MD11G1032600.v1.1

Sucrase/ferredoxin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007599
Physical Location & Seq
Forward (+)
2830720 .. 2832052
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1032600.v1.1.491

Sequence Viewer

Length: 840 bp
ATGAAATGCAGGAGATTGACACATTTTGATGTTGATACATTCGTTGAAGAAGTTCTTGTGAAGGATGGTGAATGGCAACCTGGAACTCCTGAAACTCTGACGGGTTCGTATGTTTTTGTATGTTCTCATGGGTCCCGGGATCGCCGTTGTGGAGTCTGTGGTCCTCCATTGGTCAGTAGATTCAGAGAAGAGATAGAATTACATGGTCTTCAAGGTAAAGTGTCCGTTAGTCCATGTTCACACATTGGGGGGCATAAGTATGCTGGAAATGTTATCATATATGGATCAAATTGCAACAGAGAAGTCTCTGGCCACTGGTATGGATATGTTGCTCCAGAAGACATAACTGTATTGCTTGAACAGCATGTCGGGAAAGGAGAAATTGTAGACTGGCTATGGAGGGGTCAGATGGGTTTATCGGAAGAACAGCAGAAGGAATCTCAAGAACAAAGGCTCCAGCTTAACGGCGAGAGAGATGTGGGAAAAACCACTACAGAGTTGACACAACCAAAGGATAGGGGGAATGCTAGTGTATGTAGATCTCAAGTTGAGAACGGGGGCTTTTGCCAGGGAAATGGAAAGTCTTGCTGTCAGAATGCTATGATAGATAGTCCCGACTTGAATGAGATTGCACCGAAGGCAACAGGTGAAAAGAAAAAAAGCAGCAGGAAACTACTTTCACGAAAGAACAGTGGCAAAGGGGCATCCACGCGCAAGGTTTGTGCAATGCCAACATGGTTTCAGAGCTGGGAGCGTGAAGATACGCATGCAGCTTTAGCTGTTGTTTGTGCCGCAGTGTCAGTTGCCGTTGCTTACAAGTGCTACAAACAGTTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Suc_Fer-like

Protein Analysis

280

Amino Acids

30.86

Weight (kDa)

8.18

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Suc_Fer-like PF06999 6 - 135 8.9e-35 Sucrase/ferredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 387
AccII CGCG 1 cut(s) 712
AciI CCGC 1 cut(s) 792
AclWI GGATC 2 cut(s) 147, 292
AcoI YGGCCR 1 cut(s) 310
AgsI TTSAA 4 cut(s) 47, 212, 359, 622
AjnI CCWGG 2 cut(s) 79, 567
AloI GAACNNNNNNTCC 2 cut(s) 438, 470
AluBI AGCT 5 cut(s) 460, 747, 773, 779, 837
AluI AGCT 5 cut(s) 460, 747, 773, 779, 837
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 2 cut(s) 147, 292
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 2 cut(s) 663, 770
Asp700I GAANNNNTTC 1 cut(s) 51
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 2 cut(s) 132, 161
AsuC2I CCSGG 2 cut(s) 136, 137
AsuHPI GGTGA 2 cut(s) 80, 659
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 2 cut(s) 132, 161
BaeI ACNNNNGTAYC 2 cut(s) 27, 60
BalI TGGCCA 1 cut(s) 312
BbsI GAAGAC 2 cut(s) 200, 345
BbvI GCAGC 2 cut(s) 675, 782
BccI CCATC 2 cut(s) 59, 403
BceAI ACGGC 3 cut(s) 129, 481, 791
BciT130I CCWGG 2 cut(s) 81, 569
BcnI CCSGG 2 cut(s) 136, 137
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 1 cut(s) 528
BfmI CTRYAG 1 cut(s) 492
BglII AGATCT 1 cut(s) 539
BisI GCNGC 3 cut(s) 664, 771, 792
BlsI GCNGC 3 cut(s) 665, 772, 793
Bme1390I CCNGG 4 cut(s) 81, 136, 137, 569
Bme18I GGWCC 2 cut(s) 132, 161
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 2 cut(s) 132, 161
BmiI GGNNCC 3 cut(s) 133, 134, 455
BmrFI CCNGG 4 cut(s) 81, 136, 137, 569
BmsI GCATC 1 cut(s) 713
BpiI GAAGAC 2 cut(s) 200, 345
BpmI CTGGAG 2 cut(s) 318, 440
BpuEI CTTGAG 2 cut(s) 426, 528
BpuMI CCSGG 2 cut(s) 136, 137
BsaJI CCNNGG 2 cut(s) 135, 568
BsaXI ACNNNNNCTCC 4 cut(s) 369, 399, 438, 468
Bse1I ACTGG 2 cut(s) 320, 395
Bse3DI GCAATG 1 cut(s) 732
BseBI CCWGG 2 cut(s) 81, 569
BseDI CCNNGG 2 cut(s) 135, 568
BseGI GGATG 2 cut(s) 70, 704
BseMI GCAATG 1 cut(s) 732
BseNI ACTGG 2 cut(s) 320, 395
BseXI GCAGC 2 cut(s) 675, 782
BseYI CCCAGC 1 cut(s) 747
Bsh1236I CGCG 1 cut(s) 712
BshFI GGCC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 1 cut(s) 136
BslFI GGGAC 2 cut(s) 118, 597
BsmAI GTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 118, 597
BsmI GAATGC 2 cut(s) 529, 601
BsnI GGCC 1 cut(s) 312
BsoBI CYCGRG 1 cut(s) 135
Bsp143I GATC 3 cut(s) 139, 284, 539
BspACI CCGC 1 cut(s) 792
BspANI GGCC 1 cut(s) 312
BspFNI CGCG 1 cut(s) 712
BspLI GGNNCC 3 cut(s) 133, 134, 455
BspPI GGATC 2 cut(s) 147, 292
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 2 cut(s) 320, 395
BssECI CCNNGG 2 cut(s) 135, 568
BssMI GATC 3 cut(s) 139, 284, 539
Bst2UI CCWGG 2 cut(s) 81, 569
Bst4CI ACNGT 3 cut(s) 349, 692, 831
Bst6I CTCTTC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 768
BstF5I GGATG 2 cut(s) 70, 704
BstFNI CGCG 1 cut(s) 712
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 3 cut(s) 142, 287, 542
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 3 cut(s) 139, 284, 539
BstMWI GCNNNNNNNGC 3 cut(s) 361, 638, 776
BstNI CCWGG 2 cut(s) 81, 569
BstNSI RCATGY 2 cut(s) 368, 770
BstSCI CCNGG 4 cut(s) 79, 134, 135, 567
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 1 cut(s) 712
BstV1I GCAGC 2 cut(s) 675, 782
BstV2I GAAGAC 2 cut(s) 200, 345
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 2 cut(s) 320, 575
BstYI RGATCY 1 cut(s) 539
BsuRI GGCC 1 cut(s) 312
BtsCI GGATG 2 cut(s) 70, 704
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 3 cut(s) 313, 697, 801
Cac8I GCNNGC 1 cut(s) 768
CfoI GCGC 1 cut(s) 714
Cfr13I GGNCC 2 cut(s) 132, 161
Cfr9I CCCGGG 1 cut(s) 135
CviAII CATG 6 cut(s) 128, 203, 234, 365, 735, 767
CviJI RGCY 9 cut(s) 312, 394, 454, 460, 561, 747, 773, 779, 837
CviKI_1 RGCY 9 cut(s) 312, 394, 454, 460, 561, 747, 773, 779, 837
DpnI GATC 3 cut(s) 141, 286, 541
DpnII GATC 3 cut(s) 139, 284, 539
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 132, 161
Eco88I CYCGRG 1 cut(s) 135
EcoO109I RGGNCCY 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 79, 567
FaeI CATG 6 cut(s) 131, 206, 237, 368, 738, 770
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 2 cut(s) 118, 597
FatI CATG 6 cut(s) 127, 202, 233, 364, 734, 766
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 664, 771, 792
FokI GGATG 2 cut(s) 77, 691
Fsp4HI GCNGC 3 cut(s) 664, 771, 792
FspBI CTAG 1 cut(s) 528
GlaI GCGC 1 cut(s) 713
GluI GCNGC 3 cut(s) 664, 771, 792
GsaI CCCAGC 1 cut(s) 751
GsuI CTGGAG 2 cut(s) 318, 440
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 136
HhaI GCGC 1 cut(s) 714
Hin1II CATG 6 cut(s) 131, 206, 237, 368, 738, 770
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HinfI GANTC 3 cut(s) 153, 180, 437
HpaII CCGG 1 cut(s) 136
HphI GGTGA 2 cut(s) 80, 659
Hpy166II GTNNAC 3 cut(s) 239, 388, 501
Hpy188I TCNGA 6 cut(s) 99, 185, 408, 421, 594, 744
Hpy188III TCNNGA 6 cut(s) 89, 335, 370, 443, 614, 681
Hpy8I GTNNAC 3 cut(s) 239, 388, 501
HpyAV CCTTC 3 cut(s) 55, 427, 631
HpyCH4III ACNGT 3 cut(s) 349, 692, 831
HpyCH4V TGCA 5 cut(s) 9, 294, 632, 725, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 361, 638, 776
Hsp92II CATG 6 cut(s) 131, 206, 237, 368, 738, 770
HspAI GCGC 1 cut(s) 712
KflI GGGWCCC 1 cut(s) 132
Kzo9I GATC 3 cut(s) 139, 284, 539
LmnI GCTCC 3 cut(s) 337, 459, 751
Lsp1109I GCAGC 2 cut(s) 675, 782
LweI GCATC 1 cut(s) 713
MaeI CTAG 1 cut(s) 528
MalI GATC 3 cut(s) 141, 286, 541
MboI GATC 3 cut(s) 139, 284, 539
MboII GAAGA 6 cut(s) 59, 200, 200, 350, 434, 770
MflI RGATCY 1 cut(s) 539
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 3 cut(s) 197, 289, 381
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 162
MnlI CCTC 2 cut(s) 174, 393
Mox20I TGGCCA 1 cut(s) 312
MroXI GAANNNNTTC 1 cut(s) 51
MscI TGGCCA 1 cut(s) 312
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 3 cut(s) 27, 258, 318
Msp20I TGGCCA 1 cut(s) 312
MspI CCGG 1 cut(s) 136
MspR9I CCNGG 4 cut(s) 81, 136, 137, 569
Mva1269I GAATGC 2 cut(s) 529, 601
MvaI CCWGG 2 cut(s) 81, 569
MvnI CGCG 1 cut(s) 712
MwoI GCNNNNNNNGC 3 cut(s) 361, 638, 776
NciI CCSGG 2 cut(s) 136, 137
NdeII GATC 3 cut(s) 139, 284, 539
NlaIII CATG 6 cut(s) 131, 206, 237, 368, 738, 770
NlaIV GGNNCC 3 cut(s) 133, 134, 455
NspI RCATGY 2 cut(s) 368, 770
PaeI GCATGC 1 cut(s) 770
PctI GAATGC 2 cut(s) 529, 601
PdmI GAANNNNTTC 1 cut(s) 51
PfeI GAWTC 2 cut(s) 180, 437
PkrI GCNGC 3 cut(s) 665, 772, 793
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 132
Psp6I CCWGG 2 cut(s) 79, 567
PspFI CCCAGC 1 cut(s) 747
PspGI CCWGG 2 cut(s) 79, 567
PspN4I GGNNCC 3 cut(s) 133, 134, 455
PspPI GGNCC 2 cut(s) 132, 161
PspPPI RGGWCCY 1 cut(s) 132
PsuI RGATCY 1 cut(s) 539
RseI CAYNNNNRTG 3 cut(s) 27, 258, 318
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 3 cut(s) 664, 771, 792
Sau3AI GATC 3 cut(s) 139, 284, 539
Sau96I GGNCC 2 cut(s) 132, 161
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 4 cut(s) 81, 136, 137, 569
SetI ASST 9 cut(s) 82, 217, 462, 649, 720, 749, 775, 781, 839
SfaNI GCATC 1 cut(s) 713
SfcI CTRYAG 1 cut(s) 492
SinI GGWCC 2 cut(s) 132, 161
SmaI CCCGGG 1 cut(s) 137
SmiMI CAYNNNNRTG 3 cut(s) 27, 258, 318
SmlI CTYRAG 2 cut(s) 441, 543
SmoI CTYRAG 2 cut(s) 441, 543
SphI GCATGC 1 cut(s) 770
Sse9I AATT 3 cut(s) 197, 289, 381
SsiI CCGC 1 cut(s) 792
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 4 cut(s) 79, 134, 135, 567
TaaI ACNGT 3 cut(s) 349, 692, 831
TasI AATT 3 cut(s) 197, 289, 381
TauI GCSGC 1 cut(s) 794
TfiI GAWTC 2 cut(s) 180, 437
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 3 cut(s) 320, 697, 801
TseI GCWGC 2 cut(s) 663, 770
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 214
TspMI CCCGGG 1 cut(s) 135
TspRI CASTG 3 cut(s) 320, 697, 801
VpaK11BI GGWCC 2 cut(s) 132, 161
XceI RCATGY 2 cut(s) 368, 770
XmaI CCCGGG 1 cut(s) 135
XmiI GTMKAC 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 51
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.