pycom03g13510

Chloroplast import apparatus Tic20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010435
Physical Location & Seq
Forward (+)
14572383 .. 14573126
744 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13510.1

Sequence Viewer

Length: 744 bp
ATGACCCAATCTCTCACGCATCTTTCTCTTCTCCTCTTTCCAGTCCACCCAAAAACCCAAACCTTTCATTTCATTTCCTCCAAAGAGATGGCTATGTCCGGCCTTCTCTCTCCCACCACCCCTCTCACCGTCACCCTCTCTCCCTCCAAACCCCTCCTCCGCCTCCACTCCCCCTTCCTCTTCTCTCTAAACCACCACCACTCCATCCTCACTAAACACACCAACAAAATCAAACCCAGAAGGCCACTCACCACAACCCAATCCAAAGGCACCGACGACTCCGTCGACGCCCCCGACCGCCTCATCTCCGCCGTCTGCTACTTCTACCCCTTCTTCGACGGCATCCAGTACGGAAAATACGTGATTACCCAGTTCACCGCCATCCAAACCCTCATCCAGCCGTTGATTCCAGCCATAAGGGTCTTCAAGAGCTTCCCCTTCAATGGGTTTTTGGTATTTTTAACCCTTTACTTTGGGGTGGTTAGAAACTCAAATTTTAGCAGATATGTGAGGTTCAATACTATGCAAGTGATTGTGCTTGATGTGCTGTTGATTTTTCCGGATTTGTTGGAGAGGAGCTTTAACCCGAAAGATGGGTTGGGATTGGATGTGCTGATGAGCATGGATAGTACTGTGTTTCTCTTCCTTTTGGTGTGTTTGGTTTATGGGTCTGCTTCTTGCGTTTTGGGTCAGGTGCCCAGATTGCCCATTGTAGCTGAAGCTGCTGAGAGGCAAGTGCCTTAG

Protein Analysis

248

Amino Acids

27.81

Weight (kDa)

9.45

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 100 - 242 4.5e-53 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AccB1I GGYRCC 2 cut(s) 269, 694
AccI GTMKAC 1 cut(s) 285
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 4 cut(s) 160, 298, 309, 378
AcsI RAATTY 1 cut(s) 493
AcuI CTGAAG 1 cut(s) 738
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 2 cut(s) 350, 631
AfiI CCNNNNNNNGG 3 cut(s) 443, 444, 593
AgsI TTSAA 3 cut(s) 427, 442, 517
AjuI GAANNNNNNNTTGG 2 cut(s) 581, 613
AluBI AGCT 4 cut(s) 432, 579, 716, 722
AluI AGCT 4 cut(s) 432, 579, 716, 722
Aor13HI TCCGGA 1 cut(s) 559
AoxI GGCC 2 cut(s) 100, 242
ApeKI GCWGC 1 cut(s) 722
ApoI RAATTY 1 cut(s) 493
AsuHPI GGTGA 4 cut(s) 118, 124, 241, 367
BaeGI GKGCMC 1 cut(s) 699
BanI GGYRCC 2 cut(s) 269, 694
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 709
BccI CCATC 4 cut(s) 82, 212, 389, 587
BceAI ACGGC 3 cut(s) 296, 355, 385
BisI GCNGC 1 cut(s) 723
BlsI GCNGC 1 cut(s) 724
BmcAI AGTACT 1 cut(s) 631
BmiI GGNNCC 2 cut(s) 271, 696
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 2 cut(s) 28, 351
BmuI ACTGGG 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 415
BsaAI YACGTR 1 cut(s) 361
BsaHI GRCGYC 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 559
BsaXI ACNNNNNCTCC 6 cut(s) 124, 141, 154, 171, 185, 215
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 444, 593
Bse1I ACTGG 3 cut(s) 41, 346, 370
BseAI TCCGGA 1 cut(s) 559
BseGI GGATG 5 cut(s) 204, 342, 381, 393, 613
BseLI CCNNNNNNNGG 3 cut(s) 443, 444, 593
BseMII CTCAG 1 cut(s) 717
BseNI ACTGG 3 cut(s) 41, 346, 370
BseRI GAGGAG 3 cut(s) 23, 146, 589
BseSI GKGCMC 1 cut(s) 699
BseXI GCAGC 1 cut(s) 709
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 2 cut(s) 102, 244
BshNI GGYRCC 2 cut(s) 269, 694
BsiEI CGRYCG 1 cut(s) 298
BsiSI CCGG 2 cut(s) 99, 560
BslI CCNNNNNNNGG 3 cut(s) 443, 444, 593
BsnI GGCC 2 cut(s) 102, 244
Bsp1286I GDGCHC 1 cut(s) 699
Bsp13I TCCGGA 1 cut(s) 559
BspACI CCGC 4 cut(s) 160, 298, 309, 378
BspANI GGCC 2 cut(s) 102, 244
BspCNI CTCAG 1 cut(s) 718
BspEI TCCGGA 1 cut(s) 559
BspLI GGNNCC 2 cut(s) 271, 696
BspT107I GGYRCC 2 cut(s) 269, 694
BsrI ACTGG 3 cut(s) 41, 346, 370
BssNI GRCGYC 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 130, 634
Bst6I CTCTTC 3 cut(s) 33, 185, 647
BstACI GRCGYC 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 361
BstDEI CTNAG 2 cut(s) 726, 741
BstF5I GGATG 5 cut(s) 204, 342, 381, 393, 613
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 3 cut(s) 544, 703, 722
BstSLI GKGCMC 1 cut(s) 699
BstV1I GCAGC 1 cut(s) 709
BstV2I GAAGAC 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 88
BsuRI GGCC 2 cut(s) 102, 244
BtsCI GGATG 5 cut(s) 204, 342, 381, 393, 613
CseI GACGC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 349, 630
CviAII CATG 1 cut(s) 622
CviJI RGCY 9 cut(s) 92, 102, 244, 400, 413, 432, 579, 716, 722
CviKI_1 RGCY 9 cut(s) 92, 102, 244, 400, 413, 432, 579, 716, 722
CviQI GTAC 2 cut(s) 349, 630
DdeI CTNAG 2 cut(s) 726, 741
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 3 cut(s) 33, 185, 647
EarI CTCTTC 3 cut(s) 33, 185, 647
EciI GGCGGA 2 cut(s) 149, 298
Eco57I CTGAAG 1 cut(s) 738
FaeI CATG 1 cut(s) 625
FaiI YATR 6 cut(s) 95, 416, 507, 524, 623, 666
FatI CATG 1 cut(s) 621
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 723
FokI GGATG 5 cut(s) 191, 329, 368, 380, 620
Fsp4HI GCNGC 1 cut(s) 723
GluI GCNGC 1 cut(s) 723
HaeIII GGCC 2 cut(s) 102, 244
HapII CCGG 2 cut(s) 99, 560
HgaI GACGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 1 cut(s) 625
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 2 cut(s) 278, 406
HpaII CCGG 2 cut(s) 99, 560
HphI GGTGA 4 cut(s) 118, 124, 241, 367
Hpy166II GTNNAC 3 cut(s) 46, 286, 375
Hpy188III TCNNGA 2 cut(s) 427, 560
Hpy8I GTNNAC 3 cut(s) 46, 286, 375
Hpy99I CGWCG 4 cut(s) 278, 287, 290, 341
HpyAV CCTTC 5 cut(s) 113, 184, 234, 340, 448
HpyCH4III ACNGT 2 cut(s) 130, 634
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 1 cut(s) 526
HpyF10VI GCNNNNNNNGC 3 cut(s) 544, 703, 722
HpyF3I CTNAG 2 cut(s) 726, 741
HpySE526I ACGT 1 cut(s) 360
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 1 cut(s) 625
Kpn2I TCCGGA 1 cut(s) 559
LmnI GCTCC 1 cut(s) 576
Lsp1109I GCAGC 1 cut(s) 709
LweI GCATC 2 cut(s) 28, 351
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 130
MboII GAAGA 5 cut(s) 20, 172, 325, 415, 634
MhlI GDGCHC 1 cut(s) 699
MluCI AATT 1 cut(s) 493
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 549
MroI TCCGGA 1 cut(s) 559
MseI TTAA 2 cut(s) 461, 582
MspI CCGG 2 cut(s) 99, 560
MwoI GCNNNNNNNGC 3 cut(s) 544, 703, 722
NlaIII CATG 1 cut(s) 625
NlaIV GGNNCC 2 cut(s) 271, 696
NmuCI GTSAC 1 cut(s) 130
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 291, 357
PfeI GAWTC 1 cut(s) 406
PflFI GACNNNGTC 1 cut(s) 281
PkrI GCNGC 1 cut(s) 724
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 271, 696
PsyI GACNNNGTC 1 cut(s) 281
RsaI GTAC 2 cut(s) 350, 631
RsaNI GTAC 2 cut(s) 349, 630
SalI GTCGAC 1 cut(s) 284
SaqAI TTAA 2 cut(s) 461, 582
SatI GCNGC 1 cut(s) 723
ScaI AGTACT 1 cut(s) 631
SchI GAGTC 1 cut(s) 272
SduI GDGCHC 1 cut(s) 699
SetI ASST 8 cut(s) 65, 363, 434, 515, 581, 696, 718, 724
SfaNI GCATC 2 cut(s) 28, 351
SgrDI CGTCGACG 1 cut(s) 284
Sse9I AATT 1 cut(s) 493
SsiI CCGC 4 cut(s) 160, 298, 309, 378
TaaI ACNGT 2 cut(s) 130, 634
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 285, 336
TasI AATT 1 cut(s) 493
TatI WGTACW 1 cut(s) 629
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 2 cut(s) 461, 582
Tru9I TTAA 2 cut(s) 461, 582
TseFI GTSAC 1 cut(s) 130
TseI GCWGC 1 cut(s) 722
Tsp45I GTSAC 1 cut(s) 130
TspDTI ATGAA 2 cut(s) 56, 61
TspGWI ACGGA 2 cut(s) 271, 366
Tth111I GACNNNGTC 1 cut(s) 281
XapI RAATTY 1 cut(s) 493
XmiI GTMKAC 1 cut(s) 285
ZrmI AGTACT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.