pycom01g22770

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012960
Physical Location & Seq
Forward (+)
20266702 .. 20268196
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22770.3

Sequence Viewer

Length: 795 bp
ATGGCGGATTCCATTTTCTCCCAATATCTGGTTCCAAAAATTGAGACCGACCCACATGAGTTTAACTCCTCCATACCGTACTACCAGGACCCATGTCAGGATCAAAGTGCTGCGTTAGAAAGTCTACGGCTTGAAAATTTCAATCAGTTTACTTCACCCCCATTGCCGTTGCAATACAATCCCAACCAACTTCCACAAATGGGTTCTCAAGATTTTGAGGATTTAGGCATCTGGGACTATATTTTGGACGATGCTGCTCTTCCATCACCTTTTGATTACGAGGTGGTTGATGAAAATCCCCTGACAACAATGGCGGAATCCATCGTCTGTTCGAACAGTGTTTCGGGTGATTCGGGTGATGTTTTCAGGATGCTTGATTGGGGGATGGAAAGTTGGGGGAAATACGACGGGGAAGGCATGACAAAAAATGGGAGTTGCTCTAAGAGAATGTCCGCTGCTCCATTGGAATTGGACGAGATTCAGAAGTATTTCGACGTGCCGATAACTCAGGCGGCCAAGGAATTGAAGGTGGGATTAACGGTTTTGAAGAGGAGGTGCAGGGAACTGAATATCATGAGGTGGCCGCACAGAAAGCTCAGGAGCTTGAATGCTCTCATTGAAAATGTTAAGGGGATGGGATTGACGAATGAGCTGAGGATGCTGGAGGAGCACAAGAGGCTTTTAGAGCAGCTGCCGGACATGGAGTTGCCGGAGAGAGCCAAGAGGCTGAGGCAGGCTTGCTTCAAAGCCAACTACAAGAAGAAGAGGACTTTGTGTTTGACTGCCGGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

265

Amino Acids

30.21

Weight (kDa)

5.1

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 28
AccI GTMKAC 1 cut(s) 124
AciI CCGC 5 cut(s) 5, 314, 453, 512, 584
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 2 cut(s) 513, 581
AcsI RAATTY 1 cut(s) 136
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 3 cut(s) 28, 97, 200
AgsI TTSAA 7 cut(s) 134, 142, 526, 547, 607, 620, 745
AhdI GACNNNNNGTC 1 cut(s) 786
AjiI CACGTC 1 cut(s) 496
AjnI CCWGG 1 cut(s) 84
AjuI GAANNNNNNNTTGG 2 cut(s) 15, 47
AluBI AGCT 4 cut(s) 595, 603, 652, 691
AluI AGCT 4 cut(s) 595, 603, 652, 691
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 1 cut(s) 108
AoxI GGCC 2 cut(s) 513, 581
ApeKI GCWGC 5 cut(s) 110, 254, 455, 688, 691
ApoI RAATTY 1 cut(s) 136
ArsI GACNNNNNNTTYG 2 cut(s) 760, 792
Asp700I GAANNNNTTC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 88, 788
AsuHPI GGTGA 4 cut(s) 147, 258, 359, 368
AsuII TTCGAA 1 cut(s) 332
AvaII GGWCC 2 cut(s) 88, 788
Bbv12I GWGCWC 1 cut(s) 672
BbvCI CCTCAGC 2 cut(s) 653, 728
BbvI GCAGC 5 cut(s) 97, 241, 442, 678, 700
BccI CCATC 4 cut(s) 271, 329, 379, 628
BceAI ACGGC 2 cut(s) 143, 151
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 38
BisI GCNGC 7 cut(s) 111, 255, 456, 513, 584, 689, 692
BlsI GCNGC 7 cut(s) 112, 256, 457, 514, 585, 690, 693
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 2 cut(s) 88, 788
BmeRI GACNNNNNGTC 1 cut(s) 786
BmgBI CACGTC 1 cut(s) 496
BmgT120I GGNCC 2 cut(s) 88, 788
BmiI GGNNCC 2 cut(s) 33, 90
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 4 cut(s) 237, 241, 360, 648
BoxI GACNNNNGTC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 50, 82
BpmI CTGGAG 1 cut(s) 683
Bpu10I CCTNAGC 3 cut(s) 596, 653, 728
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 516
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 97, 200
Bse118I RCCGGY 1 cut(s) 785
Bse3DI GCAATG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 294
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 516
BseGI GGATG 4 cut(s) 375, 390, 639, 663
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 3 cut(s) 28, 97, 200
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 4 cut(s) 521, 610, 644, 719
BseRI GAGGAG 3 cut(s) 58, 565, 680
BseXI GCAGC 5 cut(s) 97, 241, 442, 678, 700
BsgI GTGCAG 1 cut(s) 577
BshFI GGCC 2 cut(s) 515, 583
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 3 cut(s) 695, 710, 786
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 3 cut(s) 28, 97, 200
BsmAI GTCTC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 2 cut(s) 515, 583
Bso31I GGTCTC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 672
Bsp143I GATC 1 cut(s) 100
BspACI CCGC 5 cut(s) 5, 314, 453, 512, 584
BspANI GGCC 2 cut(s) 515, 583
BspCNI CTCAG 4 cut(s) 520, 609, 645, 720
BspHI TCATGA 1 cut(s) 573
BspLI GGNNCC 2 cut(s) 33, 90
BspPI GGATC 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 264
BspT104I TTCGAA 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 785
BssAI RCCGGY 1 cut(s) 785
BssECI CCNNGG 1 cut(s) 516
BssMI GATC 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 516
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 3 cut(s) 78, 338, 541
Bst6I CTCTTC 3 cut(s) 264, 542, 758
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 735, 739
BstDEI CTNAG 5 cut(s) 441, 507, 596, 653, 728
BstF5I GGATG 4 cut(s) 375, 390, 639, 663
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 5 cut(s) 592, 658, 667, 676, 685
BstNI CCWGG 1 cut(s) 86
BstPAI GACNNNNGTC 1 cut(s) 93
BstSCI CCNGG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 97, 241, 442, 678, 700
BsuRI GGCC 2 cut(s) 515, 583
BtrI CACGTC 1 cut(s) 496
BtsCI GGATG 4 cut(s) 375, 390, 639, 663
BtsIMutI CAGTG 1 cut(s) 343
Cac8I GCNNGC 2 cut(s) 735, 739
CciI TCATGA 1 cut(s) 573
Cfr10I RCCGGY 1 cut(s) 785
Cfr13I GGNCC 2 cut(s) 88, 788
Csp6I GTAC 1 cut(s) 79
CviAII CATG 6 cut(s) 56, 93, 418, 574, 700, 792
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 5 cut(s) 441, 507, 596, 653, 728
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
DriI GACNNNNNGTC 1 cut(s) 786
EaeI YGGCCR 2 cut(s) 513, 581
Eam1104I CTCTTC 3 cut(s) 264, 542, 758
Eam1105I GACNNNNNGTC 1 cut(s) 786
EarI CTCTTC 3 cut(s) 264, 542, 758
EciI GGCGGA 2 cut(s) 20, 329
Eco130I CCWWGG 1 cut(s) 516
Eco31I GGTCTC 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 88, 788
EcoO109I RGGNCCY 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 6 cut(s) 59, 96, 421, 577, 703, 795
FaiI YATR 8 cut(s) 57, 74, 94, 240, 419, 575, 701, 793
FaqI GGGAC 1 cut(s) 248
FatI CATG 6 cut(s) 55, 92, 417, 573, 699, 791
FblI GTMKAC 1 cut(s) 124
Fnu4HI GCNGC 7 cut(s) 111, 255, 456, 513, 584, 689, 692
FokI GGATG 4 cut(s) 382, 397, 646, 670
Fsp4HI GCNGC 7 cut(s) 111, 255, 456, 513, 584, 689, 692
GluI GCNGC 7 cut(s) 111, 255, 456, 513, 584, 689, 692
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 2 cut(s) 515, 583
HapII CCGG 3 cut(s) 695, 710, 786
Hin1II CATG 6 cut(s) 59, 96, 421, 577, 703, 795
HinfI GANTC 4 cut(s) 8, 317, 350, 478
HpaII CCGG 3 cut(s) 695, 710, 786
HphI GGTGA 4 cut(s) 147, 258, 359, 368
Hpy166II GTNNAC 2 cut(s) 125, 150
Hpy188I TCNGA 1 cut(s) 483
Hpy188III TCNNGA 5 cut(s) 98, 209, 367, 574, 598
Hpy8I GTNNAC 2 cut(s) 125, 150
Hpy99I CGWCG 2 cut(s) 410, 497
HpyAV CCTTC 2 cut(s) 407, 520
HpyCH4III ACNGT 3 cut(s) 78, 338, 541
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 2 cut(s) 172, 558
HpyF10VI GCNNNNNNNGC 5 cut(s) 592, 658, 667, 676, 685
HpyF3I CTNAG 5 cut(s) 441, 507, 596, 653, 728
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 6 cut(s) 59, 96, 421, 577, 703, 795
Kzo9I GATC 1 cut(s) 100
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 3 cut(s) 463, 600, 667
Lsp1109I GCAGC 5 cut(s) 97, 241, 442, 678, 700
LweI GCATC 4 cut(s) 237, 241, 360, 648
MaeII ACGT 1 cut(s) 495
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 4 cut(s) 251, 559, 772, 775
MhlI GDGCHC 1 cut(s) 672
MluCI AATT 4 cut(s) 39, 136, 467, 521
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 3 cut(s) 63, 536, 627
MspA1I CMGCKG 2 cut(s) 455, 691
MspI CCGG 3 cut(s) 695, 710, 786
MspR9I CCNGG 1 cut(s) 86
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 1 cut(s) 86
MwoI GCNNNNNNNGC 5 cut(s) 592, 658, 667, 676, 685
NdeII GATC 1 cut(s) 100
NlaIII CATG 6 cut(s) 59, 96, 421, 577, 703, 795
NlaIV GGNNCC 2 cut(s) 33, 90
NspV TTCGAA 1 cut(s) 332
PagI TCATGA 1 cut(s) 573
PciSI GCTCTTC 1 cut(s) 264
PctI GAATGC 1 cut(s) 613
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 4 cut(s) 8, 317, 350, 478
PflMI CCANNNNNTGG 1 cut(s) 28
PkrI GCNGC 7 cut(s) 112, 256, 457, 514, 585, 690, 693
PpuMI RGGWCCY 1 cut(s) 88
PshAI GACNNNNGTC 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 33, 90
PspPI GGNCC 2 cut(s) 88, 788
PspPPI RGGWCCY 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 691
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 3 cut(s) 63, 536, 627
SatI GCNGC 7 cut(s) 111, 255, 456, 513, 584, 689, 692
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 2 cut(s) 88, 788
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 1 cut(s) 672
SfaNI GCATC 4 cut(s) 237, 241, 360, 648
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 2 cut(s) 88, 788
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
Sse9I AATT 4 cut(s) 39, 136, 467, 521
SsiI CCGC 5 cut(s) 5, 314, 453, 512, 584
StyD4I CCNGG 1 cut(s) 84
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 3 cut(s) 78, 338, 541
TaiI ACGT 1 cut(s) 498
TaqI TCGA 2 cut(s) 332, 492
TaqII GACCGA 1 cut(s) 62
TasI AATT 4 cut(s) 39, 136, 467, 521
TauI GCSGC 2 cut(s) 515, 586
TfiI GAWTC 4 cut(s) 8, 317, 350, 478
Tru1I TTAA 3 cut(s) 63, 536, 627
Tru9I TTAA 3 cut(s) 63, 536, 627
TscAI CASTG 1 cut(s) 343
TseI GCWGC 5 cut(s) 110, 254, 455, 688, 691
TspDTI ATGAA 1 cut(s) 306
TspRI CASTG 1 cut(s) 343
Van91I CCANNNNNTGG 1 cut(s) 28
VpaK11BI GGWCC 2 cut(s) 88, 788
XapI RAATTY 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 124
XmnI GAANNNNTTC 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.