Rw1G038750

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012960
Physical Location & Seq
Forward (+)
66284604 .. 66286388
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G038750.1

Sequence Viewer

Length: 1221 bp
ATGGAGTACTCTACCCAACAACTTGTTATTCCAAAGATTGAAGAAAACCCACTTGAATTTGACTACTTAGAGTACTACTATCAAGACCCATTTCAAATTAGTACTACTTCGTTATTTGAAGCTCCACTGCTTGAAGATTTCCATGACCATGAATTCAATTCACTAGCTTTGCCATTATCAGAAGATTACAACCAAATTCCCCGCATGGGTTTTCAAGGACTTGAAGATTTCAGCACCTGGGACTTTACTGCTTTGGACATCACCACTGCTGTTTCATCACCATCTCATTGTGATAATCATGTGCTTGATGAAAAGCCTCTTCCAACAATGGTGGATTCTTTGGCTACTTGGACAATGTCCCAAATTGAAACATCTGGTTTTTCATCATCTCATTGTGATTCTTTGGTGTTTGATGAAAAGCCTCTTACAACAATGGTGGAGTCCTTTACCTACTTGGACAATGTCCCAAGTGATTTGGGTCAGCATGAAGTTTCAATGATGACTGAAAGCTGTGGCTTTTCATCATCTCATTGTGATTCTGTGGTGTTCAATGAAAAGCCCATTTCAACAATGGTAGAGTCATTTGACAACTCAAACAATGTCCCGAGTGTTCTGGGTCCCCATGAAGCTTCCATGAGGACTGAAAGCGCTACTGCTTTTGCGTCGTCCCATAGTAATTCTCTGGTGCTTTATGAAATACCCCTTTCAACAATCGTGGATTCTTTCGACTACTCTGATATTCCTACACCTGATTTGGGCCATGATGAAGTTTCAGTGATGATTGAAAATGGGTTTCCTAGTTGGGACATATCAGACATTAGTTGTAAAAGAGAGACAAGTAATAGAAGGAGATATAATACAGAGATGAAGATCAAGTCTGATTCTTTGGAATTTGAGGAGATAAAGAAGTACTTTGACCTCCCCATAACAAAAGCAGCCAAGGAACTGAAGGTGGGAGTGACTCTGTTGAAGAGGAGGTGCAGGGAGCTCAACATCATGAGGTGGCCTCACAGAAAGATTAGGAGCTTGAACTCTCTCATTGAAACTCTCAAGGGAATGGGTTTGAGAAATGAGGTAAAGATGCTGGAGGAGAACAAAAGGCTTCTAGAGCAAGTGCCAAACATGGAGCTAACAAAGAGAACCAAAAAGCTGAGGCAGTCTATTTTCAAAGCTAACTACAACTACAAAAAGCAGATGTCTTTGTCTTTGTTGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

406

Amino Acids

46.5

Weight (kDa)

4.89

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 295 - 343 1.1e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 202
AcsI RAATTY 4 cut(s) 56, 152, 195, 890
AcuI CTGAAG 1 cut(s) 968
AfaI GTAC 4 cut(s) 8, 74, 103, 911
AfeI AGCGCT 1 cut(s) 649
AfiI CCNNNNNNNGG 2 cut(s) 206, 755
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 9 cut(s) 122, 167, 510, 629, 988, 1026, 1129, 1150, 1172
AluI AGCT 9 cut(s) 122, 167, 510, 629, 988, 1026, 1129, 1150, 1172
Alw21I GWGCWC 1 cut(s) 990
Alw26I GTCTC 1 cut(s) 827
AlwNI CAGNNNCTG 1 cut(s) 237
Ama87I CYCGRG 1 cut(s) 604
Aor51HI AGCGCT 1 cut(s) 649
AoxI GGCC 2 cut(s) 757, 1004
ApeKI GCWGC 1 cut(s) 935
ApoI RAATTY 4 cut(s) 56, 152, 195, 890
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 2 cut(s) 617, 757
AsuHPI GGTGA 2 cut(s) 253, 270
AvaI CYCGRG 1 cut(s) 604
AvaII GGWCC 1 cut(s) 617
BanII GRGCYC 1 cut(s) 990
Bbv12I GWGCWC 1 cut(s) 990
BbvCI CCTCAGC 1 cut(s) 1151
BbvI GCAGC 1 cut(s) 947
BccI CCATC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 827
BfaI CTAG 3 cut(s) 164, 798, 1106
BfoI RGCGCY 1 cut(s) 651
BisI GCNGC 1 cut(s) 936
BlsI GCNGC 1 cut(s) 937
BmcAI AGTACT 4 cut(s) 8, 74, 103, 911
Bme1390I CCNGG 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 604
BmgT120I GGNCC 2 cut(s) 617, 757
BmiI GGNNCC 2 cut(s) 618, 619
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 1071
BplI GAGNNNNNCTC 2 cut(s) 991, 1023
BpmI CTGGAG 1 cut(s) 1106
Bpu10I CCTNAGC 1 cut(s) 1151
BpuEI CTTGAG 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 869
BsaJI CCNNGG 2 cut(s) 237, 939
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 755
Bse8I GATNNNNATC 1 cut(s) 869
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 2 cut(s) 237, 939
BseJI GATNNNNATC 1 cut(s) 869
BseLI CCNNNNNNNGG 2 cut(s) 206, 755
BseMII CTCAG 1 cut(s) 1142
BseRI GAGGAG 3 cut(s) 911, 988, 1103
BseXI GCAGC 1 cut(s) 947
BsgI GTGCAG 1 cut(s) 1000
BshFI GGCC 2 cut(s) 759, 1006
BsiHKAI GWGCWC 1 cut(s) 990
BsiHKCI CYCGRG 1 cut(s) 604
BslFI GGGAC 7 cut(s) 254, 343, 449, 587, 603, 652, 818
BslI CCNNNNNNNGG 2 cut(s) 206, 755
BsmAI GTCTC 1 cut(s) 827
BsmFI GGGAC 7 cut(s) 254, 343, 449, 587, 603, 652, 818
BsnI GGCC 2 cut(s) 759, 1006
BsoBI CYCGRG 1 cut(s) 604
Bsp1286I GDGCHC 1 cut(s) 990
Bsp143I GATC 1 cut(s) 870
BspACI CCGC 1 cut(s) 202
BspANI GGCC 2 cut(s) 759, 1006
BspCNI CTCAG 1 cut(s) 1143
BspHI TCATGA 1 cut(s) 996
BspLI GGNNCC 2 cut(s) 618, 619
BssECI CCNNGG 2 cut(s) 237, 939
BssMI GATC 1 cut(s) 870
BssT1I CCWWGG 1 cut(s) 939
Bst2UI CCWGG 1 cut(s) 238
Bst6I CTCTTC 2 cut(s) 324, 965
BstDEI CTNAG 2 cut(s) 67, 1151
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 1 cut(s) 873
BstMAI GTCTC 1 cut(s) 827
BstMBI GATC 1 cut(s) 870
BstMWI GCNNNNNNNGC 1 cut(s) 1108
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 947
BsuRI GGCC 2 cut(s) 759, 1006
BtsI GCAGTG 2 cut(s) 125, 264
BtsIMutI CAGTG 3 cut(s) 125, 264, 780
CaiI CAGNNNCTG 1 cut(s) 237
CciI TCATGA 1 cut(s) 996
CfoI GCGC 1 cut(s) 650
Cfr13I GGNCC 2 cut(s) 617, 757
CseI GACGC 1 cut(s) 651
Csp6I GTAC 4 cut(s) 7, 73, 102, 910
CspCI CAANNNNNGTGG 6 cut(s) 312, 347, 417, 452, 696, 731
CviQI GTAC 4 cut(s) 7, 73, 102, 910
DdeI CTNAG 2 cut(s) 67, 1151
DpnI GATC 1 cut(s) 872
DpnII GATC 1 cut(s) 870
Eam1104I CTCTTC 2 cut(s) 324, 965
EarI CTCTTC 2 cut(s) 324, 965
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 1 cut(s) 939
Eco24I GRGCYC 1 cut(s) 990
Eco47I GGWCC 1 cut(s) 617
Eco47III AGCGCT 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 1 cut(s) 968
Eco88I CYCGRG 1 cut(s) 604
EcoICRI GAGCTC 1 cut(s) 988
EcoO109I RGGNCCY 1 cut(s) 617
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 939
EcoT38I GRGCYC 1 cut(s) 990
ErhI CCWWGG 1 cut(s) 939
FalI AAGNNNNNCTT 2 cut(s) 896, 928
FaqI GGGAC 7 cut(s) 254, 343, 449, 587, 603, 652, 818
FauI CCCGC 1 cut(s) 209
Fnu4HI GCNGC 1 cut(s) 936
FriOI GRGCYC 1 cut(s) 990
Fsp4HI GCNGC 1 cut(s) 936
FspBI CTAG 3 cut(s) 164, 798, 1106
GlaI GCGC 1 cut(s) 649
GluI GCNGC 1 cut(s) 936
GsuI CTGGAG 1 cut(s) 1106
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 2 cut(s) 759, 1006
HgaI GACGC 1 cut(s) 651
HhaI GCGC 1 cut(s) 650
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HindIII AAGCTT 1 cut(s) 627
HinfI GANTC 8 cut(s) 335, 398, 440, 536, 578, 719, 881, 961
HphI GGTGA 2 cut(s) 253, 270
Hpy188I TCNGA 4 cut(s) 181, 736, 814, 880
Hpy188III TCNNGA 4 cut(s) 83, 604, 997, 1106
Hpy99I CGWCG 1 cut(s) 667
HpyAV CCTTC 2 cut(s) 840, 943
HpyCH4V TGCA 1 cut(s) 981
HpyF10VI GCNNNNNNNGC 1 cut(s) 1108
HpyF3I CTNAG 2 cut(s) 67, 1151
HspAI GCGC 1 cut(s) 648
KflI GGGWCCC 1 cut(s) 617
Kzo9I GATC 1 cut(s) 870
LmnI GCTCC 4 cut(s) 127, 985, 1023, 1126
LpnPI CCDG 8 cut(s) 223, 250, 360, 599, 668, 762, 967, 1070
Lsp1109I GCAGC 1 cut(s) 947
LweI GCATC 1 cut(s) 1071
MaeI CTAG 3 cut(s) 164, 798, 1106
MaeIII GTNAC 1 cut(s) 958
MalI GATC 1 cut(s) 872
MboI GATC 1 cut(s) 870
MboII GAAGA 7 cut(s) 53, 146, 194, 236, 311, 880, 982
MhlI GDGCHC 1 cut(s) 990
MluCI AATT 8 cut(s) 56, 96, 152, 157, 195, 363, 676, 890
MlyI GAGTC 3 cut(s) 449, 587, 955
MmeI TCCRAC 1 cut(s) 347
MslI CAYNNNNRTG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 238
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 1108
NdeII GATC 1 cut(s) 870
NlaIV GGNNCC 2 cut(s) 618, 619
NmuCI GTSAC 1 cut(s) 958
PagI TCATGA 1 cut(s) 996
PfeI GAWTC 5 cut(s) 335, 398, 536, 719, 881
PflFI GACNNNGTC 2 cut(s) 355, 461
PkrI GCNGC 1 cut(s) 937
PleI GAGTC 3 cut(s) 448, 586, 955
PpsI GAGTC 3 cut(s) 448, 586, 955
PpuMI RGGWCCY 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 990
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 618, 619
PspPI GGNCC 2 cut(s) 617, 757
PspPPI RGGWCCY 1 cut(s) 617
PstNI CAGNNNCTG 1 cut(s) 237
PsyI GACNNNGTC 2 cut(s) 355, 461
RsaI GTAC 4 cut(s) 8, 74, 103, 911
RsaNI GTAC 4 cut(s) 7, 73, 102, 910
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 990
SatI GCNGC 1 cut(s) 936
Sau3AI GATC 1 cut(s) 870
Sau96I GGNCC 2 cut(s) 617, 757
ScaI AGTACT 4 cut(s) 8, 74, 103, 911
SchI GAGTC 3 cut(s) 449, 587, 955
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 1 cut(s) 990
SfaNI GCATC 1 cut(s) 1071
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
Sse9I AATT 8 cut(s) 56, 96, 152, 157, 195, 363, 676, 890
SsiI CCGC 1 cut(s) 202
SspMI CTAG 3 cut(s) 164, 798, 1106
SstI GAGCTC 1 cut(s) 990
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 939
TaqI TCGA 1 cut(s) 726
TasI AATT 8 cut(s) 56, 96, 152, 157, 195, 363, 676, 890
TatI WGTACW 4 cut(s) 6, 72, 101, 909
TfiI GAWTC 5 cut(s) 335, 398, 536, 719, 881
TscAI CASTG 3 cut(s) 132, 271, 780
TseFI GTSAC 1 cut(s) 958
TseI GCWGC 1 cut(s) 935
Tsp45I GTSAC 1 cut(s) 958
TspRI CASTG 3 cut(s) 132, 271, 780
Tth111I GACNNNGTC 2 cut(s) 355, 461
VpaK11BI GGWCC 1 cut(s) 617
XapI RAATTY 4 cut(s) 56, 152, 195, 890
XbaI TCTAGA 1 cut(s) 1105
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XspI CTAG 3 cut(s) 164, 798, 1106
ZrmI AGTACT 4 cut(s) 8, 74, 103, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.