Rh5AG503100

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007599
Physical Location & Seq
Reverse (-)
85107987 .. 85111072
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG503100.1

Sequence Viewer

Length: 1254 bp
ATGGCTAGCGCCCGAGACCGAGACGACTCCTTGCTCCCATTTACGACGCCCCCATCCACGTCGTCTTCCCCGATTGTGATATCCGACCCGCTCGACGGGTTCCTCTCCGACCCGAATTCCCACATCGGGACCAGCGCCTCCGGGAGCTTCCAGAACGAGGGTTTGTTGGCAGATACTAGCGCGAGCGGCAGCGACGCCGAGTTTGGGTTTTCGAGGGGCGAGTTCCGGACGAGTCAACTCGCCGGCACGGTGGAGTTTTATCAGAGGCATGTGTTTTTGTGCTACAAGAACCCGCAGGTGTGGCCGCCGAGGATTGAGGCGGCGGAGTTTGATCGGCTGCCGAGGCTGCTCCACGCGGCGGTGATGGCGAGGAGGGTGGATATGAGGAAAGAGATTTGGTGGAAACATTGCAGGAGATTGACACATTTTGATGTTGACACATTCGTTGAAGAAGTTCTTGTGAAGGATGGTGAGTGGCTGCCTGGAACTCCTGAAACTCTGAGGGGTTCATACATATTTGTATGTTCTCATGGGTCCAGGGATCGCCGTTGTGGAGTCTGTGGGCCTCCCTTGATCAATAGATTCAGAGAAGAGATAGATTTGCATGGTCTACAAGGTAAAGTGTCTGTCAGGCCATGTTCACACATTGGGGGGCATAAGTATGCAGGAAATGTAATTATATTTGGAACAAATATCAACAAAGAAGTCACTGGCCACTGGTATGGATATGTTGCTCCAGAAGATGTACCTCAATTGCTTGAGCAGCATATTGGGAGAGGAGAAATTGTAGACTGGCTATGGAGGGGTCAAATGGGTTTATCAGAAGAACAACAGATGAAATCTCAAGAACTAAGGCTCCAGCCCAATGGTGAGTCAAATGTGGAGAAAAGAAGTACAGAGCTGACACAAACGAAGGAAAGGGGGATGAATACTGGTACATGCAGATCTCAAGTTGAAATTGGGGGCTGTTGCCAGGAAAACGGAAGCTCTTCTTGCTGCCAGAACACTTCGTTAACAGATAGGATAAGTAGTCCTGATTTGAATATGATGGCAACACAAGCGACATCTGAAAAGAAAAAGAGCAGTAGCAAACTACTTTCACGCAGAAGCAAAGCTGCATCTGCTAGAAAGTTTTGTGCTATGCCAACATGGTCTGAGAGGTGGGAGCGGGAAGATACATATGCAGCTTTGGCTATTGTTTGTGCTGCTGTGTCGGTTGGTGTTGCTTACAGTTGCTACAAACAGTTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

417

Amino Acids

46.55

Weight (kDa)

7.13

Isoelectric Point (pI)

51.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 80 - 269 2.2e-37 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 286
AccBSI CCGCTC 3 cut(s) 91, 186, 1168
AccI GTMKAC 2 cut(s) 610, 789
AccII CGCG 2 cut(s) 182, 356
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 9 cut(s) 89, 186, 293, 305, 320, 323, 356, 359, 1168
AclWI GGATC 1 cut(s) 549
AcoI YGGCCR 2 cut(s) 302, 712
AcsI RAATTY 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 47, 195
AfaI GTAC 3 cut(s) 747, 895, 937
AfiI CCNNNNNNNGG 5 cut(s) 95, 126, 157, 204, 358
AgsI TTSAA 3 cut(s) 449, 956, 1042
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 3 cut(s) 481, 536, 972
AloI GAACNNNNNNTCC 2 cut(s) 840, 872
AluBI AGCT 5 cut(s) 147, 901, 987, 1115, 1187
AluI AGCT 5 cut(s) 147, 901, 987, 1115, 1187
Alw26I GTCTC 2 cut(s) 9, 15
AlwI GGATC 1 cut(s) 549
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 4 cut(s) 302, 563, 632, 712
ApeKI GCWGC 9 cut(s) 189, 337, 346, 478, 763, 996, 1115, 1184, 1205
ApoI RAATTY 1 cut(s) 115
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
Asp700I GAANNNNTTC 2 cut(s) 453, 988
AspLEI GCGC 3 cut(s) 11, 137, 182
AspS9I GGNCC 3 cut(s) 129, 534, 563
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 3 cut(s) 373, 482, 881
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 2 cut(s) 129, 534
BalI TGGCCA 1 cut(s) 714
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 9 cut(s) 201, 324, 333, 465, 775, 983, 1102, 1192, 1196
BccI CCATC 4 cut(s) 61, 358, 461, 1042
BceAI ACGGC 1 cut(s) 531
BciT130I CCWGG 3 cut(s) 483, 538, 974
BclI TGATCA 1 cut(s) 573
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 9, 15
BfaI CTAG 3 cut(s) 6, 177, 1125
BfoI RGCGCY 2 cut(s) 12, 138
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 944
Bme1390I CCNGG 4 cut(s) 142, 483, 538, 974
Bme18I GGWCC 2 cut(s) 129, 534
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 60
BmgT120I GGNCC 3 cut(s) 129, 534, 563
BmiI GGNNCC 4 cut(s) 101, 130, 535, 857
BmrFI CCNGG 4 cut(s) 142, 483, 538, 974
BmsI GCATC 1 cut(s) 1127
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 2 cut(s) 720, 842
BpuEI CTTGAG 3 cut(s) 779, 828, 933
BpuMI CCSGG 1 cut(s) 142
BsaHI GRCGYC 2 cut(s) 47, 195
BsaI GGTCTC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 308, 341, 537
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 771, 801, 840, 870
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 126, 157, 204, 358
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 4 cut(s) 715, 722, 797, 937
Bse3DI GCAATG 1 cut(s) 406
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 3 cut(s) 483, 538, 974
BseDI CCNNGG 3 cut(s) 308, 341, 537
BseGI GGATG 3 cut(s) 53, 472, 930
BseLI CCNNNNNNNGG 5 cut(s) 95, 126, 157, 204, 358
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 491, 1146
BseNI ACTGG 4 cut(s) 715, 722, 797, 937
BseRI GAGGAG 2 cut(s) 385, 792
BseXI GCAGC 9 cut(s) 201, 324, 333, 465, 775, 983, 1102, 1192, 1196
Bsh1236I CGCG 2 cut(s) 182, 356
BshFI GGCC 4 cut(s) 304, 565, 634, 714
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 3 cut(s) 141, 226, 243
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 5 cut(s) 95, 126, 157, 204, 358
BsmAI GTCTC 2 cut(s) 9, 15
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 4 cut(s) 304, 565, 634, 714
Bso31I GGTCTC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 12
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 4 cut(s) 331, 541, 573, 944
BspACI CCGC 9 cut(s) 89, 186, 293, 305, 320, 323, 356, 359, 1168
BspANI GGCC 4 cut(s) 304, 565, 634, 714
BspCNI CTCAG 2 cut(s) 492, 1147
BspEI TCCGGA 1 cut(s) 225
BspFNI CGCG 2 cut(s) 182, 356
BspLI GGNNCC 4 cut(s) 101, 130, 535, 857
BspMI ACCTGC 1 cut(s) 286
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 549
BspQI GCTCTTC 1 cut(s) 994
BspTNI GGTCTC 1 cut(s) 9
BsrBI CCGCTC 3 cut(s) 91, 186, 1168
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 4 cut(s) 715, 722, 797, 937
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 3 cut(s) 308, 341, 537
BssMI GATC 4 cut(s) 331, 541, 573, 944
BssNI GRCGYC 2 cut(s) 47, 195
Bst2UI CCWGG 3 cut(s) 483, 538, 974
Bst4CI ACNGT 3 cut(s) 250, 1232, 1245
Bst6I CTCTTC 2 cut(s) 585, 994
BstACI GRCGYC 2 cut(s) 47, 195
BstC8I GCNNGC 3 cut(s) 7, 184, 244
BstDEI CTNAG 3 cut(s) 500, 851, 1155
BstF5I GGATG 3 cut(s) 53, 472, 930
BstFNI CGCG 2 cut(s) 182, 356
BstH2I RGCGCY 2 cut(s) 12, 138
BstHHI GCGC 3 cut(s) 11, 137, 182
BstKTI GATC 4 cut(s) 334, 544, 576, 947
BstMAI GTCTC 2 cut(s) 9, 15
BstMBI GATC 4 cut(s) 331, 541, 573, 944
BstNI CCWGG 3 cut(s) 483, 538, 974
BstNSI RCATGY 2 cut(s) 272, 942
BstSCI CCNGG 4 cut(s) 140, 481, 536, 972
BstUI CGCG 2 cut(s) 182, 356
BstV1I GCAGC 9 cut(s) 201, 324, 333, 465, 775, 983, 1102, 1192, 1196
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 944
BstXI CCANNNNNNTGG 2 cut(s) 722, 866
BstYI RGATCY 1 cut(s) 944
BsuRI GGCC 4 cut(s) 304, 565, 634, 714
BtrI CACGTC 1 cut(s) 60
BtsCI GGATG 3 cut(s) 53, 472, 930
BtsIMutI CAGTG 2 cut(s) 708, 715
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 7, 184, 244
CfoI GCGC 3 cut(s) 11, 137, 182
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 3 cut(s) 129, 534, 563
CseI GACGC 2 cut(s) 55, 203
Csp6I GTAC 3 cut(s) 746, 894, 936
CviAII CATG 6 cut(s) 269, 530, 605, 636, 939, 1149
CviQI GTAC 3 cut(s) 746, 894, 936
DdeI CTNAG 3 cut(s) 500, 851, 1155
DpnI GATC 4 cut(s) 333, 543, 575, 946
DpnII GATC 4 cut(s) 331, 541, 573, 944
EaeI YGGCCR 2 cut(s) 302, 712
Eam1104I CTCTTC 2 cut(s) 585, 994
EarI CTCTTC 2 cut(s) 585, 994
EciI GGCGGA 1 cut(s) 338
Eco31I GGTCTC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 129, 534
Eco88I CYCGRG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 3 cut(s) 481, 536, 972
EcoRV GATATC 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 6 cut(s) 272, 533, 608, 639, 942, 1152
FalI AAGNNNNNCTT 4 cut(s) 441, 473, 976, 1008
FaqI GGGAC 1 cut(s) 142
FatI CATG 6 cut(s) 268, 529, 604, 635, 938, 1148
FauI CCCGC 3 cut(s) 96, 300, 1161
FauNDI CATATG 1 cut(s) 1180
FbaI TGATCA 1 cut(s) 573
FblI GTMKAC 2 cut(s) 610, 789
FokI GGATG 3 cut(s) 40, 479, 937
FspBI CTAG 3 cut(s) 6, 177, 1125
GlaI GCGC 3 cut(s) 10, 136, 181
GsuI CTGGAG 2 cut(s) 720, 842
HaeII RGCGCY 2 cut(s) 12, 138
HaeIII GGCC 4 cut(s) 304, 565, 634, 714
HapII CCGG 3 cut(s) 141, 226, 243
HgaI GACGC 2 cut(s) 55, 203
HhaI GCGC 3 cut(s) 11, 137, 182
Hin1I GRCGYC 2 cut(s) 47, 195
Hin1II CATG 6 cut(s) 272, 533, 608, 639, 942, 1152
Hin6I GCGC 3 cut(s) 9, 135, 180
HinP1I GCGC 3 cut(s) 9, 135, 180
HincII GTYRAC 3 cut(s) 236, 436, 1014
HindII GTYRAC 3 cut(s) 236, 436, 1014
HinfI GANTC 5 cut(s) 26, 232, 555, 582, 872
HpaI GTTAAC 1 cut(s) 1014
HpaII CCGG 3 cut(s) 141, 226, 243
HphI GGTGA 3 cut(s) 373, 482, 881
Hpy166II GTNNAC 6 cut(s) 236, 436, 611, 641, 790, 1014
Hpy188I TCNGA 8 cut(s) 85, 109, 264, 501, 587, 823, 1069, 1156
Hpy188III TCNNGA 7 cut(s) 127, 151, 226, 491, 737, 845, 1034
Hpy8I GTNNAC 6 cut(s) 236, 436, 611, 641, 790, 1014
Hpy99I CGWCG 4 cut(s) 49, 64, 98, 197
HpyAV CCTTC 2 cut(s) 457, 907
HpyCH4III ACNGT 3 cut(s) 250, 1232, 1245
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 6 cut(s) 411, 604, 665, 942, 1118, 1184
HpyF3I CTNAG 3 cut(s) 500, 851, 1155
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 2 cut(s) 47, 195
Hsp92II CATG 6 cut(s) 272, 533, 608, 639, 942, 1152
HspAI GCGC 3 cut(s) 9, 135, 180
Kpn2I TCCGGA 1 cut(s) 225
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 573
KspAI GTTAAC 1 cut(s) 1014
Kzo9I GATC 4 cut(s) 331, 541, 573, 944
LguI GCTCTTC 1 cut(s) 994
LmnI GCTCC 6 cut(s) 39, 144, 354, 739, 861, 1165
Lsp1109I GCAGC 9 cut(s) 201, 324, 333, 465, 775, 983, 1102, 1192, 1196
LweI GCATC 1 cut(s) 1127
MaeI CTAG 3 cut(s) 6, 177, 1125
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 706
MalI GATC 4 cut(s) 333, 543, 575, 946
MbiI CCGCTC 3 cut(s) 91, 186, 1168
MboI GATC 4 cut(s) 331, 541, 573, 944
MboII GAAGA 7 cut(s) 57, 461, 602, 752, 836, 981, 1184
MfeI CAATTG 1 cut(s) 752
MflI RGATCY 1 cut(s) 944
MlsI TGGCCA 1 cut(s) 714
MluCI AATT 5 cut(s) 115, 675, 752, 783, 957
MluNI TGGCCA 1 cut(s) 714
MlyI GAGTC 4 cut(s) 20, 241, 564, 881
MmeI TCCRAC 2 cut(s) 108, 132
Mox20I TGGCCA 1 cut(s) 714
MroI TCCGGA 1 cut(s) 225
MroNI GCCGGC 1 cut(s) 242
MroXI GAANNNNTTC 2 cut(s) 453, 988
MscI TGGCCA 1 cut(s) 714
MseI TTAA 1 cut(s) 1013
MslI CAYNNNNRTG 3 cut(s) 429, 660, 720
Msp20I TGGCCA 1 cut(s) 714
MspI CCGG 3 cut(s) 141, 226, 243
MspR9I CCNGG 4 cut(s) 142, 483, 538, 974
MunI CAATTG 1 cut(s) 752
MvaI CCWGG 3 cut(s) 483, 538, 974
MvnI CGCG 2 cut(s) 182, 356
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 1 cut(s) 142
NdeI CATATG 1 cut(s) 1180
NdeII GATC 4 cut(s) 331, 541, 573, 944
NgoMIV GCCGGC 1 cut(s) 242
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 6 cut(s) 272, 533, 608, 639, 942, 1152
NlaIV GGNNCC 4 cut(s) 101, 130, 535, 857
NmeAIII GCCGAG 3 cut(s) 223, 333, 366
NmuCI GTSAC 1 cut(s) 706
NspI RCATGY 2 cut(s) 272, 942
PaqCI CACCTGC 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 994
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PdiI GCCGGC 1 cut(s) 244
PdmI GAANNNNTTC 2 cut(s) 453, 988
PfeI GAWTC 1 cut(s) 582
PfoI TCCNGGA 1 cut(s) 140
PleI GAGTC 4 cut(s) 20, 240, 563, 880
PpsI GAGTC 4 cut(s) 20, 240, 563, 880
Psp6I CCWGG 3 cut(s) 481, 536, 972
PspGI CCWGG 3 cut(s) 481, 536, 972
PspN4I GGNNCC 4 cut(s) 101, 130, 535, 857
PspPI GGNCC 3 cut(s) 129, 534, 563
PsuI RGATCY 1 cut(s) 944
RsaI GTAC 3 cut(s) 747, 895, 937
RsaNI GTAC 3 cut(s) 746, 894, 936
RseI CAYNNNNRTG 3 cut(s) 429, 660, 720
SapI GCTCTTC 1 cut(s) 994
SaqAI TTAA 1 cut(s) 1013
Sau3AI GATC 4 cut(s) 331, 541, 573, 944
Sau96I GGNCC 3 cut(s) 129, 534, 563
SchI GAGTC 4 cut(s) 20, 241, 564, 881
ScrFI CCNGG 4 cut(s) 142, 483, 538, 974
SfaNI GCATC 1 cut(s) 1127
SinI GGWCC 2 cut(s) 129, 534
SmiMI CAYNNNNRTG 3 cut(s) 429, 660, 720
SmlI CTYRAG 3 cut(s) 758, 843, 948
SmoI CTYRAG 3 cut(s) 758, 843, 948
Sse9I AATT 5 cut(s) 115, 675, 752, 783, 957
SsiI CCGC 9 cut(s) 89, 186, 293, 305, 320, 323, 356, 359, 1168
SspMI CTAG 3 cut(s) 6, 177, 1125
StyD4I CCNGG 4 cut(s) 140, 481, 536, 972
TaaI ACNGT 3 cut(s) 250, 1232, 1245
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 93, 212
TaqII GACCGA 1 cut(s) 33
TasI AATT 5 cut(s) 115, 675, 752, 783, 957
TatI WGTACW 1 cut(s) 893
TauI GCSGC 4 cut(s) 189, 307, 323, 359
TfiI GAWTC 1 cut(s) 582
Tru1I TTAA 1 cut(s) 1013
Tru9I TTAA 1 cut(s) 1013
TscAI CASTG 2 cut(s) 715, 722
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 9 cut(s) 189, 337, 346, 478, 763, 996, 1115, 1184, 1205
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 3 cut(s) 498, 851, 941
TspGWI ACGGA 1 cut(s) 996
TspRI CASTG 2 cut(s) 715, 722
VpaK11BI GGWCC 2 cut(s) 129, 534
XapI RAATTY 1 cut(s) 115
XceI RCATGY 2 cut(s) 272, 942
XmiI GTMKAC 2 cut(s) 610, 789
XmnI GAANNNNTTC 2 cut(s) 453, 988
XspI CTAG 3 cut(s) 6, 177, 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.