Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007370

Physical Location & Seq

Genomic Coordinates Reverse (-)

22025035 .. 22030099

Gene Structure 5065 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G54250.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGGCCACAGAACAAGAATTCACACGCGCATCACGCTTCTCACGTGACTCAAGCAGCGTTGGATATTACTCAGAAGAAGATAACACGGAGGAGGAAGATGAAGAAGAAGAAGAGATGGAAGAAATCGAAGAAGAGGAGGAAGAAGAGGAAGAAGAAGACCCACGTATAGGGCTCACGTGCGGAGGAAGAAGAAACGGGTCAAGTAATAACAACAAATGGATGATGTTGGGTCGAATACTTGACCCGAGATCCAAATGGGTTCGAGAATGGAACAAAGTCTTTCTCCTTGTATGCGCAACGGGTCTATTCGTAGACCCACTTTTTCTCTATACCCTATCAGTGAGCGACACGTGTATGTGTCTCCTCGTCGACGGTTGGCTTGCTCTTACCGTCACTGCCTTACGCTCCATGACCGATCTTTTGCACCTTTGGAACATTTGGATTCAGTTCAAGATTGCTCGCCGGTGGCCTTATCCCGGCGGAGATAGCGACGGCGATACCAACAAAGGAGGAGGGACACGTGGCTCTACGAGGGTTGCTCCACCTTACGTTAAGAAGAATGGGTTCTTCTTCGATCTCTTCGTCATATTACCATTACCTCAGGTGGTGTTGTGGGTAGTGATACCTTCTCTTCTAAAGAGAGGCTCTGTGACTTTAGTGGTGTCAGTTTTGCTTGTAACATTCCTCTTCCAATATCTACCGAAAATATATCACTCAATCCGCCATCTCCGCCGCAATGCTACTCTCTCGGGTTACATCTTCGGCACCGTTTGGTGGGGCATTGCACTCAACATGATCGCTTATTTCGTCGCGGCTCATGCGGCAGGAGCGTGTTGGTACTTGTTAGGAGTTCAAAGGTCAGCGAAATGTCTTAAGGAACAATGTGAAAACACGATAGGATGCGACTTAAGGATGTTGTCATGTAAGGAACCGGTGTACTATGGAACGACCGTGATGGTCCTTGACAGAGCTAGACTTGCTTGGGCACAAAACCATCAAGCTCGATCTGTTTGCTTAGATATTAACACCAATTACACATATGGTGCTTATCAATGGACCATTCAACTTGTGAGCAGTGAGAGTCGGTTGGAGAAAATCCTTTTTCCTATCTTTTGGGGTCTCATGACTCTCAGCACATTTGGGAATTTGGAGAGCACAACAGAATGGTCGGAGGTTGTCTTCAATATCATAGTTCTAACAAGTGGTCTTCTTCTCGTTACCATGTTGATTGGTAACATCAAGGTATTTCTGCACGCTACAACTTCAAAGAAGCAAGCAATGCACTTGAAAATGAGGAACATAGAGTGGTGGATGAAGAAGAGACATTTGCCAATAGGGTTTAGACAGCGAGTCCGCAACTATGAGCGGCAAAGATGGGCTGCTATGCGCGGTGTAGACGAGTGTGAAATGGTTCAGAACCTTCCAGAAGGTCTTAGGAGAGACATCAAGTACCACCTCTGTCTAGACTTAGTCCGGCAGGTTCCATTGTTTCAGCATATGGATGATTTGGTGCTTGAGAATATATGCGACCGTGTGAAGTCTCTCATTTTCACCAAAGGAGAAACAATTCAGAAAGAAGGAGATGCAGTACAGAGAATGTTATTCGTAGTGAGAGGTCATCTTCAAAGCAGTCAGTTACTAAGAGACGGTGTCAAGAGCTGTTGCATGTTAGGTCCGGGAAATTTCAGCGGCGACGAGCTTCTCTCGTGGTGTCTCCGACGACCCTTCGTGGAGAGGCTACCGCCTTCTTCTTCAACGCTTGTAACACTCGAGACCACCGAAGCGTTTGGCCTCGACGCCGAAGACGTTAAGTACGTGACGCAGCATTTTCGTTACACTTTTGTTAACGAGAAAGTCAAACGTAGTGCTCGCTATTATTCTCCCGGATGGCGGACTTGGGCCGCGGTTGCGGTTCAGCTAGCTTGGCGAAGGTACAAGCATAGGCTAACGTTGACATCACTGTCGTTCATAAGGCCGAGAAGACCATTGTCAAGATGTGCGTCCTTAGGAGAAGACAAATTGAGGCTCTATGCAGCAATATTAACCTCTCCTAAACCCAATCCCGACGATTTTGATGATTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008219 GO:0008324 GO:0009626 GO:0009987 GO:0012501 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0033554 GO:0034050 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

694

Amino Acids

80.08

Weight (kDa)

8.2

Isoelectric Point (pI)

56.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	183 - 413	1.8e-06	Ion transport protein
cNMP_binding	PF00027	512 - 599	3.9e-06	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007370)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54250 AT5G54250 AT5G54250 AT5G54250
fragaria_vesca	FvH4_3g21060
malus_domestica	MD03G1218000.v1.1 MD11G1235800.v1.1
prunus_persica	Prupe.4G191200_v2.0.a1
pyrus_communis	pycom03g16750 pycom1049g00060 pycom1049g00070 pycom11g20730
rosa_chinensis	RchiOBHm_Chr5g0036081 RchiOBHm_Chr5g0036091
rosa_laevigata	RLG00000033664
rosa_multiflora	Rmu_sc0000382.1_g000031
rosa_roxburghii	Rroxscaffold_1G00044670
rosa_rugosa	Rorug05G0152600 Rorug05G0152600 Rorug05G0152600
rosa_samantha	Rh5AG246800 Rh5BG247100 Rh5CG278200 Rh5DG255700
rosa_wichuraiana	Rw5G022650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1960
Acc16I	TGCGCA	1 cut(s)	295
Acc36I	ACCTGC	1 cut(s)	1468
AccB1I	GGYRCC	1 cut(s)	764
AccBSI	CCGCTC	1 cut(s)	1366
AccI	GTMKAC	3 cut(s)	312, 369, 1395
AccII	CGCG	4 cut(s)	27, 812, 1389, 1904
AclI	AACGTT	1 cut(s)	1949
AclWI	GGATC	1 cut(s)	243
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	3 cut(s)	17, 1144, 1681
AcvI	CACGTG	4 cut(s)	44, 177, 351, 521
AcyI	GRCGYC	1 cut(s)	1797
AfaI	GTAC	6 cut(s)	839, 938, 1451, 1590, 1814, 1934
AfiI	CCNNNNNNNGG	4 cut(s)	167, 476, 774, 1890
AflII	CTTAAG	2 cut(s)	872, 907
AflIII	ACRYGT	3 cut(s)	348, 350, 518
AgeI	ACCGGT	1 cut(s)	931
AgsI	TTSAA	8 cut(s)	451, 854, 1064, 1183, 1266, 1288, 1625, 1755
AhdI	GACNNNNNGTC	1 cut(s)	1349
AluBI	AGCT	6 cut(s)	971, 1001, 1659, 1699, 1918, 1922
AluI	AGCT	6 cut(s)	971, 1001, 1659, 1699, 1918, 1922
Alw21I	GWGCWC	2 cut(s)	1157, 1870
Alw26I	GTCTC	8 cut(s)	365, 1124, 1315, 1434, 1545, 1638, 1718, 1766
AlwI	GGATC	1 cut(s)	243
Ama87I	CYCGRG	3 cut(s)	244, 748, 1769
AoxI	GGCC	5 cut(s)	3, 467, 1789, 1899, 1973
ApeKI	GCWGC	4 cut(s)	54, 1379, 1822, 2033
ApoI	RAATTY	3 cut(s)	17, 1144, 1681
ArsI	GACNNNNNNTTYG	2 cut(s)	403, 435
AsiGI	ACCGGT	1 cut(s)	931
Asp700I	GAANNNNTTC	3 cut(s)	563, 1410, 1566
AspLEI	GCGC	3 cut(s)	29, 296, 1389
AspS9I	GGNCC	4 cut(s)	958, 1056, 1673, 1899
AsuC2I	CCSGG	3 cut(s)	477, 1677, 1884
AsuHPI	GGTGA	1 cut(s)	1543
AsuNHI	GCTAGC	1 cut(s)	1918
AvaI	CYCGRG	3 cut(s)	244, 748, 1769
AvaII	GGWCC	3 cut(s)	958, 1056, 1673
AxyI	CCTNAGG	2 cut(s)	600, 2005
BaeGI	GKGCMC	1 cut(s)	988
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	764
BanII	GRGCYC	1 cut(s)	174
BauI	CACGAG	1 cut(s)	1705
BbrPI	CACGTG	4 cut(s)	44, 177, 351, 521
BbsI	GAAGAC	6 cut(s)	162, 1171, 1199, 1809, 1987, 2019
Bbv12I	GWGCWC	2 cut(s)	1157, 1870
BbvI	GCAGC	4 cut(s)	66, 1366, 1834, 2045
BccI	CCATC	6 cut(s)	109, 732, 949, 1002, 1368, 1881
BceAI	ACGGC	1 cut(s)	508
BcgI	CGANNNNNNTGC	4 cut(s)	993, 1027, 1811, 1845
BcnI	CCSGG	3 cut(s)	477, 1677, 1884
BcoDI	GTCTC	8 cut(s)	365, 1124, 1315, 1434, 1545, 1638, 1718, 1766
BfaI	CTAG	3 cut(s)	972, 1463, 1919
BfrI	CTTAAG	2 cut(s)	872, 907
BfuAI	ACCTGC	1 cut(s)	1468
Bme1390I	CCNGG	3 cut(s)	477, 1677, 1884
Bme18I	GGWCC	3 cut(s)	958, 1056, 1673
BmeRI	GACNNNNNGTC	1 cut(s)	1349
BmeT110I	CYCGRG	3 cut(s)	244, 748, 1769
BmgT120I	GGNCC	4 cut(s)	958, 1056, 1673, 1899
BmiI	GGNNCC	3 cut(s)	766, 930, 1482
BmrFI	CCNGG	3 cut(s)	477, 1677, 1884
BmsI	GCATC	3 cut(s)	38, 890, 1573
BmtI	GCTAGC	1 cut(s)	1922
BoxI	GACNNNNGTC	1 cut(s)	1987
BpiI	GAAGAC	6 cut(s)	162, 1171, 1199, 1809, 1987, 2019
BplI	GAGNNNNNCTC	4 cut(s)	523, 555, 1688, 1720
BpuEI	CTTGAG	2 cut(s)	34, 1535
BpuMI	CCSGG	3 cut(s)	477, 1677, 1884
BsaAI	YACGTR	6 cut(s)	44, 164, 177, 351, 521, 1816
BsaHI	GRCGYC	1 cut(s)	1797
BsaI	GGTCTC	2 cut(s)	1124, 1766
BsaJI	CCNNGG	1 cut(s)	1902
BsaWI	WCCGGW	1 cut(s)	931
BsaXI	ACNNNNNCTCC	2 cut(s)	1572, 1602
Bsc4I	CCNNNNNNNGG	4 cut(s)	167, 476, 774, 1890
Bse118I	RCCGGY	2 cut(s)	462, 931
Bse21I	CCTNAGG	2 cut(s)	600, 2005
Bse3DI	GCAATG	3 cut(s)	742, 780, 1284
BseDI	CCNNGG	1 cut(s)	1902
BseGI	GGATG	6 cut(s)	225, 905, 918, 1317, 1507, 1892
BseLI	CCNNNNNNNGG	4 cut(s)	167, 476, 774, 1890
BseMI	GCAATG	3 cut(s)	742, 780, 1284
BseMII	CTCAG	3 cut(s)	84, 614, 1144
BseRI	GAGGAG	4 cut(s)	104, 149, 353, 525
BseSI	GKGCMC	1 cut(s)	988
BseXI	GCAGC	4 cut(s)	66, 1366, 1834, 2045
BsgI	GTGCAG	1 cut(s)	1235
Bsh1236I	CGCG	4 cut(s)	27, 812, 1389, 1904
Bsh1285I	CGRYCG	2 cut(s)	951, 1531
BshFI	GGCC	5 cut(s)	5, 469, 1791, 1901, 1975
BshNI	GGYRCC	1 cut(s)	764
BshTI	ACCGGT	1 cut(s)	931
BsiEI	CGRYCG	2 cut(s)	951, 1531
BsiHKAI	GWGCWC	2 cut(s)	1157, 1870
BsiHKCI	CYCGRG	3 cut(s)	244, 748, 1769
BsiSI	CCGG	6 cut(s)	463, 477, 932, 1474, 1676, 1884
BslFI	GGGAC	1 cut(s)	529
BslI	CCNNNNNNNGG	4 cut(s)	167, 476, 774, 1890
BsmAI	GTCTC	8 cut(s)	365, 1124, 1315, 1434, 1545, 1638, 1718, 1766
BsmBI	CGTCTC	1 cut(s)	1638
BsmFI	GGGAC	1 cut(s)	529
BsnI	GGCC	5 cut(s)	5, 469, 1791, 1901, 1975
Bso31I	GGTCTC	2 cut(s)	1124, 1766
BsoBI	CYCGRG	3 cut(s)	244, 748, 1769
Bsp1286I	GDGCHC	4 cut(s)	174, 988, 1157, 1870
Bsp143I	GATC	5 cut(s)	248, 415, 574, 795, 1004
BspANI	GGCC	5 cut(s)	5, 469, 1791, 1901, 1975
BspCNI	CTCAG	3 cut(s)	83, 613, 1143
BspFNI	CGCG	4 cut(s)	27, 812, 1389, 1904
BspHI	TCATGA	1 cut(s)	1122
BspLI	GGNNCC	3 cut(s)	766, 930, 1482
BspMI	ACCTGC	1 cut(s)	1468
BspOI	GCTAGC	1 cut(s)	1922
BspPI	GGATC	1 cut(s)	243
BspT107I	GGYRCC	1 cut(s)	764
BspTI	CTTAAG	2 cut(s)	872, 907
BspTNI	GGTCTC	2 cut(s)	1124, 1766
BsrBI	CCGCTC	1 cut(s)	1366
BsrDI	GCAATG	3 cut(s)	742, 780, 1284
BsrFI	RCCGGY	2 cut(s)	462, 931
BssAI	RCCGGY	2 cut(s)	462, 931
BssECI	CCNNGG	1 cut(s)	1902
BssMI	GATC	5 cut(s)	248, 415, 574, 795, 1004
BssNI	GRCGYC	1 cut(s)	1797
BssSI	CACGAG	1 cut(s)	1705
Bst2BI	CACGAG	1 cut(s)	1705
Bst4CI	ACNGT	7 cut(s)	374, 391, 769, 952, 1532, 1649, 1962
Bst6I	CTCTTC	7 cut(s)	105, 126, 138, 584, 636, 692, 1313
BstACI	GRCGYC	1 cut(s)	1797
BstAFI	CTTAAG	2 cut(s)	872, 907
BstAPI	GCANNNNNTGC	1 cut(s)	1279
BstBAI	YACGTR	6 cut(s)	44, 164, 177, 351, 521, 1816
BstC8I	GCNNGC	6 cut(s)	381, 460, 1254, 1275, 1870, 1920
BstDEI	CTNAG	8 cut(s)	70, 600, 1015, 1130, 1433, 1468, 1640, 2005
BstDSI	CCRYGG	1 cut(s)	1902
BstF5I	GGATG	6 cut(s)	225, 905, 918, 1317, 1507, 1892
BstFNI	CGCG	4 cut(s)	27, 812, 1389, 1904
BstHHI	GCGC	3 cut(s)	29, 296, 1389
BstKTI	GATC	5 cut(s)	251, 418, 577, 798, 1007
BstMAI	GTCTC	8 cut(s)	365, 1124, 1315, 1434, 1545, 1638, 1718, 1766
BstMBI	GATC	5 cut(s)	248, 415, 574, 795, 1004
BstMCI	CGRYCG	2 cut(s)	951, 1531
BstNSI	RCATGY	1 cut(s)	1669
BstPAI	GACNNNNGTC	1 cut(s)	1987
BstSCI	CCNGG	3 cut(s)	475, 1675, 1882
BstSLI	GKGCMC	1 cut(s)	988
BstUI	CGCG	4 cut(s)	27, 812, 1389, 1904
BstV1I	GCAGC	4 cut(s)	66, 1366, 1834, 2045
BstV2I	GAAGAC	6 cut(s)	162, 1171, 1199, 1809, 1987, 2019
BstX2I	RGATCY	1 cut(s)	248
BstYI	RGATCY	1 cut(s)	248
Bsu36I	CCTNAGG	2 cut(s)	600, 2005
BsuRI	GGCC	5 cut(s)	5, 469, 1791, 1901, 1975
BtgI	CCRYGG	1 cut(s)	1902
BtsCI	GGATG	6 cut(s)	225, 905, 918, 1317, 1507, 1892
BtsI	GCAGTG	2 cut(s)	393, 1081
BtsIMutI	CAGTG	4 cut(s)	345, 393, 1081, 1958
BveI	ACCTGC	1 cut(s)	1468
Cac8I	GCNNGC	6 cut(s)	381, 460, 1254, 1275, 1870, 1920
CciI	TCATGA	1 cut(s)	1122
CfoI	GCGC	3 cut(s)	29, 296, 1389
Cfr10I	RCCGGY	2 cut(s)	462, 931
Cfr13I	GGNCC	4 cut(s)	958, 1056, 1673, 1899
Cfr42I	CCGCGG	1 cut(s)	1905
CseI	GACGC	3 cut(s)	1805, 1828, 1989
Csp6I	GTAC	6 cut(s)	838, 937, 1450, 1589, 1813, 1933
CspAI	ACCGGT	1 cut(s)	931
CviAII	CATG	7 cut(s)	409, 793, 818, 921, 1123, 1222, 1666
CviQI	GTAC	6 cut(s)	838, 937, 1450, 1589, 1813, 1933
DdeI	CTNAG	8 cut(s)	70, 600, 1015, 1130, 1433, 1468, 1640, 2005
DpnI	GATC	5 cut(s)	250, 417, 576, 797, 1006
DpnII	GATC	5 cut(s)	248, 415, 574, 795, 1004
DrdI	GACNNNNNNGTC	1 cut(s)	1960
DriI	GACNNNNNGTC	1 cut(s)	1349
DseDI	GACNNNNNNGTC	1 cut(s)	1960
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	7 cut(s)	105, 126, 138, 584, 636, 692, 1313
Eam1105I	GACNNNNNGTC	1 cut(s)	1349
EarI	CTCTTC	7 cut(s)	105, 126, 138, 584, 636, 692, 1313
EciI	GGCGGA	4 cut(s)	495, 710, 719, 1906
Eco24I	GRGCYC	1 cut(s)	174
Eco31I	GGTCTC	2 cut(s)	1124, 1766
Eco47I	GGWCC	3 cut(s)	958, 1056, 1673
Eco72I	CACGTG	4 cut(s)	44, 177, 351, 521
Eco81I	CCTNAGG	2 cut(s)	600, 2005
Eco88I	CYCGRG	3 cut(s)	244, 748, 1769
EcoRI	GAATTC	1 cut(s)	17
EcoT38I	GRGCYC	1 cut(s)	174
Esp3I	CGTCTC	1 cut(s)	1638
FaeI	CATG	7 cut(s)	412, 796, 821, 924, 1126, 1225, 1669
FaqI	GGGAC	1 cut(s)	529
FatI	CATG	7 cut(s)	408, 792, 817, 920, 1122, 1221, 1665
FauNDI	CATATG	2 cut(s)	1039, 1497
FblI	GTMKAC	3 cut(s)	312, 369, 1395
FokI	GGATG	6 cut(s)	232, 912, 925, 1324, 1514, 1899
FriOI	GRGCYC	1 cut(s)	174
FspBI	CTAG	3 cut(s)	972, 1463, 1919
FspI	TGCGCA	1 cut(s)	295
GlaI	GCGC	3 cut(s)	28, 295, 1388
HaeIII	GGCC	5 cut(s)	5, 469, 1791, 1901, 1975
HapII	CCGG	6 cut(s)	463, 477, 932, 1474, 1676, 1884
HgaI	GACGC	3 cut(s)	1805, 1828, 1989
HhaI	GCGC	3 cut(s)	29, 296, 1389
Hin1I	GRCGYC	1 cut(s)	1797
Hin1II	CATG	7 cut(s)	412, 796, 821, 924, 1126, 1225, 1669
Hin6I	GCGC	3 cut(s)	27, 294, 1387
HinP1I	GCGC	3 cut(s)	27, 294, 1387
HincII	GTYRAC	3 cut(s)	370, 1846, 1953
HindII	GTYRAC	3 cut(s)	370, 1846, 1953
HinfI	GANTC	5 cut(s)	47, 442, 1081, 1126, 1350
HpaI	GTTAAC	1 cut(s)	1846
HpaII	CCGG	6 cut(s)	463, 477, 932, 1474, 1676, 1884
HphI	GGTGA	1 cut(s)	1543
Hpy166II	GTNNAC	6 cut(s)	313, 370, 937, 1396, 1846, 1953
Hpy188I	TCNGA	5 cut(s)	73, 1171, 1416, 1572, 1718
Hpy188III	TCNNGA	9 cut(s)	263, 451, 1123, 1424, 1463, 1654, 1771, 1992, 2063
Hpy8I	GTNNAC	6 cut(s)	313, 370, 937, 1396, 1846, 1953
Hpy99I	CGWCG	8 cut(s)	371, 374, 494, 812, 1697, 1722, 1799, 2069
HpyAV	CCTTC	7 cut(s)	636, 1421, 1430, 1571, 1735, 1755, 1923
HpyCH4III	ACNGT	7 cut(s)	374, 391, 769, 952, 1532, 1649, 1962
HpyCH4V	TGCA	7 cut(s)	424, 785, 1252, 1282, 1586, 1665, 2033
HpyF3I	CTNAG	8 cut(s)	70, 600, 1015, 1130, 1433, 1468, 1640, 2005
Hsp92I	GRCGYC	1 cut(s)	1797
Hsp92II	CATG	7 cut(s)	412, 796, 821, 924, 1126, 1225, 1669
HspAI	GCGC	3 cut(s)	27, 294, 1387
KspAI	GTTAAC	1 cut(s)	1846
KspI	CCGCGG	1 cut(s)	1905
Kzo9I	GATC	5 cut(s)	248, 415, 574, 795, 1004
LmnI	GCTCC	3 cut(s)	410, 544, 827
Lsp1109I	GCAGC	4 cut(s)	66, 1366, 1834, 2045
LweI	GCATC	3 cut(s)	38, 890, 1573
MaeI	CTAG	3 cut(s)	972, 1463, 1919
MalI	GATC	5 cut(s)	250, 417, 576, 797, 1006
MbiI	CCGCTC	1 cut(s)	1366
MboI	GATC	5 cut(s)	248, 415, 574, 795, 1004
MflI	RGATCY	1 cut(s)	248
MhlI	GDGCHC	4 cut(s)	174, 988, 1157, 1870
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	6 cut(s)	17, 1030, 1144, 1566, 1681, 2018
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	4 cut(s)	41, 1090, 1120, 1359
MmeI	TCCRAC	4 cut(s)	40, 1068, 1149, 1741
Mox20I	TGGCCA	1 cut(s)	5
MroXI	GAANNNNTTC	3 cut(s)	563, 1410, 1566
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	7 cut(s)	552, 873, 908, 1023, 1809, 1845, 2042
MslI	CAYNNNNRTG	2 cut(s)	353, 1500
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	1689, 1904
MspCI	CTTAAG	2 cut(s)	872, 907
MspI	CCGG	6 cut(s)	463, 477, 932, 1474, 1676, 1884
MspR9I	CCNGG	3 cut(s)	477, 1677, 1884
MvnI	CGCG	4 cut(s)	27, 812, 1389, 1904
NciI	CCSGG	3 cut(s)	477, 1677, 1884
NdeI	CATATG	2 cut(s)	1039, 1497
NdeII	GATC	5 cut(s)	248, 415, 574, 795, 1004
NheI	GCTAGC	1 cut(s)	1918
NlaIII	CATG	7 cut(s)	412, 796, 821, 924, 1126, 1225, 1669
NlaIV	GGNNCC	3 cut(s)	766, 930, 1482
NmeAIII	GCCGAG	1 cut(s)	2001
NmuCI	GTSAC	4 cut(s)	44, 391, 649, 1816
NsbI	TGCGCA	1 cut(s)	295
NspI	RCATGY	1 cut(s)	1669
PaeR7I	CTCGAG	1 cut(s)	1769
PagI	TCATGA	1 cut(s)	1122
PcsI	WCGNNNNNNNCGW	6 cut(s)	40, 579, 804, 1776, 1803, 1812
PdmI	GAANNNNTTC	3 cut(s)	563, 1410, 1566
PfeI	GAWTC	1 cut(s)	442
PflFI	GACNNNGTC	2 cut(s)	1469, 1649
PfoI	TCCNGGA	2 cut(s)	1675, 1882
PinAI	ACCGGT	1 cut(s)	931
PleI	GAGTC	4 cut(s)	41, 1089, 1120, 1358
PmaCI	CACGTG	4 cut(s)	44, 177, 351, 521
PmlI	CACGTG	4 cut(s)	44, 177, 351, 521
PpsI	GAGTC	4 cut(s)	41, 1089, 1120, 1358
Ppu21I	YACGTR	6 cut(s)	44, 164, 177, 351, 521, 1816
PshAI	GACNNNNGTC	1 cut(s)	1987
Psp1406I	AACGTT	1 cut(s)	1949
PspCI	CACGTG	4 cut(s)	44, 177, 351, 521
PspN4I	GGNNCC	3 cut(s)	766, 930, 1482
PspPI	GGNCC	4 cut(s)	958, 1056, 1673, 1899
PsuI	RGATCY	1 cut(s)	248
PsyI	GACNNNGTC	2 cut(s)	1469, 1649
RsaI	GTAC	6 cut(s)	839, 938, 1451, 1590, 1814, 1934
RsaNI	GTAC	6 cut(s)	838, 937, 1450, 1589, 1813, 1933
RseI	CAYNNNNRTG	2 cut(s)	353, 1500
SacII	CCGCGG	1 cut(s)	1905
SalI	GTCGAC	1 cut(s)	368
SaqAI	TTAA	7 cut(s)	552, 873, 908, 1023, 1809, 1845, 2042
Sau3AI	GATC	5 cut(s)	248, 415, 574, 795, 1004
Sau96I	GGNCC	4 cut(s)	958, 1056, 1673, 1899
SchI	GAGTC	4 cut(s)	41, 1090, 1120, 1359
ScrFI	CCNGG	3 cut(s)	477, 1677, 1884
SduI	GDGCHC	4 cut(s)	174, 988, 1157, 1870
SfaNI	GCATC	3 cut(s)	38, 890, 1573
Sfr274I	CTCGAG	1 cut(s)	1769
Sfr303I	CCGCGG	1 cut(s)	1905
SgrAI	CRCCGGYG	1 cut(s)	462
SgrBI	CCGCGG	1 cut(s)	1905
SgrDI	CGTCGACG	1 cut(s)	368
SinI	GGWCC	3 cut(s)	958, 1056, 1673
SlaI	CTCGAG	1 cut(s)	1769
SmiMI	CAYNNNNRTG	2 cut(s)	353, 1500
SmlI	CTYRAG	5 cut(s)	49, 872, 907, 1514, 1769
SmoI	CTYRAG	5 cut(s)	49, 872, 907, 1514, 1769
Sse9I	AATT	6 cut(s)	17, 1030, 1144, 1566, 1681, 2018
SspI	AATATT	1 cut(s)	2040
SspMI	CTAG	3 cut(s)	972, 1463, 1919
StyD4I	CCNGG	3 cut(s)	475, 1675, 1882
TaaI	ACNGT	7 cut(s)	374, 391, 769, 952, 1532, 1649, 1962
TaqI	TCGA	8 cut(s)	126, 232, 262, 369, 573, 1003, 1770, 1794
TaqII	GACCGA	1 cut(s)	428
TasI	AATT	6 cut(s)	17, 1030, 1144, 1566, 1681, 2018
TatI	WGTACW	2 cut(s)	936, 1588
TauI	GCSGC	6 cut(s)	735, 815, 824, 1369, 1692, 1904
TfiI	GAWTC	1 cut(s)	442
Tru1I	TTAA	7 cut(s)	552, 873, 908, 1023, 1809, 1845, 2042
Tru9I	TTAA	7 cut(s)	552, 873, 908, 1023, 1809, 1845, 2042
TscAI	CASTG	4 cut(s)	345, 400, 1081, 1965
TseFI	GTSAC	4 cut(s)	44, 391, 649, 1816
TseI	GCWGC	4 cut(s)	54, 1379, 1822, 2033
Tsp45I	GTSAC	4 cut(s)	44, 391, 649, 1816
TspDTI	ATGAA	3 cut(s)	114, 1328, 1957
TspGWI	ACGGA	1 cut(s)	101
TspRI	CASTG	4 cut(s)	345, 400, 1081, 1965
Tth111I	GACNNNGTC	2 cut(s)	1469, 1649
Vha464I	CTTAAG	2 cut(s)	872, 907
VpaK11BI	GGWCC	3 cut(s)	958, 1056, 1673
XapI	RAATTY	3 cut(s)	17, 1144, 1681
XbaI	TCTAGA	1 cut(s)	1462
XceI	RCATGY	1 cut(s)	1669
XhoI	CTCGAG	1 cut(s)	1769
XmiI	GTMKAC	3 cut(s)	312, 369, 1395
XmnI	GAANNNNTTC	3 cut(s)	563, 1410, 1566
XspI	CTAG	3 cut(s)	972, 1463, 1919

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.