Rh1CG415000

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0012960
Physical Location & Seq
Forward (+)
67660071 .. 67662186
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG415000.1

Sequence Viewer

Length: 1254 bp
ATGGAGTACTCTACCCAACAACTTGTTATTCCAAAGATTGAAGAAAACCCACTTGAATTTGACTACCTAGAGTACTACTATCAAGACCCATTTCAAATTAGTACTACTTCGTTATTTGAAGCTCCACTGCTTGAAGATTTCCATGACCATGAATTCAATTCACTAGCTTTGCCATTATCAGAAGATTACAACCAAATTCCCCGCATGGGTTTTCAAGGACTTGAAGATTTCAGCACCTGGGACTTTACTGCTTTGGACATCACCACTGCTGTTTCATCACCATCTCATTGTGATAATCATGTGCTTGATGAAAAGCCTCTTCCAACAATGGTGGATTCTTTTGACTACTTGGACAATGTCCCAAGTGATTCTGGTCATGGTGAAGTTTCATTCAAGATTGAAACATCTGGTTTTTCATCATCTCATTGTGATTCTTTGGTGTTTGATGAAAAGCCTCTTACAACAATGGTGGAGTCCTTTACCTACTTGGACAATGTCCCAAGTGATTTGGGTCAGCATGAAGTTTCAATGATGACTGAAAGCTGTGGCTTTTCATCATCTCATTGTGATTCTGTGGTGTTCAATGAAAAGCCCATTTCAACAATGGTACAGTCATTTGACAACTTAAACAATGTCCCGAGTGTTCTGGGTCCCCATGAAGCTTCCATGAGGACTGAAAGCGCTACTGCTTTTGCGTCGTCCCATAGTAATTCTCTGGTGCTTTATGAAAAACCCCTTTCAACAATCGTGGATTCTTTCGACTACTCTGATATTCCTACACCTGATTTGGGCCATGATGAAGTTTCAGTGATCATTGAAAATGGGTTTCCTAGTTGGGACATATCAGACATTAGTTGTAAAAGAGAGACGAGTAATAGAAGGAGATATAATACAGAGATGAAGATCAAGTCTGATTCTTTGGAATTTGAGGAGATAAAGAAGTACTTTGACCTCCCCATAACAAAAGCAGCCAAGGAACTGAAGGTGGGAGTGACTCTGTTGAAGAGGAGGTGCAGGGAGCTCAACATCTTGAGGTGGCCTCACAGAAAGATTAGGAGCTTGAACTCTCTCATTGAAACTCTCAAGGGAATGGGTTTGAGAAATGAGATAAAGATGCTGGAGGAGAACAAAAGGCTTCTAGAGCAAGTGCCAAACATGGAGCTAACAAAAAGAACCAAAAAGCTGAGGCAGTCTATTTTCAAAGCTAACTACAACTACAAAAAGCAGATGTCTTTGTCTTTGTTGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

417

Amino Acids

47.7

Weight (kDa)

4.91

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 306 - 354 3.9e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 202
AcsI RAATTY 4 cut(s) 56, 152, 195, 923
AcuI CTGAAG 1 cut(s) 1001
AfaI GTAC 5 cut(s) 8, 74, 103, 609, 944
AfeI AGCGCT 1 cut(s) 682
AfiI CCNNNNNNNGG 2 cut(s) 206, 788
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 9 cut(s) 122, 167, 543, 662, 1021, 1059, 1162, 1183, 1205
AluI AGCT 9 cut(s) 122, 167, 543, 662, 1021, 1059, 1162, 1183, 1205
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 1 cut(s) 860
AlwNI CAGNNNCTG 1 cut(s) 237
Ama87I CYCGRG 1 cut(s) 637
Aor51HI AGCGCT 1 cut(s) 682
AoxI GGCC 2 cut(s) 790, 1037
ApeKI GCWGC 1 cut(s) 968
ApoI RAATTY 4 cut(s) 56, 152, 195, 923
AspLEI GCGC 1 cut(s) 683
AspS9I GGNCC 2 cut(s) 650, 790
AsuHPI GGTGA 3 cut(s) 253, 270, 392
AvaI CYCGRG 1 cut(s) 637
AvaII GGWCC 1 cut(s) 650
BanII GRGCYC 1 cut(s) 1023
Bbv12I GWGCWC 1 cut(s) 1023
BbvCI CCTCAGC 1 cut(s) 1184
BbvI GCAGC 1 cut(s) 980
BccI CCATC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 238
BclI TGATCA 1 cut(s) 810
BcoDI GTCTC 1 cut(s) 860
BfaI CTAG 4 cut(s) 68, 164, 831, 1139
BfoI RGCGCY 1 cut(s) 684
BisI GCNGC 1 cut(s) 969
BlsI GCNGC 1 cut(s) 970
BmcAI AGTACT 4 cut(s) 8, 74, 103, 944
Bme1390I CCNGG 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 650
BmeT110I CYCGRG 1 cut(s) 637
BmgT120I GGNCC 2 cut(s) 650, 790
BmiI GGNNCC 2 cut(s) 651, 652
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 1104
BplI GAGNNNNNCTC 2 cut(s) 1024, 1056
BpmI CTGGAG 1 cut(s) 1139
Bpu10I CCTNAGC 1 cut(s) 1184
BpuEI CTTGAG 2 cut(s) 1051, 1067
BsaBI GATNNNNATC 1 cut(s) 902
BsaJI CCNNGG 2 cut(s) 237, 972
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 788
Bse8I GATNNNNATC 1 cut(s) 902
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 2 cut(s) 237, 972
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 2 cut(s) 206, 788
BseMII CTCAG 1 cut(s) 1175
BseRI GAGGAG 3 cut(s) 944, 1021, 1136
BseXI GCAGC 1 cut(s) 980
BsgI GTGCAG 1 cut(s) 1033
BshFI GGCC 2 cut(s) 792, 1039
BsiHKAI GWGCWC 1 cut(s) 1023
BsiHKCI CYCGRG 1 cut(s) 637
BslFI GGGAC 7 cut(s) 254, 344, 482, 620, 636, 685, 851
BslI CCNNNNNNNGG 2 cut(s) 206, 788
BsmAI GTCTC 1 cut(s) 860
BsmBI CGTCTC 1 cut(s) 860
BsmFI GGGAC 7 cut(s) 254, 344, 482, 620, 636, 685, 851
BsnI GGCC 2 cut(s) 792, 1039
BsoBI CYCGRG 1 cut(s) 637
Bsp1286I GDGCHC 1 cut(s) 1023
Bsp143I GATC 2 cut(s) 810, 903
BspACI CCGC 1 cut(s) 202
BspANI GGCC 2 cut(s) 792, 1039
BspCNI CTCAG 1 cut(s) 1176
BspLI GGNNCC 2 cut(s) 651, 652
BssECI CCNNGG 2 cut(s) 237, 972
BssMI GATC 2 cut(s) 810, 903
BssT1I CCWWGG 1 cut(s) 972
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 612
Bst6I CTCTTC 2 cut(s) 324, 998
BstDEI CTNAG 1 cut(s) 1184
BstH2I RGCGCY 1 cut(s) 684
BstHHI GCGC 1 cut(s) 683
BstKTI GATC 2 cut(s) 813, 906
BstMAI GTCTC 1 cut(s) 860
BstMBI GATC 2 cut(s) 810, 903
BstMWI GCNNNNNNNGC 1 cut(s) 1141
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 980
BsuRI GGCC 2 cut(s) 792, 1039
BtsI GCAGTG 2 cut(s) 125, 264
BtsIMutI CAGTG 3 cut(s) 125, 264, 813
CaiI CAGNNNCTG 1 cut(s) 237
CfoI GCGC 1 cut(s) 683
Cfr13I GGNCC 2 cut(s) 650, 790
CseI GACGC 1 cut(s) 684
Csp6I GTAC 5 cut(s) 7, 73, 102, 608, 943
CspCI CAANNNNNGTGG 6 cut(s) 312, 347, 450, 485, 729, 764
CviQI GTAC 5 cut(s) 7, 73, 102, 608, 943
DdeI CTNAG 1 cut(s) 1184
DpnI GATC 2 cut(s) 812, 905
DpnII GATC 2 cut(s) 810, 903
Eam1104I CTCTTC 2 cut(s) 324, 998
EarI CTCTTC 2 cut(s) 324, 998
Ecl136II GAGCTC 1 cut(s) 1021
Eco130I CCWWGG 1 cut(s) 972
Eco24I GRGCYC 1 cut(s) 1023
Eco47I GGWCC 1 cut(s) 650
Eco47III AGCGCT 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 1021
Eco57I CTGAAG 1 cut(s) 1001
Eco88I CYCGRG 1 cut(s) 637
EcoICRI GAGCTC 1 cut(s) 1021
EcoO109I RGGNCCY 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 1 cut(s) 972
EcoT38I GRGCYC 1 cut(s) 1023
ErhI CCWWGG 1 cut(s) 972
Esp3I CGTCTC 1 cut(s) 860
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 7 cut(s) 254, 344, 482, 620, 636, 685, 851
FauI CCCGC 1 cut(s) 209
FbaI TGATCA 1 cut(s) 810
Fnu4HI GCNGC 1 cut(s) 969
FriOI GRGCYC 1 cut(s) 1023
Fsp4HI GCNGC 1 cut(s) 969
FspBI CTAG 4 cut(s) 68, 164, 831, 1139
GlaI GCGC 1 cut(s) 682
GluI GCNGC 1 cut(s) 969
GsuI CTGGAG 1 cut(s) 1139
HaeII RGCGCY 1 cut(s) 684
HaeIII GGCC 2 cut(s) 792, 1039
HgaI GACGC 1 cut(s) 684
HhaI GCGC 1 cut(s) 683
Hin6I GCGC 1 cut(s) 681
HinP1I GCGC 1 cut(s) 681
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 8 cut(s) 335, 368, 431, 473, 569, 752, 914, 994
HphI GGTGA 3 cut(s) 253, 270, 392
Hpy188I TCNGA 4 cut(s) 181, 769, 847, 913
Hpy188III TCNNGA 5 cut(s) 83, 394, 637, 1030, 1139
Hpy99I CGWCG 1 cut(s) 700
HpyAV CCTTC 2 cut(s) 873, 976
HpyCH4III ACNGT 1 cut(s) 612
HpyCH4V TGCA 1 cut(s) 1014
HpyF10VI GCNNNNNNNGC 1 cut(s) 1141
HpyF3I CTNAG 1 cut(s) 1184
HspAI GCGC 1 cut(s) 681
KflI GGGWCCC 1 cut(s) 650
Ksp22I TGATCA 1 cut(s) 810
Kzo9I GATC 2 cut(s) 810, 903
LmnI GCTCC 4 cut(s) 127, 1018, 1056, 1159
LpnPI CCDG 9 cut(s) 223, 250, 357, 393, 632, 701, 795, 1000, 1103
Lsp1109I GCAGC 1 cut(s) 980
LweI GCATC 1 cut(s) 1104
MaeI CTAG 4 cut(s) 68, 164, 831, 1139
MaeIII GTNAC 1 cut(s) 991
MalI GATC 2 cut(s) 812, 905
MboI GATC 2 cut(s) 810, 903
MboII GAAGA 7 cut(s) 53, 146, 194, 236, 311, 913, 1015
MhlI GDGCHC 1 cut(s) 1023
MluCI AATT 7 cut(s) 56, 96, 152, 157, 195, 709, 923
MlyI GAGTC 2 cut(s) 482, 988
MmeI TCCRAC 1 cut(s) 347
MseI TTAA 1 cut(s) 626
MslI CAYNNNNRTG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 238
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 1 cut(s) 1141
NdeII GATC 2 cut(s) 810, 903
NlaIV GGNNCC 2 cut(s) 651, 652
NmuCI GTSAC 1 cut(s) 991
PfeI GAWTC 6 cut(s) 335, 368, 431, 569, 752, 914
PflFI GACNNNGTC 2 cut(s) 356, 494
PkrI GCNGC 1 cut(s) 970
PleI GAGTC 2 cut(s) 481, 988
PpsI GAGTC 2 cut(s) 481, 988
PpuMI RGGWCCY 1 cut(s) 650
Psp124BI GAGCTC 1 cut(s) 1023
Psp5II RGGWCCY 1 cut(s) 650
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 2 cut(s) 651, 652
PspPI GGNCC 2 cut(s) 650, 790
PspPPI RGGWCCY 1 cut(s) 650
PstNI CAGNNNCTG 1 cut(s) 237
PsyI GACNNNGTC 2 cut(s) 356, 494
RsaI GTAC 5 cut(s) 8, 74, 103, 609, 944
RsaNI GTAC 5 cut(s) 7, 73, 102, 608, 943
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 1023
SaqAI TTAA 1 cut(s) 626
SatI GCNGC 1 cut(s) 969
Sau3AI GATC 2 cut(s) 810, 903
Sau96I GGNCC 2 cut(s) 650, 790
ScaI AGTACT 4 cut(s) 8, 74, 103, 944
SchI GAGTC 2 cut(s) 482, 988
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 1 cut(s) 1023
SfaNI GCATC 1 cut(s) 1104
SinI GGWCC 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 1030, 1082
SmoI CTYRAG 2 cut(s) 1030, 1082
Sse9I AATT 7 cut(s) 56, 96, 152, 157, 195, 709, 923
SsiI CCGC 1 cut(s) 202
SspMI CTAG 4 cut(s) 68, 164, 831, 1139
SstI GAGCTC 1 cut(s) 1023
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 1 cut(s) 972
TaaI ACNGT 1 cut(s) 612
TaqI TCGA 1 cut(s) 759
TasI AATT 7 cut(s) 56, 96, 152, 157, 195, 709, 923
TatI WGTACW 4 cut(s) 6, 72, 101, 942
TfiI GAWTC 6 cut(s) 335, 368, 431, 569, 752, 914
Tru1I TTAA 1 cut(s) 626
Tru9I TTAA 1 cut(s) 626
TscAI CASTG 3 cut(s) 132, 271, 813
TseFI GTSAC 1 cut(s) 991
TseI GCWGC 1 cut(s) 968
Tsp45I GTSAC 1 cut(s) 991
TspRI CASTG 3 cut(s) 132, 271, 813
Tth111I GACNNNGTC 2 cut(s) 356, 494
VpaK11BI GGWCC 1 cut(s) 650
XapI RAATTY 4 cut(s) 56, 152, 195, 923
XbaI TCTAGA 1 cut(s) 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XspI CTAG 4 cut(s) 68, 164, 831, 1139
ZrmI AGTACT 4 cut(s) 8, 74, 103, 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.