Rh5BG363200

Chloroplast import apparatus Tic20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010435
Physical Location & Seq
Forward (+)
56886727 .. 56888237
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG363200.1

Sequence Viewer

Length: 780 bp
ATGCCCATAAACGAGGTCGTTTTAATTTTCTTCCATCTTGAATGCAAAGATATTCGTTTGGGCCAATCTGAGTCGCTGTCTTGCTCCAGATTAACTCCTCCCCTTCACCCAAAAAACCCGGACTTCCATTTCTCTGTAGAAATGGCCATGTCCAACTTCCTCTCCCCCACAACCCCTCTTCGCCTCTCCCACAGACCCTTTATTCACTCCTCCCCATTCTCTCTAACCCACCATTCTCTTCTCACTCTCACCCACCTCTCCACCAACCCAGTCAAACCCAGAAGACTCCTGACCACCCAATCCAAGGGTGACGACTCAGTCGACGTCCCCGACCGCTTAATCGCCGCCGTCTGTTACTTCTACCCCTTCTTCGACGGCATACAATACGGTAAATATGTGATCACTCAGTTCACCGCCATTCAAGCTCTGATTAACCCCTTGGTGCCGGCTATACGGGTCTTCAAGAGCTTCCCCTTTAATGGGTTCTTGGTATTTTTAACCCTCTACTTTGGTGTTGTTAGGAACCAGAACTTTAGTAGGTATGTGAGGTTCAATACCATGCAAGTCATTGTGCTTGATGTGCTGCTTATTTTTCCTGATCTTCTGGAGAGGAGCTTCAATCCCAGAGATGGGTTGGGATTGGATGTGCTGATGAGCTTGGATAGTACCGTCTTCCTTTTCCTCTTGGTGTGTTTGATTTATGCGTCTTCTTCTTGCTTGTTGGGTCAGGTCCCCAGATTGCCCATTGTAGCTGAAGCTGCTGATAGGCAGGTTCCTTGA

Protein Analysis

259

Amino Acids

29.34

Weight (kDa)

8.3

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 112 - 254 1.7e-52 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 317
AatII GACGTC 1 cut(s) 327
Acc36I ACCTGC 1 cut(s) 760
AccB1I GGYRCC 1 cut(s) 442
AccI GTMKAC 1 cut(s) 321
AciI CCGC 3 cut(s) 334, 345, 414
AcoI YGGCCR 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 774
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 1 cut(s) 667
AfiI CCNNNNNNNGG 5 cut(s) 304, 479, 480, 629, 630
AgsI TTSAA 5 cut(s) 41, 422, 463, 553, 619
AluBI AGCT 6 cut(s) 425, 468, 615, 657, 752, 758
AluI AGCT 6 cut(s) 425, 468, 615, 657, 752, 758
AoxI GGCC 2 cut(s) 61, 144
ApeKI GCWGC 2 cut(s) 583, 758
AspS9I GGNCC 2 cut(s) 61, 730
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 4 cut(s) 98, 241, 320, 403
AvaII GGWCC 1 cut(s) 730
BalI TGGCCA 1 cut(s) 146
BanI GGYRCC 1 cut(s) 442
BbsI GAAGAC 4 cut(s) 289, 451, 664, 699
BbvI GCAGC 2 cut(s) 570, 745
BccI CCATC 2 cut(s) 42, 623
BceAI ACGGC 2 cut(s) 332, 391
BclI TGATCA 1 cut(s) 399
BcnI CCSGG 1 cut(s) 119
BfmI CTRYAG 1 cut(s) 135
BfuAI ACCTGC 1 cut(s) 760
BisI GCNGC 3 cut(s) 345, 584, 759
BlsI GCNGC 3 cut(s) 346, 585, 760
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 1 cut(s) 730
BmgT120I GGNCC 2 cut(s) 61, 730
BmiI GGNNCC 4 cut(s) 444, 524, 732, 774
BmrFI CCNGG 1 cut(s) 119
BmrI ACTGGG 1 cut(s) 263
BmuI ACTGGG 1 cut(s) 263
BpiI GAAGAC 4 cut(s) 289, 451, 664, 699
BpmI CTGGAG 2 cut(s) 70, 626
BpuMI CCSGG 1 cut(s) 119
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 303, 438
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 5 cut(s) 304, 479, 480, 629, 630
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 303, 438
BseGI GGATG 1 cut(s) 649
BseLI CCNNNNNNNGG 5 cut(s) 304, 479, 480, 629, 630
BseMII CTCAG 3 cut(s) 60, 330, 419
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 3 cut(s) 87, 199, 625
BseXI GCAGC 2 cut(s) 570, 745
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 2 cut(s) 63, 146
BshNI GGYRCC 1 cut(s) 442
BsiEI CGRYCG 1 cut(s) 334
BsiSI CCGG 2 cut(s) 119, 446
BslFI GGGAC 2 cut(s) 311, 716
BslI CCNNNNNNNGG 5 cut(s) 304, 479, 480, 629, 630
BsmFI GGGAC 2 cut(s) 311, 716
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 2 cut(s) 63, 146
Bsp143I GATC 2 cut(s) 399, 598
BspACI CCGC 3 cut(s) 334, 345, 414
BspANI GGCC 2 cut(s) 63, 146
BspCNI CTCAG 3 cut(s) 61, 329, 418
BspLI GGNNCC 4 cut(s) 444, 524, 732, 774
BspMI ACCTGC 1 cut(s) 760
BspT107I GGYRCC 1 cut(s) 442
BsrFI RCCGGY 1 cut(s) 445
BsrI ACTGG 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 303, 438
BssMI GATC 2 cut(s) 399, 598
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 303, 438
Bst4CI ACNGT 2 cut(s) 389, 670
Bst6I CTCTTC 2 cut(s) 183, 243
BstACI GRCGYC 1 cut(s) 324
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 69, 316, 405
BstF5I GGATG 1 cut(s) 649
BstKTI GATC 2 cut(s) 402, 601
BstMBI GATC 2 cut(s) 399, 598
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 3 cut(s) 422, 580, 758
BstSCI CCNGG 1 cut(s) 117
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 570, 745
BstV2I GAAGAC 4 cut(s) 289, 451, 664, 699
BsuRI GGCC 2 cut(s) 63, 146
BtsCI GGATG 1 cut(s) 649
BveI ACCTGC 1 cut(s) 760
Cac8I GCNNGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 2 cut(s) 61, 730
CseI GACGC 1 cut(s) 693
Csp6I GTAC 1 cut(s) 666
CviAII CATG 2 cut(s) 148, 559
CviJI RGCY 9 cut(s) 63, 146, 425, 449, 468, 615, 657, 752, 758
CviKI_1 RGCY 9 cut(s) 63, 146, 425, 449, 468, 615, 657, 752, 758
CviQI GTAC 1 cut(s) 666
DdeI CTNAG 3 cut(s) 69, 316, 405
DpnI GATC 2 cut(s) 401, 600
DpnII GATC 2 cut(s) 399, 598
DrdI GACNNNNNNGTC 1 cut(s) 317
DseDI GACNNNNNNGTC 1 cut(s) 317
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 2 cut(s) 183, 243
EarI CTCTTC 2 cut(s) 183, 243
Eco130I CCWWGG 2 cut(s) 303, 438
Eco47I GGWCC 1 cut(s) 730
Eco57I CTGAAG 1 cut(s) 774
EcoO109I RGGNCCY 1 cut(s) 730
EcoT14I CCWWGG 2 cut(s) 303, 438
ErhI CCWWGG 2 cut(s) 303, 438
FaeI CATG 2 cut(s) 151, 562
FaiI YATR 8 cut(s) 8, 149, 380, 396, 452, 543, 560, 702
FaqI GGGAC 2 cut(s) 311, 716
FatI CATG 2 cut(s) 147, 558
FbaI TGATCA 1 cut(s) 399
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 3 cut(s) 345, 584, 759
FokI GGATG 1 cut(s) 656
Fsp4HI GCNGC 3 cut(s) 345, 584, 759
GluI GCNGC 3 cut(s) 345, 584, 759
GsuI CTGGAG 2 cut(s) 70, 626
HaeIII GGCC 2 cut(s) 63, 146
HapII CCGG 2 cut(s) 119, 446
HgaI GACGC 1 cut(s) 693
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 2 cut(s) 151, 562
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 3 cut(s) 71, 285, 314
HpaII CCGG 2 cut(s) 119, 446
HphI GGTGA 4 cut(s) 98, 241, 320, 403
Hpy166II GTNNAC 2 cut(s) 322, 411
Hpy188I TCNGA 2 cut(s) 70, 429
Hpy188III TCNNGA 6 cut(s) 38, 87, 289, 463, 596, 605
Hpy8I GTNNAC 2 cut(s) 322, 411
Hpy99I CGWCG 2 cut(s) 326, 377
HpyAV CCTTC 2 cut(s) 113, 376
HpyCH4III ACNGT 2 cut(s) 389, 670
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 2 cut(s) 45, 562
HpyF10VI GCNNNNNNNGC 3 cut(s) 422, 580, 758
HpyF3I CTNAG 3 cut(s) 69, 316, 405
HpySE526I ACGT 1 cut(s) 324
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 2 cut(s) 151, 562
KroI GCCGGC 1 cut(s) 445
KroNI GCCGGC 1 cut(s) 447
Ksp22I TGATCA 1 cut(s) 399
Kzo9I GATC 2 cut(s) 399, 598
LmnI GCTCC 2 cut(s) 89, 612
Lsp1109I GCAGC 2 cut(s) 570, 745
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 308, 353
MalI GATC 2 cut(s) 401, 600
MboI GATC 2 cut(s) 399, 598
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 1 cut(s) 24
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 3 cut(s) 80, 279, 308
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 445
MscI TGGCCA 1 cut(s) 146
MseI TTAA 6 cut(s) 23, 92, 338, 432, 477, 497
Msp20I TGGCCA 1 cut(s) 146
MspI CCGG 2 cut(s) 119, 446
MspR9I CCNGG 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 47
MwoI GCNNNNNNNGC 3 cut(s) 422, 580, 758
NaeI GCCGGC 1 cut(s) 447
NciI CCSGG 1 cut(s) 119
NdeII GATC 2 cut(s) 399, 598
NgoMIV GCCGGC 1 cut(s) 445
NlaIII CATG 2 cut(s) 151, 562
NlaIV GGNNCC 4 cut(s) 444, 524, 732, 774
NmuCI GTSAC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 327
PctI GAATGC 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 447
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 3 cut(s) 346, 585, 760
PleI GAGTC 3 cut(s) 79, 279, 308
PpsI GAGTC 3 cut(s) 79, 279, 308
PpuMI RGGWCCY 1 cut(s) 730
Psp5II RGGWCCY 1 cut(s) 730
PspN4I GGNNCC 4 cut(s) 444, 524, 732, 774
PspPI GGNCC 2 cut(s) 61, 730
PspPPI RGGWCCY 1 cut(s) 730
PsrI GAACNNNNNNTAC 2 cut(s) 533, 565
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 1 cut(s) 667
RsaNI GTAC 1 cut(s) 666
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 6 cut(s) 23, 92, 338, 432, 477, 497
SatI GCNGC 3 cut(s) 345, 584, 759
Sau3AI GATC 2 cut(s) 399, 598
Sau96I GGNCC 2 cut(s) 61, 730
SchI GAGTC 3 cut(s) 80, 279, 308
ScrFI CCNGG 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 135
SinI GGWCC 1 cut(s) 730
Sse9I AATT 1 cut(s) 24
SsiI CCGC 3 cut(s) 334, 345, 414
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 303, 438
TaaI ACNGT 2 cut(s) 389, 670
TaiI ACGT 1 cut(s) 327
TaqI TCGA 2 cut(s) 321, 372
TasI AATT 1 cut(s) 24
TauI GCSGC 1 cut(s) 347
Tru1I TTAA 6 cut(s) 23, 92, 338, 432, 477, 497
Tru9I TTAA 6 cut(s) 23, 92, 338, 432, 477, 497
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 2 cut(s) 583, 758
Tsp45I GTSAC 1 cut(s) 308
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 321
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.