Rh5DG377500

Chloroplast import apparatus Tic20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010435
Physical Location & Seq
Forward (+)
57458675 .. 57460184
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG377500.1

Sequence Viewer

Length: 639 bp
ATGGCCATGTCCAACTTCCTCTCCCCCACAACCCCTCTTCGCCTCTCCCACAGACCCTTTATTCACTCCTCCCCATTCTCTCTAACCCACCCATCTCTTCTCACTCTCACCCACCTCTCCACCAACCCAGTCAAACCCAGAAGACTCCTGACCACCCAATCCAAGGGTGACGACTCAGTCGACGTCCCCGACCGCTTAATCGCCGCCGTCTGTTACTTCTACCCCTTCTTCGACGGCATACAATACGGTAAATATGTGATTACTCAGTTCACCGCCATTCAAGCTCTGATTAACCCCTTGGTGCCGGCTATACGGGTCTTCAAGAGCTTCCCCTTTAATGGGTTCTTGGTATTTTTAACCCTCTACTTTGGTGTTGTTAGGAACCAGAACTTTAGTAGGTATGTGAGGTTCAATACCATGCAAGTCATTGTGCTTGATGTGCTGCTCATTTTTCCTGATCTTCTGGAGAGGAGCTTCAATCCCAGAGATGGGTTGGGATTGGATGTGCTGATGAGCTTGGATAGTACCGTCTTCCTTTTCCTCTTGGTGTGTTTGATTTATGCGTCTTCTTCTTGCTTGTTGGGTCAGGTCCCCAGATTGCCCATTGTAGCTGAAGCTGCTGATAGGCAGGTTCCTTGA

Protein Analysis

212

Amino Acids

23.89

Weight (kDa)

9.12

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 65 - 207 9.4e-53 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AatII GACGTC 1 cut(s) 186
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 301
AccI GTMKAC 1 cut(s) 180
AciI CCGC 3 cut(s) 193, 204, 273
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 633
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 1 cut(s) 526
AfiI CCNNNNNNNGG 5 cut(s) 163, 338, 339, 488, 489
AgsI TTSAA 4 cut(s) 281, 322, 412, 478
AluBI AGCT 6 cut(s) 284, 327, 474, 516, 611, 617
AluI AGCT 6 cut(s) 284, 327, 474, 516, 611, 617
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 442, 617
AspS9I GGNCC 1 cut(s) 589
AsuHPI GGTGA 3 cut(s) 100, 179, 262
AvaII GGWCC 1 cut(s) 589
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 301
BbsI GAAGAC 4 cut(s) 148, 310, 523, 558
BbvI GCAGC 2 cut(s) 429, 604
BccI CCATC 2 cut(s) 100, 482
BceAI ACGGC 2 cut(s) 191, 250
BfuAI ACCTGC 1 cut(s) 619
BisI GCNGC 3 cut(s) 204, 443, 618
BlsI GCNGC 3 cut(s) 205, 444, 619
Bme18I GGWCC 1 cut(s) 589
BmgT120I GGNCC 1 cut(s) 589
BmiI GGNNCC 4 cut(s) 303, 383, 591, 633
BmrI ACTGGG 1 cut(s) 122
BmuI ACTGGG 1 cut(s) 122
BpiI GAAGAC 4 cut(s) 148, 310, 523, 558
BpmI CTGGAG 1 cut(s) 485
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 162, 297
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 338, 339, 488, 489
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 128
BseDI CCNNGG 2 cut(s) 162, 297
BseGI GGATG 1 cut(s) 508
BseLI CCNNNNNNNGG 5 cut(s) 163, 338, 339, 488, 489
BseMII CTCAG 2 cut(s) 189, 278
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 2 cut(s) 58, 484
BseXI GCAGC 2 cut(s) 429, 604
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 193
BsiSI CCGG 1 cut(s) 305
BslFI GGGAC 2 cut(s) 170, 575
BslI CCNNNNNNNGG 5 cut(s) 163, 338, 339, 488, 489
BsmFI GGGAC 2 cut(s) 170, 575
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 457
BspACI CCGC 3 cut(s) 193, 204, 273
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 188, 277
BspLI GGNNCC 4 cut(s) 303, 383, 591, 633
BspMI ACCTGC 1 cut(s) 619
BspT107I GGYRCC 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 162, 297
BssMI GATC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 162, 297
Bst4CI ACNGT 2 cut(s) 248, 529
Bst6I CTCTTC 2 cut(s) 42, 102
BstACI GRCGYC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 2 cut(s) 175, 264
BstF5I GGATG 1 cut(s) 508
BstKTI GATC 1 cut(s) 460
BstMBI GATC 1 cut(s) 457
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 3 cut(s) 281, 439, 617
BstV1I GCAGC 2 cut(s) 429, 604
BstV2I GAAGAC 4 cut(s) 148, 310, 523, 558
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 508
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 1 cut(s) 306
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 589
CseI GACGC 1 cut(s) 552
Csp6I GTAC 1 cut(s) 525
CviAII CATG 2 cut(s) 7, 418
CviJI RGCY 8 cut(s) 5, 284, 308, 327, 474, 516, 611, 617
CviKI_1 RGCY 8 cut(s) 5, 284, 308, 327, 474, 516, 611, 617
CviQI GTAC 1 cut(s) 525
DdeI CTNAG 2 cut(s) 175, 264
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 42, 102
EarI CTCTTC 2 cut(s) 42, 102
Eco130I CCWWGG 2 cut(s) 162, 297
Eco47I GGWCC 1 cut(s) 589
Eco57I CTGAAG 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 589
EcoT14I CCWWGG 2 cut(s) 162, 297
ErhI CCWWGG 2 cut(s) 162, 297
FaeI CATG 2 cut(s) 10, 421
FaiI YATR 7 cut(s) 8, 239, 255, 311, 402, 419, 561
FaqI GGGAC 2 cut(s) 170, 575
FatI CATG 2 cut(s) 6, 417
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 3 cut(s) 204, 443, 618
FokI GGATG 1 cut(s) 515
Fsp4HI GCNGC 3 cut(s) 204, 443, 618
GluI GCNGC 3 cut(s) 204, 443, 618
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 305
HgaI GACGC 1 cut(s) 552
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 2 cut(s) 10, 421
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 2 cut(s) 144, 173
HpaII CCGG 1 cut(s) 305
HphI GGTGA 3 cut(s) 100, 179, 262
Hpy166II GTNNAC 2 cut(s) 181, 270
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 4 cut(s) 148, 322, 455, 464
Hpy8I GTNNAC 2 cut(s) 181, 270
Hpy99I CGWCG 2 cut(s) 185, 236
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 2 cut(s) 248, 529
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 1 cut(s) 421
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 439, 617
HpyF3I CTNAG 2 cut(s) 175, 264
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 2 cut(s) 10, 421
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 2 cut(s) 429, 604
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 167, 212
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 9 cut(s) 29, 89, 153, 220, 310, 452, 523, 558, 561
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 138, 167
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 9 cut(s) 29, 45, 53, 79, 125, 371, 399, 462, 551
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 304
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 197, 291, 336, 356
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 305
MwoI GCNNNNNNNGC 3 cut(s) 281, 439, 617
NaeI GCCGGC 1 cut(s) 306
NdeII GATC 1 cut(s) 457
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 2 cut(s) 10, 421
NlaIV GGNNCC 4 cut(s) 303, 383, 591, 633
NmuCI GTSAC 1 cut(s) 167
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 186
PdiI GCCGGC 1 cut(s) 306
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 3 cut(s) 205, 444, 619
PleI GAGTC 2 cut(s) 138, 167
PpsI GAGTC 2 cut(s) 138, 167
PpuMI RGGWCCY 1 cut(s) 589
Psp5II RGGWCCY 1 cut(s) 589
PspN4I GGNNCC 4 cut(s) 303, 383, 591, 633
PspPI GGNCC 1 cut(s) 589
PspPPI RGGWCCY 1 cut(s) 589
PsrI GAACNNNNNNTAC 2 cut(s) 392, 424
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 1 cut(s) 526
RsaNI GTAC 1 cut(s) 525
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 4 cut(s) 197, 291, 336, 356
SatI GCNGC 3 cut(s) 204, 443, 618
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 1 cut(s) 589
SchI GAGTC 2 cut(s) 138, 167
SinI GGWCC 1 cut(s) 589
SsiI CCGC 3 cut(s) 193, 204, 273
StyI CCWWGG 2 cut(s) 162, 297
TaaI ACNGT 2 cut(s) 248, 529
TaiI ACGT 1 cut(s) 186
TaqI TCGA 2 cut(s) 180, 231
TauI GCSGC 1 cut(s) 206
Tru1I TTAA 4 cut(s) 197, 291, 336, 356
Tru9I TTAA 4 cut(s) 197, 291, 336, 356
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 442, 617
Tsp45I GTSAC 1 cut(s) 167
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 1 cut(s) 490
XmiI GTMKAC 1 cut(s) 180
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.