AT5G55710

Protein TIC 20-v, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010435
Physical Location & Seq
Reverse (-)
22554878 .. 22555890
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55710.1

Sequence Viewer

Length: 630 bp
ATGGCAATAATATCTCAGTTTTTTGCTCCATTACCATCTCTCACCGGAACCCTAACCCTAACCGGTCGTTCTTTTCTCCCTCTAAACCTTGATACCCAATTCCCAAAACCTAGACTTTCCAGGGACAGAGCCGCCACACTCGTACTCCAATCCAAGGGTGATGATTCCGTCGATGCATCGGATCGGATAATCTCAGCCGTCTGTTACTTCTACCCATTCTTCGATGGGATTCAATACGGCAAATTCATCATCACACAGTACCAACCTTTTCAGATTCTCATCCAACCTTTGTTTCCCGCCATCAGAGCTTTCAAGAGCTTCCCTTTCAATGGGTTTCTTATCTTCATCACTCTCTACTTCGTCGTTGTGAGGAACCCTAATTTCAGCAGGTACGTCAGGTTCAACACGATGCAGGCGATTGTGCTTGATGTGTTGTTGATTTTCCCGGATTTGCTTGAGAGGAGCTTTAATCCTAGAGATGGGTTTGGTTTAGATGTGGTGATGAGTTTGGACAGCACAGTGTTCCTCTTCTTGCTTGTCTCTTTGATCTATGGATTCTCTGCTTGCTTGTTTGGTCTGACCCCGAGGTTGCCTCTTGTTGCCGAGGCTGCTGATAGGCAAGTCCTTTGA

Protein Analysis

209

Amino Acids

23.65

Weight (kDa)

8.61

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 41 - 204 1.6e-55 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 378
AciI CCGC 2 cut(s) 132, 297
AclWI GGATC 1 cut(s) 189
AcsI RAATTY 1 cut(s) 242
AfaI GTAC 3 cut(s) 144, 260, 392
AfiI CCNNNNNNNGG 3 cut(s) 154, 329, 479
AgeI ACCGGT 1 cut(s) 62
AgsI TTSAA 4 cut(s) 233, 313, 328, 403
AjnI CCWGG 1 cut(s) 119
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AluBI AGCT 3 cut(s) 308, 318, 465
AluI AGCT 3 cut(s) 308, 318, 465
Alw26I GTCTC 1 cut(s) 544
AlwI GGATC 1 cut(s) 189
Ama87I CYCGRG 1 cut(s) 583
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 1 cut(s) 242
AsiGI ACCGGT 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 3 cut(s) 34, 170, 511
AvaI CYCGRG 1 cut(s) 583
BbvI GCAGC 1 cut(s) 595
BccI CCATC 4 cut(s) 43, 218, 308, 473
BceAI ACGGC 2 cut(s) 182, 253
BciT130I CCWGG 1 cut(s) 121
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 2 cut(s) 111, 474
BfuAI ACCTGC 1 cut(s) 378
BisI GCNGC 2 cut(s) 132, 609
BlsI GCNGC 2 cut(s) 133, 610
Bme1390I CCNGG 2 cut(s) 121, 446
BmeT110I CYCGRG 1 cut(s) 583
BmiI GGNNCC 2 cut(s) 49, 374
BmrFI CCNGG 2 cut(s) 121, 446
BmsI GCATC 3 cut(s) 163, 185, 399
BplI GAGNNNNNCTC 2 cut(s) 577, 609
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 446
BsaBI GATNNNNATC 1 cut(s) 278
BsaJI CCNNGG 4 cut(s) 120, 153, 584, 603
BsaWI WCCGGW 2 cut(s) 44, 62
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 329, 479
Bse118I RCCGGY 1 cut(s) 62
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 4 cut(s) 120, 153, 584, 603
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 3 cut(s) 154, 329, 479
BseMII CTCAG 2 cut(s) 29, 207
BseRI GAGGAG 1 cut(s) 475
BseXI GCAGC 1 cut(s) 595
Bsh1285I CGRYCG 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 583
BsiSI CCGG 3 cut(s) 45, 63, 446
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 3 cut(s) 154, 329, 479
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 1 cut(s) 137
BsoBI CYCGRG 1 cut(s) 583
Bsp143I GATC 2 cut(s) 181, 546
BspACI CCGC 2 cut(s) 132, 297
BspCNI CTCAG 2 cut(s) 28, 206
BspLI GGNNCC 2 cut(s) 49, 374
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 62
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 4 cut(s) 120, 153, 584, 603
BssMI GATC 2 cut(s) 181, 546
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 2 cut(s) 258, 520
Bst6I CTCTTC 1 cut(s) 533
BstC8I GCNNGC 2 cut(s) 414, 565
BstDEI CTNAG 2 cut(s) 15, 193
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 2 cut(s) 184, 549
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 2 cut(s) 181, 546
BstMCI CGRYCG 1 cut(s) 67
BstMWI GCNNNNNNNGC 2 cut(s) 305, 608
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 2 cut(s) 119, 444
BstV1I GCAGC 1 cut(s) 595
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 525
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 2 cut(s) 414, 565
Cfr10I RCCGGY 1 cut(s) 62
Csp6I GTAC 3 cut(s) 143, 259, 391
CspAI ACCGGT 1 cut(s) 62
CviJI RGCY 6 cut(s) 131, 197, 308, 318, 465, 608
CviKI_1 RGCY 6 cut(s) 131, 197, 308, 318, 465, 608
CviQI GTAC 3 cut(s) 143, 259, 391
DdeI CTNAG 2 cut(s) 15, 193
DpnI GATC 2 cut(s) 183, 548
DpnII GATC 2 cut(s) 181, 546
Eam1104I CTCTTC 1 cut(s) 533
EarI CTCTTC 1 cut(s) 533
Eco130I CCWWGG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 153
EcoT22I ATGCAT 1 cut(s) 178
ErhI CCWWGG 1 cut(s) 153
FaiI YATR 1 cut(s) 552
FaqI GGGAC 1 cut(s) 137
FauI CCCGC 1 cut(s) 304
Fnu4HI GCNGC 2 cut(s) 132, 609
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 132, 609
FspBI CTAG 2 cut(s) 111, 474
GluI GCNGC 2 cut(s) 132, 609
HapII CCGG 3 cut(s) 45, 63, 446
HinfI GANTC 4 cut(s) 164, 229, 274, 555
HpaII CCGG 3 cut(s) 45, 63, 446
HphI GGTGA 3 cut(s) 34, 170, 511
Hpy188I TCNGA 5 cut(s) 181, 186, 273, 305, 579
Hpy188III TCNNGA 1 cut(s) 313
Hpy99I CGWCG 2 cut(s) 173, 365
HpyCH4III ACNGT 2 cut(s) 258, 520
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 2 cut(s) 176, 412
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 608
HpyF3I CTNAG 2 cut(s) 15, 193
HpySE526I ACGT 1 cut(s) 393
Kzo9I GATC 2 cut(s) 181, 546
LmnI GCTCC 2 cut(s) 31, 462
LpnPI CCDG 8 cut(s) 58, 76, 106, 133, 373, 382, 398, 459
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 3 cut(s) 163, 185, 399
MaeI CTAG 2 cut(s) 111, 474
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 203
MalI GATC 2 cut(s) 183, 548
MboI GATC 2 cut(s) 181, 546
MboII GAAGA 3 cut(s) 211, 334, 520
MluCI AATT 3 cut(s) 98, 242, 379
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 7 cut(s) 90, 363, 453, 536, 579, 598, 603
Mph1103I ATGCAT 1 cut(s) 178
MseI TTAA 1 cut(s) 468
MspI CCGG 3 cut(s) 45, 63, 446
MspR9I CCNGG 2 cut(s) 121, 446
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 2 cut(s) 305, 608
NciI CCSGG 1 cut(s) 446
NdeII GATC 2 cut(s) 181, 546
NlaIV GGNNCC 2 cut(s) 49, 374
NmeAIII GCCGAG 1 cut(s) 628
NsiI ATGCAT 1 cut(s) 178
PcsI WCGNNNNNNNCGW 1 cut(s) 413
PfeI GAWTC 4 cut(s) 164, 229, 274, 555
PfoI TCCNGGA 1 cut(s) 444
PinAI ACCGGT 1 cut(s) 62
PkrI GCNGC 2 cut(s) 133, 610
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 49, 374
PsrI GAACNNNNNNTAC 2 cut(s) 383, 415
RsaI GTAC 3 cut(s) 144, 260, 392
RsaNI GTAC 3 cut(s) 143, 259, 391
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 2 cut(s) 132, 609
Sau3AI GATC 2 cut(s) 181, 546
ScrFI CCNGG 2 cut(s) 121, 446
SfaNI GCATC 3 cut(s) 163, 185, 399
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 3 cut(s) 98, 242, 379
SsiI CCGC 2 cut(s) 132, 297
SspMI CTAG 2 cut(s) 111, 474
StyD4I CCNGG 2 cut(s) 119, 444
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 2 cut(s) 258, 520
TaiI ACGT 1 cut(s) 396
TaqI TCGA 2 cut(s) 171, 222
TasI AATT 3 cut(s) 98, 242, 379
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 164, 229, 274, 555
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 1 cut(s) 525
TseI GCWGC 1 cut(s) 608
TspDTI ATGAA 2 cut(s) 235, 334
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 1 cut(s) 525
XapI RAATTY 1 cut(s) 242
XspI CTAG 2 cut(s) 111, 474
Zsp2I ATGCAT 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.