RchiOBHm_Chr1g0380421

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012960
Physical Location & Seq
Forward (+)
66096748 .. 66098169
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60369

Sequence Viewer

Length: 1050 bp
ATGGGTTTTCAAGGACTTGAAGATTTCAGCACCTGGGACTTTACTGCTTTGGACATCACCACTGCTGTTTCATCACCATCTCGTTGTGATAATCATGTGCTTGATGAAAAGCCTCTTCCAACAATGGTGGATTCTTTTGACTACTTGGACAATGTCCCAAGTGATTCTGGTCATGGTGAAGTTTCATTCAAGATTGAAACATCTGGTTTTTCATCATCTCATTGTGATTCTTTGGTGTTTGATGAAAAGCCTCTTACAACAATGGTGGAGTCCTTTACCTACTTGGACAATGTCCCAAGTGATTTGGGTCAGCATGAAGTTTCAATGATGACTGAAAGCTGTGGCTTTTCATCATCTCATTGTGATTCTGTGGTGTTCAATGAAAAGCCCATTTCAACAATGGTAGAGTCATTTGACAACTCAAACAATGTCCCGAGTGTTCTGGGTCCCCATGAAGCTTCCATGAGGACTGAAAGCGCTACTGCTTTTGCGTCGTCCCATAGTAATTCTCTGGTGCTTTATGAAAAACCCCTTTCAACAATCGTGGATTCTTTCGACTACTCTGATATTCCTACACCTGATTTGGGCCATGATGAAGTTTCAGTGATGATTGAAAATGGGTTTCCTAGTTGGGACATATCAGACATTAGTTGTAAAAGAGAGACAAGTAATAGAAGGAGATATAATACAGAGATGAAGATCAAGTCTGATTCTTTGGAATTTGAGGAGATAAAGAAGTACTTTGACCTCCCCATAACAAAAGCAGCCAAGGAACTGAAGGTGGGAGTGACTTTGTTGAAGAGGAGGTGCAGGGAGCTCAACATCATGAGGTGGCCTCACAGAAAGATTAGGAGCTTGAACTCTCTCATTGAAACTCTCAAGGGAATGGGTTTGAGAAATGAGGTAAAGATGCTGGAGGAGAACAAAAGGCTTCTAGAGCAAGTGCCAAACATGGAGCTAACAAAGAGAACCAAAAAGCTGAGGCAGTCTATTTTCAAAGCTAACTACAACTACAAAAAGCAGATGTCTTTGTCTTTGTTGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

349

Amino Acids

39.56

Weight (kDa)

5.31

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 238 - 286 9.1e-24 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 719
AcuI CTGAAG 1 cut(s) 797
AfaI GTAC 1 cut(s) 740
AfeI AGCGCT 1 cut(s) 478
AfiI CCNNNNNNNGG 1 cut(s) 584
AjnI CCWGG 1 cut(s) 32
AluBI AGCT 7 cut(s) 339, 458, 817, 855, 958, 979, 1001
AluI AGCT 7 cut(s) 339, 458, 817, 855, 958, 979, 1001
Alw21I GWGCWC 1 cut(s) 819
Alw26I GTCTC 1 cut(s) 656
AlwNI CAGNNNCTG 1 cut(s) 33
Ama87I CYCGRG 1 cut(s) 433
Aor51HI AGCGCT 1 cut(s) 478
AoxI GGCC 2 cut(s) 586, 833
ApeKI GCWGC 1 cut(s) 764
ApoI RAATTY 1 cut(s) 719
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 2 cut(s) 446, 586
AsuHPI GGTGA 3 cut(s) 49, 66, 188
AvaI CYCGRG 1 cut(s) 433
AvaII GGWCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 819
Bbv12I GWGCWC 1 cut(s) 819
BbvCI CCTCAGC 1 cut(s) 980
BbvI GCAGC 1 cut(s) 776
BccI CCATC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 656
BfaI CTAG 2 cut(s) 627, 935
BfoI RGCGCY 1 cut(s) 480
BisI GCNGC 1 cut(s) 765
BlsI GCNGC 1 cut(s) 766
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 1 cut(s) 34
Bme18I GGWCC 1 cut(s) 446
BmeT110I CYCGRG 1 cut(s) 433
BmgT120I GGNCC 2 cut(s) 446, 586
BmiI GGNNCC 2 cut(s) 447, 448
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 900
BplI GAGNNNNNCTC 2 cut(s) 820, 852
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 980
BpuEI CTTGAG 1 cut(s) 863
BsaBI GATNNNNATC 1 cut(s) 698
BsaJI CCNNGG 2 cut(s) 33, 768
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse8I GATNNNNATC 1 cut(s) 698
BseBI CCWGG 1 cut(s) 34
BseDI CCNNGG 2 cut(s) 33, 768
BseJI GATNNNNATC 1 cut(s) 698
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 1 cut(s) 971
BseRI GAGGAG 3 cut(s) 740, 817, 932
BseXI GCAGC 1 cut(s) 776
BsgI GTGCAG 1 cut(s) 829
BshFI GGCC 2 cut(s) 588, 835
BsiHKAI GWGCWC 1 cut(s) 819
BsiHKCI CYCGRG 1 cut(s) 433
BslFI GGGAC 7 cut(s) 50, 140, 278, 416, 432, 481, 647
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 7 cut(s) 50, 140, 278, 416, 432, 481, 647
BsnI GGCC 2 cut(s) 588, 835
BsoBI CYCGRG 1 cut(s) 433
Bsp1286I GDGCHC 1 cut(s) 819
Bsp143I GATC 1 cut(s) 699
BspANI GGCC 2 cut(s) 588, 835
BspCNI CTCAG 1 cut(s) 972
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 2 cut(s) 447, 448
BssECI CCNNGG 2 cut(s) 33, 768
BssMI GATC 1 cut(s) 699
BssT1I CCWWGG 1 cut(s) 768
Bst2UI CCWGG 1 cut(s) 34
Bst6I CTCTTC 2 cut(s) 120, 794
BstDEI CTNAG 1 cut(s) 980
BstH2I RGCGCY 1 cut(s) 480
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 1 cut(s) 702
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 1 cut(s) 699
BstMWI GCNNNNNNNGC 1 cut(s) 937
BstNI CCWGG 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 776
BsuRI GGCC 2 cut(s) 588, 835
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 2 cut(s) 60, 609
CaiI CAGNNNCTG 1 cut(s) 33
CciI TCATGA 1 cut(s) 825
CfoI GCGC 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 446, 586
CseI GACGC 1 cut(s) 480
Csp6I GTAC 1 cut(s) 739
CspCI CAANNNNNGTGG 6 cut(s) 108, 143, 246, 281, 525, 560
CviAII CATG 8 cut(s) 95, 173, 314, 452, 463, 590, 826, 952
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 1 cut(s) 980
DpnI GATC 1 cut(s) 701
DpnII GATC 1 cut(s) 699
Eam1104I CTCTTC 2 cut(s) 120, 794
EarI CTCTTC 2 cut(s) 120, 794
Ecl136II GAGCTC 1 cut(s) 817
Eco130I CCWWGG 1 cut(s) 768
Eco24I GRGCYC 1 cut(s) 819
Eco47I GGWCC 1 cut(s) 446
Eco47III AGCGCT 1 cut(s) 478
Eco53kI GAGCTC 1 cut(s) 817
Eco57I CTGAAG 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 433
EcoICRI GAGCTC 1 cut(s) 817
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 768
EcoT38I GRGCYC 1 cut(s) 819
ErhI CCWWGG 1 cut(s) 768
FaeI CATG 8 cut(s) 98, 176, 317, 455, 466, 593, 829, 955
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 7 cut(s) 50, 140, 278, 416, 432, 481, 647
FatI CATG 8 cut(s) 94, 172, 313, 451, 462, 589, 825, 951
Fnu4HI GCNGC 1 cut(s) 765
FriOI GRGCYC 1 cut(s) 819
Fsp4HI GCNGC 1 cut(s) 765
FspBI CTAG 2 cut(s) 627, 935
GlaI GCGC 1 cut(s) 478
GluI GCNGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 935
HaeII RGCGCY 1 cut(s) 480
HaeIII GGCC 2 cut(s) 588, 835
HgaI GACGC 1 cut(s) 480
HhaI GCGC 1 cut(s) 479
Hin1II CATG 8 cut(s) 98, 176, 317, 455, 466, 593, 829, 955
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HindIII AAGCTT 1 cut(s) 456
HinfI GANTC 8 cut(s) 131, 164, 227, 269, 365, 407, 548, 710
HphI GGTGA 3 cut(s) 49, 66, 188
Hpy188I TCNGA 3 cut(s) 565, 643, 709
Hpy188III TCNNGA 4 cut(s) 190, 433, 826, 935
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 2 cut(s) 669, 772
HpyCH4V TGCA 1 cut(s) 810
HpyF10VI GCNNNNNNNGC 1 cut(s) 937
HpyF3I CTNAG 1 cut(s) 980
Hsp92II CATG 8 cut(s) 98, 176, 317, 455, 466, 593, 829, 955
HspAI GCGC 1 cut(s) 477
KflI GGGWCCC 1 cut(s) 446
Kzo9I GATC 1 cut(s) 699
LmnI GCTCC 3 cut(s) 814, 852, 955
LpnPI CCDG 9 cut(s) 19, 46, 153, 189, 428, 497, 591, 796, 899
Lsp1109I GCAGC 1 cut(s) 776
LweI GCATC 1 cut(s) 900
MaeI CTAG 2 cut(s) 627, 935
MaeIII GTNAC 1 cut(s) 787
MalI GATC 1 cut(s) 701
MboI GATC 1 cut(s) 699
MboII GAAGA 4 cut(s) 32, 107, 709, 811
MhlI GDGCHC 1 cut(s) 819
MluCI AATT 2 cut(s) 505, 719
MlyI GAGTC 2 cut(s) 278, 416
MmeI TCCRAC 1 cut(s) 143
MspR9I CCNGG 1 cut(s) 34
MvaI CCWGG 1 cut(s) 34
MwoI GCNNNNNNNGC 1 cut(s) 937
NdeII GATC 1 cut(s) 699
NlaIII CATG 8 cut(s) 98, 176, 317, 455, 466, 593, 829, 955
NlaIV GGNNCC 2 cut(s) 447, 448
NmuCI GTSAC 1 cut(s) 787
PagI TCATGA 1 cut(s) 825
PfeI GAWTC 6 cut(s) 131, 164, 227, 365, 548, 710
PflFI GACNNNGTC 2 cut(s) 152, 290
PkrI GCNGC 1 cut(s) 766
PleI GAGTC 2 cut(s) 277, 415
PpsI GAGTC 2 cut(s) 277, 415
PpuMI RGGWCCY 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 819
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 32
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 2 cut(s) 447, 448
PspPI GGNCC 2 cut(s) 446, 586
PspPPI RGGWCCY 1 cut(s) 446
PstNI CAGNNNCTG 1 cut(s) 33
PsyI GACNNNGTC 2 cut(s) 152, 290
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
SacI GAGCTC 1 cut(s) 819
SatI GCNGC 1 cut(s) 765
Sau3AI GATC 1 cut(s) 699
Sau96I GGNCC 2 cut(s) 446, 586
ScaI AGTACT 1 cut(s) 740
SchI GAGTC 2 cut(s) 278, 416
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 1 cut(s) 819
SfaNI GCATC 1 cut(s) 900
SinI GGWCC 1 cut(s) 446
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
Sse9I AATT 2 cut(s) 505, 719
SspMI CTAG 2 cut(s) 627, 935
SstI GAGCTC 1 cut(s) 819
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 1 cut(s) 768
TaqI TCGA 1 cut(s) 555
TasI AATT 2 cut(s) 505, 719
TatI WGTACW 1 cut(s) 738
TfiI GAWTC 6 cut(s) 131, 164, 227, 365, 548, 710
TscAI CASTG 2 cut(s) 67, 609
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 1 cut(s) 764
Tsp45I GTSAC 1 cut(s) 787
TspRI CASTG 2 cut(s) 67, 609
Tth111I GACNNNGTC 2 cut(s) 152, 290
VpaK11BI GGWCC 1 cut(s) 446
XapI RAATTY 1 cut(s) 719
XbaI TCTAGA 1 cut(s) 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XspI CTAG 2 cut(s) 627, 935
ZrmI AGTACT 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.