Rh5AG350900

Chloroplast import apparatus Tic20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010435
Physical Location & Seq
Forward (+)
58116068 .. 58117492
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG350900.1

Sequence Viewer

Length: 768 bp
ATGCCCATAAACGAGGTCGTTTTAATTTTCTTCCATCTTGAATGCAAAGATATTCGTTTGGGCCAATCTGAGTCGCTGTCTTGCTCCAGATTAACTCCTCCCCTTCACCCAAAAAACCCGGACTTCCATTTCTACAGAGAAATGGCCATGTCCAACTTCCTCTCCCCCACAACCCCTCTTTGCCTCTCCCACAGACCCTTTATTCACTCCTCCCCATTCTCTCTAACCCACCAGTCTCTTCTCACTCTCACCAACCCAGTCAAACCCAGAAGACTCCTGACCACCCAATCCAAGGGTGACGACTCAGTCGACGTCCCCGACCGCTTAATCGCCGCCGTCTGTTACTTCTACCCCTTCTTCGACGGCATACAATACGGTAAATATGTGATCACTCAGTTCACCGCCATTCAAGCTCTGATTAACCCCTTGGTGCCGGCTATACGGGTCTTCAAGAGCTTCCCCTTTAATGGGTTCTTGGTATTTTTAACCCTCTACTTTGGTGTTGTTAGGAACCAGAACTTTAGTAGGTATGTGAGGTTCAATACCATGCAAGTCATTGTGCTTGATGTGCTGCTCATTTTTCCTGATCTTCTGGAGAGGAGCTTCAATCCCAGAGATGGGTTGGGATTGGATGTGCTGATGAGCTTGGATAGTACCGTGTTCCTTTTCCTCTTGGTGTGTTTGATTTATGCGTCTTCTTCTTGCTTGTTGGGTCAGGTCCCCAGATTGCCCATTGTAGCTGAAGCTGCTGATAGGCAGGTTCCTTGA

Protein Analysis

255

Amino Acids

28.97

Weight (kDa)

8.22

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 108 - 250 1.7e-52 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AatII GACGTC 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 748
AccB1I GGYRCC 1 cut(s) 430
AccI GTMKAC 1 cut(s) 309
AciI CCGC 3 cut(s) 322, 333, 402
AcoI YGGCCR 1 cut(s) 144
AcuI CTGAAG 1 cut(s) 762
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 1 cut(s) 655
AfiI CCNNNNNNNGG 5 cut(s) 292, 467, 468, 617, 618
AgsI TTSAA 5 cut(s) 41, 410, 451, 541, 607
AluBI AGCT 6 cut(s) 413, 456, 603, 645, 740, 746
AluI AGCT 6 cut(s) 413, 456, 603, 645, 740, 746
Alw26I GTCTC 1 cut(s) 240
AoxI GGCC 2 cut(s) 61, 144
ApeKI GCWGC 2 cut(s) 571, 746
AspS9I GGNCC 2 cut(s) 61, 718
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 4 cut(s) 98, 241, 308, 391
AvaII GGWCC 1 cut(s) 718
BalI TGGCCA 1 cut(s) 146
BanI GGYRCC 1 cut(s) 430
BbsI GAAGAC 3 cut(s) 277, 439, 687
BbvI GCAGC 2 cut(s) 558, 733
BccI CCATC 2 cut(s) 42, 611
BceAI ACGGC 2 cut(s) 320, 379
BclI TGATCA 1 cut(s) 387
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 240
BfmI CTRYAG 1 cut(s) 133
BfuAI ACCTGC 1 cut(s) 748
BisI GCNGC 3 cut(s) 333, 572, 747
BlsI GCNGC 3 cut(s) 334, 573, 748
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 1 cut(s) 718
BmgT120I GGNCC 2 cut(s) 61, 718
BmiI GGNNCC 4 cut(s) 432, 512, 720, 762
BmrFI CCNGG 1 cut(s) 119
BmrI ACTGGG 1 cut(s) 251
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 3 cut(s) 277, 439, 687
BpmI CTGGAG 2 cut(s) 70, 614
BpuMI CCSGG 1 cut(s) 119
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 291, 426
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 5 cut(s) 292, 467, 468, 617, 618
Bse118I RCCGGY 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 232, 257
BseDI CCNNGG 2 cut(s) 291, 426
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 5 cut(s) 292, 467, 468, 617, 618
BseMII CTCAG 3 cut(s) 60, 318, 407
BseNI ACTGG 2 cut(s) 232, 257
BseRI GAGGAG 3 cut(s) 87, 199, 613
BseXI GCAGC 2 cut(s) 558, 733
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 63, 146
BshNI GGYRCC 1 cut(s) 430
BsiEI CGRYCG 1 cut(s) 322
BsiSI CCGG 2 cut(s) 119, 434
BslFI GGGAC 2 cut(s) 299, 704
BslI CCNNNNNNNGG 5 cut(s) 292, 467, 468, 617, 618
BsmAI GTCTC 1 cut(s) 240
BsmFI GGGAC 2 cut(s) 299, 704
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 2 cut(s) 63, 146
Bsp143I GATC 2 cut(s) 387, 586
BspACI CCGC 3 cut(s) 322, 333, 402
BspANI GGCC 2 cut(s) 63, 146
BspCNI CTCAG 3 cut(s) 61, 317, 406
BspLI GGNNCC 4 cut(s) 432, 512, 720, 762
BspMI ACCTGC 1 cut(s) 748
BspT107I GGYRCC 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 433
BsrI ACTGG 2 cut(s) 232, 257
BssAI RCCGGY 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 291, 426
BssMI GATC 2 cut(s) 387, 586
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 2 cut(s) 291, 426
Bst4CI ACNGT 2 cut(s) 377, 658
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 1 cut(s) 312
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 3 cut(s) 69, 304, 393
BstF5I GGATG 1 cut(s) 637
BstKTI GATC 2 cut(s) 390, 589
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 2 cut(s) 387, 586
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 3 cut(s) 410, 568, 746
BstSCI CCNGG 1 cut(s) 117
BstSFI CTRYAG 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 558, 733
BstV2I GAAGAC 3 cut(s) 277, 439, 687
BsuRI GGCC 2 cut(s) 63, 146
BtsCI GGATG 1 cut(s) 637
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 433
Cfr13I GGNCC 2 cut(s) 61, 718
CseI GACGC 1 cut(s) 681
Csp6I GTAC 1 cut(s) 654
CviAII CATG 2 cut(s) 148, 547
CviJI RGCY 9 cut(s) 63, 146, 413, 437, 456, 603, 645, 740, 746
CviKI_1 RGCY 9 cut(s) 63, 146, 413, 437, 456, 603, 645, 740, 746
CviQI GTAC 1 cut(s) 654
DdeI CTNAG 3 cut(s) 69, 304, 393
DpnI GATC 2 cut(s) 389, 588
DpnII GATC 2 cut(s) 387, 586
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 291, 426
Eco47I GGWCC 1 cut(s) 718
Eco57I CTGAAG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 291, 426
ErhI CCWWGG 2 cut(s) 291, 426
FaeI CATG 2 cut(s) 151, 550
FaiI YATR 8 cut(s) 8, 149, 368, 384, 440, 531, 548, 690
FaqI GGGAC 2 cut(s) 299, 704
FatI CATG 2 cut(s) 147, 546
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 333, 572, 747
FokI GGATG 1 cut(s) 644
Fsp4HI GCNGC 3 cut(s) 333, 572, 747
GluI GCNGC 3 cut(s) 333, 572, 747
GsuI CTGGAG 2 cut(s) 70, 614
HaeIII GGCC 2 cut(s) 63, 146
HapII CCGG 2 cut(s) 119, 434
HgaI GACGC 1 cut(s) 681
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 2 cut(s) 151, 550
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 3 cut(s) 71, 273, 302
HpaII CCGG 2 cut(s) 119, 434
HphI GGTGA 4 cut(s) 98, 241, 308, 391
Hpy166II GTNNAC 2 cut(s) 310, 399
Hpy188I TCNGA 2 cut(s) 70, 417
Hpy188III TCNNGA 6 cut(s) 38, 87, 277, 451, 584, 593
Hpy8I GTNNAC 2 cut(s) 310, 399
Hpy99I CGWCG 2 cut(s) 314, 365
HpyAV CCTTC 2 cut(s) 113, 364
HpyCH4III ACNGT 2 cut(s) 377, 658
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 45, 550
HpyF10VI GCNNNNNNNGC 3 cut(s) 410, 568, 746
HpyF3I CTNAG 3 cut(s) 69, 304, 393
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 2 cut(s) 151, 550
KroI GCCGGC 1 cut(s) 433
KroNI GCCGGC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 2 cut(s) 387, 586
LmnI GCTCC 2 cut(s) 89, 600
Lsp1109I GCAGC 2 cut(s) 558, 733
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 296, 341
MalI GATC 2 cut(s) 389, 588
MboI GATC 2 cut(s) 387, 586
MboII GAAGA 8 cut(s) 22, 230, 282, 349, 439, 581, 687, 690
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 1 cut(s) 24
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 3 cut(s) 80, 267, 296
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 1 cut(s) 146
MroNI GCCGGC 1 cut(s) 433
MscI TGGCCA 1 cut(s) 146
MseI TTAA 6 cut(s) 23, 92, 326, 420, 465, 485
Msp20I TGGCCA 1 cut(s) 146
MspI CCGG 2 cut(s) 119, 434
MspR9I CCNGG 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 47
MwoI GCNNNNNNNGC 3 cut(s) 410, 568, 746
NaeI GCCGGC 1 cut(s) 435
NciI CCSGG 1 cut(s) 119
NdeII GATC 2 cut(s) 387, 586
NgoMIV GCCGGC 1 cut(s) 433
NlaIII CATG 2 cut(s) 151, 550
NlaIV GGNNCC 4 cut(s) 432, 512, 720, 762
NmuCI GTSAC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 2 cut(s) 306, 315
PctI GAATGC 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 435
PflFI GACNNNGTC 1 cut(s) 305
PkrI GCNGC 3 cut(s) 334, 573, 748
PleI GAGTC 3 cut(s) 79, 267, 296
PpsI GAGTC 3 cut(s) 79, 267, 296
PpuMI RGGWCCY 1 cut(s) 718
Psp5II RGGWCCY 1 cut(s) 718
PspN4I GGNNCC 4 cut(s) 432, 512, 720, 762
PspPI GGNCC 2 cut(s) 61, 718
PspPPI RGGWCCY 1 cut(s) 718
PsrI GAACNNNNNNTAC 2 cut(s) 521, 553
PsyI GACNNNGTC 1 cut(s) 305
RsaI GTAC 1 cut(s) 655
RsaNI GTAC 1 cut(s) 654
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 6 cut(s) 23, 92, 326, 420, 465, 485
SatI GCNGC 3 cut(s) 333, 572, 747
Sau3AI GATC 2 cut(s) 387, 586
Sau96I GGNCC 2 cut(s) 61, 718
SchI GAGTC 3 cut(s) 80, 267, 296
ScrFI CCNGG 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 133
SinI GGWCC 1 cut(s) 718
Sse9I AATT 1 cut(s) 24
SsiI CCGC 3 cut(s) 322, 333, 402
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 291, 426
TaaI ACNGT 2 cut(s) 377, 658
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 309, 360
TasI AATT 1 cut(s) 24
TauI GCSGC 1 cut(s) 335
Tru1I TTAA 6 cut(s) 23, 92, 326, 420, 465, 485
Tru9I TTAA 6 cut(s) 23, 92, 326, 420, 465, 485
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 571, 746
Tsp45I GTSAC 1 cut(s) 296
Tth111I GACNNNGTC 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XmiI GTMKAC 1 cut(s) 309
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.