AT5G53040

RWP-RK domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012960
Physical Location & Seq
Reverse (-)
21506967 .. 21507948
982 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53040.1

Sequence Viewer

Length: 771 bp
ATGAGTTCGTCAAAACATTCCTCTGTTTTTAACTATTCTGCTCTGTTTCTATCACTGTTTCTTCAACAAATGGATCAGAACTCTCTTCATCATCTCGATTCTCCAAAAATCGAAAACGAGTATGAACCAGATTCGTTATACGACATGTTAGATAAGTTGCCTCCGCTTGATTCTCTCCTAGATATGGAAGATTTGAAACCAAATGCAGGGTTGCACTTTCAGTTCCATTACAATAGCTTTGAAGATTTCTTCGAAAACATTGAAGTGGATAACACAATTCCATCTGATATTCACTTGTTGACACAAGAGCCCTACTTCTCAAGTGACTCCTCTTCCTCTTCACCATTGGCTATCCAAAACGACGGTCTCATTTCCAACGTGAAAGTTGAAAAGGTAACAGTTAAGAAGAAGAGGAACCTTAAGAAAAAGAGGCAAGACAAATTGGAGATGTCTGAGATCAAACAATTTTTCGATAGGCCGATCATGAAAGCGGCTAAAGAACTGAACGTGGGACTCACTGTGTTGAAGAAGCGATGCAGGGAATTAGGAATTTACCGGTGGCCTCACCGGAAGCTCAAGAGTCTAAACTCTCTTATAAAGAATCTCAAGAATGTTGGAATGGAAGAGGAAGTGAAGAACTTGGAGGAACATAGGTTTCTTATTGAACAAGAACCTGATGCAGAACTCAGTGATGGAACCAAGAAGCTAAGGCAAGCTTGTTTCAAAGCCAATTATAAGAGAAGAAAATCACTTGGTGATGATTATTATTGA

Protein Analysis

256

Amino Acids

29.9

Weight (kDa)

6.72

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 148 - 195 5.6e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 596, 735
AciI CCGC 2 cut(s) 164, 491
AclWI GGATC 1 cut(s) 81
AcsI RAATTY 1 cut(s) 549
AdeI CACNNNGTG 1 cut(s) 755
AfiI CCNNNNNNNGG 2 cut(s) 184, 206
AflII CTTAAG 1 cut(s) 419
AflIII ACRYGT 1 cut(s) 144
AgeI ACCGGT 1 cut(s) 555
AgsI TTSAA 8 cut(s) 65, 196, 242, 263, 389, 526, 665, 724
AluBI AGCT 4 cut(s) 237, 574, 706, 716
AluI AGCT 4 cut(s) 237, 574, 706, 716
Alw26I GTCTC 1 cut(s) 371
AlwI GGATC 1 cut(s) 81
AoxI GGCC 2 cut(s) 476, 560
ApoI RAATTY 1 cut(s) 549
ArsI GACNNNNNNTTYG 2 cut(s) 349, 381
AsiGI ACCGGT 1 cut(s) 555
AsuHPI GGTGA 3 cut(s) 333, 557, 767
AsuII TTCGAA 1 cut(s) 252
BanII GRGCYC 1 cut(s) 312
BccI CCATC 2 cut(s) 289, 686
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 179
BfrI CTTAAG 1 cut(s) 419
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
BmiI GGNNCC 2 cut(s) 416, 697
BmsI GCATC 2 cut(s) 524, 667
Bpu10I CCTNAGC 1 cut(s) 707
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 3 cut(s) 304, 560, 590
BsaI GGTCTC 1 cut(s) 371
BsaWI WCCGGW 2 cut(s) 555, 567
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 206
Bse118I RCCGGY 1 cut(s) 555
BseLI CCNNNNNNNGG 2 cut(s) 184, 206
BseMII CTCAG 2 cut(s) 444, 700
BseRI GAGGAG 1 cut(s) 319
BshFI GGCC 2 cut(s) 478, 562
BshTI ACCGGT 1 cut(s) 555
BsiSI CCGG 2 cut(s) 556, 568
BslFI GGGAC 1 cut(s) 525
BslI CCNNNNNNNGG 2 cut(s) 184, 206
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 525
BsnI GGCC 2 cut(s) 478, 562
Bso31I GGTCTC 1 cut(s) 371
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 3 cut(s) 73, 456, 480
BspACI CCGC 2 cut(s) 164, 491
BspANI GGCC 2 cut(s) 478, 562
BspCNI CTCAG 2 cut(s) 445, 699
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 2 cut(s) 416, 697
BspPI GGATC 1 cut(s) 81
BspT104I TTCGAA 1 cut(s) 252
BspTI CTTAAG 1 cut(s) 419
BspTNI GGTCTC 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 555
BssAI RCCGGY 1 cut(s) 555
BssMI GATC 3 cut(s) 73, 456, 480
Bst4CI ACNGT 4 cut(s) 57, 365, 400, 520
Bst6I CTCTTC 5 cut(s) 90, 337, 343, 404, 618
BstAFI CTTAAG 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 3 cut(s) 453, 686, 707
BstKTI GATC 3 cut(s) 76, 459, 483
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 3 cut(s) 73, 456, 480
BstNSI RCATGY 1 cut(s) 148
BsuRI GGCC 2 cut(s) 478, 562
BtgZI GCGATG 1 cut(s) 547
BtsIMutI CAGTG 3 cut(s) 53, 516, 694
Cac8I GCNNGC 1 cut(s) 714
CciI TCATGA 1 cut(s) 483
Cfr10I RCCGGY 1 cut(s) 555
CspAI ACCGGT 1 cut(s) 555
CviAII CATG 2 cut(s) 145, 484
DdeI CTNAG 3 cut(s) 453, 686, 707
DpnI GATC 3 cut(s) 75, 458, 482
DpnII GATC 3 cut(s) 73, 456, 480
DraIII CACNNNGTG 1 cut(s) 755
Eam1104I CTCTTC 5 cut(s) 90, 337, 343, 404, 618
EarI CTCTTC 5 cut(s) 90, 337, 343, 404, 618
Eco24I GRGCYC 1 cut(s) 312
Eco31I GGTCTC 1 cut(s) 371
EcoT38I GRGCYC 1 cut(s) 312
FaeI CATG 2 cut(s) 148, 487
FaiI YATR 8 cut(s) 123, 139, 146, 185, 485, 596, 651, 735
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FaqI GGGAC 1 cut(s) 525
FatI CATG 2 cut(s) 144, 483
Fnu4HI GCNGC 1 cut(s) 492
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 1 cut(s) 179
GluI GCNGC 1 cut(s) 492
HaeIII GGCC 2 cut(s) 478, 562
HapII CCGG 2 cut(s) 556, 568
Hin1II CATG 2 cut(s) 148, 487
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 7 cut(s) 98, 131, 170, 326, 513, 580, 601
HpaII CCGG 2 cut(s) 556, 568
HphI GGTGA 3 cut(s) 333, 557, 767
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 3 cut(s) 78, 286, 454
Hpy188III TCNNGA 4 cut(s) 95, 484, 577, 607
Hpy8I GTNNAC 1 cut(s) 300
Hpy99I CGWCG 1 cut(s) 365
HpyCH4III ACNGT 4 cut(s) 57, 365, 400, 520
HpyCH4IV ACGT 2 cut(s) 378, 507
HpyCH4V TGCA 4 cut(s) 206, 214, 537, 680
HpyF3I CTNAG 3 cut(s) 453, 686, 707
HpySE526I ACGT 2 cut(s) 378, 507
Hsp92II CATG 2 cut(s) 148, 487
Kzo9I GATC 3 cut(s) 73, 456, 480
LpnPI CCDG 6 cut(s) 141, 192, 523, 569, 581, 687
LweI GCATC 2 cut(s) 524, 667
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 378, 507
MaeIII GTNAC 2 cut(s) 323, 394
MalI GATC 3 cut(s) 75, 458, 482
MboI GATC 3 cut(s) 73, 456, 480
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 6 cut(s) 276, 440, 464, 542, 549, 730
MlyI GAGTC 3 cut(s) 320, 507, 589
MmeI TCCRAC 2 cut(s) 399, 595
MnlI CCTC 9 cut(s) 31, 171, 340, 346, 405, 423, 573, 619, 637
MseI TTAA 3 cut(s) 30, 402, 420
MslI CAYNNNNRTG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 2 cut(s) 556, 568
NdeII GATC 3 cut(s) 73, 456, 480
NlaIII CATG 2 cut(s) 148, 487
NlaIV GGNNCC 2 cut(s) 416, 697
NmuCI GTSAC 1 cut(s) 323
NspI RCATGY 1 cut(s) 148
NspV TTCGAA 1 cut(s) 252
PagI TCATGA 1 cut(s) 483
PciI ACATGT 1 cut(s) 144
PfeI GAWTC 4 cut(s) 98, 131, 170, 601
PinAI ACCGGT 1 cut(s) 555
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 3 cut(s) 320, 507, 588
PpsI GAGTC 3 cut(s) 320, 507, 588
PscI ACATGT 1 cut(s) 144
PsiI TTATAA 2 cut(s) 596, 735
PspN4I GGNNCC 2 cut(s) 416, 697
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 3 cut(s) 30, 402, 420
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 3 cut(s) 73, 456, 480
SchI GAGTC 3 cut(s) 320, 507, 589
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 2 cut(s) 524, 667
SfuI TTCGAA 1 cut(s) 252
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 4 cut(s) 319, 419, 575, 605
SmoI CTYRAG 4 cut(s) 319, 419, 575, 605
Sse9I AATT 6 cut(s) 276, 440, 464, 542, 549, 730
SsiI CCGC 2 cut(s) 164, 491
SspMI CTAG 1 cut(s) 179
TaaI ACNGT 4 cut(s) 57, 365, 400, 520
TaiI ACGT 2 cut(s) 381, 510
TaqI TCGA 4 cut(s) 96, 111, 252, 471
TasI AATT 6 cut(s) 276, 440, 464, 542, 549, 730
TauI GCSGC 1 cut(s) 494
TfiI GAWTC 4 cut(s) 98, 131, 170, 601
Tru1I TTAA 3 cut(s) 30, 402, 420
Tru9I TTAA 3 cut(s) 30, 402, 420
TscAI CASTG 3 cut(s) 60, 523, 694
TseFI GTSAC 1 cut(s) 323
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 3 cut(s) 77, 138, 500
TspRI CASTG 3 cut(s) 60, 523, 694
Vha464I CTTAAG 1 cut(s) 419
XapI RAATTY 1 cut(s) 549
XceI RCATGY 1 cut(s) 148
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.