MD07G1286800.v1.1

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012960
Physical Location & Seq
Forward (+)
34857497 .. 34858777
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1286800.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGGTTCAAAAGATTTTGAGGATTCAGGCATCTGGGACTACATTTTGGACGACGCTGCTTTTCCATCAACTTTTGGTTACGAGGTGGTTGATGAAAAGCCCCTGACAACAATGGCGGAATCCGTTGACTGTTCGAACAGTGTTTCGGGTGATTCGGACGATGTTTTGAGGACGATCGAGTGGGGGATGAAGATTTGGGGCAAAGATGAAGAGGGAAGCAGGACAAAAAATGAGAGTTTTTGCAAGAAGAGGTGTGCCACTCCATTGGAACTGGACGAGATTCAGAAGTATTTCGACGTGCCGATAACTCAAGCGGCCAAGGAGTTGAAGGTGGGATTGACGGTTTTGAAGAGGAGGTGCAGGGAACTGAACATCATGAGGTGGCCGCACAGAAAGATCAAGAGCTTGAATGCTCTCATTGAAAATGTTAAGGGGATGGGATTGACGAATGATGTGATGATGCTGGAGGAGCACAGGAGGCTTCTAGAGCAGATGCCGGACAAGGAGTTGCCGGAGAGAGCCAAGAGGCTGAGGCAGGCTTGCTTCAAAACCAACTACAAGAAGAAGAGGGCTTTGTGTTTGACTGCTCTGCCGGTCCATGATTTAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

204

Amino Acids

23.39

Weight (kDa)

6.84

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 89 - 136 2e-24 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 116, 314, 386
AcoI YGGCCR 2 cut(s) 315, 383
AgsI TTSAA 6 cut(s) 9, 328, 349, 409, 422, 547
AjiI CACGTC 1 cut(s) 298
AluBI AGCT 1 cut(s) 405
AluI AGCT 1 cut(s) 405
Alw21I GWGCWC 1 cut(s) 474
AoxI GGCC 2 cut(s) 315, 383
ApeKI GCWGC 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 595
AsuHPI GGTGA 1 cut(s) 161
AsuII TTCGAA 1 cut(s) 134
AvaII GGWCC 1 cut(s) 595
Bbv12I GWGCWC 1 cut(s) 474
BbvCI CCTCAGC 1 cut(s) 530
BbvI GCAGC 1 cut(s) 43
BccI CCATC 2 cut(s) 73, 430
BfaI CTAG 2 cut(s) 485, 610
BisI GCNGC 3 cut(s) 57, 315, 386
BlsI GCNGC 3 cut(s) 58, 316, 387
Bme18I GGWCC 1 cut(s) 595
BmgBI CACGTC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 595
BmsI GCATC 3 cut(s) 39, 450, 483
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 530
Bpu14I TTCGAA 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 318
Bse118I RCCGGY 1 cut(s) 592
Bse1I ACTGG 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 2 cut(s) 192, 441
BseMII CTCAG 1 cut(s) 521
BseNI ACTGG 1 cut(s) 276
BseRI GAGGAG 2 cut(s) 367, 482
BseXI GCAGC 1 cut(s) 43
BsgI GTGCAG 1 cut(s) 379
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 2 cut(s) 317, 385
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 3 cut(s) 497, 512, 593
BslFI GGGAC 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 2 cut(s) 317, 385
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 2 cut(s) 174, 396
BspACI CCGC 3 cut(s) 116, 314, 386
BspANI GGCC 2 cut(s) 317, 385
BspCNI CTCAG 1 cut(s) 522
BspHI TCATGA 1 cut(s) 375
BspT104I TTCGAA 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 592
BsrI ACTGG 1 cut(s) 276
BssAI RCCGGY 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 2 cut(s) 174, 396
BssT1I CCWWGG 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 131, 140, 343
Bst6I CTCTTC 4 cut(s) 204, 242, 344, 560
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 537, 541
BstDEI CTNAG 1 cut(s) 530
BstF5I GGATG 2 cut(s) 192, 441
BstKTI GATC 2 cut(s) 177, 399
BstMBI GATC 2 cut(s) 174, 396
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 3 cut(s) 469, 478, 487
BstV1I GCAGC 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 265
BsuRI GGCC 2 cut(s) 317, 385
BtrI CACGTC 1 cut(s) 298
BtsCI GGATG 2 cut(s) 192, 441
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 537, 541
CciI TCATGA 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 592
Cfr13I GGNCC 1 cut(s) 595
CseI GACGC 1 cut(s) 62
CviAII CATG 2 cut(s) 376, 599
CviJI RGCY 9 cut(s) 100, 317, 385, 405, 481, 521, 529, 539, 572
CviKI_1 RGCY 9 cut(s) 100, 317, 385, 405, 481, 521, 529, 539, 572
DdeI CTNAG 1 cut(s) 530
DpnI GATC 2 cut(s) 176, 398
DpnII GATC 2 cut(s) 174, 396
EaeI YGGCCR 2 cut(s) 315, 383
Eam1104I CTCTTC 4 cut(s) 204, 242, 344, 560
EarI CTCTTC 4 cut(s) 204, 242, 344, 560
EciI GGCGGA 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 2 cut(s) 379, 602
FaiI YATR 2 cut(s) 377, 600
FaqI GGGAC 1 cut(s) 50
FatI CATG 2 cut(s) 375, 598
Fnu4HI GCNGC 3 cut(s) 57, 315, 386
FokI GGATG 2 cut(s) 199, 448
Fsp4HI GCNGC 3 cut(s) 57, 315, 386
FspBI CTAG 2 cut(s) 485, 610
GluI GCNGC 3 cut(s) 57, 315, 386
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 2 cut(s) 317, 385
HapII CCGG 3 cut(s) 497, 512, 593
HgaI GACGC 1 cut(s) 62
Hin1II CATG 2 cut(s) 379, 602
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 4 cut(s) 23, 119, 152, 280
HpaII CCGG 3 cut(s) 497, 512, 593
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 157, 285
Hpy188III TCNNGA 3 cut(s) 376, 400, 485
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 2 cut(s) 56, 299
HpyAV CCTTC 1 cut(s) 322
HpyCH4III ACNGT 3 cut(s) 131, 140, 343
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 2 cut(s) 243, 360
HpyF10VI GCNNNNNNNGC 3 cut(s) 469, 478, 487
HpyF3I CTNAG 1 cut(s) 530
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 2 cut(s) 379, 602
Kzo9I GATC 2 cut(s) 174, 396
LmnI GCTCC 1 cut(s) 469
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 3 cut(s) 39, 450, 483
MaeI CTAG 2 cut(s) 485, 610
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 77
MalI GATC 2 cut(s) 176, 398
MboI GATC 2 cut(s) 174, 396
MboII GAAGA 6 cut(s) 202, 221, 259, 361, 574, 577
MhlI GDGCHC 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 1 cut(s) 429
MspI CCGG 3 cut(s) 497, 512, 593
Mva1269I GAATGC 1 cut(s) 415
MwoI GCNNNNNNNGC 3 cut(s) 469, 478, 487
NdeII GATC 2 cut(s) 174, 396
NlaIII CATG 2 cut(s) 379, 602
NspV TTCGAA 1 cut(s) 134
PagI TCATGA 1 cut(s) 375
PctI GAATGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 4 cut(s) 23, 119, 152, 280
PkrI GCNGC 3 cut(s) 58, 316, 387
Ple19I CGATCG 1 cut(s) 177
PspPI GGNCC 1 cut(s) 595
PvuI CGATCG 1 cut(s) 177
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 3 cut(s) 57, 315, 386
Sau3AI GATC 2 cut(s) 174, 396
Sau96I GGNCC 1 cut(s) 595
SduI GDGCHC 1 cut(s) 474
SetI ASST 7 cut(s) 87, 254, 300, 333, 359, 383, 407
SfaNI GCATC 3 cut(s) 39, 450, 483
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 1 cut(s) 595
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
SsiI CCGC 3 cut(s) 116, 314, 386
SspMI CTAG 2 cut(s) 485, 610
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 3 cut(s) 131, 140, 343
TaiI ACGT 1 cut(s) 300
TaqI TCGA 3 cut(s) 134, 177, 294
TauI GCSGC 2 cut(s) 317, 388
TfiI GAWTC 4 cut(s) 23, 119, 152, 280
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 1 cut(s) 145
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 3 cut(s) 108, 203, 222
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 595
XbaI TCTAGA 1 cut(s) 484
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 2 cut(s) 485, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.