Rorug05G0279800

Chloroplast import apparatus Tic20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010435
Physical Location & Seq
Reverse (-)
31758414 .. 31770078
11665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0279800.1

Sequence Viewer

Length: 762 bp
ATGGGGGAAAGCAGTACTTACTATGATAAGAATGATGCACTGAAGAAAGGAGCCTGGGCTGCAAGTGAAGACCAGATTGTCAAAGACTACGTCGAAAAACACGGTGTCGGAAAATGGGGCAAACTATCTAGGGAAACAGGCAATTCAAGTTTAAATTCTTGTTTTCATTCACGTATGAAGAGATGTGCGAAGAGTGTGAGACTTAGGTGGCTGAACTATCTGAGGCCTGATATCATCAGAGGCAACATTAAAGAAGATGAAGAAGAGCTCATCATCAGGCTCCACAAACTCTTGGGAAACAGATGGTCATTGATAGCTGGAAGGATACCAGGGCGAACTGACAATGAAATCAAAAACTACTGGAATTCCACCTTAAAGAGGAAGCTACAACAAGAAAATCACTTAGAAGTGTCTAAAGATGAGGAGATCAAGGAGGACTGGTCATCCTCTGCCGTTGTTGCTGCTGCTGCTGATGAGGAAAACCCAGGTTTGACCAAAAAGAATGTCACTGAAGTTGGAGTTGGAGTTGGAGTTGGAGTTGAAGCAACTAATAATAACGGTATTGGTGATGATGAATATCTGAGGGATGACCTTTGGTGGAACAATTTCATAGCGGATTTCAATGATACCCAACTCGACGTATCTGACTTCCAAACCGACTTCCCTGAGAATTGGGAATCTAAGGCGAACATTATCGACGTCGGAGATTGCTTCACCGATCAACAAGACATGCCAAATAATATTGCTTGGGGTACATATTAA

Protein Analysis

253

Amino Acids

28.84

Weight (kDa)

4.9

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 16 - 74 1e-10 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 19 - 88 2.8e-07 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 80 - 124 1.5e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 85 - 133 3e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 702
AciI CCGC 1 cut(s) 614
AcsI RAATTY 2 cut(s) 154, 364
AcuI CTGAAG 2 cut(s) 62, 531
AcyI GRCGYC 1 cut(s) 699
AfaI GTAC 2 cut(s) 16, 754
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 3 cut(s) 147, 542, 622
AjnI CCWGG 3 cut(s) 53, 328, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
AluBI AGCT 3 cut(s) 268, 317, 385
AluI AGCT 3 cut(s) 268, 317, 385
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 193
AoxI GGCC 1 cut(s) 224
ApeKI GCWGC 4 cut(s) 59, 461, 464, 467
ApoI RAATTY 2 cut(s) 154, 364
ArsI GACNNNNNNTTYG 4 cut(s) 75, 107, 489, 521
Asp700I GAANNNNTTC 1 cut(s) 605
AsuHPI GGTGA 2 cut(s) 578, 706
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 4 cut(s) 46, 448, 451, 454
BccI CCATC 1 cut(s) 297
BceAI ACGGC 1 cut(s) 437
BciT130I CCWGG 3 cut(s) 55, 330, 486
BciVI GTATCC 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 129
BfuI GTATCC 1 cut(s) 318
BisI GCNGC 4 cut(s) 60, 462, 465, 468
BlsI GCNGC 4 cut(s) 61, 463, 466, 469
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 3 cut(s) 55, 330, 486
BmiI GGNNCC 2 cut(s) 52, 281
BmrFI CCNGG 3 cut(s) 55, 330, 486
BmsI GCATC 1 cut(s) 25
BpiI GAAGAC 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 699
BsaJI CCNNGG 3 cut(s) 54, 329, 484
BsaXI ACNNNNNCTCC 8 cut(s) 425, 455, 510, 516, 522, 540, 546, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse1I ACTGG 2 cut(s) 365, 443
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 3 cut(s) 55, 330, 486
BseDI CCNNGG 3 cut(s) 54, 329, 484
BseGI GGATG 2 cut(s) 443, 592
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 3 cut(s) 212, 572, 657
BseNI ACTGG 2 cut(s) 365, 443
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 4 cut(s) 46, 448, 451, 454
BshFI GGCC 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 1 cut(s) 193
BsnI GGCC 1 cut(s) 226
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 2 cut(s) 426, 718
BspACI CCGC 1 cut(s) 614
BspANI GGCC 1 cut(s) 226
BspCNI CTCAG 3 cut(s) 213, 573, 658
BspLI GGNNCC 2 cut(s) 52, 281
BspQI GCTCTTC 1 cut(s) 258
BsrI ACTGG 2 cut(s) 365, 443
BssECI CCNNGG 3 cut(s) 54, 329, 484
BssMI GATC 2 cut(s) 426, 718
BssNI GRCGYC 1 cut(s) 699
Bst2UI CCWGG 3 cut(s) 55, 330, 486
Bst4CI ACNGT 2 cut(s) 104, 560
Bst6I CTCTTC 3 cut(s) 173, 185, 258
BstACI GRCGYC 1 cut(s) 699
BstBAI YACGTR 1 cut(s) 173
BstDEI CTNAG 6 cut(s) 203, 221, 403, 581, 666, 681
BstENI CCTNNNNNAGG 1 cut(s) 376
BstF5I GGATG 2 cut(s) 443, 592
BstKTI GATC 2 cut(s) 429, 721
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 2 cut(s) 426, 718
BstMWI GCNNNNNNNGC 3 cut(s) 59, 458, 467
BstNI CCWGG 3 cut(s) 55, 330, 486
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 3 cut(s) 53, 328, 484
BstV1I GCAGC 4 cut(s) 46, 448, 451, 454
BstV2I GAAGAC 1 cut(s) 75
BsuI GTATCC 1 cut(s) 318
BsuRI GGCC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 443, 592
BtsIMutI CAGTG 2 cut(s) 38, 507
Csp6I GTAC 2 cut(s) 15, 753
CviAII CATG 1 cut(s) 730
CviJI RGCY 8 cut(s) 53, 59, 211, 226, 268, 280, 317, 385
CviKI_1 RGCY 8 cut(s) 53, 59, 211, 226, 268, 280, 317, 385
CviQI GTAC 2 cut(s) 15, 753
DdeI CTNAG 6 cut(s) 203, 221, 403, 581, 666, 681
DpnI GATC 2 cut(s) 428, 720
DpnII GATC 2 cut(s) 426, 718
DraI TTTAAA 1 cut(s) 153
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
Eam1104I CTCTTC 3 cut(s) 173, 185, 258
EarI CTCTTC 3 cut(s) 173, 185, 258
Ecl136II GAGCTC 1 cut(s) 268
Eco147I AGGCCT 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 62, 531
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 3 cut(s) 53, 328, 484
EcoRV GATATC 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 270
FaeI CATG 1 cut(s) 733
FaiI YATR 5 cut(s) 24, 176, 611, 731, 757
FalI AAGNNNNNCTT 1 cut(s) 33
FatI CATG 1 cut(s) 729
Fnu4HI GCNGC 4 cut(s) 60, 462, 465, 468
FokI GGATG 2 cut(s) 430, 599
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 4 cut(s) 60, 462, 465, 468
FspBI CTAG 1 cut(s) 129
GluI GCNGC 4 cut(s) 60, 462, 465, 468
HaeIII GGCC 1 cut(s) 226
Hin1I GRCGYC 1 cut(s) 699
Hin1II CATG 1 cut(s) 733
HinfI GANTC 1 cut(s) 677
HphI GGTGA 2 cut(s) 578, 706
Hpy188I TCNGA 6 cut(s) 110, 222, 239, 582, 646, 704
Hpy99I CGWCG 4 cut(s) 95, 641, 701, 704
HpyAV CCTTC 1 cut(s) 315
HpyCH4III ACNGT 2 cut(s) 104, 560
HpyCH4IV ACGT 4 cut(s) 90, 172, 639, 699
HpyCH4V TGCA 2 cut(s) 38, 62
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 458, 467
HpyF3I CTNAG 6 cut(s) 203, 221, 403, 581, 666, 681
HpySE526I ACGT 4 cut(s) 90, 172, 639, 699
Hsp92I GRCGYC 1 cut(s) 699
Hsp92II CATG 1 cut(s) 733
Kzo9I GATC 2 cut(s) 426, 718
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 2 cut(s) 50, 285
Lsp1109I GCAGC 4 cut(s) 46, 448, 451, 454
LweI GCATC 1 cut(s) 25
MaeI CTAG 1 cut(s) 129
MaeII ACGT 4 cut(s) 90, 172, 639, 699
MaeIII GTNAC 1 cut(s) 505
MalI GATC 2 cut(s) 428, 720
MboI GATC 2 cut(s) 426, 718
MboII GAAGA 7 cut(s) 55, 80, 190, 202, 266, 272, 275
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 5 cut(s) 142, 154, 364, 604, 670
MmeI TCCRAC 6 cut(s) 88, 496, 502, 508, 514, 682
MnlI CCTC 8 cut(s) 216, 233, 372, 415, 427, 457, 469, 576
MroXI GAANNNNTTC 1 cut(s) 605
MseI TTAA 4 cut(s) 152, 249, 374, 760
MspR9I CCNGG 3 cut(s) 55, 330, 486
MvaI CCWGG 3 cut(s) 55, 330, 486
MwoI GCNNNNNNNGC 3 cut(s) 59, 458, 467
NdeII GATC 2 cut(s) 426, 718
NlaIII CATG 1 cut(s) 733
NlaIV GGNNCC 2 cut(s) 52, 281
NmuCI GTSAC 1 cut(s) 505
NspI RCATGY 1 cut(s) 733
PceI AGGCCT 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 1 cut(s) 677
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 4 cut(s) 61, 463, 466, 469
Ppu21I YACGTR 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 3 cut(s) 53, 328, 484
PspGI CCWGG 3 cut(s) 53, 328, 484
PspN4I GGNNCC 2 cut(s) 52, 281
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 2 cut(s) 16, 754
RsaNI GTAC 2 cut(s) 15, 753
SacI GAGCTC 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 258
SaqAI TTAA 4 cut(s) 152, 249, 374, 760
SatI GCNGC 4 cut(s) 60, 462, 465, 468
Sau3AI GATC 2 cut(s) 426, 718
ScaI AGTACT 1 cut(s) 16
ScrFI CCNGG 3 cut(s) 55, 330, 486
SduI GDGCHC 1 cut(s) 270
SfaNI GCATC 1 cut(s) 25
Sse9I AATT 5 cut(s) 142, 154, 364, 604, 670
SseBI AGGCCT 1 cut(s) 226
SsiI CCGC 1 cut(s) 614
SspI AATATT 1 cut(s) 742
SspMI CTAG 1 cut(s) 129
SstI GAGCTC 1 cut(s) 270
StuI AGGCCT 1 cut(s) 226
StyD4I CCNGG 3 cut(s) 53, 328, 484
TaaI ACNGT 2 cut(s) 104, 560
TaiI ACGT 4 cut(s) 93, 175, 642, 702
TaqI TCGA 3 cut(s) 93, 636, 696
TasI AATT 5 cut(s) 142, 154, 364, 604, 670
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 1 cut(s) 677
Tru1I TTAA 4 cut(s) 152, 249, 374, 760
Tru9I TTAA 4 cut(s) 152, 249, 374, 760
TscAI CASTG 2 cut(s) 45, 514
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 4 cut(s) 59, 461, 464, 467
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 6 cut(s) 155, 191, 273, 360, 588, 598
TspRI CASTG 2 cut(s) 45, 514
Tth111I GACNNNGTC 1 cut(s) 89
XagI CCTNNNNNAGG 1 cut(s) 376
XapI RAATTY 2 cut(s) 154, 364
XceI RCATGY 1 cut(s) 733
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 129
ZraI GACGTC 1 cut(s) 700
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.