RLG00000026302

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012960
Physical Location & Seq
Reverse (-)
1992656 .. 1994429
1774 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026302

Sequence Viewer

Length: 1248 bp
ATGGATTACTCTACCGAAAAACTTGTTATTCCAAAGAATGAAGAAAACCCACTTGAATTTGACTACTTAGAGTATCAAGACCCATTTCAAAACAGTACTTCTTCCATGTTTGAAGCTCCACAGCTTGAAAATTTCCCTGACCATGAATTCAATTCACTAGCTTTGCCATTACCAGAAGATTACAACCAAATTCCCCACGTGGATTTTCAAGGACTTGAAGATTTTAGCACCTGGGACTTTACTGCTTTGGACATCACCACTACTGTTTCATCACCAACTCATTGTGATAATCATGTGCTTGATGAAAAGCCTCTTCCAACAATGGTGGATTCTTTTGACAACTTGGACAATGTCCCAAGTGATTCTAGTCATGGTGAAGTTTCATTTAAGATTGAAAGCTCTGGTTTTTCATCATCTCATTGTGATTCTTTTGTGTTTGATGAAAAGCCTCTTACAACAATGGTGGAGTCCTTTACCTACTTGCACAATGTCCCAAGTGATTTGGGTCAGCATGAAGTTTCAATGATGACTGAAAGCTGTGGCTTTTCATCATCTCATTGTGATTCGGTGGTGTTCAACGAAAAGCCCATTTCAACAATGGTAGAGTCATTTGACAACTCAAACAATGTCCCGAGTGTTTTGGGTCACCATGAAGCTTTCATGAGGACTGAAAGCGCTACTGCTTTTGCGTCGTCCCATAGTAATTCTCTGGTGCTTTATGAAAAACCCCTTTCAACAATCGTGGATTCTTTCGACTACTCTGATATTCCTACACCTGATTTGAGCCATGATGAAGTTTCAGTGATGATTGAAAATGGGTTTCCTAGTTGGGACATATCAGACATTAGTTGTAAAAGAGAGACAAGTAATAGAAGGAGATATAATACAGAGATGAAGATGAAGTCTGATTCTTTGGAATTTGAGGAGATAAAGAACTACTTTGACCTCCCCATAGCAAAAGCAGCCAAGGAACTGAAGGTGGGAGTGACTCAGTTGAAGAGGAGGTGCAGGGAGCTCAACATCATGAGGTGGCCTCACAGAAAGATTAGGAGCTTGAACTCTCTCATTGAAACTCTCAAGGGAATGGGTTTGAGAAATGAGGTAAAGATGCTGGAGGAGAACAAAAGGCTTCTAGAGCAAGTGCCAAACATGGAGCTAACAAAGAGAACCAAAAAGTTGAGGCAGTCTATTTTCAAAGCCAACTACAACTACAAAAAGCAGATGTCTTTGTCTTTGTTGCTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

416

Amino Acids

47.54

Weight (kDa)

4.91

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 304 - 352 1.3e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 5 cut(s) 56, 130, 146, 189, 917
AcuI CTGAAG 1 cut(s) 995
AcvI CACGTG 1 cut(s) 199
AfaI GTAC 1 cut(s) 97
AfeI AGCGCT 1 cut(s) 676
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 9 cut(s) 116, 124, 161, 399, 537, 656, 1015, 1053, 1156
AluI AGCT 9 cut(s) 116, 124, 161, 399, 537, 656, 1015, 1053, 1156
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 1 cut(s) 854
Ama87I CYCGRG 1 cut(s) 631
Aor51HI AGCGCT 1 cut(s) 676
AoxI GGCC 1 cut(s) 1031
ApeKI GCWGC 1 cut(s) 962
ApoI RAATTY 5 cut(s) 56, 130, 146, 189, 917
AspLEI GCGC 1 cut(s) 677
AsuHPI GGTGA 4 cut(s) 247, 264, 386, 638
AvaI CYCGRG 1 cut(s) 631
BanII GRGCYC 1 cut(s) 1017
BbrPI CACGTG 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 1017
BbvI GCAGC 1 cut(s) 974
BciT130I CCWGG 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 854
BfaI CTAG 4 cut(s) 158, 366, 825, 1133
BfoI RGCGCY 1 cut(s) 678
BisI GCNGC 1 cut(s) 963
BlsI GCNGC 1 cut(s) 964
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 631
BmrFI CCNGG 1 cut(s) 232
BmsI GCATC 1 cut(s) 1098
BplI GAGNNNNNCTC 2 cut(s) 1018, 1050
BpmI CTGGAG 1 cut(s) 1133
BpuEI CTTGAG 1 cut(s) 1061
BsaAI YACGTR 1 cut(s) 199
BsaJI CCNNGG 2 cut(s) 231, 966
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 2 cut(s) 231, 966
BseMII CTCAG 1 cut(s) 1004
BseRI GAGGAG 3 cut(s) 938, 1015, 1130
BseXI GCAGC 1 cut(s) 974
BsgI GTGCAG 1 cut(s) 1027
BshFI GGCC 1 cut(s) 1033
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 1 cut(s) 631
BslFI GGGAC 6 cut(s) 248, 338, 476, 614, 679, 845
BsmAI GTCTC 1 cut(s) 854
BsmFI GGGAC 6 cut(s) 248, 338, 476, 614, 679, 845
BsnI GGCC 1 cut(s) 1033
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 1 cut(s) 1017
BspANI GGCC 1 cut(s) 1033
BspCNI CTCAG 1 cut(s) 1003
BspHI TCATGA 2 cut(s) 660, 1023
BssECI CCNNGG 2 cut(s) 231, 966
BssT1I CCWWGG 1 cut(s) 966
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 95, 265
Bst6I CTCTTC 2 cut(s) 318, 992
BstBAI YACGTR 1 cut(s) 199
BstDEI CTNAG 2 cut(s) 67, 990
BstEII GGTNACC 1 cut(s) 644
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstMAI GTCTC 1 cut(s) 854
BstMWI GCNNNNNNNGC 2 cut(s) 962, 1135
BstNI CCWGG 1 cut(s) 232
BstPI GGTNACC 1 cut(s) 644
BstSCI CCNGG 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 974
BsuRI GGCC 1 cut(s) 1033
BtsIMutI CAGTG 1 cut(s) 807
CciI TCATGA 2 cut(s) 660, 1023
CfoI GCGC 1 cut(s) 677
CseI GACGC 1 cut(s) 678
Csp6I GTAC 1 cut(s) 96
CspCI CAANNNNNGTGG 6 cut(s) 306, 341, 444, 479, 723, 758
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 2 cut(s) 67, 990
Eam1104I CTCTTC 2 cut(s) 318, 992
EarI CTCTTC 2 cut(s) 318, 992
Ecl136II GAGCTC 1 cut(s) 1015
Eco130I CCWWGG 1 cut(s) 966
Eco24I GRGCYC 1 cut(s) 1017
Eco47III AGCGCT 1 cut(s) 676
Eco53kI GAGCTC 1 cut(s) 1015
Eco57I CTGAAG 1 cut(s) 995
Eco72I CACGTG 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 631
Eco91I GGTNACC 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 1015
EcoO65I GGTNACC 1 cut(s) 644
EcoRI GAATTC 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 230
EcoT14I CCWWGG 1 cut(s) 966
EcoT38I GRGCYC 1 cut(s) 1017
ErhI CCWWGG 1 cut(s) 966
FalI AAGNNNNNCTT 2 cut(s) 923, 955
FaqI GGGAC 6 cut(s) 248, 338, 476, 614, 679, 845
Fnu4HI GCNGC 1 cut(s) 963
FriOI GRGCYC 1 cut(s) 1017
Fsp4HI GCNGC 1 cut(s) 963
FspBI CTAG 4 cut(s) 158, 366, 825, 1133
GlaI GCGC 1 cut(s) 676
GluI GCNGC 1 cut(s) 963
GsuI CTGGAG 1 cut(s) 1133
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 1 cut(s) 1033
HgaI GACGC 1 cut(s) 678
HhaI GCGC 1 cut(s) 677
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 9 cut(s) 329, 362, 425, 467, 563, 605, 746, 908, 988
HphI GGTGA 4 cut(s) 247, 264, 386, 638
Hpy188I TCNGA 3 cut(s) 763, 841, 907
Hpy188III TCNNGA 5 cut(s) 77, 631, 661, 1024, 1133
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 2 cut(s) 867, 970
HpyCH4III ACNGT 2 cut(s) 95, 265
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 2 cut(s) 484, 1008
HpyF10VI GCNNNNNNNGC 2 cut(s) 962, 1135
HpyF3I CTNAG 2 cut(s) 67, 990
HpySE526I ACGT 1 cut(s) 198
HspAI GCGC 1 cut(s) 675
LmnI GCTCC 4 cut(s) 121, 1012, 1050, 1153
LpnPI CCDG 9 cut(s) 150, 186, 217, 244, 387, 695, 789, 994, 1097
Lsp1109I GCAGC 1 cut(s) 974
LweI GCATC 1 cut(s) 1098
MaeI CTAG 4 cut(s) 158, 366, 825, 1133
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 2 cut(s) 644, 985
MboII GAAGA 7 cut(s) 53, 93, 188, 230, 305, 907, 1009
MhlI GDGCHC 1 cut(s) 1017
MluCI AATT 7 cut(s) 56, 130, 146, 151, 189, 703, 917
MlyI GAGTC 3 cut(s) 476, 614, 982
MmeI TCCRAC 1 cut(s) 341
MseI TTAA 1 cut(s) 387
MspR9I CCNGG 1 cut(s) 232
MvaI CCWGG 1 cut(s) 232
MwoI GCNNNNNNNGC 2 cut(s) 962, 1135
NmuCI GTSAC 2 cut(s) 644, 985
PagI TCATGA 2 cut(s) 660, 1023
PfeI GAWTC 6 cut(s) 329, 362, 425, 563, 746, 908
PflFI GACNNNGTC 1 cut(s) 350
PkrI GCNGC 1 cut(s) 964
PleI GAGTC 3 cut(s) 475, 613, 982
PmaCI CACGTG 1 cut(s) 199
PmlI CACGTG 1 cut(s) 199
PpsI GAGTC 3 cut(s) 475, 613, 982
Ppu21I YACGTR 1 cut(s) 199
Psp124BI GAGCTC 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 230
PspCI CACGTG 1 cut(s) 199
PspEI GGTNACC 1 cut(s) 644
PspGI CCWGG 1 cut(s) 230
PsyI GACNNNGTC 1 cut(s) 350
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SacI GAGCTC 1 cut(s) 1017
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 1 cut(s) 963
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 3 cut(s) 476, 614, 982
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 1 cut(s) 1017
SfaNI GCATC 1 cut(s) 1098
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
Sse9I AATT 7 cut(s) 56, 130, 146, 151, 189, 703, 917
SspMI CTAG 4 cut(s) 158, 366, 825, 1133
SstI GAGCTC 1 cut(s) 1017
StyD4I CCNGG 1 cut(s) 230
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 2 cut(s) 95, 265
TaiI ACGT 1 cut(s) 201
TaqI TCGA 1 cut(s) 753
TasI AATT 7 cut(s) 56, 130, 146, 151, 189, 703, 917
TatI WGTACW 1 cut(s) 95
TfiI GAWTC 6 cut(s) 329, 362, 425, 563, 746, 908
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 2 cut(s) 644, 985
TseI GCWGC 1 cut(s) 962
Tsp45I GTSAC 2 cut(s) 644, 985
TspRI CASTG 1 cut(s) 807
Tth111I GACNNNGTC 1 cut(s) 350
XapI RAATTY 5 cut(s) 56, 130, 146, 189, 917
XbaI TCTAGA 1 cut(s) 1132
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XspI CTAG 4 cut(s) 158, 366, 825, 1133
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.