AT5G55900

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007599
Physical Location & Seq
Forward (+)
22637510 .. 22639828
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55900.1

Sequence Viewer

Length: 1242 bp
ATGGGAAGCGGAAGATATCTAGACGACCCTTTAACATTTACTAGAAACCCACCTTCCTCGTCGTCTCCAATCACCGAATCTAGCTTTCTCGCAGAATCGATCTCTCGCTCTGGTAGCTTCGAGAGTGGGAGCTTACGAGGAGGCGATGGTGATTGCTTTAGCGACGTCGATTTCGCACTTGATAAACTCGCTGGGACTGTACAGTTCTACGAGAGGCATGTCTTCTTGTGCTACAAAAAACCATCCGTTTGGCCTGCGAGGATCGAAGCTTCTGAGTTTGATCGGTTACCGAGGCTTCTCTCCTCCGTTATATCGGCGAGGAAGAGTAGTATGAAGAAAGAGACTTTACTCACAATATGTGAGGGACATGATGGTTCAGAGACATCAAATGGGGATGTTCTGATATTTCCAGATATGATCAGATACAGAAGGTTGACTCATTTTGATGTGGACACATTTGTTGAGGAGGTGCTTGTGAAGGGTGTTGAGTGGTTGCCTGGAAATCCTGAATCTCTTAGTAGTTCATATGTCTTTGTATGTTGTCATGGATCTCGAGACCGGCGATGTGGGGTTTGTGGACCTTCTCTAGTTAGTCGATTCAGAGAAGAAATTGACTCGTGTGGTCTTCGAGGTGAAGTTTCAGTCAGCCCTTGTTCTCACATTGGTGGTCACAAATACACTGGAGATGTTATCATCTATGGATTAAATATCAACCAGAGAGTCACAGGACACTGGTATGGATGTGTGACTCTAGAGGATGTTCCTCTTTTGTTGGAGCAACACATTAACAAAGGCGAAATTGTAGACCGGCTTTGGAGGGGTGAAATGGGTCTGCCAGAAGAAGATCAGAAGAAAACTCAAGAACAAAGGTTACAATTAAACAGCGAAAAGATCAGCAACAGAGAGGTGACTCAAGAATCAGTTAATAATAGTATCTGTTGCCAGTCTCGAGCTGTCCCAGAGCTGAACGGTAGTGGTTGCCAACAAAATGGGAACTCCTCTTACTGTCTGGAGGAGATTCACACAGAGAAAAATACCTCGGAGAGAGTAACCTCTGTAAAAAATGCATCTCTTCGGATAGGAAGCAGCGAGAATGGATCTTCTGGTGGATTCAAGGTTTGTGCGGTGATGTCAATGTGGTTGGAGACTTGGGAGAGAGAAGATACATATGCTGCTTTAGCTGTTGCTTGTGCTGCTGCATCTGTGGCTATTGCTTATAACTGCTATAAACAATTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

413

Amino Acids

45.73

Weight (kDa)

5.44

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 63 - 274 4.2e-44 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1218
AatII GACGTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 804
AciI CCGC 2 cut(s) 9, 1124
AclWI GGATC 3 cut(s) 269, 556, 1105
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 201
AgsI TTSAA 2 cut(s) 1114, 1237
AjnI CCWGG 1 cut(s) 496
AleI CACNNNNGTG 1 cut(s) 663
AluBI AGCT 7 cut(s) 84, 117, 132, 269, 953, 964, 1181
AluI AGCT 7 cut(s) 84, 117, 132, 269, 953, 964, 1181
Alw26I GTCTC 6 cut(s) 69, 335, 374, 549, 951, 1139
AlwI GGATC 3 cut(s) 269, 556, 1105
Ama87I CYCGRG 2 cut(s) 552, 948
AoxI GGCC 1 cut(s) 251
ApeKI GCWGC 4 cut(s) 1086, 1172, 1193, 1196
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AspS9I GGNCC 1 cut(s) 578
AsuHPI GGTGA 6 cut(s) 64, 161, 644, 833, 919, 1138
AvaI CYCGRG 2 cut(s) 552, 948
AvaII GGWCC 1 cut(s) 578
BauI CACGAG 1 cut(s) 616
BbsI GAAGAC 2 cut(s) 214, 617
BbvI GCAGC 4 cut(s) 1098, 1159, 1180, 1183
BccI CCATC 3 cut(s) 140, 250, 365
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 1 cut(s) 417
BcoDI GTCTC 6 cut(s) 69, 335, 374, 549, 951, 1139
BfaI CTAG 5 cut(s) 20, 42, 81, 587, 752
BisI GCNGC 4 cut(s) 1087, 1173, 1194, 1197
BlsI GCNGC 4 cut(s) 1088, 1174, 1195, 1198
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 578
BmeT110I CYCGRG 2 cut(s) 552, 948
BmgT120I GGNCC 1 cut(s) 578
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 2 cut(s) 1076, 1208
BpiI GAAGAC 2 cut(s) 214, 617
BpmI CTGGAG 2 cut(s) 702, 1031
BpuEI CTTGAG 2 cut(s) 843, 897
Bsa29I ATCGAT 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 290, 1038
Bse118I RCCGGY 2 cut(s) 558, 807
Bse1I ACTGG 3 cut(s) 685, 737, 943
BseBI CCWGG 1 cut(s) 498
BseCI ATCGAT 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 290, 1038
BseGI GGATG 4 cut(s) 242, 400, 746, 763
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 3 cut(s) 685, 737, 943
BseRI GAGGAG 5 cut(s) 153, 292, 479, 988, 1028
BseXI GCAGC 4 cut(s) 1098, 1159, 1180, 1183
BseYI CCCAGC 1 cut(s) 191
BshFI GGCC 1 cut(s) 253
BshVI ATCGAT 1 cut(s) 98
BsiHKCI CYCGRG 2 cut(s) 552, 948
BsiSI CCGG 2 cut(s) 559, 808
BslFI GGGAC 3 cut(s) 208, 378, 941
BsmAI GTCTC 6 cut(s) 69, 335, 374, 549, 951, 1139
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 3 cut(s) 208, 378, 941
BsnI GGCC 1 cut(s) 253
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 2 cut(s) 552, 948
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
BspACI CCGC 2 cut(s) 9, 1124
BspANI GGCC 1 cut(s) 253
BspCNI CTCAG 1 cut(s) 265
BspDI ATCGAT 1 cut(s) 98
BspPI GGATC 3 cut(s) 269, 556, 1105
BspTNI GGTCTC 1 cut(s) 549
BsrFI RCCGGY 2 cut(s) 558, 807
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 3 cut(s) 685, 737, 943
BssAI RCCGGY 2 cut(s) 558, 807
BssECI CCNNGG 2 cut(s) 290, 1038
BssMI GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 1 cut(s) 616
Bst2BI CACGAG 1 cut(s) 616
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 4 cut(s) 199, 204, 971, 1007
Bst6I CTCTTC 2 cut(s) 317, 1077
BstACI GRCGYC 1 cut(s) 165
BstAUI TGTACA 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 255
BstDEI CTNAG 2 cut(s) 273, 515
BstEII GGTNACC 1 cut(s) 285
BstF5I GGATG 4 cut(s) 242, 400, 746, 763
BstKTI GATC 8 cut(s) 102, 264, 283, 420, 551, 847, 894, 1100
BstMAI GTCTC 6 cut(s) 69, 335, 374, 549, 951, 1139
BstMBI GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
BstMWI GCNNNNNNNGC 4 cut(s) 114, 1178, 1193, 1205
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 1 cut(s) 221
BstPI GGTNACC 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 496
BstV1I GCAGC 4 cut(s) 1098, 1159, 1180, 1183
BstV2I GAAGAC 2 cut(s) 214, 617
BstX2I RGATCY 2 cut(s) 548, 1097
BstXI CCANNNNNNTGG 2 cut(s) 249, 989
BstYI RGATCY 2 cut(s) 548, 1097
Bsu15I ATCGAT 1 cut(s) 98
BsuRI GGCC 1 cut(s) 253
BsuTUI ATCGAT 1 cut(s) 98
BtgZI GCGATG 2 cut(s) 159, 577
BtsCI GGATG 4 cut(s) 242, 400, 746, 763
BtsIMutI CAGTG 2 cut(s) 678, 730
Cac8I GCNNGC 1 cut(s) 255
Cfr10I RCCGGY 2 cut(s) 558, 807
Cfr13I GGNCC 1 cut(s) 578
ClaI ATCGAT 1 cut(s) 98
Csp6I GTAC 1 cut(s) 200
CviAII CATG 3 cut(s) 218, 368, 545
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 2 cut(s) 273, 515
DpnI GATC 8 cut(s) 101, 263, 282, 419, 550, 846, 893, 1099
DpnII GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
Eam1104I CTCTTC 2 cut(s) 317, 1077
EarI CTCTTC 2 cut(s) 317, 1077
Eco31I GGTCTC 1 cut(s) 549
Eco32I GATATC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 578
Eco88I CYCGRG 2 cut(s) 552, 948
Eco91I GGTNACC 1 cut(s) 285
EcoO65I GGTNACC 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 496
EcoRV GATATC 1 cut(s) 17
EcoT22I ATGCAT 1 cut(s) 1069
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 3 cut(s) 221, 371, 548
FaqI GGGAC 3 cut(s) 208, 378, 941
FatI CATG 3 cut(s) 217, 367, 544
FauNDI CATATG 2 cut(s) 526, 1168
FbaI TGATCA 1 cut(s) 417
FblI GTMKAC 1 cut(s) 804
Fnu4HI GCNGC 4 cut(s) 1087, 1173, 1194, 1197
FokI GGATG 4 cut(s) 229, 407, 753, 770
Fsp4HI GCNGC 4 cut(s) 1087, 1173, 1194, 1197
FspBI CTAG 5 cut(s) 20, 42, 81, 587, 752
GluI GCNGC 4 cut(s) 1087, 1173, 1194, 1197
GsaI CCCAGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 702, 1031
HaeIII GGCC 1 cut(s) 253
HapII CCGG 2 cut(s) 559, 808
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 3 cut(s) 221, 371, 548
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HindIII AAGCTT 1 cut(s) 267
HpaII CCGG 2 cut(s) 559, 808
HphI GGTGA 6 cut(s) 64, 161, 644, 833, 919, 1138
Hpy166II GTNNAC 4 cut(s) 435, 451, 578, 805
Hpy188I TCNGA 8 cut(s) 274, 379, 402, 422, 602, 849, 1042, 1077
Hpy8I GTNNAC 4 cut(s) 435, 451, 578, 805
Hpy99I CGWCG 3 cut(s) 64, 167, 170
HpyAV CCTTC 4 cut(s) 63, 423, 472, 591
HpyCH4III ACNGT 4 cut(s) 199, 204, 971, 1007
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 2 cut(s) 1067, 1199
HpyF10VI GCNNNNNNNGC 4 cut(s) 114, 1178, 1193, 1205
HpyF3I CTNAG 2 cut(s) 273, 515
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 3 cut(s) 221, 371, 548
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
LmnI GCTCC 2 cut(s) 129, 775
Lsp1109I GCAGC 4 cut(s) 1098, 1159, 1180, 1183
LweI GCATC 2 cut(s) 1076, 1208
MaeI CTAG 5 cut(s) 20, 42, 81, 587, 752
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 7 cut(s) 285, 668, 721, 745, 870, 907, 1048
MalI GATC 8 cut(s) 101, 263, 282, 419, 550, 846, 893, 1099
MboI GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
MfeI CAATTG 1 cut(s) 1232
MflI RGATCY 2 cut(s) 548, 1097
MluCI AATT 4 cut(s) 609, 798, 875, 1232
MlyI GAGTC 5 cut(s) 430, 608, 729, 742, 904
MmeI TCCRAC 2 cut(s) 753, 1122
Mph1103I ATGCAT 1 cut(s) 1069
MseI TTAA 5 cut(s) 32, 704, 786, 878, 924
MslI CAYNNNNRTG 3 cut(s) 444, 663, 735
MspI CCGG 2 cut(s) 559, 808
MspR9I CCNGG 1 cut(s) 498
MunI CAATTG 1 cut(s) 1232
MvaI CCWGG 1 cut(s) 498
MwoI GCNNNNNNNGC 4 cut(s) 114, 1178, 1193, 1205
NdeI CATATG 2 cut(s) 526, 1168
NdeII GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
NlaIII CATG 3 cut(s) 221, 371, 548
NmuCI GTSAC 4 cut(s) 668, 721, 745, 907
NsiI ATGCAT 1 cut(s) 1069
NspI RCATGY 1 cut(s) 221
OliI CACNNNNGTG 1 cut(s) 663
PaeR7I CTCGAG 2 cut(s) 552, 948
PcsI WCGNNNNNNNCGW 1 cut(s) 559
PfeI GAWTC 7 cut(s) 77, 95, 509, 597, 917, 1018, 1110
PkrI GCNGC 4 cut(s) 1088, 1174, 1195, 1198
PleI GAGTC 5 cut(s) 430, 608, 728, 742, 904
PpsI GAGTC 5 cut(s) 430, 608, 728, 742, 904
PsiI TTATAA 1 cut(s) 1218
Psp6I CCWGG 1 cut(s) 496
PspEI GGTNACC 1 cut(s) 285
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 1 cut(s) 496
PspPI GGNCC 1 cut(s) 578
PsrI GAACNNNNNNTAC 4 cut(s) 855, 887, 986, 1018
PsuI RGATCY 2 cut(s) 548, 1097
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 3 cut(s) 444, 663, 735
SaqAI TTAA 5 cut(s) 32, 704, 786, 878, 924
SatI GCNGC 4 cut(s) 1087, 1173, 1194, 1197
Sau3AI GATC 8 cut(s) 99, 261, 280, 417, 548, 844, 891, 1097
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 5 cut(s) 430, 608, 729, 742, 904
ScrFI CCNGG 1 cut(s) 498
SfaNI GCATC 2 cut(s) 1076, 1208
Sfr274I CTCGAG 2 cut(s) 552, 948
SinI GGWCC 1 cut(s) 578
SlaI CTCGAG 2 cut(s) 552, 948
SmiMI CAYNNNNRTG 3 cut(s) 444, 663, 735
SmlI CTYRAG 4 cut(s) 552, 858, 912, 948
SmoI CTYRAG 4 cut(s) 552, 858, 912, 948
Sse9I AATT 4 cut(s) 609, 798, 875, 1232
SsiI CCGC 2 cut(s) 9, 1124
SspMI CTAG 5 cut(s) 20, 42, 81, 587, 752
StyD4I CCNGG 1 cut(s) 496
TaaI ACNGT 4 cut(s) 199, 204, 971, 1007
TaiI ACGT 1 cut(s) 168
TaqI TCGA 8 cut(s) 98, 120, 168, 264, 553, 595, 628, 949
TasI AATT 4 cut(s) 609, 798, 875, 1232
TatI WGTACW 1 cut(s) 199
TfiI GAWTC 7 cut(s) 77, 95, 509, 597, 917, 1018, 1110
Tru1I TTAA 5 cut(s) 32, 704, 786, 878, 924
Tru9I TTAA 5 cut(s) 32, 704, 786, 878, 924
TscAI CASTG 2 cut(s) 685, 737
TseFI GTSAC 4 cut(s) 668, 721, 745, 907
TseI GCWGC 4 cut(s) 1086, 1172, 1193, 1196
Tsp45I GTSAC 4 cut(s) 668, 721, 745, 907
TspDTI ATGAA 2 cut(s) 347, 513
TspGWI ACGGA 2 cut(s) 235, 295
TspRI CASTG 2 cut(s) 685, 737
VpaK11BI GGWCC 1 cut(s) 578
XbaI TCTAGA 2 cut(s) 19, 751
XceI RCATGY 1 cut(s) 221
XhoI CTCGAG 2 cut(s) 552, 948
XmiI GTMKAC 1 cut(s) 804
XspI CTAG 5 cut(s) 20, 42, 81, 587, 752
ZraI GACGTC 1 cut(s) 166
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.