Rroxscaffold_1G00005430

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007599
Physical Location & Seq
Forward (+)
7420670 .. 7424128
3459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005430.1

Sequence Viewer

Length: 1242 bp
ATGGCTAGCGCCCGAGACGACTCCTTGCTCCCATTTACGACGCCCCCGTCCACGTCGTCTTCCCCGATTGTGATATCCGACCCGCTCGACGGGTTCCTTTCCGACCCGAATTCCCACATCGGGACCAGCGCCTCCGGGAGCTTCCAGAACGAGGGTTTGTTGGCCGATACTAGCGCGAGCAGCAGCGACGCCGAGTTTGGGTTTTCGAGGGGCGAGTTCCGGACGAGTCAACTCGCCGGGACGGTGGAGTTTTATCAGAGGCATGTGTTTTTGTGCTATAAGAACCCGCAGGTGTGGCCGCCGAGGATTGAGGCGGCGGAGTTTGATCGGCTGCCGAGGCTGCTCCACGCGGCGGTGATGGCGAGGAGGGTTGATATGAGCAAAGAGACTTGGTGGAAACATTGCAGGAGATTGACACATTTTGATGTTGACACATTCGTTGAAGAAGTTCTAGTGAAGGATGGTGAGTGGCTGCCTGGAACTCCTGAAACTCTGAGGGGTTCATACATATTTGTATGTTCTCATGGGTCCAGGGATCGCCGTTGTGGAGTCTGTGGGCCTCCCTTGATCAATAGATTCAGAGAAGAGATAGATTTGCATGGTCTTAAAGGTAAAGTGTCTGTTAGGCCATGTTCACACATTGGGGGGCATAAGTATGCAGGCAATGTAATTATATTTGGATCAAATATCAACAAAGAAGTCACTGGCCACTGGTATGGATATGTTGCTCCAGAAGATGTACCTCAATTGCTTGAGCAGCATATTGGGAGAGGAGAAATTGTAGACTGGCTATGGAGGGGTCAAATGGGTTTATCAGAAGAACAACAGAAGAAATCTCAAGAACTAAGGCTCCAGGCTAATGGCGAGTCAAATGTGGAGAAAAGCAGTACAGAGGTGACACAAACGAAGGAAACGGGGATGAATACTGGTCCATGCAGATCTCAAGTTGAAATTGGGGGCTGTTGCGAGGAAAACGGAAGCTCTTCTTGCTGCCAGAACACTTCGTTGACAGATAGGATAAGTAGTCCTGATTTGAATATGATGGCAACACAAGCGACATCTGAAAAGAAAAAAGCAGTAGCAAACTACTTTCACGCAGAAGCAAAGCTGCATCTGCTCGAAAGTTTTGTGCTATGCCAACATGGTCTGAGAGCTGGGAGCGGGAAGATACATATGCAGCTTTGGCTATTGTTTGTGCTGCTGTGTCAGTTGGTGTTGCTTACAGTTGCTACAGACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

413

Amino Acids

45.81

Weight (kDa)

6.02

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 78 - 267 1.1e-37 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 280
AasI GACNNNNNNGTC 1 cut(s) 46
Acc36I ACCTGC 1 cut(s) 280
AccBSI CCGCTC 2 cut(s) 85, 1161
AccI GTMKAC 1 cut(s) 783
AccII CGCG 2 cut(s) 176, 350
AccIII TCCGGA 1 cut(s) 219
AciI CCGC 8 cut(s) 83, 287, 299, 314, 317, 350, 353, 1161
AclWI GGATC 2 cut(s) 543, 688
AcoI YGGCCR 3 cut(s) 162, 296, 706
AcsI RAATTY 1 cut(s) 109
AcyI GRCGYC 2 cut(s) 41, 189
AfaI GTAC 2 cut(s) 741, 889
AfiI CCNNNNNNNGG 5 cut(s) 89, 120, 151, 198, 352
AgsI TTSAA 3 cut(s) 443, 950, 1036
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 3 cut(s) 475, 530, 852
AloI GAACNNNNNNTCC 2 cut(s) 834, 866
AluBI AGCT 5 cut(s) 141, 981, 1108, 1154, 1180
AluI AGCT 5 cut(s) 141, 981, 1108, 1154, 1180
Alw26I GTCTC 2 cut(s) 9, 380
AlwI GGATC 2 cut(s) 543, 688
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 219
AoxI GGCC 5 cut(s) 162, 296, 557, 626, 706
ApoI RAATTY 1 cut(s) 109
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
Asp700I GAANNNNTTC 2 cut(s) 447, 982
AspLEI GCGC 3 cut(s) 11, 131, 176
AspS9I GGNCC 4 cut(s) 123, 528, 557, 929
AsuC2I CCSGG 2 cut(s) 136, 238
AsuHPI GGTGA 3 cut(s) 367, 476, 907
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 3 cut(s) 123, 528, 929
BalI TGGCCA 1 cut(s) 708
BbsI GAAGAC 1 cut(s) 51
BccI CCATC 3 cut(s) 352, 455, 1036
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 3 cut(s) 477, 532, 854
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 2 cut(s) 136, 238
BcoDI GTCTC 2 cut(s) 9, 380
BfaI CTAG 3 cut(s) 6, 171, 452
BfmI CTRYAG 1 cut(s) 1230
BfoI RGCGCY 2 cut(s) 12, 132
BfuAI ACCTGC 1 cut(s) 280
BglII AGATCT 1 cut(s) 938
Bme1390I CCNGG 5 cut(s) 136, 238, 477, 532, 854
Bme18I GGWCC 3 cut(s) 123, 528, 929
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 4 cut(s) 123, 528, 557, 929
BmiI GGNNCC 4 cut(s) 95, 124, 529, 851
BmrFI CCNGG 5 cut(s) 136, 238, 477, 532, 854
BmsI GCATC 1 cut(s) 1120
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 2 cut(s) 714, 836
BpuEI CTTGAG 3 cut(s) 773, 822, 927
BpuMI CCSGG 2 cut(s) 136, 238
BsaHI GRCGYC 2 cut(s) 41, 189
BsaJI CCNNGG 3 cut(s) 302, 335, 531
BsaWI WCCGGW 1 cut(s) 219
BsaXI ACNNNNNCTCC 4 cut(s) 765, 795, 834, 864
Bsc4I CCNNNNNNNGG 5 cut(s) 89, 120, 151, 198, 352
Bse1I ACTGG 4 cut(s) 709, 716, 791, 931
Bse3DI GCAATG 2 cut(s) 400, 670
BseAI TCCGGA 1 cut(s) 219
BseBI CCWGG 3 cut(s) 477, 532, 854
BseDI CCNNGG 3 cut(s) 302, 335, 531
BseGI GGATG 2 cut(s) 466, 924
BseLI CCNNNNNNNGG 5 cut(s) 89, 120, 151, 198, 352
BseMI GCAATG 2 cut(s) 400, 670
BseMII CTCAG 2 cut(s) 485, 1139
BseNI ACTGG 4 cut(s) 709, 716, 791, 931
BseRI GAGGAG 2 cut(s) 379, 786
BseYI CCCAGC 1 cut(s) 1154
Bsh1236I CGCG 2 cut(s) 176, 350
BshFI GGCC 5 cut(s) 164, 298, 559, 628, 708
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 3 cut(s) 135, 220, 237
BslFI GGGAC 2 cut(s) 136, 253
BslI CCNNNNNNNGG 5 cut(s) 89, 120, 151, 198, 352
BsmAI GTCTC 2 cut(s) 9, 380
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 136, 253
BsnI GGCC 5 cut(s) 164, 298, 559, 628, 708
BsoBI CYCGRG 1 cut(s) 12
Bsp13I TCCGGA 1 cut(s) 219
Bsp143I GATC 5 cut(s) 325, 535, 567, 680, 938
BspACI CCGC 8 cut(s) 83, 287, 299, 314, 317, 350, 353, 1161
BspANI GGCC 5 cut(s) 164, 298, 559, 628, 708
BspCNI CTCAG 2 cut(s) 486, 1140
BspEI TCCGGA 1 cut(s) 219
BspFNI CGCG 2 cut(s) 176, 350
BspLI GGNNCC 4 cut(s) 95, 124, 529, 851
BspMI ACCTGC 1 cut(s) 280
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 543, 688
BspQI GCTCTTC 1 cut(s) 988
BsrBI CCGCTC 2 cut(s) 85, 1161
BsrDI GCAATG 2 cut(s) 400, 670
BsrI ACTGG 4 cut(s) 709, 716, 791, 931
BssECI CCNNGG 3 cut(s) 302, 335, 531
BssMI GATC 5 cut(s) 325, 535, 567, 680, 938
BssNI GRCGYC 2 cut(s) 41, 189
Bst2UI CCWGG 3 cut(s) 477, 532, 854
Bst4CI ACNGT 3 cut(s) 244, 1225, 1238
Bst6I CTCTTC 2 cut(s) 579, 988
BstACI GRCGYC 2 cut(s) 41, 189
BstC8I GCNNGC 3 cut(s) 7, 178, 661
BstDEI CTNAG 3 cut(s) 494, 845, 1148
BstF5I GGATG 2 cut(s) 466, 924
BstFNI CGCG 2 cut(s) 176, 350
BstH2I RGCGCY 2 cut(s) 12, 132
BstHHI GCGC 3 cut(s) 11, 131, 176
BstKTI GATC 5 cut(s) 328, 538, 570, 683, 941
BstMAI GTCTC 2 cut(s) 9, 380
BstMBI GATC 5 cut(s) 325, 535, 567, 680, 938
BstNI CCWGG 3 cut(s) 477, 532, 854
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 5 cut(s) 134, 236, 475, 530, 852
BstSFI CTRYAG 1 cut(s) 1230
BstUI CGCG 2 cut(s) 176, 350
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 938
BstXI CCANNNNNNTGG 2 cut(s) 716, 860
BstYI RGATCY 1 cut(s) 938
BsuRI GGCC 5 cut(s) 164, 298, 559, 628, 708
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 2 cut(s) 466, 924
BtsIMutI CAGTG 2 cut(s) 702, 709
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 3 cut(s) 7, 178, 661
CfoI GCGC 3 cut(s) 11, 131, 176
Cfr13I GGNCC 4 cut(s) 123, 528, 557, 929
CseI GACGC 2 cut(s) 49, 197
Csp6I GTAC 2 cut(s) 740, 888
CviAII CATG 6 cut(s) 263, 524, 599, 630, 933, 1142
CviQI GTAC 2 cut(s) 740, 888
DdeI CTNAG 3 cut(s) 494, 845, 1148
DpnI GATC 5 cut(s) 327, 537, 569, 682, 940
DpnII GATC 5 cut(s) 325, 535, 567, 680, 938
DrdI GACNNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 46
EaeI YGGCCR 3 cut(s) 162, 296, 706
Eam1104I CTCTTC 2 cut(s) 579, 988
EarI CTCTTC 2 cut(s) 579, 988
EciI GGCGGA 1 cut(s) 332
Eco32I GATATC 1 cut(s) 75
Eco47I GGWCC 3 cut(s) 123, 528, 929
Eco88I CYCGRG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 109
EcoRII CCWGG 3 cut(s) 475, 530, 852
EcoRV GATATC 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 6 cut(s) 266, 527, 602, 633, 936, 1145
FalI AAGNNNNNCTT 2 cut(s) 970, 1002
FaqI GGGAC 2 cut(s) 136, 253
FatI CATG 6 cut(s) 262, 523, 598, 629, 932, 1141
FauI CCCGC 3 cut(s) 90, 294, 1154
FauNDI CATATG 1 cut(s) 1173
FbaI TGATCA 1 cut(s) 567
FblI GTMKAC 1 cut(s) 783
FokI GGATG 2 cut(s) 473, 931
FspBI CTAG 3 cut(s) 6, 171, 452
GlaI GCGC 3 cut(s) 10, 130, 175
GsaI CCCAGC 1 cut(s) 1158
GsuI CTGGAG 2 cut(s) 714, 836
HaeII RGCGCY 2 cut(s) 12, 132
HaeIII GGCC 5 cut(s) 164, 298, 559, 628, 708
HapII CCGG 3 cut(s) 135, 220, 237
HgaI GACGC 2 cut(s) 49, 197
HhaI GCGC 3 cut(s) 11, 131, 176
Hin1I GRCGYC 2 cut(s) 41, 189
Hin1II CATG 6 cut(s) 266, 527, 602, 633, 936, 1145
Hin6I GCGC 3 cut(s) 9, 129, 174
HinP1I GCGC 3 cut(s) 9, 129, 174
HincII GTYRAC 3 cut(s) 230, 430, 1008
HindII GTYRAC 3 cut(s) 230, 430, 1008
HinfI GANTC 5 cut(s) 20, 226, 549, 576, 866
HpaII CCGG 3 cut(s) 135, 220, 237
HphI GGTGA 3 cut(s) 367, 476, 907
Hpy166II GTNNAC 6 cut(s) 51, 230, 430, 635, 784, 1008
Hpy188I TCNGA 8 cut(s) 79, 103, 258, 495, 581, 817, 1063, 1149
Hpy188III TCNNGA 7 cut(s) 121, 145, 220, 485, 731, 839, 1028
Hpy8I GTNNAC 6 cut(s) 51, 230, 430, 635, 784, 1008
Hpy99I CGWCG 4 cut(s) 43, 58, 92, 191
HpyAV CCTTC 2 cut(s) 451, 901
HpyCH4III ACNGT 3 cut(s) 244, 1225, 1238
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 6 cut(s) 405, 598, 659, 936, 1111, 1177
HpyF3I CTNAG 3 cut(s) 494, 845, 1148
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 2 cut(s) 41, 189
Hsp92II CATG 6 cut(s) 266, 527, 602, 633, 936, 1145
HspAI GCGC 3 cut(s) 9, 129, 174
Kpn2I TCCGGA 1 cut(s) 219
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 5 cut(s) 325, 535, 567, 680, 938
LguI GCTCTTC 1 cut(s) 988
LmnI GCTCC 6 cut(s) 33, 138, 348, 733, 855, 1158
LweI GCATC 1 cut(s) 1120
MaeI CTAG 3 cut(s) 6, 171, 452
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 700, 895
MalI GATC 5 cut(s) 327, 537, 569, 682, 940
MbiI CCGCTC 2 cut(s) 85, 1161
MboI GATC 5 cut(s) 325, 535, 567, 680, 938
MboII GAAGA 8 cut(s) 51, 455, 596, 746, 830, 841, 975, 1177
MfeI CAATTG 1 cut(s) 746
MflI RGATCY 1 cut(s) 938
MlsI TGGCCA 1 cut(s) 708
MluCI AATT 5 cut(s) 109, 669, 746, 777, 951
MluNI TGGCCA 1 cut(s) 708
MlyI GAGTC 4 cut(s) 14, 235, 558, 875
MmeI TCCRAC 2 cut(s) 102, 126
Mox20I TGGCCA 1 cut(s) 708
MroI TCCGGA 1 cut(s) 219
MroXI GAANNNNTTC 2 cut(s) 447, 982
MscI TGGCCA 1 cut(s) 708
MseI TTAA 1 cut(s) 606
MslI CAYNNNNRTG 3 cut(s) 423, 654, 714
Msp20I TGGCCA 1 cut(s) 708
MspI CCGG 3 cut(s) 135, 220, 237
MspR9I CCNGG 5 cut(s) 136, 238, 477, 532, 854
MunI CAATTG 1 cut(s) 746
MvaI CCWGG 3 cut(s) 477, 532, 854
MvnI CGCG 2 cut(s) 176, 350
NciI CCSGG 2 cut(s) 136, 238
NdeI CATATG 1 cut(s) 1173
NdeII GATC 5 cut(s) 325, 535, 567, 680, 938
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 6 cut(s) 266, 527, 602, 633, 936, 1145
NlaIV GGNNCC 4 cut(s) 95, 124, 529, 851
NmeAIII GCCGAG 3 cut(s) 217, 327, 360
NmuCI GTSAC 2 cut(s) 700, 895
NspI RCATGY 1 cut(s) 266
PaqCI CACCTGC 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 988
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 62
PdmI GAANNNNTTC 2 cut(s) 447, 982
PfeI GAWTC 1 cut(s) 576
PfoI TCCNGGA 1 cut(s) 134
PleI GAGTC 4 cut(s) 14, 234, 557, 874
PpsI GAGTC 4 cut(s) 14, 234, 557, 874
Psp6I CCWGG 3 cut(s) 475, 530, 852
PspFI CCCAGC 1 cut(s) 1154
PspGI CCWGG 3 cut(s) 475, 530, 852
PspN4I GGNNCC 4 cut(s) 95, 124, 529, 851
PspPI GGNCC 4 cut(s) 123, 528, 557, 929
PsuI RGATCY 1 cut(s) 938
RsaI GTAC 2 cut(s) 741, 889
RsaNI GTAC 2 cut(s) 740, 888
RseI CAYNNNNRTG 3 cut(s) 423, 654, 714
SapI GCTCTTC 1 cut(s) 988
SaqAI TTAA 1 cut(s) 606
Sau3AI GATC 5 cut(s) 325, 535, 567, 680, 938
Sau96I GGNCC 4 cut(s) 123, 528, 557, 929
SchI GAGTC 4 cut(s) 14, 235, 558, 875
ScrFI CCNGG 5 cut(s) 136, 238, 477, 532, 854
SfaNI GCATC 1 cut(s) 1120
SfcI CTRYAG 1 cut(s) 1230
SinI GGWCC 3 cut(s) 123, 528, 929
SmiMI CAYNNNNRTG 3 cut(s) 423, 654, 714
SmlI CTYRAG 3 cut(s) 752, 837, 942
SmoI CTYRAG 3 cut(s) 752, 837, 942
Sse9I AATT 5 cut(s) 109, 669, 746, 777, 951
SsiI CCGC 8 cut(s) 83, 287, 299, 314, 317, 350, 353, 1161
SspMI CTAG 3 cut(s) 6, 171, 452
StyD4I CCNGG 5 cut(s) 134, 236, 475, 530, 852
TaaI ACNGT 3 cut(s) 244, 1225, 1238
TaiI ACGT 1 cut(s) 56
TaqI TCGA 3 cut(s) 87, 206, 1119
TasI AATT 5 cut(s) 109, 669, 746, 777, 951
TatI WGTACW 1 cut(s) 887
TauI GCSGC 3 cut(s) 301, 317, 353
TfiI GAWTC 1 cut(s) 576
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TscAI CASTG 2 cut(s) 709, 716
TseFI GTSAC 2 cut(s) 700, 895
Tsp45I GTSAC 2 cut(s) 700, 895
TspDTI ATGAA 2 cut(s) 492, 935
TspGWI ACGGA 1 cut(s) 990
TspRI CASTG 2 cut(s) 709, 716
VpaK11BI GGWCC 3 cut(s) 123, 528, 929
XapI RAATTY 1 cut(s) 109
XceI RCATGY 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 783
XmnI GAANNNNTTC 2 cut(s) 447, 982
XspI CTAG 3 cut(s) 6, 171, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.