MD01G1217600.v1.1

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012960
Physical Location & Seq
Forward (+)
31017019 .. 31018592
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1217600.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGCGGATTCCATTTTCTCCCAACTTCTGGTTCCAAAAATTGAGACCGACCCACATGATGAGTTTAACTCCTCCATACAGTACTACCAAGACCCATGTCAGGATCAAAATGCTGCGTTAGAAAGTCTACGGCTTGAAAATTTCAATCGGTGTAATTCACCCCCATTGCCGTTGCAATACAATTCCAACCAACTTCCACTAATGGGTTCTCAAGATTTTGAGGATTTAGGCATCTGGGACTATATTTTGGACGATGCTGCTGTTCCATCACCTTTTGATTACGAGGTGGTTGATGAAAATCCCCTGACAACAATGGCGGAATCCATCGTCTGTTCGAACAGTGTTTCGGGTGATGTTTTCAGGATGCTTGATTGGGGGATGGAAGGTTGGGGGAAACACGATGGGGAAGGCATGACAAAAAATGGGAGTTGCTCTAAGAGAATGTCTGCTGCTCCATTGGAATTGGACGAGATTCAGAAGTATTTCGACGTGCCGATAACTCAGGCGGCCAAGGAATTGAAGGTGGGATTAACGGTTTTGAAGAGGAGGTGCAGGGAACTGAATATCATGAGGTGGCCGCACAGAAAGCTCAGGAGCTTGAATGCTCTCATTGAAAATGTTAAGGGGATGGGATTGACGGATGAGCTGAGGATGCTGGAGGAGCACAAGAGGATTCTAGAGCAGCTGCCGGACATGGAGTTGCCGGAGAGAGCCAAGAGGCTGAGGCAAGCTTGCTTCAAAGCCAACTACAAGAAGAAGAGGACTTGGTGTTTGACTGCGGGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

263

Amino Acids

30.05

Weight (kDa)

5.19

Isoelectric Point (pI)

53.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 153 - 200 8.5e-24 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 28
AccI GTMKAC 1 cut(s) 127
AciI CCGC 5 cut(s) 5, 317, 506, 578, 779
AclWI GGATC 1 cut(s) 111
AcoI YGGCCR 2 cut(s) 507, 575
AcsI RAATTY 1 cut(s) 139
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 3 cut(s) 28, 100, 203
AgsI TTSAA 7 cut(s) 137, 145, 520, 541, 601, 614, 739
AhdI GACNNNNNGTC 1 cut(s) 780
AjiI CACGTC 1 cut(s) 490
AjuI GAANNNNNNNTTGG 2 cut(s) 15, 47
AluBI AGCT 5 cut(s) 589, 597, 646, 685, 731
AluI AGCT 5 cut(s) 589, 597, 646, 685, 731
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 1 cut(s) 111
AoxI GGCC 2 cut(s) 507, 575
ApeKI GCWGC 5 cut(s) 113, 257, 449, 682, 685
ApoI RAATTY 1 cut(s) 139
ArsI GACNNNNNNTTYG 2 cut(s) 754, 786
Asp700I GAANNNNTTC 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 782
AsuHPI GGTGA 3 cut(s) 150, 261, 362
AsuII TTCGAA 1 cut(s) 335
AvaII GGWCC 1 cut(s) 782
Bbv12I GWGCWC 1 cut(s) 666
BbvCI CCTCAGC 2 cut(s) 647, 722
BbvI GCAGC 5 cut(s) 100, 244, 436, 672, 694
BccI CCATC 5 cut(s) 274, 332, 373, 395, 622
BceAI ACGGC 2 cut(s) 146, 154
BcoDI GTCTC 1 cut(s) 38
BfaI CTAG 1 cut(s) 677
BisI GCNGC 7 cut(s) 114, 258, 450, 507, 578, 683, 686
BlsI GCNGC 7 cut(s) 115, 259, 451, 508, 579, 684, 687
BmcAI AGTACT 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 782
BmeRI GACNNNNNGTC 1 cut(s) 780
BmgBI CACGTC 1 cut(s) 490
BmgT120I GGNCC 1 cut(s) 782
BmiI GGNNCC 2 cut(s) 33, 783
BmsI GCATC 4 cut(s) 240, 244, 354, 642
BoxI GACNNNNGTC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpmI CTGGAG 1 cut(s) 677
Bpu10I CCTNAGC 3 cut(s) 590, 647, 722
Bpu14I TTCGAA 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 195
BsaBI GATNNNNATC 1 cut(s) 297
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 510
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 100, 203
Bse3DI GCAATG 1 cut(s) 164
Bse8I GATNNNNATC 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 5 cut(s) 369, 384, 633, 646, 657
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 3 cut(s) 28, 100, 203
BseMI GCAATG 1 cut(s) 164
BseMII CTCAG 4 cut(s) 515, 604, 638, 713
BseRI GAGGAG 3 cut(s) 61, 559, 674
BseXI GCAGC 5 cut(s) 100, 244, 436, 672, 694
BsgI GTGCAG 1 cut(s) 571
BshFI GGCC 2 cut(s) 509, 577
BsiHKAI GWGCWC 1 cut(s) 666
BsiSI CCGG 2 cut(s) 689, 704
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 3 cut(s) 28, 100, 203
BsmAI GTCTC 1 cut(s) 38
BsmFI GGGAC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 2 cut(s) 509, 577
Bso31I GGTCTC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 5 cut(s) 5, 317, 506, 578, 779
BspANI GGCC 2 cut(s) 509, 577
BspCNI CTCAG 4 cut(s) 514, 603, 639, 714
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 2 cut(s) 33, 783
BspPI GGATC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 164
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 510
Bst4CI ACNGT 3 cut(s) 81, 341, 535
Bst6I CTCTTC 2 cut(s) 536, 752
BstBI TTCGAA 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 729, 733
BstDEI CTNAG 5 cut(s) 435, 501, 590, 647, 722
BstF5I GGATG 5 cut(s) 369, 384, 633, 646, 657
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 3 cut(s) 586, 652, 661
BstPAI GACNNNNGTC 1 cut(s) 96
BstV1I GCAGC 5 cut(s) 100, 244, 436, 672, 694
BsuRI GGCC 2 cut(s) 509, 577
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 5 cut(s) 369, 384, 633, 646, 657
BtsIMutI CAGTG 1 cut(s) 346
Cac8I GCNNGC 2 cut(s) 729, 733
CciI TCATGA 1 cut(s) 567
Cfr13I GGNCC 1 cut(s) 782
Csp6I GTAC 1 cut(s) 82
CviAII CATG 6 cut(s) 56, 96, 412, 568, 694, 786
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 5 cut(s) 435, 501, 590, 647, 722
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
DriI GACNNNNNGTC 1 cut(s) 780
EaeI YGGCCR 2 cut(s) 507, 575
Eam1104I CTCTTC 2 cut(s) 536, 752
Eam1105I GACNNNNNGTC 1 cut(s) 780
EarI CTCTTC 2 cut(s) 536, 752
EciI GGCGGA 2 cut(s) 20, 332
Eco130I CCWWGG 1 cut(s) 510
Eco31I GGTCTC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 782
EcoT14I CCWWGG 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 6 cut(s) 59, 99, 415, 571, 697, 789
FaiI YATR 8 cut(s) 57, 77, 97, 243, 413, 569, 695, 787
FaqI GGGAC 1 cut(s) 251
FatI CATG 6 cut(s) 55, 95, 411, 567, 693, 785
FauI CCCGC 1 cut(s) 772
FblI GTMKAC 1 cut(s) 127
Fnu4HI GCNGC 7 cut(s) 114, 258, 450, 507, 578, 683, 686
FokI GGATG 5 cut(s) 376, 391, 640, 653, 664
Fsp4HI GCNGC 7 cut(s) 114, 258, 450, 507, 578, 683, 686
FspBI CTAG 1 cut(s) 677
GluI GCNGC 7 cut(s) 114, 258, 450, 507, 578, 683, 686
GsuI CTGGAG 1 cut(s) 677
HaeIII GGCC 2 cut(s) 509, 577
HapII CCGG 2 cut(s) 689, 704
Hin1II CATG 6 cut(s) 59, 99, 415, 571, 697, 789
HindIII AAGCTT 1 cut(s) 729
HinfI GANTC 4 cut(s) 8, 320, 472, 673
HpaII CCGG 2 cut(s) 689, 704
HphI GGTGA 3 cut(s) 150, 261, 362
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 477
Hpy188III TCNNGA 6 cut(s) 101, 212, 361, 568, 592, 677
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 3 cut(s) 377, 401, 514
HpyCH4III ACNGT 3 cut(s) 81, 341, 535
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 2 cut(s) 175, 552
HpyF10VI GCNNNNNNNGC 3 cut(s) 586, 652, 661
HpyF3I CTNAG 5 cut(s) 435, 501, 590, 647, 722
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 6 cut(s) 59, 99, 415, 571, 697, 789
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 3 cut(s) 457, 594, 661
Lsp1109I GCAGC 5 cut(s) 100, 244, 436, 672, 694
LweI GCATC 4 cut(s) 240, 244, 354, 642
MaeI CTAG 1 cut(s) 677
MaeII ACGT 1 cut(s) 489
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 3 cut(s) 553, 766, 769
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 6 cut(s) 39, 139, 154, 181, 461, 515
MmeI TCCRAC 1 cut(s) 210
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 3 cut(s) 66, 530, 621
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 2 cut(s) 689, 704
Mva1269I GAATGC 1 cut(s) 607
MwoI GCNNNNNNNGC 3 cut(s) 586, 652, 661
NdeII GATC 1 cut(s) 103
NlaIII CATG 6 cut(s) 59, 99, 415, 571, 697, 789
NlaIV GGNNCC 2 cut(s) 33, 783
NspV TTCGAA 1 cut(s) 335
PagI TCATGA 1 cut(s) 567
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 4 cut(s) 8, 320, 472, 673
PflMI CCANNNNNTGG 1 cut(s) 28
PkrI GCNGC 7 cut(s) 115, 259, 451, 508, 579, 684, 687
PshAI GACNNNNGTC 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 33, 783
PspPI GGNCC 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 685
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SaqAI TTAA 3 cut(s) 66, 530, 621
SatI GCNGC 7 cut(s) 114, 258, 450, 507, 578, 683, 686
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 1 cut(s) 782
ScaI AGTACT 1 cut(s) 83
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 4 cut(s) 240, 244, 354, 642
SfuI TTCGAA 1 cut(s) 335
SinI GGWCC 1 cut(s) 782
SmlI CTYRAG 1 cut(s) 210
SmoI CTYRAG 1 cut(s) 210
Sse9I AATT 6 cut(s) 39, 139, 154, 181, 461, 515
SsiI CCGC 5 cut(s) 5, 317, 506, 578, 779
SspMI CTAG 1 cut(s) 677
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 3 cut(s) 81, 341, 535
TaiI ACGT 1 cut(s) 492
TaqI TCGA 2 cut(s) 335, 486
TaqII GACCGA 1 cut(s) 62
TasI AATT 6 cut(s) 39, 139, 154, 181, 461, 515
TatI WGTACW 1 cut(s) 81
TauI GCSGC 2 cut(s) 509, 580
TfiI GAWTC 4 cut(s) 8, 320, 472, 673
Tru1I TTAA 3 cut(s) 66, 530, 621
Tru9I TTAA 3 cut(s) 66, 530, 621
TscAI CASTG 1 cut(s) 346
TseI GCWGC 5 cut(s) 113, 257, 449, 682, 685
TspDTI ATGAA 1 cut(s) 309
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 1 cut(s) 346
Van91I CCANNNNNTGG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 782
XapI RAATTY 1 cut(s) 139
XbaI TCTAGA 1 cut(s) 676
XmiI GTMKAC 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 1 cut(s) 677
ZrmI AGTACT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.