Rh1AG445300

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0012960
Physical Location & Seq
Forward (+)
66549773 .. 66551565
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG445300.1

Sequence Viewer

Length: 1254 bp
ATGGAGTATTCTACTCAACAACTAGTTATTCCAAAGATTGAAGAAAACCCACTTGAATTTGACTACTTAGAGTACTACTATCAAGACCCATTTCAAAATAGTACTACTTCGTTATTTGAAGCTCCACTGCTTGAAGATTTCCATGACCATGAATTCAATTCACTAGCTTTGCCATTATCAGAAGATTACAACCAAATTCCCCACATGGGTTTTCAAGGACTTGAAGATTTCAGCAACTGGGACTTTACTGCTTTGGACATCACCACTGCTGTTTCATCACCATCTCATTGTGATAATCATGTGCTTGATGAAAAGCCTCTTCCAACAATGGTGGATTCTTTTGACTACTTGGACAATGTCCCAAGTGATTCTGGTCATGGTGAAGTTTCATTCAAGATTCAAAGATCTGGTTTTTCATCATCTCATTGTGATTCTTTGGTGTTTGATGAAAAGCCTCTTACAACAATGGTGGAGTCCTTTACCTACTTGGACAATGTCCTAAGTGATTTGGGTCAGCATGAAGTTTCAATGATGACTGAAAGCTGTGGCTTTTCATCATCTCATTGTGATTCTGTGGTGTTCAATGAAAAGCCCATTTCAACAATGGTGGAGTCATTTGACAACTCAAACAATGTCCCGAGTGTTTTGGGTCCCCATGAAGCTTCCATGAGGACTGAAAGCGCTACTGCTTTTGCGTCGTCCCATAGTAATTCTCTGGTGCTTTATGAAAAACCCCTTTCAACAATTGTGAATTCTTTCGACTACTCTGATATTCCTACACCTGATTTGGGCCATGATGAAGTTTCAGTGATGATTGAAAATGGGTTTCCTAGTTGGGACATATCAGACATTAGTTGTAAAAGAGAGACAAGTAATAGAAGGAGATATAATACAGAGATGAAGATCGAGTCTGATTCTTTGGAATTTGAGGAGATAAAGAAGTACTTTGACCTCCCCATAGCAAAAGCAGCCAAGGAACTGAAGGTGGGAGTGACTCTGTTGAAGAGGAGGTGCAGGGAGCTCAACATCATGAGGTGGCCTCACAGAAAGATTAGGAGCTTGAACTCTCTCATTGAAACTCTCAAGGGAAAGGGTTTGAGAAATGAGGTAAAGATGCTGGAGGAGAACAAAAGGCTTCTAGAGCAAGTGCCGAACATGGAGCTAACAAAGAGAACCAAAAAGCTGAGGCAGTCTATTTTCAAAGCCAACTACAACTACAAAAAGCAGATGTCTTTGTCTTTGTTGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

417

Amino Acids

47.73

Weight (kDa)

4.95

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 306 - 354 1.3e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 5 cut(s) 56, 152, 195, 751, 923
AcuI CTGAAG 1 cut(s) 1001
AfaI GTAC 3 cut(s) 74, 103, 944
AfeI AGCGCT 1 cut(s) 682
AfiI CCNNNNNNNGG 2 cut(s) 206, 788
AhlI ACTAGT 1 cut(s) 22
AluBI AGCT 8 cut(s) 122, 167, 543, 662, 1021, 1059, 1162, 1183
AluI AGCT 8 cut(s) 122, 167, 543, 662, 1021, 1059, 1162, 1183
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 1 cut(s) 860
AlwNI CAGNNNCTG 1 cut(s) 237
Ama87I CYCGRG 1 cut(s) 637
Aor51HI AGCGCT 1 cut(s) 682
AoxI GGCC 2 cut(s) 790, 1037
ApeKI GCWGC 1 cut(s) 968
ApoI RAATTY 5 cut(s) 56, 152, 195, 751, 923
Asp700I GAANNNNTTC 1 cut(s) 755
AspLEI GCGC 1 cut(s) 683
AspS9I GGNCC 2 cut(s) 650, 790
AsuHPI GGTGA 3 cut(s) 253, 270, 392
AvaI CYCGRG 1 cut(s) 637
AvaII GGWCC 1 cut(s) 650
BanII GRGCYC 1 cut(s) 1023
Bbv12I GWGCWC 1 cut(s) 1023
BbvCI CCTCAGC 1 cut(s) 1184
BbvI GCAGC 1 cut(s) 980
BccI CCATC 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 860
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 4 cut(s) 23, 164, 831, 1139
BfoI RGCGCY 1 cut(s) 684
BglII AGATCT 1 cut(s) 404
BisI GCNGC 1 cut(s) 969
BlsI GCNGC 1 cut(s) 970
BmcAI AGTACT 3 cut(s) 74, 103, 944
Bme18I GGWCC 1 cut(s) 650
BmeT110I CYCGRG 1 cut(s) 637
BmgT120I GGNCC 2 cut(s) 650, 790
BmiI GGNNCC 2 cut(s) 651, 652
BmrI ACTGGG 1 cut(s) 247
BmsI GCATC 1 cut(s) 1104
BmuI ACTGGG 1 cut(s) 247
BplI GAGNNNNNCTC 2 cut(s) 1024, 1056
BpmI CTGGAG 1 cut(s) 1139
Bpu10I CCTNAGC 1 cut(s) 1184
BpuEI CTTGAG 1 cut(s) 1067
BsaBI GATNNNNATC 1 cut(s) 902
BsaJI CCNNGG 1 cut(s) 972
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 788
Bse1I ACTGG 1 cut(s) 242
Bse8I GATNNNNATC 1 cut(s) 902
BseDI CCNNGG 1 cut(s) 972
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 2 cut(s) 206, 788
BseMII CTCAG 1 cut(s) 1175
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 3 cut(s) 944, 1021, 1136
BseXI GCAGC 1 cut(s) 980
BsgI GTGCAG 1 cut(s) 1033
BshFI GGCC 2 cut(s) 792, 1039
BsiHKAI GWGCWC 1 cut(s) 1023
BsiHKCI CYCGRG 1 cut(s) 637
BslFI GGGAC 6 cut(s) 254, 344, 620, 636, 685, 851
BslI CCNNNNNNNGG 2 cut(s) 206, 788
BsmAI GTCTC 1 cut(s) 860
BsmFI GGGAC 6 cut(s) 254, 344, 620, 636, 685, 851
BsnI GGCC 2 cut(s) 792, 1039
BsoBI CYCGRG 1 cut(s) 637
Bsp1286I GDGCHC 1 cut(s) 1023
Bsp143I GATC 2 cut(s) 404, 903
BspANI GGCC 2 cut(s) 792, 1039
BspCNI CTCAG 1 cut(s) 1176
BspHI TCATGA 1 cut(s) 1029
BspLI GGNNCC 2 cut(s) 651, 652
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 972
BssMI GATC 2 cut(s) 404, 903
BssT1I CCWWGG 1 cut(s) 972
Bst6I CTCTTC 2 cut(s) 324, 998
BstDEI CTNAG 3 cut(s) 67, 500, 1184
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 1 cut(s) 683
BstKTI GATC 2 cut(s) 407, 906
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 2 cut(s) 404, 903
BstMWI GCNNNNNNNGC 2 cut(s) 968, 1141
BstV1I GCAGC 1 cut(s) 980
BstX2I RGATCY 1 cut(s) 404
BstYI RGATCY 1 cut(s) 404
BsuRI GGCC 2 cut(s) 792, 1039
BtsI GCAGTG 2 cut(s) 125, 264
BtsIMutI CAGTG 3 cut(s) 125, 264, 813
CaiI CAGNNNCTG 1 cut(s) 237
CciI TCATGA 1 cut(s) 1029
CfoI GCGC 1 cut(s) 683
Cfr13I GGNCC 2 cut(s) 650, 790
CseI GACGC 1 cut(s) 684
Csp6I GTAC 3 cut(s) 73, 102, 943
CspCI CAANNNNNGTGG 6 cut(s) 312, 347, 450, 485, 588, 623
CviQI GTAC 3 cut(s) 73, 102, 943
DdeI CTNAG 3 cut(s) 67, 500, 1184
DpnI GATC 2 cut(s) 406, 905
DpnII GATC 2 cut(s) 404, 903
Eam1104I CTCTTC 2 cut(s) 324, 998
EarI CTCTTC 2 cut(s) 324, 998
Ecl136II GAGCTC 1 cut(s) 1021
Eco130I CCWWGG 1 cut(s) 972
Eco24I GRGCYC 1 cut(s) 1023
Eco47I GGWCC 1 cut(s) 650
Eco47III AGCGCT 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 1021
Eco57I CTGAAG 1 cut(s) 1001
Eco88I CYCGRG 1 cut(s) 637
EcoICRI GAGCTC 1 cut(s) 1021
EcoO109I RGGNCCY 1 cut(s) 650
EcoRI GAATTC 2 cut(s) 152, 751
EcoT14I CCWWGG 1 cut(s) 972
EcoT38I GRGCYC 1 cut(s) 1023
ErhI CCWWGG 1 cut(s) 972
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 6 cut(s) 254, 344, 620, 636, 685, 851
Fnu4HI GCNGC 1 cut(s) 969
FriOI GRGCYC 1 cut(s) 1023
Fsp4HI GCNGC 1 cut(s) 969
FspBI CTAG 4 cut(s) 23, 164, 831, 1139
GlaI GCGC 1 cut(s) 682
GluI GCNGC 1 cut(s) 969
GsuI CTGGAG 1 cut(s) 1139
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 2 cut(s) 792, 1039
HgaI GACGC 1 cut(s) 684
HhaI GCGC 1 cut(s) 683
Hin6I GCGC 1 cut(s) 681
HinP1I GCGC 1 cut(s) 681
HindIII AAGCTT 1 cut(s) 660
HphI GGTGA 3 cut(s) 253, 270, 392
Hpy188I TCNGA 4 cut(s) 181, 769, 847, 913
Hpy188III TCNNGA 5 cut(s) 83, 394, 637, 1030, 1139
Hpy99I CGWCG 1 cut(s) 700
HpyAV CCTTC 2 cut(s) 873, 976
HpyCH4V TGCA 1 cut(s) 1014
HpyF10VI GCNNNNNNNGC 2 cut(s) 968, 1141
HpyF3I CTNAG 3 cut(s) 67, 500, 1184
HspAI GCGC 1 cut(s) 681
KflI GGGWCCC 1 cut(s) 650
Kzo9I GATC 2 cut(s) 404, 903
LmnI GCTCC 4 cut(s) 127, 1018, 1056, 1159
LpnPI CCDG 7 cut(s) 223, 357, 393, 701, 795, 1000, 1103
Lsp1109I GCAGC 1 cut(s) 980
LweI GCATC 1 cut(s) 1104
MaeI CTAG 4 cut(s) 23, 164, 831, 1139
MaeIII GTNAC 1 cut(s) 991
MalI GATC 2 cut(s) 406, 905
MboI GATC 2 cut(s) 404, 903
MboII GAAGA 7 cut(s) 53, 146, 194, 236, 311, 913, 1015
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 1 cut(s) 404
MhlI GDGCHC 1 cut(s) 1023
MluCI AATT 8 cut(s) 56, 152, 157, 195, 709, 744, 751, 923
MlyI GAGTC 4 cut(s) 482, 620, 917, 988
MmeI TCCRAC 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 755
MslI CAYNNNNRTG 1 cut(s) 147
MunI CAATTG 1 cut(s) 744
MwoI GCNNNNNNNGC 2 cut(s) 968, 1141
NdeII GATC 2 cut(s) 404, 903
NlaIV GGNNCC 2 cut(s) 651, 652
NmuCI GTSAC 1 cut(s) 991
PagI TCATGA 1 cut(s) 1029
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 6 cut(s) 335, 368, 397, 431, 569, 914
PflFI GACNNNGTC 2 cut(s) 356, 494
PkrI GCNGC 1 cut(s) 970
PleI GAGTC 4 cut(s) 481, 619, 916, 988
PpsI GAGTC 4 cut(s) 481, 619, 916, 988
PpuMI RGGWCCY 1 cut(s) 650
Psp124BI GAGCTC 1 cut(s) 1023
Psp5II RGGWCCY 1 cut(s) 650
PspN4I GGNNCC 2 cut(s) 651, 652
PspPI GGNCC 2 cut(s) 650, 790
PspPPI RGGWCCY 1 cut(s) 650
PstNI CAGNNNCTG 1 cut(s) 237
PsuI RGATCY 1 cut(s) 404
PsyI GACNNNGTC 2 cut(s) 356, 494
RsaI GTAC 3 cut(s) 74, 103, 944
RsaNI GTAC 3 cut(s) 73, 102, 943
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 1023
SatI GCNGC 1 cut(s) 969
Sau3AI GATC 2 cut(s) 404, 903
Sau96I GGNCC 2 cut(s) 650, 790
ScaI AGTACT 3 cut(s) 74, 103, 944
SchI GAGTC 4 cut(s) 482, 620, 917, 988
SduI GDGCHC 1 cut(s) 1023
SfaNI GCATC 1 cut(s) 1104
SinI GGWCC 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 1082
SmoI CTYRAG 1 cut(s) 1082
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 8 cut(s) 56, 152, 157, 195, 709, 744, 751, 923
SspMI CTAG 4 cut(s) 23, 164, 831, 1139
SstI GAGCTC 1 cut(s) 1023
StyI CCWWGG 1 cut(s) 972
TaqI TCGA 2 cut(s) 759, 906
TasI AATT 8 cut(s) 56, 152, 157, 195, 709, 744, 751, 923
TatI WGTACW 3 cut(s) 72, 101, 942
TfiI GAWTC 6 cut(s) 335, 368, 397, 431, 569, 914
TscAI CASTG 3 cut(s) 132, 271, 813
TseFI GTSAC 1 cut(s) 991
TseI GCWGC 1 cut(s) 968
Tsp45I GTSAC 1 cut(s) 991
TspRI CASTG 3 cut(s) 132, 271, 813
Tth111I GACNNNGTC 2 cut(s) 356, 494
VpaK11BI GGWCC 1 cut(s) 650
XapI RAATTY 5 cut(s) 56, 152, 195, 751, 923
XbaI TCTAGA 1 cut(s) 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 4 cut(s) 23, 164, 831, 1139
ZrmI AGTACT 3 cut(s) 74, 103, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.