Prupe.2G308900_v2.0.a1

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012960
Physical Location & Seq
Forward (+)
29289289 .. 29291182
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G308900.1

Sequence Viewer

Length: 828 bp
ATGCTGCTTTCTTCATTATATTTTCATTCTCTAATTCCCAAAGTTTCCAACTTTCACTCTGACTTTGACGTTTCTCTCATACCCATTTTCTCCCAAATGGATTCTGTCCAACTTCAGGTTCCAAAGATTGAGATTAACCCACACGGCTTCGACTCCCTACAGTACTATCAAGACCCATTTCATGAGAATGTTTCATTAGAAGAACATCTACCGCTCGACAATTTATTTGAGTTCAATTCAATCCCTTTGGAATGGAACCAGAACCAACATCCCCACATGGGTTTCCAAGGTTTTGAAGATTTAGGCAGCTGGGATTTCATGTTGGACAATACTGATTTCACATCACCTTGTGATTATGTTGTGGTTGATGAAAAGCCTGTGATCACCATGGTGGATTATTTTGATTACTCTAACAACCCTACAAGTGATTTGGATGAAGCTTCAAGAATGGCTGACTGGGGAAGTAATGGGATTTATAATAAGAAGTCGTCTGCTGCATTGGAATTGGATGAGATACAAAAATACTTTGACCTCCCCATCACTCAAGCAGCCAAGAAATTGAACGTGGGATTGACTGTTCTGAAGAGGAGGTGCAGGGAACTAAACATCATGAGGTGGCCACACAGAAAGATTAAGAGCTTGAAGGCTCTGATTGAAAATGTTAAGGGTTTGGGATTGACCGATGAGCTAAAGATGCTGGAGGAGCAGAAAAGGCTTCTAGAGCAACTGCCAGATATGGAGCTAACGGAGAGAGCCAAAAAGCTGAGGCAGGCTTCTTTCAAAGCCAATTACAAGATGAAGAGGTCTTTGACCACTGCTCGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

276

Amino Acids

31.98

Weight (kDa)

5.42

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AccBSI CCGCTC 1 cut(s) 214
AciI CCGC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 617
AcuI CTGAAG 2 cut(s) 98, 602
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 115, 278
AgsI TTSAA 8 cut(s) 235, 240, 296, 444, 562, 643, 656, 781
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 389
AloI GAACNNNNNNTCC 2 cut(s) 561, 593
AluBI AGCT 6 cut(s) 309, 440, 639, 688, 742, 763
AluI AGCT 6 cut(s) 309, 440, 639, 688, 742, 763
AoxI GGCC 1 cut(s) 617
ApeKI GCWGC 4 cut(s) 4, 306, 494, 548
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AsuHPI GGTGA 2 cut(s) 336, 376
BalI TGGCCA 1 cut(s) 619
BarI GAAGNNNNNNTAC 2 cut(s) 192, 224
BbvCI CCTCAGC 1 cut(s) 764
BbvI GCAGC 3 cut(s) 318, 481, 560
BccI CCATC 1 cut(s) 545
BceAI ACGGC 1 cut(s) 160
BclI TGATCA 1 cut(s) 381
BfaI CTAG 1 cut(s) 719
BfmI CTRYAG 1 cut(s) 158
BisI GCNGC 4 cut(s) 5, 307, 495, 549
BlsI GCNGC 4 cut(s) 6, 308, 496, 550
BmcAI AGTACT 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 120, 257
BmrI ACTGGG 1 cut(s) 466
BmsI GCATC 1 cut(s) 684
BmuI ACTGGG 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 719
Bpu10I CCTNAGC 1 cut(s) 764
BpuEI CTTGAG 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 286, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 278
Bse1I ACTGG 1 cut(s) 461
BseDI CCNNGG 2 cut(s) 286, 387
BseGI GGATG 3 cut(s) 268, 439, 514
BseLI CCNNNNNNNGG 2 cut(s) 115, 278
BseMII CTCAG 1 cut(s) 755
BseNI ACTGG 1 cut(s) 461
BseRI GAGGAG 2 cut(s) 601, 716
BseXI GCAGC 3 cut(s) 318, 481, 560
BseYI CCCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 613
BshFI GGCC 1 cut(s) 619
BslI CCNNNNNNNGG 2 cut(s) 115, 278
BsnI GGCC 1 cut(s) 619
Bsp143I GATC 1 cut(s) 381
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 1 cut(s) 212
BspANI GGCC 1 cut(s) 619
BspCNI CTCAG 1 cut(s) 756
BspHI TCATGA 2 cut(s) 181, 609
BspLI GGNNCC 2 cut(s) 120, 257
BsrBI CCGCTC 1 cut(s) 214
BsrI ACTGG 1 cut(s) 461
BssECI CCNNGG 2 cut(s) 286, 387
BssMI GATC 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 286, 387
Bst4CI ACNGT 2 cut(s) 162, 577
Bst6I CTCTTC 2 cut(s) 578, 794
BstC8I GCNNGC 1 cut(s) 771
BstDEI CTNAG 1 cut(s) 764
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 268, 439, 514
BstKTI GATC 1 cut(s) 384
BstMBI GATC 1 cut(s) 381
BstMWI GCNNNNNNNGC 4 cut(s) 694, 703, 712, 721
BstSFI CTRYAG 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 318, 481, 560
BsuRI GGCC 1 cut(s) 619
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 3 cut(s) 268, 439, 514
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 1 cut(s) 813
Cac8I GCNNGC 1 cut(s) 771
CciI TCATGA 2 cut(s) 181, 609
Csp6I GTAC 1 cut(s) 163
CviAII CATG 6 cut(s) 182, 277, 319, 388, 610, 825
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 1 cut(s) 764
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DraIII CACNNNGTG 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 617
Eam1104I CTCTTC 2 cut(s) 578, 794
EarI CTCTTC 2 cut(s) 578, 794
Eco130I CCWWGG 2 cut(s) 286, 387
Eco57I CTGAAG 2 cut(s) 98, 602
EcoT14I CCWWGG 2 cut(s) 286, 387
ErhI CCWWGG 2 cut(s) 286, 387
FaeI CATG 6 cut(s) 185, 280, 322, 391, 613, 828
FatI CATG 6 cut(s) 181, 276, 318, 387, 609, 824
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 4 cut(s) 5, 307, 495, 549
FokI GGATG 3 cut(s) 255, 446, 521
Fsp4HI GCNGC 4 cut(s) 5, 307, 495, 549
FspBI CTAG 1 cut(s) 719
GluI GCNGC 4 cut(s) 5, 307, 495, 549
GsaI CCCAGC 1 cut(s) 313
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 1 cut(s) 619
Hin1II CATG 6 cut(s) 185, 280, 322, 391, 613, 828
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 2 cut(s) 101, 152
HphI GGTGA 2 cut(s) 336, 376
Hpy188I TCNGA 3 cut(s) 61, 582, 651
Hpy188III TCNNGA 5 cut(s) 170, 182, 444, 610, 719
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 2 cut(s) 162, 577
HpyCH4IV ACGT 2 cut(s) 69, 564
HpyCH4V TGCA 2 cut(s) 497, 594
HpyF10VI GCNNNNNNNGC 4 cut(s) 694, 703, 712, 721
HpyF3I CTNAG 1 cut(s) 764
HpySE526I ACGT 2 cut(s) 69, 564
Hsp92II CATG 6 cut(s) 185, 280, 322, 391, 613, 828
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 1 cut(s) 381
LmnI GCTCC 2 cut(s) 703, 739
LpnPI CCDG 9 cut(s) 101, 272, 295, 390, 442, 580, 683, 744, 755
Lsp1109I GCAGC 3 cut(s) 318, 481, 560
LweI GCATC 1 cut(s) 684
MaeI CTAG 1 cut(s) 719
MaeII ACGT 2 cut(s) 69, 564
MalI GATC 1 cut(s) 383
MbiI CCGCTC 1 cut(s) 214
MboI GATC 1 cut(s) 381
MboII GAAGA 5 cut(s) 3, 212, 308, 595, 811
MlsI TGGCCA 1 cut(s) 619
MluCI AATT 6 cut(s) 33, 220, 235, 503, 557, 787
MluNI TGGCCA 1 cut(s) 619
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 3 cut(s) 72, 133, 303
MnlI CCTC 7 cut(s) 542, 579, 582, 606, 694, 759, 795
Mox20I TGGCCA 1 cut(s) 619
MscI TGGCCA 1 cut(s) 619
MseI TTAA 3 cut(s) 135, 633, 663
MslI CAYNNNNRTG 2 cut(s) 186, 389
Msp20I TGGCCA 1 cut(s) 619
MspA1I CMGCKG 1 cut(s) 309
MwoI GCNNNNNNNGC 4 cut(s) 694, 703, 712, 721
NcoI CCATGG 1 cut(s) 387
NdeII GATC 1 cut(s) 381
NlaIII CATG 6 cut(s) 185, 280, 322, 391, 613, 828
NlaIV GGNNCC 2 cut(s) 120, 257
OliI CACNNNNGTG 1 cut(s) 389
PagI TCATGA 2 cut(s) 181, 609
PfeI GAWTC 1 cut(s) 101
PkrI GCNGC 4 cut(s) 6, 308, 496, 550
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PsiI TTATAA 1 cut(s) 477
PspFI CCCAGC 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 120, 257
PvuII CAGCTG 1 cut(s) 309
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 2 cut(s) 186, 389
SaqAI TTAA 3 cut(s) 135, 633, 663
SatI GCNGC 4 cut(s) 5, 307, 495, 549
Sau3AI GATC 1 cut(s) 381
ScaI AGTACT 1 cut(s) 164
SchI GAGTC 1 cut(s) 146
SfaNI GCATC 1 cut(s) 684
SfcI CTRYAG 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 186, 389
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
Sse9I AATT 6 cut(s) 33, 220, 235, 503, 557, 787
SsiI CCGC 1 cut(s) 212
SspMI CTAG 1 cut(s) 719
StyI CCWWGG 2 cut(s) 286, 387
TaaI ACNGT 2 cut(s) 162, 577
TaiI ACGT 2 cut(s) 72, 567
TaqI TCGA 2 cut(s) 150, 216
TaqII GACCGA 1 cut(s) 695
TasI AATT 6 cut(s) 33, 220, 235, 503, 557, 787
TatI WGTACW 1 cut(s) 162
TfiI GAWTC 1 cut(s) 101
Tru1I TTAA 3 cut(s) 135, 633, 663
Tru9I TTAA 3 cut(s) 135, 633, 663
TscAI CASTG 1 cut(s) 820
TseI GCWGC 4 cut(s) 4, 306, 494, 548
TspDTI ATGAA 7 cut(s) 14, 170, 183, 307, 384, 450, 812
TspGWI ACGGA 1 cut(s) 761
TspRI CASTG 1 cut(s) 820
XbaI TCTAGA 1 cut(s) 718
XspI CTAG 1 cut(s) 719
ZrmI AGTACT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.