Rh5BG524800

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007599
Physical Location & Seq
Reverse (-)
82775389 .. 82779029
3641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG524800.1

Sequence Viewer

Length: 1320 bp
ATGGCTAGCGCCCGAGACCGAGACGACTCGTTGCTCCCATTTACGACGCCCCCATCCACGTCGTCTTCCCCGATTGTGATATCCGACCCGCTCGACGGGTTCCTCTCCGACCCGAATTCCCACATCGGGACCAGCGCCTCCGGGAGCTTCCAGAACGAGGGTTTGTTGGCAGATACTAGCGCGAGCGGCAGCGACGCCGAGTTTGGGTTTTCGAGGGGCGAGTTCCGGACGAGTCAACTCGCCGGCACGGTGGAGTTTTATCAGAGGCATGTGTTTTTGTGCTACAAGAACCCGCAGGTGTGGCCGCCGAGGATTGAGGCGGCGGAGTTTGATCGGCTGCCGAGGCTGCTCCACGCGGCCGTGATGGCGAGGAGGGTGGATATGAGGAAACAGACTCGCTTAACAATATGTGAAGGGCACGATGGAACTGAGACTTCAAATGGTGATGTATTAATTTTCCCCGACATGATCAGATACAGGAGATTGACACATTTTGATGTTGATACATTCGTTGAAGAAGTTCTTGTGAAGGATGGTGAGTGGCTGCCTGGAACTCCTGAAACTCTGAGGGGTTCATACATATTTGTATGTTCTCATGGGTCCAGGGATCGCCGTTGTGGAGTCTGTGGGCCTCCCTTGATCAATAGATTCAGAGAAGAGATAGATTTGCATGGTCTACAAGGTAAAGTGTCTGTTAGGCCATGTTCACACATTGGGGGGCATAAGTATGCAGGAAATGTAATTATATTTGGATCAAATATCAACAAAGAAGTCACTGGCCACTGGTATGGATATGTTGCTCCAGAAGATGTACCTCAATTGCTTGAGCAGCATATTGGGAGAGGAGAAATTGTAGACTGGCTATGGAGGGGTCAAATGGGTTTATCAGAAGAACAACAGAAGAAATCTCAAGAACTAAGGCTCCAGCCCAATGGTGAGTCAAATGTGGAGAAAAGAAGTACAGAGCTGACACAAACGAAGGAAAGGGGGATGGATACTGGTACATGCAGATCTCAAGTTGAAATTGGGGGCTGTTGCCAGGAAAACGGAAGCTCTTCTTGCTGCCAGAACACTTCGTTAACAGATAGGATAAGTAGTCCTGATTTGAATATGATGGCAACACAAGCGACATCTGAAAAGAAAAAGAGCAGTAGCAAACTACTTTCACGCAGAAGCAAAGCTGCATCTGCTCGAAAGTTTTGTGCTATGCCAACATGGTCTGAGAGCTGGGAGCGGGAAGATACATATGCAGCTTTGGCTATCGTTTGTGCTGCTGTGTCGGTTGGTGTTGCTTACAGTTGCTACAAACAGTTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

439

Amino Acids

48.75

Weight (kDa)

6.29

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 80 - 291 4.1e-46 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
Acc36I ACCTGC 1 cut(s) 286
AccBSI CCGCTC 3 cut(s) 91, 186, 1234
AccI GTMKAC 2 cut(s) 676, 855
AccII CGCG 2 cut(s) 182, 356
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 8 cut(s) 89, 186, 293, 305, 320, 323, 356, 1234
AclWI GGATC 2 cut(s) 615, 760
AcoI YGGCCR 3 cut(s) 302, 357, 778
AcsI RAATTY 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 47, 195
AfaI GTAC 3 cut(s) 813, 961, 1003
AfiI CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
AgsI TTSAA 4 cut(s) 438, 515, 1022, 1108
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 3 cut(s) 547, 602, 1038
AloI GAACNNNNNNTCC 2 cut(s) 906, 938
AluBI AGCT 6 cut(s) 147, 967, 1053, 1181, 1227, 1253
AluI AGCT 6 cut(s) 147, 967, 1053, 1181, 1227, 1253
Alw26I GTCTC 3 cut(s) 9, 15, 425
AlwI GGATC 2 cut(s) 615, 760
Ama87I CYCGRG 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 5 cut(s) 302, 357, 629, 698, 778
ApeKI GCWGC 9 cut(s) 189, 337, 346, 544, 829, 1062, 1181, 1250, 1271
ApoI RAATTY 1 cut(s) 115
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AseI ATTAAT 1 cut(s) 452
Asp700I GAANNNNTTC 2 cut(s) 519, 1054
AspLEI GCGC 3 cut(s) 11, 137, 182
AspS9I GGNCC 3 cut(s) 129, 600, 629
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 3 cut(s) 455, 548, 947
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 2 cut(s) 129, 600
BaeGI GKGCMC 1 cut(s) 420
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BalI TGGCCA 1 cut(s) 780
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 9 cut(s) 201, 324, 333, 531, 841, 1049, 1168, 1258, 1262
BccI CCATC 6 cut(s) 61, 358, 416, 527, 985, 1108
BceAI ACGGC 2 cut(s) 344, 597
BciT130I CCWGG 3 cut(s) 549, 604, 1040
BciVI GTATCC 1 cut(s) 988
BclI TGATCA 2 cut(s) 468, 639
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 3 cut(s) 9, 15, 425
BfaI CTAG 2 cut(s) 6, 177
BfoI RGCGCY 2 cut(s) 12, 138
BfuAI ACCTGC 1 cut(s) 286
BfuI GTATCC 1 cut(s) 988
BglI GCCNNNNNGGC 1 cut(s) 365
BglII AGATCT 1 cut(s) 1010
Bme1390I CCNGG 4 cut(s) 142, 549, 604, 1040
Bme18I GGWCC 2 cut(s) 129, 600
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 60
BmgT120I GGNCC 3 cut(s) 129, 600, 629
BmiI GGNNCC 4 cut(s) 101, 130, 601, 923
BmrFI CCNGG 4 cut(s) 142, 549, 604, 1040
BmsI GCATC 1 cut(s) 1193
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 2 cut(s) 786, 908
BpuEI CTTGAG 3 cut(s) 845, 894, 999
BpuMI CCSGG 1 cut(s) 142
BsaHI GRCGYC 2 cut(s) 47, 195
BsaI GGTCTC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 308, 341, 603
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 837, 867, 906, 936
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 4 cut(s) 781, 788, 863, 1003
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 3 cut(s) 549, 604, 1040
BseDI CCNNGG 3 cut(s) 308, 341, 603
BseGI GGATG 3 cut(s) 53, 538, 996
BseLI CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
BseMII CTCAG 3 cut(s) 420, 557, 1212
BseNI ACTGG 4 cut(s) 781, 788, 863, 1003
BseRI GAGGAG 2 cut(s) 385, 858
BseSI GKGCMC 1 cut(s) 420
BseX3I CGGCCG 1 cut(s) 357
BseXI GCAGC 9 cut(s) 201, 324, 333, 531, 841, 1049, 1168, 1258, 1262
BseYI CCCAGC 1 cut(s) 1227
Bsh1236I CGCG 2 cut(s) 182, 356
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 5 cut(s) 304, 359, 631, 700, 780
BsiEI CGRYCG 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 3 cut(s) 141, 226, 243
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 4 cut(s) 95, 126, 157, 204
BsmAI GTCTC 3 cut(s) 9, 15, 425
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 5 cut(s) 304, 359, 631, 700, 780
Bso31I GGTCTC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 420
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
BspACI CCGC 8 cut(s) 89, 186, 293, 305, 320, 323, 356, 1234
BspANI GGCC 5 cut(s) 304, 359, 631, 700, 780
BspCNI CTCAG 3 cut(s) 421, 558, 1213
BspEI TCCGGA 1 cut(s) 225
BspFNI CGCG 2 cut(s) 182, 356
BspLI GGNNCC 4 cut(s) 101, 130, 601, 923
BspMI ACCTGC 1 cut(s) 286
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 615, 760
BspQI GCTCTTC 1 cut(s) 1060
BspTNI GGTCTC 1 cut(s) 9
BsrBI CCGCTC 3 cut(s) 91, 186, 1234
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 4 cut(s) 781, 788, 863, 1003
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 3 cut(s) 308, 341, 603
BssMI GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
BssNI GRCGYC 2 cut(s) 47, 195
Bst2UI CCWGG 3 cut(s) 549, 604, 1040
Bst4CI ACNGT 3 cut(s) 250, 1298, 1311
Bst6I CTCTTC 2 cut(s) 651, 1060
BstACI GRCGYC 2 cut(s) 47, 195
BstC8I GCNNGC 3 cut(s) 7, 184, 244
BstDEI CTNAG 4 cut(s) 429, 566, 917, 1221
BstF5I GGATG 3 cut(s) 53, 538, 996
BstFNI CGCG 2 cut(s) 182, 356
BstH2I RGCGCY 2 cut(s) 12, 138
BstHHI GCGC 3 cut(s) 11, 137, 182
BstKTI GATC 6 cut(s) 334, 471, 610, 642, 755, 1013
BstMAI GTCTC 3 cut(s) 9, 15, 425
BstMBI GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
BstMCI CGRYCG 1 cut(s) 360
BstNI CCWGG 3 cut(s) 549, 604, 1040
BstNSI RCATGY 2 cut(s) 272, 1008
BstSCI CCNGG 4 cut(s) 140, 547, 602, 1038
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 2 cut(s) 182, 356
BstV1I GCAGC 9 cut(s) 201, 324, 333, 531, 841, 1049, 1168, 1258, 1262
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 1010
BstXI CCANNNNNNTGG 2 cut(s) 788, 932
BstYI RGATCY 1 cut(s) 1010
BstZI CGGCCG 1 cut(s) 357
BsuI GTATCC 1 cut(s) 988
BsuRI GGCC 5 cut(s) 304, 359, 631, 700, 780
BtrI CACGTC 1 cut(s) 60
BtsCI GGATG 3 cut(s) 53, 538, 996
BtsIMutI CAGTG 2 cut(s) 774, 781
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 7, 184, 244
CfoI GCGC 3 cut(s) 11, 137, 182
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 3 cut(s) 129, 600, 629
CseI GACGC 2 cut(s) 55, 203
Csp6I GTAC 3 cut(s) 812, 960, 1002
CviAII CATG 7 cut(s) 269, 466, 596, 671, 702, 1005, 1215
CviQI GTAC 3 cut(s) 812, 960, 1002
DdeI CTNAG 4 cut(s) 429, 566, 917, 1221
DpnI GATC 6 cut(s) 333, 470, 609, 641, 754, 1012
DpnII GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
EaeI YGGCCR 3 cut(s) 302, 357, 778
EagI CGGCCG 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 651, 1060
EarI CTCTTC 2 cut(s) 651, 1060
EciI GGCGGA 1 cut(s) 338
EclXI CGGCCG 1 cut(s) 357
Eco31I GGTCTC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 129, 600
Eco52I CGGCCG 1 cut(s) 357
Eco88I CYCGRG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 3 cut(s) 547, 602, 1038
EcoRV GATATC 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 7 cut(s) 272, 469, 599, 674, 705, 1008, 1218
FalI AAGNNNNNCTT 4 cut(s) 507, 539, 1042, 1074
FaqI GGGAC 1 cut(s) 142
FatI CATG 7 cut(s) 268, 465, 595, 670, 701, 1004, 1214
FauI CCCGC 3 cut(s) 96, 300, 1227
FauNDI CATATG 1 cut(s) 1246
FbaI TGATCA 2 cut(s) 468, 639
FblI GTMKAC 2 cut(s) 676, 855
FokI GGATG 3 cut(s) 40, 545, 1003
FspBI CTAG 2 cut(s) 6, 177
GlaI GCGC 3 cut(s) 10, 136, 181
GsaI CCCAGC 1 cut(s) 1231
GsuI CTGGAG 2 cut(s) 786, 908
HaeII RGCGCY 2 cut(s) 12, 138
HaeIII GGCC 5 cut(s) 304, 359, 631, 700, 780
HapII CCGG 3 cut(s) 141, 226, 243
HgaI GACGC 2 cut(s) 55, 203
HhaI GCGC 3 cut(s) 11, 137, 182
Hin1I GRCGYC 2 cut(s) 47, 195
Hin1II CATG 7 cut(s) 272, 469, 599, 674, 705, 1008, 1218
Hin6I GCGC 3 cut(s) 9, 135, 180
HinP1I GCGC 3 cut(s) 9, 135, 180
HincII GTYRAC 2 cut(s) 236, 1080
HindII GTYRAC 2 cut(s) 236, 1080
HinfI GANTC 6 cut(s) 26, 232, 394, 621, 648, 938
HpaI GTTAAC 1 cut(s) 1080
HpaII CCGG 3 cut(s) 141, 226, 243
HphI GGTGA 3 cut(s) 455, 548, 947
Hpy166II GTNNAC 5 cut(s) 236, 677, 707, 856, 1080
Hpy188I TCNGA 9 cut(s) 85, 109, 264, 473, 567, 653, 889, 1135, 1222
Hpy188III TCNNGA 7 cut(s) 127, 151, 226, 557, 803, 911, 1100
Hpy8I GTNNAC 5 cut(s) 236, 677, 707, 856, 1080
Hpy99I CGWCG 4 cut(s) 49, 64, 98, 197
HpyAV CCTTC 3 cut(s) 407, 523, 973
HpyCH4III ACNGT 3 cut(s) 250, 1298, 1311
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 5 cut(s) 670, 731, 1008, 1184, 1250
HpyF3I CTNAG 4 cut(s) 429, 566, 917, 1221
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 2 cut(s) 47, 195
Hsp92II CATG 7 cut(s) 272, 469, 599, 674, 705, 1008, 1218
HspAI GCGC 3 cut(s) 9, 135, 180
Kpn2I TCCGGA 1 cut(s) 225
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Ksp22I TGATCA 2 cut(s) 468, 639
KspAI GTTAAC 1 cut(s) 1080
Kzo9I GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
LguI GCTCTTC 1 cut(s) 1060
LmnI GCTCC 6 cut(s) 39, 144, 354, 805, 927, 1231
Lsp1109I GCAGC 9 cut(s) 201, 324, 333, 531, 841, 1049, 1168, 1258, 1262
LweI GCATC 1 cut(s) 1193
MaeI CTAG 2 cut(s) 6, 177
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 772
MalI GATC 6 cut(s) 333, 470, 609, 641, 754, 1012
MbiI CCGCTC 3 cut(s) 91, 186, 1234
MboI GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
MboII GAAGA 8 cut(s) 57, 527, 668, 818, 902, 913, 1047, 1250
MfeI CAATTG 1 cut(s) 818
MflI RGATCY 1 cut(s) 1010
MhlI GDGCHC 1 cut(s) 420
MlsI TGGCCA 1 cut(s) 780
MluCI AATT 6 cut(s) 115, 453, 741, 818, 849, 1023
MluNI TGGCCA 1 cut(s) 780
MlyI GAGTC 5 cut(s) 20, 241, 388, 630, 947
MmeI TCCRAC 2 cut(s) 108, 132
Mox20I TGGCCA 1 cut(s) 780
MroI TCCGGA 1 cut(s) 225
MroNI GCCGGC 1 cut(s) 242
MroXI GAANNNNTTC 2 cut(s) 519, 1054
MscI TGGCCA 1 cut(s) 780
MseI TTAA 3 cut(s) 401, 452, 1079
MslI CAYNNNNRTG 3 cut(s) 495, 726, 786
Msp20I TGGCCA 1 cut(s) 780
MspI CCGG 3 cut(s) 141, 226, 243
MspR9I CCNGG 4 cut(s) 142, 549, 604, 1040
MunI CAATTG 1 cut(s) 818
MvaI CCWGG 3 cut(s) 549, 604, 1040
MvnI CGCG 2 cut(s) 182, 356
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 1 cut(s) 142
NdeI CATATG 1 cut(s) 1246
NdeII GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
NgoMIV GCCGGC 1 cut(s) 242
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 7 cut(s) 272, 469, 599, 674, 705, 1008, 1218
NlaIV GGNNCC 4 cut(s) 101, 130, 601, 923
NmeAIII GCCGAG 3 cut(s) 223, 333, 366
NmuCI GTSAC 1 cut(s) 772
NspI RCATGY 2 cut(s) 272, 1008
PaqCI CACCTGC 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 1060
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PdiI GCCGGC 1 cut(s) 244
PdmI GAANNNNTTC 2 cut(s) 519, 1054
PfeI GAWTC 1 cut(s) 648
PfoI TCCNGGA 1 cut(s) 140
PleI GAGTC 5 cut(s) 20, 240, 388, 629, 946
PpsI GAGTC 5 cut(s) 20, 240, 388, 629, 946
PshBI ATTAAT 1 cut(s) 452
Psp6I CCWGG 3 cut(s) 547, 602, 1038
PspFI CCCAGC 1 cut(s) 1227
PspGI CCWGG 3 cut(s) 547, 602, 1038
PspN4I GGNNCC 4 cut(s) 101, 130, 601, 923
PspPI GGNCC 3 cut(s) 129, 600, 629
PsuI RGATCY 1 cut(s) 1010
RsaI GTAC 3 cut(s) 813, 961, 1003
RsaNI GTAC 3 cut(s) 812, 960, 1002
RseI CAYNNNNRTG 3 cut(s) 495, 726, 786
SapI GCTCTTC 1 cut(s) 1060
SaqAI TTAA 3 cut(s) 401, 452, 1079
Sau3AI GATC 6 cut(s) 331, 468, 607, 639, 752, 1010
Sau96I GGNCC 3 cut(s) 129, 600, 629
SchI GAGTC 5 cut(s) 20, 241, 388, 630, 947
ScrFI CCNGG 4 cut(s) 142, 549, 604, 1040
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 1 cut(s) 1193
SinI GGWCC 2 cut(s) 129, 600
SmiMI CAYNNNNRTG 3 cut(s) 495, 726, 786
SmlI CTYRAG 3 cut(s) 824, 909, 1014
SmoI CTYRAG 3 cut(s) 824, 909, 1014
Sse9I AATT 6 cut(s) 115, 453, 741, 818, 849, 1023
SsiI CCGC 8 cut(s) 89, 186, 293, 305, 320, 323, 356, 1234
SspMI CTAG 2 cut(s) 6, 177
StyD4I CCNGG 4 cut(s) 140, 547, 602, 1038
TaaI ACNGT 3 cut(s) 250, 1298, 1311
TaiI ACGT 1 cut(s) 62
TaqI TCGA 3 cut(s) 93, 212, 1192
TaqII GACCGA 1 cut(s) 33
TasI AATT 6 cut(s) 115, 453, 741, 818, 849, 1023
TatI WGTACW 1 cut(s) 959
TauI GCSGC 4 cut(s) 189, 307, 323, 359
TfiI GAWTC 1 cut(s) 648
Tru1I TTAA 3 cut(s) 401, 452, 1079
Tru9I TTAA 3 cut(s) 401, 452, 1079
TscAI CASTG 2 cut(s) 781, 788
TseFI GTSAC 1 cut(s) 772
TseI GCWGC 9 cut(s) 189, 337, 346, 544, 829, 1062, 1181, 1250, 1271
Tsp45I GTSAC 1 cut(s) 772
TspDTI ATGAA 1 cut(s) 564
TspGWI ACGGA 1 cut(s) 1062
TspRI CASTG 2 cut(s) 781, 788
VpaK11BI GGWCC 2 cut(s) 129, 600
VspI ATTAAT 1 cut(s) 452
XapI RAATTY 1 cut(s) 115
XceI RCATGY 2 cut(s) 272, 1008
XmiI GTMKAC 2 cut(s) 676, 855
XmnI GAANNNNTTC 2 cut(s) 519, 1054
XspI CTAG 2 cut(s) 6, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.