MD11G1198300.v1.1

Chloroplast import apparatus Tic20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010435
Physical Location & Seq
Forward (+)
28591422 .. 28591924
503 bp
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UTR
Exon/CDS
Intron
MD11G1198300.v1.1.491

Sequence Viewer

Length: 432 bp
ATGTCCAGCCTTCTCTCTCCCATCACCCCTCTTACAGTCACGCTCTCTCACTCCTCCAAACCCCTCCTCCACCTCCACTCCCCATTCCTCTTCTCTCTAAACCACCACCACTCTTCTCTCACTAAATCCACCACCAAACTCAAACCTAGAAGACCCCTCACCACCACGCAATCCAAGGGCACCGACGACTCCGTCGACGCCCCCAACCGCCTCATCTCCGTCGTCTGTTACTTCTACCCCTTCTTCGACGGATCCAGTACGGAAACTCGAATTTTAGCAGATATCTTTAATCCGAAAGACTGGTTGGGATTGGATAGTACGGTGTTTCTCTTCCTTTTGGTGTGCTTGGTTTATGGGTCTGCTTCTTGCGTTTTGGGTCAGGTTCCCAGATTGCCCATTGTACCTGAAGCTGCTGGGAGGCAAGTTCCTTGA

Protein Analysis

144

Amino Acids

15.71

Weight (kDa)

8.91

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 101 - 138 1.5e-07 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 195
AciI CCGC 1 cut(s) 208
AclWI GGATC 2 cut(s) 246, 259
AcsI RAATTY 1 cut(s) 270
AcuI CTGAAG 1 cut(s) 426
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 3 cut(s) 259, 319, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
AlwI GGATC 2 cut(s) 246, 259
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 1 cut(s) 270
AsuHPI GGTGA 2 cut(s) 16, 151
BaeGI GKGCMC 1 cut(s) 182
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 157
BbvI GCAGC 1 cut(s) 397
BccI CCATC 1 cut(s) 29
BfaI CTAG 1 cut(s) 147
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
BmiI GGNNCC 3 cut(s) 181, 253, 384
BpiI GAAGAC 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 174
BsaXI ACNNNNNCTCC 4 cut(s) 51, 62, 81, 92
Bse1I ACTGG 2 cut(s) 255, 305
BseDI CCNNGG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 255, 305
BseRI GAGGAG 2 cut(s) 43, 56
BseSI GKGCMC 1 cut(s) 182
BseXI GCAGC 1 cut(s) 397
BseYI CCCAGC 1 cut(s) 413
BshNI GGYRCC 1 cut(s) 179
Bsp1286I GDGCHC 1 cut(s) 182
Bsp143I GATC 1 cut(s) 251
BspACI CCGC 1 cut(s) 208
BspLI GGNNCC 3 cut(s) 181, 253, 384
BspPI GGATC 2 cut(s) 246, 259
BspT107I GGYRCC 1 cut(s) 179
BsrI ACTGG 2 cut(s) 255, 305
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 1 cut(s) 251
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 37, 322
Bst6I CTCTTC 3 cut(s) 95, 118, 335
BstACI GRCGYC 1 cut(s) 198
BstKTI GATC 1 cut(s) 254
BstMBI GATC 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 1 cut(s) 157
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
CseI GACGC 1 cut(s) 206
Csp6I GTAC 3 cut(s) 258, 318, 401
CviJI RGCY 2 cut(s) 9, 410
CviKI_1 RGCY 2 cut(s) 9, 410
CviQI GTAC 3 cut(s) 258, 318, 401
DpnI GATC 1 cut(s) 253
DpnII GATC 1 cut(s) 251
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Eam1104I CTCTTC 3 cut(s) 95, 118, 335
EarI CTCTTC 3 cut(s) 95, 118, 335
Eco130I CCWWGG 1 cut(s) 174
Eco32I GATATC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 426
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
FaiI YATR 1 cut(s) 354
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 411
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 1 cut(s) 147
GluI GCNGC 1 cut(s) 411
GsaI CCCAGC 1 cut(s) 417
HgaI GACGC 1 cut(s) 206
Hin1I GRCGYC 1 cut(s) 198
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 1 cut(s) 188
HphI GGTGA 2 cut(s) 16, 151
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 1 cut(s) 294
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 5 cut(s) 188, 197, 200, 224, 251
HpyAV CCTTC 2 cut(s) 20, 250
HpyCH4III ACNGT 2 cut(s) 37, 322
Hsp92I GRCGYC 1 cut(s) 198
Kzo9I GATC 1 cut(s) 251
LpnPI CCDG 7 cut(s) 19, 268, 286, 365, 399, 400, 417
Lsp1109I GCAGC 1 cut(s) 397
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 2 cut(s) 37, 227
MalI GATC 1 cut(s) 253
MboI GATC 1 cut(s) 251
MboII GAAGA 5 cut(s) 82, 105, 162, 235, 322
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 1 cut(s) 182
MluCI AATT 1 cut(s) 270
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 9 cut(s) 39, 64, 74, 77, 83, 98, 167, 221, 411
MseI TTAA 1 cut(s) 288
NdeII GATC 1 cut(s) 251
NlaIV GGNNCC 3 cut(s) 181, 253, 384
NmuCI GTSAC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PflFI GACNNNGTC 1 cut(s) 191
PkrI GCNGC 1 cut(s) 412
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
PspFI CCCAGC 1 cut(s) 413
PspN4I GGNNCC 3 cut(s) 181, 253, 384
PsuI RGATCY 1 cut(s) 251
PsyI GACNNNGTC 1 cut(s) 191
RsaI GTAC 3 cut(s) 259, 319, 402
RsaNI GTAC 3 cut(s) 258, 318, 401
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 1 cut(s) 251
SchI GAGTC 1 cut(s) 182
SduI GDGCHC 1 cut(s) 182
SetI ASST 5 cut(s) 75, 148, 384, 406, 412
SgrDI CGTCGACG 1 cut(s) 194
Sse9I AATT 1 cut(s) 270
SsiI CCGC 1 cut(s) 208
SspMI CTAG 1 cut(s) 147
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 2 cut(s) 37, 322
TaqI TCGA 3 cut(s) 195, 246, 268
TasI AATT 1 cut(s) 270
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 37
TspGWI ACGGA 4 cut(s) 181, 208, 264, 275
Tth111I GACNNNGTC 1 cut(s) 191
XapI RAATTY 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.