FvH4_7g31350

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012960
Physical Location & Seq
Forward (+)
22679956 .. 22681574
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31350.t1

Sequence Viewer

Length: 1446 bp
ATGGAGTACAATACCCAACAACTTGTTGATCCAGAGATTGAAGAAAACCCACTTGAATGTGACTACTTAGGGTACCACTATCAAGACCCATTGCCAAACAGTACTACTTCCATGTTTGAGGCTCCACAGATTGATCATGGATTCAATTCAGTAGCTTTGCCATTACCTGAAGATAACAACCAAATTCCCCACATGGGTTTTCAAGGACTTGAAGATTTTAGCCTCCGGGACTACACAGCTTTGGACATTGCTAATACTTTTTCATCATCACCTCTCTGTGATTTTAATAACGTACTTGATGGAAAGACTCATACAACAGTATTGGAGTCTTTTGGTGAGTCTAGCAATGACCCAAGTGACATGATGATCGGAAGTTCTGGTTTTTCATCGACTCAATGTGATTCAGTGTTGTTTGATGAAAAGCCCATTTCAACAATGGTGGATTCTTTTGATTATTTGATCAACAACTCAACTAATTTGGGTTACCATGCTATGATTGAAAGCTCTGGTTTTTCATCATCTCTCTGTGATTCTCTGGTCTTCGATGATATGCTTCTTAGAGAAATGGTGGAGTCTTTGAACAACAATGTCCCAAGTGATGTTGGTCAGCATGAAGTTTCAATGATGGTTGAAAGTTCTCGGTTTTCATCGACTCAATGTGATTCTGTTGTGTTTGATGAAAAGCCCATTTCAACAATGGTGGATTCTTTTGATTATTTGATCAACAACTCAACTAATTTGGGTCACCATGCTATGATTGAAAGCTCTGGTTTTTCATCATCTCTCTGTGATTCTTTGGTCTTTGATGAAATGCTTCTTAGAGAAATAGTGGAATCCTTGAACAACAATGTCCCAAGTGATGTTGGTCAGCATGAAGTTTCAATGATGGTTGGAAGTTCTGGTTTTTCATCGACTCAATGTGATTCTGTTGTGTTCGATGAAAAGCCCATTTCAACAATCGTAGAGTCCTTTGGCAACTCGAGCAATGTCCCGAGTGTTTTTGATCACCATGAAGCTTCCATGGTGACTGAAAGTTCTGCTGCTTATTCATCCTCTCATTGTAATGCTCTGGTGCTATATGAAACACCCCTTTCAACAATCGTGGATTCTTTCGACTACTCTGATATGCCTACACCTGATTTGAGCGATGAAGAAGTTTCAGTGAAGATTGAAAGTGGGTATCCTAGTTGGGGCACTTCAGACACTAGTTGTTTGAGAGGGACAAGTAATAGAGGGAGATGTAGTACACAGATGAAGATCAAGTCTGATGCTTTGGAATTGGAGGGTATAAAGCAGTACTTTAACCTCCCAATAACAAAAGCAGCCAAGGAATTGAAGGTGGGAGTGACATTGCTGAAGAGGAGATGCAGGGAGCTCAACATCATGAGGTGGCCTCATAGAAAGATCAAGAGCTTGAATTCTCTTATTGAAACTATGAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

482

Amino Acids

53.25

Weight (kDa)

4.34

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 425 - 472 4.4e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 72
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 2 cut(s) 183, 1417
AcuI CTGAAG 3 cut(s) 189, 1182, 1376
AfaI GTAC 6 cut(s) 8, 74, 103, 294, 1246, 1298
AfiI CCNNNNNNNGG 2 cut(s) 194, 1190
AhlI ACTAGT 1 cut(s) 1205
AluBI AGCT 7 cut(s) 155, 239, 504, 765, 1016, 1375, 1413
AluI AGCT 7 cut(s) 155, 239, 504, 765, 1016, 1375, 1413
Alw21I GWGCWC 1 cut(s) 1377
AlwI GGATC 1 cut(s) 23
Ama87I CYCGRG 2 cut(s) 979, 991
AoxI GGCC 1 cut(s) 1391
ApeKI GCWGC 2 cut(s) 1040, 1322
ApoI RAATTY 2 cut(s) 183, 1417
Asp700I GAANNNNTTC 1 cut(s) 813
Asp718I GGTACC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 5 cut(s) 261, 347, 737, 998, 1036
AvaI CYCGRG 2 cut(s) 979, 991
BaeGI GKGCMC 1 cut(s) 1196
BanI GGYRCC 1 cut(s) 72
BanII GRGCYC 1 cut(s) 1377
BbsI GAAGAC 1 cut(s) 532
Bbv12I GWGCWC 1 cut(s) 1377
BbvI GCAGC 2 cut(s) 1027, 1334
BccI CCATC 3 cut(s) 293, 619, 880
BciVI GTATCC 1 cut(s) 1191
BclI TGATCA 4 cut(s) 133, 459, 720, 1003
BcnI CCSGG 1 cut(s) 227
BcuI ACTAGT 1 cut(s) 1205
BfaI CTAG 3 cut(s) 342, 1185, 1206
BfuI GTATCC 1 cut(s) 1191
BisI GCNGC 2 cut(s) 1041, 1323
BlsI GCNGC 2 cut(s) 1042, 1324
BmcAI AGTACT 2 cut(s) 103, 1298
Bme1390I CCNGG 1 cut(s) 227
BmeT110I CYCGRG 2 cut(s) 979, 991
BmiI GGNNCC 2 cut(s) 74, 123
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 1258, 1355
BpiI GAAGAC 1 cut(s) 532
BplI GAGNNNNNCTC 2 cut(s) 1378, 1410
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 1256
BsaJI CCNNGG 2 cut(s) 1020, 1326
BsaXI ACNNNNNCTCC 2 cut(s) 1228, 1258
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 1190
Bse3DI GCAATG 5 cut(s) 89, 246, 352, 991, 1349
Bse8I GATNNNNATC 1 cut(s) 1256
BseDI CCNNGG 2 cut(s) 1020, 1326
BseGI GGATG 1 cut(s) 1049
BseJI GATNNNNATC 1 cut(s) 1256
BseLI CCNNNNNNNGG 2 cut(s) 194, 1190
BseMI GCAATG 5 cut(s) 89, 246, 352, 991, 1349
BseRI GAGGAG 1 cut(s) 1375
BseSI GKGCMC 1 cut(s) 1196
BseXI GCAGC 2 cut(s) 1027, 1334
BshFI GGCC 1 cut(s) 1393
BshNI GGYRCC 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 1377
BsiHKCI CYCGRG 2 cut(s) 979, 991
BsiSI CCGG 1 cut(s) 226
BslFI GGGAC 5 cut(s) 242, 575, 836, 974, 1234
BslI CCNNNNNNNGG 2 cut(s) 194, 1190
BsmFI GGGAC 5 cut(s) 242, 575, 836, 974, 1234
BsnI GGCC 1 cut(s) 1393
BsoBI CYCGRG 2 cut(s) 979, 991
Bsp1286I GDGCHC 2 cut(s) 1196, 1377
Bsp143I GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
Bsp19I CCATGG 1 cut(s) 1020
BspANI GGCC 1 cut(s) 1393
BspHI TCATGA 1 cut(s) 1383
BspLI GGNNCC 2 cut(s) 74, 123
BspPI GGATC 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 72
BsrDI GCAATG 5 cut(s) 89, 246, 352, 991, 1349
BssECI CCNNGG 2 cut(s) 1020, 1326
BssMI GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
BssT1I CCWWGG 2 cut(s) 1020, 1326
Bst4CI ACNGT 2 cut(s) 101, 319
Bst6I CTCTTC 1 cut(s) 1352
BstDEI CTNAG 3 cut(s) 67, 557, 818
BstDSI CCRYGG 1 cut(s) 1020
BstEII GGTNACC 2 cut(s) 482, 743
BstF5I GGATG 1 cut(s) 1049
BstKTI GATC 8 cut(s) 31, 136, 369, 462, 723, 1006, 1260, 1407
BstMBI GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
BstMWI GCNNNNNNNGC 1 cut(s) 981
BstPI GGTNACC 2 cut(s) 482, 743
BstSCI CCNGG 1 cut(s) 225
BstSLI GKGCMC 1 cut(s) 1196
BstV1I GCAGC 2 cut(s) 1027, 1334
BstV2I GAAGAC 1 cut(s) 532
BsuI GTATCC 1 cut(s) 1191
BsuRI GGCC 1 cut(s) 1393
BtgI CCRYGG 1 cut(s) 1020
BtgZI GCGATG 1 cut(s) 1161
BtsCI GGATG 1 cut(s) 1049
BtsIMutI CAGTG 2 cut(s) 411, 1167
CciI TCATGA 1 cut(s) 1383
Csp6I GTAC 6 cut(s) 7, 73, 102, 293, 1245, 1297
CspCI CAANNNNNGTGG 6 cut(s) 420, 455, 681, 716, 1083, 1118
CviQI GTAC 6 cut(s) 7, 73, 102, 293, 1245, 1297
DdeI CTNAG 3 cut(s) 67, 557, 818
DpnI GATC 8 cut(s) 30, 135, 368, 461, 722, 1005, 1259, 1406
DpnII GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
Eam1104I CTCTTC 1 cut(s) 1352
EarI CTCTTC 1 cut(s) 1352
Ecl136II GAGCTC 1 cut(s) 1375
Eco130I CCWWGG 2 cut(s) 1020, 1326
Eco24I GRGCYC 1 cut(s) 1377
Eco53kI GAGCTC 1 cut(s) 1375
Eco57I CTGAAG 3 cut(s) 189, 1182, 1376
Eco88I CYCGRG 2 cut(s) 979, 991
Eco91I GGTNACC 2 cut(s) 482, 743
EcoICRI GAGCTC 1 cut(s) 1375
EcoO65I GGTNACC 2 cut(s) 482, 743
EcoRI GAATTC 1 cut(s) 1417
EcoT14I CCWWGG 2 cut(s) 1020, 1326
EcoT38I GRGCYC 1 cut(s) 1377
ErhI CCWWGG 2 cut(s) 1020, 1326
FalI AAGNNNNNCTT 2 cut(s) 1283, 1315
FaqI GGGAC 5 cut(s) 242, 575, 836, 974, 1234
FbaI TGATCA 4 cut(s) 133, 459, 720, 1003
Fnu4HI GCNGC 2 cut(s) 1041, 1323
FokI GGATG 1 cut(s) 1036
FriOI GRGCYC 1 cut(s) 1377
Fsp4HI GCNGC 2 cut(s) 1041, 1323
FspBI CTAG 3 cut(s) 342, 1185, 1206
GluI GCNGC 2 cut(s) 1041, 1323
HaeIII GGCC 1 cut(s) 1393
HapII CCGG 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 1014
HpaII CCGG 1 cut(s) 226
HphI GGTGA 5 cut(s) 261, 347, 737, 998, 1036
Hpy166II GTNNAC 1 cut(s) 1247
Hpy188I TCNGA 4 cut(s) 371, 1123, 1201, 1267
Hpy188III TCNNGA 5 cut(s) 32, 83, 991, 1384, 1408
Hpy8I GTNNAC 1 cut(s) 1247
HpyAV CCTTC 2 cut(s) 1330, 1432
HpyCH4III ACNGT 2 cut(s) 101, 319
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 1 cut(s) 1368
HpyF10VI GCNNNNNNNGC 1 cut(s) 981
HpyF3I CTNAG 3 cut(s) 67, 557, 818
HpySE526I ACGT 1 cut(s) 291
KpnI GGTACC 1 cut(s) 76
Ksp22I TGATCA 4 cut(s) 133, 459, 720, 1003
Kzo9I GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
LmnI GCTCC 2 cut(s) 127, 1372
Lsp1109I GCAGC 2 cut(s) 1027, 1334
LweI GCATC 2 cut(s) 1258, 1355
MaeI CTAG 3 cut(s) 342, 1185, 1206
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 6 cut(s) 59, 356, 482, 743, 1024, 1345
MalI GATC 8 cut(s) 30, 135, 368, 461, 722, 1005, 1259, 1406
MboI GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
MboII GAAGA 8 cut(s) 53, 182, 224, 532, 1163, 1177, 1267, 1369
MhlI GDGCHC 2 cut(s) 1196, 1377
MluCI AATT 7 cut(s) 145, 183, 475, 736, 1277, 1331, 1417
MlyI GAGTC 8 cut(s) 301, 335, 347, 385, 581, 646, 907, 974
MmeI TCCRAC 1 cut(s) 871
MroXI GAANNNNTTC 1 cut(s) 813
MseI TTAA 2 cut(s) 285, 1302
MslI CAYNNNNRTG 2 cut(s) 55, 1062
MspI CCGG 1 cut(s) 226
MspR9I CCNGG 1 cut(s) 227
MwoI GCNNNNNNNGC 1 cut(s) 981
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 1 cut(s) 1020
NdeII GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
NlaIV GGNNCC 2 cut(s) 74, 123
NmuCI GTSAC 5 cut(s) 59, 356, 743, 1024, 1345
PaeR7I CTCGAG 1 cut(s) 979
PagI TCATGA 1 cut(s) 1383
PdmI GAANNNNTTC 1 cut(s) 813
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 2 cut(s) 1042, 1324
PleI GAGTC 8 cut(s) 301, 334, 346, 385, 580, 646, 907, 973
PpsI GAGTC 8 cut(s) 301, 334, 346, 385, 580, 646, 907, 973
Psp124BI GAGCTC 1 cut(s) 1377
PspEI GGTNACC 2 cut(s) 482, 743
PspN4I GGNNCC 2 cut(s) 74, 123
PspXI VCTCGAGB 1 cut(s) 979
RsaI GTAC 6 cut(s) 8, 74, 103, 294, 1246, 1298
RsaNI GTAC 6 cut(s) 7, 73, 102, 293, 1245, 1297
RseI CAYNNNNRTG 2 cut(s) 55, 1062
SacI GAGCTC 1 cut(s) 1377
SaqAI TTAA 2 cut(s) 285, 1302
SatI GCNGC 2 cut(s) 1041, 1323
Sau3AI GATC 8 cut(s) 28, 133, 366, 459, 720, 1003, 1257, 1404
ScaI AGTACT 2 cut(s) 103, 1298
SchI GAGTC 8 cut(s) 301, 335, 347, 385, 581, 646, 907, 974
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 2 cut(s) 1196, 1377
SfaNI GCATC 2 cut(s) 1258, 1355
Sfr274I CTCGAG 1 cut(s) 979
SlaI CTCGAG 1 cut(s) 979
SmiMI CAYNNNNRTG 2 cut(s) 55, 1062
SmlI CTYRAG 1 cut(s) 979
SmoI CTYRAG 1 cut(s) 979
SpeI ACTAGT 1 cut(s) 1205
Sse9I AATT 7 cut(s) 145, 183, 475, 736, 1277, 1331, 1417
SspMI CTAG 3 cut(s) 342, 1185, 1206
SstI GAGCTC 1 cut(s) 1377
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 2 cut(s) 1020, 1326
TaaI ACNGT 2 cut(s) 101, 319
TaiI ACGT 1 cut(s) 294
TaqI TCGA 7 cut(s) 389, 543, 650, 911, 936, 980, 1113
TasI AATT 7 cut(s) 145, 183, 475, 736, 1277, 1331, 1417
TatI WGTACW 4 cut(s) 6, 101, 1244, 1296
Tru1I TTAA 2 cut(s) 285, 1302
Tru9I TTAA 2 cut(s) 285, 1302
TscAI CASTG 2 cut(s) 411, 1167
TseFI GTSAC 5 cut(s) 59, 356, 743, 1024, 1345
TseI GCWGC 2 cut(s) 1040, 1322
Tsp45I GTSAC 5 cut(s) 59, 356, 743, 1024, 1345
TspRI CASTG 2 cut(s) 411, 1167
XapI RAATTY 2 cut(s) 183, 1417
XcmI CCANNNNNNNNNTGG 2 cut(s) 433, 694
XhoI CTCGAG 1 cut(s) 979
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 3 cut(s) 342, 1185, 1206
ZrmI AGTACT 2 cut(s) 103, 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.