MD03G1029300.v1.1

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007599
Physical Location & Seq
Forward (+)
2389839 .. 2394267
4429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1029300.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGGGGAGCGCCCGAGACCGAGACGACTCACTCGCATTCACCAACCCATCCAAGTCCTCCTCGCCAATCGTCATCTCCGAAGCGCTCGAAAGCTTCCTCTCCGACCCCAATTCCCACATCGGCAGCGCCTCCGGGAGCTTCCAGAACGAGGGTCTCCTCGCCGACACCAGCGGAAGCTGCAGCGACGCCGAGTTCGGGTTCTCCCGGCCCGAGTTCCGGACGAGCCAACTCGCAGGGACGGTGGAGTTTTACCAAAGGCATGTGTTTTTGTGCTACAAGAACCCGCAGGTTTGGCCGCCGAGGATCGAGGCCGCCGAGTTTGATCGGCTGCCGAGGTTGCTCTACTCCGCCGTCATGGCCAGGAGGGGAGATATGAAGAAAGAGACCCGCTTAACAATATGTGAAGGACATGATGGAACTGAAACATCAAATGGCGATGTATTAATTTTCCCCGACATGATCCGATACAGGAGATTGACCCATTTTGATGTTGATACATTTGTTGAAGAAGTTCTTGTGAAGGATGGTGAGTGGCTGCCTGGAACTCCTGAAACTCTGAAGGGGTCATATGTTTTTGTATGTTCTCATGGGTCCCGGGATCGCCGTTGTGGTGTCTGTGGACCTCCCCTGATCAATAGATTTAGAGAAGAGGTAGAATTACATGGTCTTCAGGGTAAAGTGTCCGTTAGCCCATGTTCGCACATTGGGGGGCATAAATATGCTGGAAATGTTATCATGTTTGGATCAAATTGCAACAGAAAAGTATCTGGCCACTGGTATGGATATGTTGCTCCAGAAGATGTACCTGTATTGCTTGAACAGCATGTCGGGAAAGGAGAAATTGTAGACTGGCTATGGAGGGGCCAGATGGGTTTATCAGAAGAACAGCAGAAGGAATCTCAAGAACGAAGGCTCCAGCTTAATGGTGAGACAAATGTGGGAAAAACCACTACAGAGTTGACACAACCAAAGGAAAGGGGGATGAATACTAGTGTATGTAGATCGCAAATCGAGATTGGGGGCTGTTGCCAGGAAAACGGAAACTCTTGCTGTCAGAATGCTACGCTAATGGAAAACATAAATAGTCCCGACCTGAATGAGATTGCAGCGAAGCCAACAGCAGCCGAAAAGAAAAAAAGCAGTAGGAAACCACTCTCACGAATGAATAGTGGCAAAGGGGCATCCACGCGCAAGGTGTGTGCGGTGCCAACATGGTTTCAGACCTGGGAGCGTGAAGATACATACGCAGCTGTAGCTGTTGTTTGTGCCGCTGTGTCAGTTGCCGTTGCTTACAAGTGCTACAAACAGTTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

439

Amino Acids

48.5

Weight (kDa)

6.4

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 77 - 288 1.4e-45 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 1204
AccI GTMKAC 1 cut(s) 846
AccII CGCG 1 cut(s) 1189
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 8 cut(s) 171, 284, 296, 312, 348, 388, 1202, 1269
AclWI GGATC 4 cut(s) 311, 454, 606, 751
AcoI YGGCCR 3 cut(s) 293, 357, 769
AcuI CTGAAG 2 cut(s) 578, 653
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 804
AfeI AGCGCT 1 cut(s) 84
AfiI CCNNNNNNNGG 3 cut(s) 148, 195, 216
AgsI TTSAA 2 cut(s) 506, 818
AhlI ACTAGT 1 cut(s) 989
AjnI CCWGG 4 cut(s) 359, 538, 1029, 1223
AloI GAACNNNNNNTCC 2 cut(s) 897, 929
AluBI AGCT 7 cut(s) 93, 138, 177, 919, 1250, 1256, 1314
AluI AGCT 7 cut(s) 93, 138, 177, 919, 1250, 1256, 1314
Alw26I GTCTC 5 cut(s) 9, 15, 158, 377, 923
AlwI GGATC 4 cut(s) 311, 454, 606, 751
Ama87I CYCGRG 3 cut(s) 12, 209, 594
Aor13HI TCCGGA 1 cut(s) 216
Aor51HI AGCGCT 1 cut(s) 84
AoxI GGCC 6 cut(s) 206, 293, 309, 357, 769, 862
ApeKI GCWGC 8 cut(s) 123, 177, 180, 328, 535, 1106, 1121, 1247
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AseI ATTAAT 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 510
AspLEI GCGC 4 cut(s) 11, 85, 128, 1191
AspS9I GGNCC 4 cut(s) 207, 591, 620, 862
AsuC2I CCSGG 4 cut(s) 133, 205, 595, 596
AsuHPI GGTGA 3 cut(s) 31, 539, 938
AvaI CYCGRG 3 cut(s) 12, 209, 594
AvaII GGWCC 2 cut(s) 591, 620
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BalI TGGCCA 2 cut(s) 359, 771
BanI GGYRCC 1 cut(s) 1204
BbsI GAAGAC 1 cut(s) 659
BbvI GCAGC 8 cut(s) 135, 164, 192, 315, 522, 1118, 1133, 1259
BccI CCATC 4 cut(s) 55, 407, 518, 862
BceAI ACGGC 3 cut(s) 335, 588, 1268
BciT130I CCWGG 4 cut(s) 361, 540, 1031, 1225
BclI TGATCA 1 cut(s) 630
BcnI CCSGG 4 cut(s) 133, 205, 595, 596
BcoDI GTCTC 5 cut(s) 9, 15, 158, 377, 923
BcuI ACTAGT 1 cut(s) 989
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 3 cut(s) 178, 951, 1251
BfoI RGCGCY 3 cut(s) 12, 86, 129
BfuAI ACCTGC 1 cut(s) 277
BglI GCCNNNNNGGC 1 cut(s) 356
Bme1390I CCNGG 8 cut(s) 133, 205, 361, 540, 595, 596, 1031, 1225
Bme18I GGWCC 2 cut(s) 591, 620
BmeT110I CYCGRG 3 cut(s) 12, 209, 594
BmgT120I GGNCC 4 cut(s) 207, 591, 620, 862
BmiI GGNNCC 5 cut(s) 592, 593, 863, 914, 1206
BmrFI CCNGG 8 cut(s) 133, 205, 361, 540, 595, 596, 1031, 1225
BmsI GCATC 1 cut(s) 1190
BpiI GAAGAC 1 cut(s) 659
BpmI CTGGAG 2 cut(s) 777, 899
BpuEI CTTGAG 1 cut(s) 885
BpuMI CCSGG 4 cut(s) 133, 205, 595, 596
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 3 cut(s) 9, 158, 377
BsaJI CCNNGG 4 cut(s) 299, 332, 594, 1224
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 4 cut(s) 828, 858, 897, 927
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 195, 216
Bse1I ACTGG 2 cut(s) 779, 854
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 4 cut(s) 361, 540, 1031, 1225
BseDI CCNNGG 4 cut(s) 299, 332, 594, 1224
BseGI GGATG 4 cut(s) 47, 529, 987, 1181
BseLI CCNNNNNNNGG 3 cut(s) 148, 195, 216
BseNI ACTGG 2 cut(s) 779, 854
BseRI GAGGAG 2 cut(s) 49, 146
BseXI GCAGC 8 cut(s) 135, 164, 192, 315, 522, 1118, 1133, 1259
Bsh1236I CGCG 1 cut(s) 1189
BshFI GGCC 6 cut(s) 208, 295, 311, 359, 771, 864
BshNI GGYRCC 1 cut(s) 1204
BsiHKCI CYCGRG 3 cut(s) 12, 209, 594
BsiSI CCGG 4 cut(s) 132, 205, 217, 595
BslFI GGGAC 3 cut(s) 250, 577, 1071
BslI CCNNNNNNNGG 3 cut(s) 148, 195, 216
BsmAI GTCTC 5 cut(s) 9, 15, 158, 377, 923
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 3 cut(s) 250, 577, 1071
BsmI GAATGC 2 cut(s) 35, 1063
BsnI GGCC 6 cut(s) 208, 295, 311, 359, 771, 864
Bso31I GGTCTC 3 cut(s) 9, 158, 377
BsoBI CYCGRG 3 cut(s) 12, 209, 594
Bsp13I TCCGGA 1 cut(s) 216
Bsp143I GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
BspACI CCGC 8 cut(s) 171, 284, 296, 312, 348, 388, 1202, 1269
BspANI GGCC 6 cut(s) 208, 295, 311, 359, 771, 864
BspEI TCCGGA 1 cut(s) 216
BspFNI CGCG 1 cut(s) 1189
BspLI GGNNCC 5 cut(s) 592, 593, 863, 914, 1206
BspMAI CTGCAG 1 cut(s) 182
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 4 cut(s) 311, 454, 606, 751
BspT107I GGYRCC 1 cut(s) 1204
BspTNI GGTCTC 3 cut(s) 9, 158, 377
BsrI ACTGG 2 cut(s) 779, 854
BssECI CCNNGG 4 cut(s) 299, 332, 594, 1224
BssMI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 4 cut(s) 361, 540, 1031, 1225
Bst4CI ACNGT 2 cut(s) 241, 1308
Bst6I CTCTTC 1 cut(s) 642
BstACI GRCGYC 1 cut(s) 186
BstF5I GGATG 4 cut(s) 47, 529, 987, 1181
BstFNI CGCG 1 cut(s) 1189
BstH2I RGCGCY 3 cut(s) 12, 86, 129
BstHHI GCGC 4 cut(s) 11, 85, 128, 1191
BstKTI GATC 7 cut(s) 306, 325, 462, 601, 633, 746, 1004
BstMAI GTCTC 5 cut(s) 9, 15, 158, 377, 923
BstMBI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
BstMWI GCNNNNNNNGC 6 cut(s) 177, 292, 337, 356, 820, 1253
BstNI CCWGG 4 cut(s) 361, 540, 1031, 1225
BstNSI RCATGY 2 cut(s) 263, 827
BstSCI CCNGG 8 cut(s) 131, 203, 359, 538, 593, 594, 1029, 1223
BstSFI CTRYAG 3 cut(s) 178, 951, 1251
BstUI CGCG 1 cut(s) 1189
BstV1I GCAGC 8 cut(s) 135, 164, 192, 315, 522, 1118, 1133, 1259
BstV2I GAAGAC 1 cut(s) 659
BstXI CCANNNNNNTGG 2 cut(s) 779, 923
BsuRI GGCC 6 cut(s) 208, 295, 311, 359, 771, 864
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 4 cut(s) 47, 529, 987, 1181
BtsIMutI CAGTG 1 cut(s) 772
BveI ACCTGC 1 cut(s) 277
CfoI GCGC 4 cut(s) 11, 85, 128, 1191
Cfr13I GGNCC 4 cut(s) 207, 591, 620, 862
Cfr9I CCCGGG 1 cut(s) 594
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 803
CviQI GTAC 1 cut(s) 803
DpnI GATC 7 cut(s) 305, 324, 461, 600, 632, 745, 1003
DpnII GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
EaeI YGGCCR 3 cut(s) 293, 357, 769
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
EciI GGCGGA 1 cut(s) 337
Eco31I GGTCTC 3 cut(s) 9, 158, 377
Eco47I GGWCC 2 cut(s) 591, 620
Eco47III AGCGCT 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 578, 653
Eco88I CYCGRG 3 cut(s) 12, 209, 594
EcoO109I RGGNCCY 1 cut(s) 591
EcoRII CCWGG 4 cut(s) 359, 538, 1029, 1223
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 3 cut(s) 250, 577, 1071
FauI CCCGC 2 cut(s) 291, 395
FauNDI CATATG 1 cut(s) 568
FbaI TGATCA 1 cut(s) 630
FblI GTMKAC 1 cut(s) 846
FokI GGATG 4 cut(s) 34, 536, 994, 1168
FspBI CTAG 1 cut(s) 990
GlaI GCGC 4 cut(s) 10, 84, 127, 1190
GsuI CTGGAG 2 cut(s) 777, 899
HaeII RGCGCY 3 cut(s) 12, 86, 129
HaeIII GGCC 6 cut(s) 208, 295, 311, 359, 771, 864
HapII CCGG 4 cut(s) 132, 205, 217, 595
HgaI GACGC 1 cut(s) 194
HhaI GCGC 4 cut(s) 11, 85, 128, 1191
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 4 cut(s) 9, 83, 126, 1189
HinP1I GCGC 4 cut(s) 9, 83, 126, 1189
HincII GTYRAC 1 cut(s) 960
HindII GTYRAC 1 cut(s) 960
HindIII AAGCTT 1 cut(s) 91
HinfI GANTC 2 cut(s) 26, 896
HpaII CCGG 4 cut(s) 132, 205, 217, 595
HphI GGTGA 3 cut(s) 31, 539, 938
Hpy166II GTNNAC 3 cut(s) 620, 847, 960
Hpy188I TCNGA 7 cut(s) 79, 103, 464, 558, 880, 1056, 1221
Hpy188III TCNNGA 9 cut(s) 142, 217, 548, 794, 829, 902, 1012, 1088, 1158
Hpy8I GTNNAC 3 cut(s) 620, 847, 960
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 5 cut(s) 398, 514, 553, 886, 903
HpyCH4III ACNGT 2 cut(s) 241, 1308
HpyCH4V TGCA 3 cut(s) 180, 753, 1106
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 292, 337, 356, 820, 1253
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 4 cut(s) 9, 83, 126, 1189
KflI GGGWCCC 1 cut(s) 591
Kpn2I TCCGGA 1 cut(s) 216
Ksp22I TGATCA 1 cut(s) 630
Kzo9I GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
LmnI GCTCC 5 cut(s) 6, 135, 796, 918, 1228
Lsp1109I GCAGC 8 cut(s) 135, 164, 192, 315, 522, 1118, 1133, 1259
LweI GCATC 1 cut(s) 1190
MaeI CTAG 1 cut(s) 990
MalI GATC 7 cut(s) 305, 324, 461, 600, 632, 745, 1003
MboI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
MboII GAAGA 7 cut(s) 388, 518, 659, 659, 809, 893, 1247
MlsI TGGCCA 2 cut(s) 359, 771
MluCI AATT 5 cut(s) 109, 444, 656, 748, 840
MluNI TGGCCA 2 cut(s) 359, 771
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 126
Mox20I TGGCCA 2 cut(s) 359, 771
MroI TCCGGA 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 510
MscI TGGCCA 2 cut(s) 359, 771
MseI TTAA 3 cut(s) 392, 443, 921
MslI CAYNNNNRTG 3 cut(s) 486, 717, 777
Msp20I TGGCCA 2 cut(s) 359, 771
MspA1I CMGCKG 3 cut(s) 171, 1250, 1271
MspI CCGG 4 cut(s) 132, 205, 217, 595
MspR9I CCNGG 8 cut(s) 133, 205, 361, 540, 595, 596, 1031, 1225
Mva1269I GAATGC 2 cut(s) 35, 1063
MvaI CCWGG 4 cut(s) 361, 540, 1031, 1225
MvnI CGCG 1 cut(s) 1189
MwoI GCNNNNNNNGC 6 cut(s) 177, 292, 337, 356, 820, 1253
NciI CCSGG 4 cut(s) 133, 205, 595, 596
NdeI CATATG 1 cut(s) 568
NdeII GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
NlaIV GGNNCC 5 cut(s) 592, 593, 863, 914, 1206
NmeAIII GCCGAG 4 cut(s) 214, 324, 340, 357
NspI RCATGY 2 cut(s) 263, 827
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 312
PctI GAATGC 2 cut(s) 35, 1063
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 1 cut(s) 896
PfoI TCCNGGA 1 cut(s) 131
PleI GAGTC 1 cut(s) 20
PpsI GAGTC 1 cut(s) 20
PpuMI RGGWCCY 1 cut(s) 591
PshBI ATTAAT 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 591
Psp6I CCWGG 4 cut(s) 359, 538, 1029, 1223
PspGI CCWGG 4 cut(s) 359, 538, 1029, 1223
PspN4I GGNNCC 5 cut(s) 592, 593, 863, 914, 1206
PspPI GGNCC 4 cut(s) 207, 591, 620, 862
PspPPI RGGWCCY 1 cut(s) 591
PstI CTGCAG 1 cut(s) 182
PvuII CAGCTG 1 cut(s) 1250
RsaI GTAC 1 cut(s) 804
RsaNI GTAC 1 cut(s) 803
RseI CAYNNNNRTG 3 cut(s) 486, 717, 777
SaqAI TTAA 3 cut(s) 392, 443, 921
Sau3AI GATC 7 cut(s) 303, 322, 459, 598, 630, 743, 1001
Sau96I GGNCC 4 cut(s) 207, 591, 620, 862
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 8 cut(s) 133, 205, 361, 540, 595, 596, 1031, 1225
SfaNI GCATC 1 cut(s) 1190
SfcI CTRYAG 3 cut(s) 178, 951, 1251
SinI GGWCC 2 cut(s) 591, 620
SmaI CCCGGG 1 cut(s) 596
SmiMI CAYNNNNRTG 3 cut(s) 486, 717, 777
SmlI CTYRAG 1 cut(s) 900
SmoI CTYRAG 1 cut(s) 900
SpeI ACTAGT 1 cut(s) 989
Sse9I AATT 5 cut(s) 109, 444, 656, 748, 840
SsiI CCGC 8 cut(s) 171, 284, 296, 312, 348, 388, 1202, 1269
SspMI CTAG 1 cut(s) 990
StyD4I CCNGG 8 cut(s) 131, 203, 359, 538, 593, 594, 1029, 1223
TaaI ACNGT 2 cut(s) 241, 1308
TaqI TCGA 3 cut(s) 87, 306, 1011
TaqII GACCGA 1 cut(s) 33
TasI AATT 5 cut(s) 109, 444, 656, 748, 840
TauI GCSGC 3 cut(s) 298, 314, 1271
TfiI GAWTC 1 cut(s) 896
Tru1I TTAA 3 cut(s) 392, 443, 921
Tru9I TTAA 3 cut(s) 392, 443, 921
TscAI CASTG 1 cut(s) 779
TseI GCWGC 8 cut(s) 123, 177, 180, 328, 535, 1106, 1121, 1247
TspDTI ATGAA 3 cut(s) 389, 998, 1178
TspGWI ACGGA 2 cut(s) 673, 1053
TspMI CCCGGG 1 cut(s) 594
TspRI CASTG 1 cut(s) 779
VpaK11BI GGWCC 2 cut(s) 591, 620
VspI ATTAAT 1 cut(s) 443
XceI RCATGY 2 cut(s) 263, 827
XmaI CCCGGG 1 cut(s) 594
XmiI GTMKAC 1 cut(s) 846
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 1 cut(s) 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.