s)	1035, 1814
Eco88I	CYCGRG	1 cut(s)	777
EcoICRI	GAGCTC	1 cut(s)	16
EcoRI	GAATTC	3 cut(s)	294, 943, 1652
EcoRII	CCWGG	1 cut(s)	1900
EcoT14I	CCWWGG	3 cut(s)	144, 563, 1480
EcoT22I	ATGCAT	1 cut(s)	1123
EcoT38I	GRGCYC	3 cut(s)	18, 571, 1488
EgeI	GGCGCC	2 cut(s)	127, 347
EheI	GGCGCC	2 cut(s)	127, 347
ErhI	CCWWGG	3 cut(s)	144, 563, 1480
FaeI	CATG	8 cut(s)	79, 122, 175, 470, 1030, 1121, 1343, 1516
FaqI	GGGAC	2 cut(s)	908, 1396
FatI	CATG	8 cut(s)	75, 118, 171, 466, 1026, 1117, 1339, 1512
FauNDI	CATATG	3 cut(s)	1123, 1135, 1865
Fnu4HI	GCNGC	5 cut(s)	48, 56, 94, 464, 1586
FokI	GGATG	8 cut(s)	146, 378, 506, 665, 871, 1325, 1448, 1859
FriOI	GRGCYC	3 cut(s)	18, 571, 1488
Fsp4HI	GCNGC	5 cut(s)	48, 56, 94, 464, 1586
FspBI	CTAG	2 cut(s)	273, 1958
GlaI	GCGC	3 cut(s)	127, 347, 1478
GluI	GCNGC	5 cut(s)	48, 56, 94, 464, 1586
GsaI	CCCAGC	2 cut(s)	1112, 1624
GsuI	CTGGAG	1 cut(s)	959
HaeII	RGCGCY	3 cut(s)	129, 349, 1480
HaeIII	GGCC	4 cut(s)	810, 1099, 1257, 1486
HapII	CCGG	2 cut(s)	11, 920
HgaI	GACGC	3 cut(s)	39, 44, 168
HhaI	GCGC	3 cut(s)	128, 348, 1479
Hin1I	GRCGYC	3 cut(s)	36, 126, 346
Hin1II	CATG	8 cut(s)	79, 122, 175, 470, 1030, 1121, 1343, 1516
Hin6I	GCGC	3 cut(s)	126, 346, 1477
HinP1I	GCGC	3 cut(s)	126, 346, 1477
HincII	GTYRAC	2 cut(s)	324, 1927
HindII	GTYRAC	2 cut(s)	324, 1927
HindIII	AAGCTT	1 cut(s)	1038
HpaII	CCGG	2 cut(s)	11, 920
HphI	GGTGA	4 cut(s)	775, 1288, 1886, 1895
Hpy166II	GTNNAC	6 cut(s)	324, 587, 999, 1233, 1689, 1927
Hpy188III	TCNNGA	4 cut(s)	635, 779, 1189, 1829
Hpy8I	GTNNAC	6 cut(s)	324, 587, 999, 1233, 1689, 1927
Hpy99I	CGWCG	4 cut(s)	35, 38, 55, 184
HpyAV	CCTTC	8 cut(s)	116, 831, 1010, 1012, 1081, 1162, 1221, 1309
HpyCH4III	ACNGT	6 cut(s)	1186, 1388, 1499, 1635, 1686, 1883
HpyCH4IV	ACGT	1 cut(s)	239
HpyF10VI	GCNNNNNNNGC	8 cut(s)	44, 72, 102, 111, 214, 494, 1105, 1483
HpyF3I	CTNAG	7 cut(s)	282, 489, 604, 990, 1539, 1704, 1811
HpySE526I	ACGT	1 cut(s)	239
Hsp92I	GRCGYC	3 cut(s)	36, 126, 346
Hsp92II	CATG	8 cut(s)	79, 122, 175, 470, 1030, 1121, 1343, 1516
HspAI	GCGC	3 cut(s)	126, 346, 1477
KasI	GGCGCC	2 cut(s)	125, 345
Kzo9I	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
LguI	GCTCTTC	2 cut(s)	112, 229
LmnI	GCTCC	4 cut(s)	13, 21, 1474, 1768
Lsp1109I	GCAGC	4 cut(s)	59, 67, 475, 1597
LweI	GCATC	6 cut(s)	69, 484, 893, 1822, 1837, 1876
MaeI	CTAG	2 cut(s)	273, 1958
MaeII	ACGT	1 cut(s)	239
MaeIII	GTNAC	5 cut(s)	154, 763, 1382, 1499, 1589
MalI	GATC	7 cut(s)	717, 729, 933, 1025, 1291, 1377, 1404
MboI	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
MboII	GAAGA	6 cut(s)	99, 216, 790, 849, 1177, 1397
MflI	RGATCY	3 cut(s)	727, 931, 1289
MhlI	GDGCHC	5 cut(s)	18, 571, 1077, 1488, 1710
MlsI	TGGCCA	2 cut(s)	810, 1099
MluNI	TGGCCA	2 cut(s)	810, 1099
Mly113I	GGCGCC	2 cut(s)	126, 346
MlyI	GAGTC	4 cut(s)	444, 623, 992, 1448
MmeI	TCCRAC	5 cut(s)	54, 162, 291, 726, 1536
Mox20I	TGGCCA	2 cut(s)	810, 1099
Mph1103I	ATGCAT	1 cut(s)	1123
MroXI	GAANNNNTTC	1 cut(s)	1604
MscI	TGGCCA	2 cut(s)	810, 1099
MseI	TTAA	5 cut(s)	1043, 1155, 1464, 1494, 1638
MslI	CAYNNNNRTG	6 cut(s)	364, 816, 952, 1122, 1453, 1719
Msp20I	TGGCCA	2 cut(s)	810, 1099
MspA1I	CMGCKG	2 cut(s)	488, 1620
MspCI	CTTAAG	1 cut(s)	1154
MspI	CCGG	2 cut(s)	11, 920
MspR9I	CCNGG	1 cut(s)	1902
Mva1269I	GAATGC	1 cut(s)	1893
MvaI	CCWGG	1 cut(s)	1902
MvnI	CGCG	1 cut(s)	179
MwoI	GCNNNNNNNGC	8 cut(s)	44, 72, 102, 111, 214, 494, 1105, 1483
NarI	GGCGCC	2 cut(s)	126, 346
NdeI	CATATG	3 cut(s)	1123, 1135, 1865
NdeII	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
NlaIII	CATG	8 cut(s)	79, 122, 175, 470, 1030, 1121, 1343, 1516
NlaIV	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1486
NmeAIII	GCCGAG	2 cut(s)	47, 154
NmuCI	GTSAC	4 cut(s)	154, 763, 1499, 1589
NsiI	ATGCAT	1 cut(s)	1123
NspI	RCATGY	2 cut(s)	470, 1516
NspV	TTCGAA	1 cut(s)	896
PaeI	GCATGC	1 cut(s)	1516
PaeR7I	CTCGAG	1 cut(s)	777
PaqCI	CACCTGC	1 cut(s)	1719
PceI	AGGCCT	1 cut(s)	1257
PciSI	GCTCTTC	2 cut(s)	112, 229
PcsI	WCGNNNNNNNCGW	1 cut(s)	27
PctI	GAATGC	1 cut(s)	1893
PdmI	GAANNNNTTC	1 cut(s)	1604
PfeI	GAWTC	7 cut(s)	244, 893, 1010, 1031, 1127, 1330, 1445
PkrI	GCNGC	5 cut(s)	49, 57, 95, 465, 1587
PleI	GAGTC	4 cut(s)	443, 622, 991, 1447
PluTI	GGCGCC	2 cut(s)	129, 349
PpsI	GAGTC	4 cut(s)	443, 622, 991, 1447
PshAI	GACNNNNGTC	1 cut(s)	1932
Psp124BI	GAGCTC	1 cut(s)	18
Psp6I	CCWGG	1 cut(s)	1900
PspFI	CCCAGC	2 cut(s)	1108, 1620
PspGI	CCWGG	1 cut(s)	1900
PspN4I	GGNNCC	6 cut(s)	9, 127, 318, 347, 924, 1486
PspOMI	GGGCCC	1 cut(s)	1484
PspPI	GGNCC	5 cut(s)	336, 922, 1281, 1484, 1485
PstI	CTGCAG	1 cut(s)	537
PsuI	RGATCY	3 cut(s)	727, 931, 1289
PvuII	CAGCTG	1 cut(s)	488
RsaI	GTAC	6 cut(s)	374, 419, 998, 1428, 1683, 1943
RsaNI	GTAC	6 cut(s)	373, 418, 997, 1427, 1682, 1942
RseI	CAYNNNNRTG	6 cut(s)	364, 816, 952, 1122, 1453, 1719
SacI	GAGCTC	1 cut(s)	18
SapI	GCTCTTC	2 cut(s)	112, 229
SaqAI	TTAA	5 cut(s)	1043, 1155, 1464, 1494, 1638
SatI	GCNGC	5 cut(s)	48, 56, 94, 464, 1586
Sau3AI	GATC	7 cut(s)	715, 727, 931, 1023, 1289, 1375, 1402
Sau96I	GGNCC	5 cut(s)	336, 922, 1281, 1484, 1485
SchI	GAGTC	4 cut(s)	444, 623, 992, 1448
ScrFI	CCNGG	1 cut(s)	1902
SduI	GDGCHC	5 cut(s)	18, 571, 1077, 1488, 1710
SfaNI	GCATC	6 cut(s)	69, 484, 893, 1822, 1837, 1876
SfcI	CTRYAG	2 cut(s)	533, 621
SfoI	GGCGCC	2 cut(s)	127, 347
Sfr274I	CTCGAG	1 cut(s)	777
SfuI	TTCGAA	1 cut(s)	896
SinI	GGWCC	3 cut(s)	336, 922, 1281
SlaI	CTCGAG	1 cut(s)	777
SmiMI	CAYNNNNRTG	6 cut(s)	364, 816, 952, 1122, 1453, 1719
SmlI	CTYRAG	2 cut(s)	777, 1154
SmoI	CTYRAG	2 cut(s)	777, 1154
SphI	GCATGC	1 cut(s)	1516
SseBI	AGGCCT	1 cut(s)	1257
SsiI	CCGC	4 cut(s)	93, 195, 1021, 1618
SspDI	GGCGCC	2 cut(s)	125, 345
SspI	AATATT	2 cut(s)	1056, 1419
SspMI	CTAG	2 cut(s)	273, 1958
SstI	GAGCTC	1 cut(s)	18
StuI	AGGCCT	1 cut(s)	1257
StyD4I	CCNGG	1 cut(s)	1900
StyI	CCWWGG	3 cut(s)	144, 563, 1480
TaaI	ACNGT	6 cut(s)	1186, 1388, 1499, 1635, 1686, 1883
TaiI	ACGT	1 cut(s)	242
TaqI	TCGA	6 cut(s)	110, 778, 896, 1374, 1656, 1782
TaqII	GACCGA	2 cut(s)	324, 1298
TatI	WGTACW	3 cut(s)	372, 417, 1426
TauI	GCSGC	1 cut(s)	96
TfiI	GAWTC	7 cut(s)	244, 893, 1010, 1031, 1127, 1330, 1445
Tru1I	TTAA	5 cut(s)	1043, 1155, 1464, 1494, 1638
Tru9I	TTAA	5 cut(s)	1043, 1155, 1464, 1494, 1638
TscAI	CASTG	4 cut(s)	942, 954, 1202, 1923
TseFI	GTSAC	4 cut(s)	154, 763, 1499, 1589
TseI	GCWGC	4 cut(s)	47, 55, 463, 1585
Tsp45I	GTSAC	4 cut(s)	154, 763, 1499, 1589
TspDTI	ATGAA	6 cut(s)	135, 537, 642, 1178, 1789, 1821
TspGWI	ACGGA	2 cut(s)	791, 1099
TspRI	CASTG	4 cut(s)	942, 954, 1202, 1923
Vha464I	CTTAAG	1 cut(s)	1154
VpaK11BI	GGWCC	3 cut(s)	336, 922, 1281
XapI	RAATTY	7 cut(s)	294, 752, 770, 943, 1238, 1646, 1652
XceI	RCATGY	2 cut(s)	470, 1516
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1540
XhoI	CTCGAG	1 cut(s)	777
XmnI	GAANNNNTTC	1 cut(s)	1604
XspI	CTAG	2 cut(s)	273, 1958
Zsp2I	ATGCAT	1 cut(s)	1123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.